欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2012, Vol. 38 ›› Issue (07): 1205-1211.doi: 10.3724/SP.J.1006.2012.01205

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦地方品种高分子量谷蛋白亚基多样性分析

徐鑫1,2,**，李小军1,3,**，张玲丽1,4，李秀全1，杨欣明1，李立会1,*   

  1. 1中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程，北京 100081；2新乡学院生命科学与技术系，河南新乡 453003；3河南科技学院生命学院，河南新乡 453003；4西北农林科技大学农学院，陕西杨凌 712100
  • 收稿日期:2011-11-24 修回日期:2012-02-23 出版日期:2012-07-12 网络出版日期:2012-05-11
  • 通讯作者: 李立会, E-mail: lilihui@caas.net.cn, Tel: 010-62186670 ** 同等贡献(Contributed equally to this work)
  • 基金资助:

    本研究由国家重点基础研究发展计划(973计划)项目(200611B100201)资助。

Genetic Diversity of High-Molecular-Weight Glutenin Subunit Composition in Chinese Wheat Landraces

XU Xin1,2,**,LI Xiao-Jun1,3,**,ZHANG Ling-Li1,4,LI Xiu-Quan1,YANG Xin-Ming1,LI Li-Hui1,*   

  1. 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facilities for Crop Genetic Resources and Improvement, Beijing 100081, China; 2Department of Life Sciences and Technology, Xinxiang University, Xinxiang 453003, China; 3School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China; 4College of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China
  • Received:2011-11-24 Revised:2012-02-23 Published:2012-07-12 Published online:2012-05-11
  • Contact: 李立会, E-mail: lilihui@caas.net.cn, Tel: 010-62186670 ** 同等贡献(Contributed equally to this work)

PDF

1730

被引次数

12

摘要： 采用SDS-PAGE方法，对我国9个麦区的76份代表性地方品种的高分子量谷蛋白亚基(HMW-GS)组成比较分析，并探讨其与环境因素(平均海拔、年平均降雨量和年平均温度)的相关性。结果表明，25.0%的品种具有异质性，分别包含2~4种不同HMW-GS组合；在Glu-1位点共检测到14个等位变异，其中Glu-A1、Glu-B1和Glu-D1等位变异数分别为2、7和5；发现了3个新等位变异，包括Glu-B1位点2个和Glu-D1位点1个。所有等位变异构成16种不同的亚基组合类型，以(N, 7+8, 2+12)为主，频率为69.7%。在Glu-1位点上，不同麦区遗传多样性分布存在一定的不均衡性，年平均降雨量和年平均温度与麦区多样性指数呈负相关。推测环境压力可能是地方品种多样性地区分化的重要因素。

关键词: 小麦地方品种, 高分子量谷蛋白亚基, 多样性, 环境因素

Abstract: The high-molecular-weight glutenin subunit (HMW-GS) composition of 76 representative accessions of wheat landraces, collected from nine agro-ecological zones in China, were examined by sodium-dodecyl-sulphate polyacrylamide-gel electrophoresis (SDS-PAGE). The correlation between diversity indexes at Glu-1 locus and altitude, mean annual precipitation, or mean annual temperature was also analyzed. The results indicated that 19 accessions (25.0%) were heterogeneous for HMW glutenin subunit composition, and contained 2–4 HMW-GS compositions generally. At Glu-1 locus, a total of 14 different glutenin alleles were observed and the number of alleles at Glu-A1, Glu-B1, and Glu-D1 was 2, 7, and 5, respectively. Three novel alleles were identified, consisting of two alleles at Glu-B1 and one allele at Glu-D1 locus. The 14 alleles resulted in 16 different HMW subunit combinations, and the combination (null, 7+8, 2+12) was the major type with the frequency of 69.7%. The genetic diversity indexes for HMW glutenin subunits varied among agro-ecological zones, and were negatively correlated with mean annual precipitation and mean annual temperature. Environmental stress is speculated to be an important factor for the differentiation of diversity in wheat landraces across regions.

Key words: Wheat landrace, HMW-GS, Diversity, Environmental factor

[1]Tian Q Z, Zhou R H, Jia J Z. Genetic diversity trend of common wheat (Triticum aestivum L.) in China revealed with AFLP markers. Genet Resour Crop Evol, 2005, 52: 325–331

[2]Reif J C, Zhang P, Dreisigacker S, Warburton M L, van Ginkel M, Hoisington D, Bohn M, Melchinger A E. Wheat genetic diversity trends during domestication and breeding. Theor Appl Genet, 2005, 110: 859–864

[3]Fu Y, Peterson G W, Richards K W, Somers D, DePauw R M, Clarke J M. Allelic reduction and genetic shift in the Canadian hard red spring wheat germplasm released from 1845 to 2004. Theor Appl Genet, 2005, 110: 1505–1516

[4]Zhu Y Y, Chen H R, Fan J H, Wang Y Y, Li Y, Chen J B, Fan J X, Yang S S, Hu L P, Leung H, Mew T W, Teng P S, Wang Z H, Mundt C C. Genetic diversity and disease control in rice. Nature, 2000, 406: 718–722

[5]Gregová E, Hermuth J, Kraic J, Dotla?il L. Protein heterogeneity in European wheat landraces and obsolete cultivars. Genet Resour Crop Evol, 1999, 46: 521–528

[6]Cai S, Bai G H, Zhang D. Quantitative trait loci for aluminum resistance in Chinese wheat landrace FSW. Theor Appl Genet, 2008, 117: 49–56

[7]Du X-Y(杜小燕), Hao C-Y(郝晨阳), Zhang X-Y(张学勇), Ma Z-Q(马正强), You G-X(游光霞), Wang L-F(王兰芬), Dong Y-C(董玉琛). Genetic diversity of waxy gene in Chinese wheat landraces. Acta Agron Sin (作物学报), 2007, 33(3): 503–506 (in Chinese with English abstract)

[8]Dong Y-C(董玉琛), Zheng D-S(郑殿升). Wheat Genetic Resources in China (中国小麦遗传资源). Beijing: China Agriculture Press, 2000 (in Chinese)

[9]Harlan J R. Our vanishing genetic resources. Science, 1975, 188: 618–621

[10]Zhuang Q-S(庄巧生). Chinese Wheat Improvement and Pedigree Analysis (中国小麦品种改良及系谱分析). Beijing: China Agriculture Press, 2003 (in Chinese)

[11]Hirano R, Kikuchi A, Kawase M, Watanabe K N. Evaluation of genetic diversity of bread wheat landraces from Pakistan by AFLP and implications for a future collection strategy. Genet Resour Crop Evol, 2008, 55: 1007–1015

[12]Nakamura H. Genetic diversity of high-molecular-weight glutenin subunit compositions in landraces of hexaploid wheat from Japan. Euphytica, 2001, 120: 227–234

[13]Liu Y, Xiong Z Y, He Y G, Shewry P R, He G Y. Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province. Genet Resour Crop Evol, 2007, 54: 865–874

[14]Ruiza M, Rodriguez-Quijanob M, Metakovskyb E V, Vazquezb J F, Carrillo J M. Polymorphism, variation and genetic identity of Spanish common wheat germplasm based on gliadin alleles. Field Crop Res, 2002, 79: 185–196

[15]He D, Li H, Xu S, Duan X, Zhou Y, Li L. Reaction to powdery mildew and stripe rust in related species and landraces of wheat. Genet Resour Crop Evol, 2007, 54: 213–219

[16]Bajracharya J, Rana R B, Gauchan D, Sthapit B R, Jarvis D I, Witcombe J R. Rice landrace diversity in Nepal. Socio-economic and ecological factors determining rice landrace diversity in three agro-ecozones of Nepal based on farm surveys. Genet Resour Crop Evol, 2010, 57: 1013–1022

[17]Jaradat A A. Grain protein variability among populations of wild barley (Hordeum spontaneum C. Koch.) from Jordan. Theor Appl Genet, 1991, 83: 164–168

[18]Li Y C, Fahima T, Korol A B, Peng J H, Röder M S, Kirzhner V, Beiles A, Nevo E. Microsatellite diversity correlated with ecological-edaphic and genetic factors in three microsites of wild emmer wheat in north Israel. Mol Biol Evol, 2000, 17: 851–862

[19]Lázaro A, Ruiz M, Rosa L, Martín I. Relationships between agro/morphological characters and climatic parameters in Spanish landraces of lentil (Lens culinaris Medik.). Genet Resour Crop Evol, 2001, 48: 239–249

[20]Zhang L-L(张玲丽), Li X-Q(李秀全), Yang X-M(杨欣明), Li H-J(李洪杰), Wang H(王辉), Li L-H(李立会). High-molecular-weight glutenin subunit composition of Chinese wheat germplasm. Sci Agric Sin (中国农业科学), 2006, 39(12): 2406–2414 (in Chinese with English abstract)

[21]Payne P I, Lawrence G J. Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for high-molecular-weight subunits of glutein in hexaploid wheat. Cereal Res Commun, 1983, 11: 29–35

[22]Nei M. Analysis of gene diversity in subdivided populations. Proc Nat Acad Sci USA, 1973, 70: 3321–3323

[23]Peña R J, Zarco-Hernandez J, Mujeeb-Kazi A. Glutenin subunit compositions and breading-making quality characteristics of synthetic hexaploid wheats derived from Triticum turgidum × Triticum tauschii (Coss.) Schmal crosses. J Cereal Sci, 1995, 21: 15–23

[24]Tang Y-L(汤永禄), Yang W-Y(杨武云), Tian J-C(田纪春), Li J(李俊), Chen F(陈放). Effect of HMW-GS 6+8 and 1.5+10 on wheat quality traits in synthetic hexaploid wheat. Agric Sci China (中国农业科学), 2008, 41(11): 3465–3476 (in Chinese with English abstract)

[25]Ruiz M, Metakovsky E V, Rodriguez-Quijano M, Vazquez J F, Carrillo J M. Assessment of storage protein variation in relation to some morphological characters in a sample of Spanish landraces of common wheat (Triticum aestivum L. ssp. aestivum). Genet Resour Crop Evol, 2002, 49: 371–382

[26]Wei Y-M(魏育明), Zheng Y-L(郑有良), Liu D-C(刘登才), Zhou Y-H(周永红), Lan X-J(兰秀锦). Genetic diversity of Gli-1, Gli-2 and Glu-1 alleles in Sichuan wheat landraces. Acta Bot Sin (植物学报), 2000, 42(5): 496–501 (in Chinese with English abstract)

[27]Zhang X-Y(张学勇), Pang B-S(庞斌双), You G-X(游光霞), Wang L-F(王兰芬), Jia J-Z(贾继增), Dong Y-C(董玉琛). Allelic variation and genetic diversity at Glu-1 loci in Chinese wheat (Triticum aestivum L.) germplasms. Agric Sci China (中国农业科学), 2002, 35(11): 1302–1310 (in Chinese with English abstract)

[28]Amirouche R, Misset M T. Hordein polymorphism in diploid and tetraploid Mediterranean populations of the Hordeum murinum L. complex. Plant Syst Evol, 2003, 242: 83–99

[29]Nevo E, Payne P I. Wheat storage proteins: diversity of HMW glutenin subunits in wild emmer from Israel. Theor Appl Genet, 1987, 74: 827–836

[30]Allard R W. Genomic basis of the evolution of adaptedness in plants. Euphytica, 1996, 92: 1–11

[31]Sertse D, Gailing O, Eliades N, Finkeldey R. Anthropogenic and natural causes in?uencing population genetic structure of Juniperus procera Hochst. ex Endl. in the Ethiopian highlands. Genet Resour Crop Evol, 2011, 58: 849–859

[32]Rana R B, Garforth C, Sthapit B, Jarvis D. In?uence of socio-economic and cultural factors in rice varietal diversity management on-farm in Nepal. Agric Human Values, 2007, 24: 461–472
[1] 杨扬, 常诗惠, 田红丽, 易红梅, 王璐, 任洁, 范亚明, 刘亚维, 王凤格, 赵久然. 不同生态区国审玉米品种的遗传多样性分析[J]. 作物学报, 2026, 52(5): 1352-1364.
[2] 徐建霞, 丁延庆, 曹宁, 程斌, 高旭, 李文贞, 王若若, 王磊, 张立异. 397份高粱种质资源在贵州表型多样性分析及综合评价[J]. 作物学报, 2026, 52(4): 1073-1087.
[3] 田春艳, 陆鑫, 吴才文, 徐超华, 刘家勇, 边芯, 桃联安. 基于荧光SSR的甘蔗创新种质遗传多样性分析及育种潜力评估[J]. 作物学报, 2026, 52(4): 1057-1072.
[4] 侯洁, 付朵朵, 武海峰, 郝宇琼, 郑兴卫, 武棒棒, 周凯, 李晓华, 郑军, 赵佳佳. 山西省小麦地方品种的染色体多样性及遗传效应分析[J]. 作物学报, 2026, 52(3): 746-763.
[5] 梅飘, 刘丁丁, 叶圆圆, 张晨禹, 丁诗琦, 李亚奇, 王培鑫, 梅菊芬, 马春雷. 基于茶树液相功能芯片的白化茶树资源遗传多样性分析[J]. 作物学报, 2025, 51(9): 2358-2370.
[6] 王天译, 杨绣娟, 赵佳佳, 郝宇琼, 郑兴卫, 武棒棒, 李晓华, 郝水源, 郑军. 山西小麦醇溶蛋白多样性及其对面粉品质效应研究[J]. 作物学报, 2025, 51(7): 1784-1800.
[7] 旺姆, 卓嘎, 扎桑, 西若曲宗, 达瓦顿珠, 郭刚刚, 张京, 卓嘎, 伦珠朗杰. 基于6个表型性状的青稞种质遗传多样性分析及综合评价[J]. 作物学报, 2025, 51(6): 1526-1537.
[8] 徐晓伟, 冯晶, 王凤涛, 童朝阳, 张建周, 李春盈, 蔺瑞明. 小麦地方品种蚕老麦成株抗条锈病QTL定位[J]. 作物学报, 2025, 51(11): 2933-2943.
[9] 王浩辰, 王克晶, 韩娟, 李向华. 东南沿海短绒野大豆两种代表性生境自然种群的空间遗传结构特征:种群内取样策略研究[J]. 作物学报, 2025, 51(11): 2875-2885.
[10] 陈佳伟, 林艳, 张明星, 周诗晶, 饶力群, 周池, 李鑫. 贝莱斯芽孢杆菌YCH92对棉花根际土壤微生物群落及棉花产量的影响[J]. 作物学报, 2025, 51(10): 2821-2835.
[11] 张红岩, 敏玉霞, 滕长才, 彭小星, 陈志凯, 周仙莉, 娄树宝, 刘玉皎. 利用130K液相芯片分析中国蚕豆种质资源遗传多样性[J]. 作物学报, 2024, 50(8): 1989-2000.
[12] 李长喜, 董占鹏, 关永虎, 刘金伟, 李航, 梅拥军. 南疆陆地棉农艺性状与皮棉产量性状的遗传贡献及决策系数分析[J]. 作物学报, 2024, 50(6): 1486-1502.
[13] 柯会锋, 苏红梅, 孙正文, 谷淇深, 杨君, 王国宁, 徐东永, 王洪这, 吴立强, 张艳, 张桂寅, 马峙英, 王省芬. 棉花现代品种资源产量与纤维品质性状鉴定及分子标记评价[J]. 作物学报, 2024, 50(2): 280-293.
[14] 苏一钧, 赵路宽, 唐芬, 戴习彬, 孙亚伟, 周志林, 刘亚菊, 曹清河. 378份甘薯引进种遗传多样性及群体结构分析[J]. 作物学报, 2023, 49(9): 2582-2593.
[15] 徐高峰, 申时才, 张付斗, 杨韶松, 金桂梅, 郑凤萍, 温丽娜, 张云, 吴冉迪. 土壤微生物对长雄野生稻及其化感潜力后代抑草作用的影响[J]. 作物学报, 2023, 49(9): 2562-2571.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2