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作物学报 ›› 2012, Vol. 38 ›› Issue (11): 2034-2041.doi: 10.3724/SP.J.1006.2012.02034

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦生长素结合基因TaABP1-D的克隆、功能标记开发及其与株高的关联

乔麟轶1,**，张磊1,**，张文萍1，赵光耀2，王玺1,*，贾继增2,*   

  1. 1沈阳农业大学农学院, 辽宁沈阳 110866; 2中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程 / 农业部作物基因资源与种质创制重点实验室, 北京 100081
  • 收稿日期:2012-03-22 修回日期:2012-06-15 出版日期:2012-11-12 网络出版日期:2012-09-10
  • 通讯作者: 王玺, E-mail: wxi2000@126.com; 贾继增, E-mail: jzjia@mail.caas.net.cn
  • 基金资助:

    本研究由国家转基因生物新品种培育科技重大专项(2011ZX08009-001), 教育部博士点专项基金(20092103120010)和辽宁省科技厅博士启动基金(20091074)资助。

Molecular Cloning and Development of a Functional Marker of TaABP1-D Gene Associated with Plant Height in Bread Wheat

QIAO Lin-Yi1,**,ZHANG Lei1,**,ZHANG Wen-Ping1,ZHAO Guang-Yao2,WANG Xi1,*,JIA Ji-Zeng2,*   

  1. 1 College of Agronomy, Shenyang Agricultural University, Shenyang 110866, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Gene Resources & Germplasm Enhancement, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2012-03-22 Revised:2012-06-15 Published:2012-11-12 Published online:2012-09-10
  • Contact: 王玺, E-mail: wxi2000@126.com; 贾继增, E-mail: jzjia@mail.caas.net.cn

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1844

被引次数

6

摘要：

生长素在植物生长发育过程中通过建立浓度梯度来调控植物的株型。ABP1 (auxin binding protein)作为生长素受体, 在质膜上相关生长素响应活动中起着重要的作用。本研究从普通小麦中国春基因组数据库中分离了TaABP1基因的基因组序列, 根据基因组间序列差异将 TaABP1 定位于小麦第5同源群。在中国春中克隆得到TaABP1-D基因的gDNA和cDNA序列。TaABP1-D基因的开放阅读框为1 887 bp, 编码205个氨基酸, 含有ABP1蛋白典型的内质网滞留信号KDEL及Box区域。表达分析表明, TaABP1-D在普通小麦中国春拔节期的根、茎基部、茎上部和叶尖均有表达, 相对表达量为叶尖>茎上部>根>茎基部, 与小麦的株型发育关系密切。系统发育分析表明, ABP1基因在植物中较为保守, TaABP1-D是水稻OsABP1的直向同源基因。针对TaABP1-D基因上游调控区重复序列差异(GT)6/5开发了一个SSR标记, 该标记在W7984× Opata85重组自交系(RIL)群体中对株高的表型变异解释率为9.7%, 是一个与株高极显著关联的功能标记; 其中对应高秆类型的等位变异属野生种特有, 在栽培种中被淘汰, 推测TaABP1-D基因在小麦驯化过程中可能经历了瓶颈效应。

关键词: 小麦, TaABP1-D基因, 同源克隆, 表达分析, 功能标记, 相关性分析

Abstract:

AUXIN BINDING PROTEIN1 (ABP1) is one of the ?rst characterized proteins that binds auxin. It ful?lls the criteria for an auxin receptor and plays an important role in auxin responses on the plasma membrane. In this study, the genomic sequence of a novel gene, designated TaABP1, isolated from the hexaploid wheat (Triticum aestivum L.) Chinese Spring genome databases. The result of chromosomal location showed that TaABP1 was on homoelogous group 5 of chromosomes in wheat. Then TaABP1-D was cloned in Chinese Spring. Sequence analysis showed that the complete open reading frame (ORF) of TaABP1-D was 1887 bp in length, encoding a putative protein composed of 205 amino acids with theendoplasmic reticulum retention sequence Lys-Asp-Glu-Leu (KDEL) and box regions. The expression of TaABP1-D was detected in root, basal stem, upper stem, and leaf tip of wheat at jointing stage with the expression levels as leaf tip > upper stems > root > base stem. Sequence alignment proved that TaABP1-D shared high homology with rice OsABP1 (82% protein sequence identity) and other plant ABPs reported. Using a population of recombinant inbred lines from the cross between W7984 and Opata85, an SSR maker was developed based on (GT)6/5 divergence in the upstream sequence of TaABP1-D. This marker was proved to be highly correlated with plant height, and the phenotypic variation explained was 9.7%. The W7984 type allelic variation for tall plant was specific in wild varieties, and eliminated in cultivated varieties. This indicates that the favorable variation of TaABP1-D has been fixed in cultivated varieties of wheat, and a bottleneck effect might occur during the domestication of wheat.

Key words: Wheat, TaABP1-D, Homologous cloning, Expression analysis, Functional marker, Association analysis

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