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作物学报 ›› 2025, Vol. 51 ›› Issue (12): 3133-3143.doi: 10.3724/SP.J.1006.2025.52012

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

抗白叶枯病广亲和恢复系的创制及杂交育种应用研究

谢留杰, 段敏1, 杨勇2, 潘晓飚1, 马伯军3, 黄善军1,*(), 陈析丰3,*()   

  1. 1台州市农业科学研究院, 浙江台州 318000
    2浙江省农业科学院, 浙江杭州 310000
    3浙江师范大学生命科学学院, 浙江金华 321004
  • 收稿日期:2025-04-24 接受日期:2025-09-10 出版日期:2025-12-12 网络出版日期:2025-09-23
  • 通讯作者: *黄善军, E-mail: 406716425@qq.com;陈析丰, E-mail:xfchen@zjnu.cn
  • 基金资助:
    本研究由浙江省基础公益研究计划项目(LTGN24C130002);本研究由浙江省基础公益研究计划项目(LQ24C060003);浙江省农业新品种选育重大科技专项(2021C02063-5-5)

Development of wide-compatibility restorer lines resistant to bacterial blight and their application in rice hybrid breeding

XIE Liu-Jie, DUAN Min1, YANG Yong2, PAN Xiao-Biao1, MA Bo-Jun3, HUANG Shan-Jun1,*(), CHEN Xi-Feng3,*()   

  1. 1Taizhou Research Academy of Agriculture Sciences, Taizhou 318000, Zhejiang, China
    2Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, Zhejiang, China
    3College of Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China
  • Received:2025-04-24 Accepted:2025-09-10 Published:2025-12-12 Published online:2025-09-23
  • Contact: *E-mail: 406716425@qq.com;E-mail: xfchen@zjnu.cn
  • Supported by:
    Basic Public Welfare Research Project of Zhejiang Province(LTGN24C130002);Basic Public Welfare Research Project of Zhejiang Province(LQ24C060003);Zhejiang Province Major Science and Technology Special Project for the Breeding of New Agricultural Varieties(2021C02063-5-5)

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摘要： 以镇恢084 (Xa7)、IRBB21 (Xa21)、CBB23 (Xa23)、IRBB27 (Xa27)为供体亲本, 开发和利用基因功能性分子标记, 将目标基因导入并聚合到广亲和恢复系F7540中; 采用9个白叶枯病生理小种对改良的恢复系及其轮回亲本进行抗性鉴定; 选择6个抗性改良的恢复系分别与3个籼稻(不育系泰1S、荃9311A、华浙2A)和3个粳稻不育系(春江16A、春江88A、华中2A)杂交, 用菌株浙173和PXO99接种不育系、恢复系和杂交组合, 同时考察亲本和杂交组合的农艺性状。结果表明, 通过多代选育, 共获得9个不同抗性基因组合的导入系; 其中, 恢复系F7540-Xa7- Xa23-Xa27的抗病能力最强, 对9个生理小种均表现为高抗, F7540-Xa7-Xa23和F7540-Xa23对9个生理小种表现为抗或高抗, F7540-Xa7-Xa21和F7540-Xa7-Xa21-Xa27对8个生理小种表现为抗病, F7540-Xa7和F7540-Xa21分别抗7个和2个生理小种, 而F7540-Xa27对所有供试小种均表现为感病。杂交组合接种浙173和PXO99两个生理小种的结果显示, F7540-Xa23和F7540-Xa7-Xa23配组的所有组合均表现为抗病, F7540-Xa7-Xa21与3个粳稻不育系配组的组合均表现为抗病, F7540-Xa21与不育系荃9311A配制的组合表现为抗病。综合考查抗性及农艺性状发现, F7540-Xa23与不育系春江88A和荃9311A配组的杂交组合抗病性好、农艺性状优异, 有较高的育种利用价值。

关键词: 抗性基因, 白叶枯病, 基因聚合, 广亲和恢复系, 杂交育种

Abstract:

Zhenghui 084 (Xa7), IRBB21 (Xa21), CBB23 (Xa23), and IRBB27 (Xa27) were used as donor parents to develop gene-specific functional molecular markers and introduce target resistance genes into the wide-compatibility restorer line F7540 through gene pyramiding. The resulting improved restorer lines, along with recurrent parents, were inoculated with nine races of Xanthomonas oryzae pv. oryzae (Xoo). Six improved resistant restorer lines were then crossed with three indica male sterile lines (Tai 1S, Quan 9311A, Huazhe 2A) and three japonica male sterile lines (Chunjiang 16A, Chunjiang 88A, Huazhong 2A). Both hybrid combinations and parental lines were inoculated with two representative Xoo races, Zhe173 and PXO99, and agronomic traits were also evaluated. The results showed that nine restorer lines carrying various resistance genes were successfully developed through multi-generation selection. Among them, F7540-Xa7-Xa23-Xa27 exhibited high resistance to all nine Xoo races. F7540-Xa7-Xa23 and F7540-Xa23 also showed resistance or high resistance to all nine races. Lines F7540-Xa7-Xa21 and F7540-Xa7-Xa21-Xa27 were resistant to eight races, while F7540-Xa7 and F7540-Xa21 were resistant to seven and two races, respectively. Notably, F7540-Xa27 showed no resistance to any of the tested races. In the hybrid inoculation tests with Zhe173 and PXO99, all combinations involving F7540-Xa23 and F7540-Xa7-Xa23 showed disease resistance. Hybrids derived from F7540-Xa7-Xa21 crossed with japonica male sterile lines also exhibited resistance. Additionally, the combination of F7540-Xa21 and Quan 9311A was resistant to disease. Comprehensive evaluation of resistance and agronomic performance indicated that the hybrid combinations of F7540-Xa23 with Chunjiang 88A and Quan 9311A possessed excellent disease resistance and favorable agronomic traits, demonstrating high breeding potential.

Key words: resistance genes, bacterial blight, gene pyramiding, wide-compatibility restorer line, hybrid breeding

表1

抗性基因功能分子标记的序列"

基因名称
Gene name		 引物名称
Primer name		 序列
Sequence (5′-3′)		 抗病/感病
Resistant (R) /susceptible (S)		 产物片段
Product size (bp)
Xa7 M6 F GGGAGAAATTGGCCCCAGTTAGAGAA R 306
M6 R GCTTCACTTGATCACTGCTGTTTG S 531
Xa23 P23 F TGCCACAGCCAAAGTATGATGCT R 474
P23 R TTGGCCCCTGCAGAGAAGGC S 600
RM206 F CCCATGCGTTTAACTATTCT R 117
RM206 R CGTTCCATCGATCCGTATGG S 173
Xa27 G27 F TAGTGTCTAAATACAGGGACT R 175
G27 R GAGTACTTTGCTCTGATGCTC S 149
Xa21 Xa21-IF1 GCTAAATATAGCTAGTTCATAGAG R 169
Xa21-IR1 TTACTCAAATTTGATCCTTCTG S 142
Xa21-IF2 ACATAATTATACATCCAAGTTCT R 155
Xa21-IR2 CACTAGAATTCCATAGCTGTAA S 133

图1

Xa21基因功能分子标记的设计 M: marker; 1: IRBB21; 2: IRBB5; 3: 镇恢084; 4: CBB23; 5: IRBB27; 6: F7540; 7: 明恢63; 8: 9311; 9: 日本晴。"

图2

分子标记检测抗性改良恢复系基因型 A: 分子标记P23检测Xa23位点; B: 分子标记M6检测Xa7位点; C: 分子标记G27检测Xa27位点; D: 分子标记RM206检测Xa23位点: E: 分子标记Xa21-IF1检测Xa21位点; F: 分子标记Xa21-IF2检测Xa21位点。M: marker; 1~9分别为F7540-Xa7 (1)、F7540-Xa21 (2)、F7540-Xa23 (3)、F7540-Xa27 (4)、F7540-Xa7-Xa23 (5)、F7540-Xa7-Xa21 (6)、F7540-Xa21-Xa27 (7)、F7540-Xa7-Xa21-Xa27 (8)、F7540-Xa7-Xa21-Xa23 (9)共9个不同抗性基因的改良恢复系; 10: F7540。"

表2

抗性改良恢复系对9个白叶枯病生理小种的抗性反应"

生理小种
Physiological races		 改良恢复系Improved resistant restorer lines F7540 金刚30
Jingang 30
F7540-Xa7 F7540-
Xa21		 F7540-
Xa23		 F7540-
Xa27		 F7540-Xa7-Xa23 F7540-Xa7-Xa21 F7540-Xa21-Xa27 F7540-Xa7-
Xa21-Xa27		 F7540-Xa7-
Xa23-Xa27
台州
Taizhou		 S MR R S HR HR MR HR HR S HS
绍兴
Shaoxing		 R MR HR S HR HR MR HR HR S HS
衢州
Quzhou		 HR R HR MS HR HR R HR HR HS MS
金华2
Jinhua 2		 HR MS HR S HR HR R R HR HS HS
金华3
Jinhua 3		 R S HR S HR R R R HR HS HS
金华12
Jinhua 12		 R S HR S HR R R R HR HS HS
海南
Hainan		 R MS HR MS HR R R R HR S HS
PXO99 S MR HR HS R MS R MR HR HS HS
浙173
Zhe 173		 HR R HR S HR HR MR R HR S HS

图3

改良恢复系对菌株浙173和PXO99的抗性"

表3

改良恢复系的杂交组合对菌株浙173的抗性"

恢复系
Restorer line		 不育系Sterile line
泰1S
Tai 1S
(R)		 华浙2A
Huazhe 2A
(S)		 荃9311A
Quan 9311A (HS)		 春江16A
Chunjiang 16A (R)		 春江88A
Chunjiang 88A (S)		 华中2A
Huazhong 2A (HS)
F7540-Xa7 (HR) R S S R MS MS
F7540-Xa21 (R) MR HS R R HS S
F7540-Xa23 (HR) HR HR R HR R R
F7540-Xa7-Xa21 (HR) HR R R R R R
F7540-Xa7-Xa23 (HR) HR HR HR HR HR R
F7540-Xa21-Xa27 (R) R HS MR R HS MR
F7540 (MS) S HS S MR S S

表4

改良恢复系的杂交组合对菌株PXO99的抗性反应"

恢复系
Restorer line		 不育系Sterile line
泰1S
Tai 1S
(MR)		 华浙2A
Huazhe 2A (HS)		 荃9311A
Quan 9311A (HS)		 春江16A
Chunjiang 16A (MR)		 春江88A
Chunjiang 88A (S)		 华中2A
Huazhong 2A (HS)
F7540-Xa7 (HS) HS HS HS HS S S
F7540-Xa21 (MR) S HS MR HS MR S
F7540-Xa23 (HR) HR R HR HR HR HR
F7540-Xa7-Xa21 (R) MS S S HR R MR
F7540-Xa7-Xa23 (R) MR MR R R R MR
F7540-Xa21-Xa27 (MR) MS S S S S S
F7540 (S) S HS HS S S HS

表5

抗性改良恢复系农艺性状表现"

恢复系
Restorer line		 株高
PH
(cm)		 生育期
GP
(d)		 单株分蘖
PN		 穗长
PL
(cm)		 总粒数
TNP		 实粒数
SNP		 结实率
SF
(%)		 千粒重
TGW
(g)		 单株产量
GY
(g)
F7540-Xa7 127.0 f 128.0 c 10.33 e 23.3 a 308.3 d 266.9 d 86.6 e 24.8 c 34.03 e
F7540-Xa21 108.3 c 125.2 a 8.67 a 24.9 b 273.0 b 224.3 b 82.2 b 26.0 f 32.57 d
F7540-Xa23 107.0 b 126.2 b 10.00 d 24.9 b 223.1 a 173.7 a 77.9 a 25.6 e 24.37 b
F7540-Xa7-Xa21 128.3 g 129.1 d 8.67 a 27.5 f 344.7 g 295.9 g 85.8 d 25.2 d 31.43 c
F7540-Xa7-Xa23 124.0 e 127.5 c 9.67 c 25.6 d 279.8 c 244.8 c 87.5 f 25.6 e 35.43 f
F7540-Xa21-Xa27 114.3 d 129.5 d 9.00 b 25.8 e 332.2 e 290.2 e 87.4 f 23.0 a 38.53 g
F7540 103.0 a 124.4 a 9.00 b 25.3 c 340.7 f 283.9 f 83.3 c 24.2 b 20.40 a

表6

抗性改良恢复系杂交组合的农艺性状分析"

杂交组合
Combination		 株高
PH (cm)		 单株分蘖
PN		 穗长
PL (cm)		 总粒数
TNP		 实粒数
SNP		 结实率
SF (%)		 千粒重
TGW (g)		 单株产量
GY (g)
Tai 1S×F7540-Xa7 121.7 g 17.0 d 24.3 b 248.9 d 197.0 e 79.1 d 23.2 c 40.2 e
Tai 1S×F7540-Xa21 104.3 a 13.3 b 25.4 c 260.7 f 200.5 f 76.9 c 22.8 b 37.6 d
Tai 1S×F7540-Xa23 106.3 b 13.7 c 24.4 b 235.3 b 181.1 c 77.0 c 24.0 e 33.5 b
Tai 1S×F7540-Xa7-Xa21 111.7 c 12.7 a 25.8 d 244.5 c 163.1 b 66.7 a 22.4 a 31.9 a
Tai 1S×F7540-Xa7-Xa23 120.7 f 20.3 f 22.7 a 190.8 a 155.0 a 81.2 e 23.4 d 35.3 c
Tai 1S×F7540-Xa21-Xa27 114.3 d 19.0 e 24.3 b 255.0 e 195.0 d 76.5 c 22.4 a 41.6 f
Tai 1S×F7540 117.0 e 17.0 d 26.7 e 285.8 g 202.7 g 70.9 b 24.2 f 54.1 g
Quan 9311A×F7540-Xa7 129.0 f 11.3 b 33.2 d 287.8 f 192.5 d 66.9 b 28.6 e 39.9 b
Quan 9311A×F7540-Xa21 118.3 b 16.3 e 27.4 b 240.4 b 172.5 a 71.7 c 27.4 b 50.4 d
Quan 9311A×F7540-Xa23 120.3 c 18.7 g 27.5 b 270.9 c 196.4 e 72.5 d 27.6 c 56.7 g
Quan 9311A×F7540-Xa7-Xa21 133.3 g 10.3 a 28.1 c 313.8 g 189.5 c 60.4 a 26.6 a 31.8 a
Quan 9311A×F7540-Xa7-Xa23 122.7 d 15.7 d 25.3 a 223.7 a 184.1 b 82.3 e 28.0 d 45.4 c
Quan 9311A×F7540-Xa21-Xa27 125.3 e 12.7 c 28.0 c 281.9 e 205.1 f 72.8 d 27.6 c 54.4 f
Quan 9311A×F7540 114.3 a 16.7 f 25.2 a 278.3 d 205.2 f 73.7 f 27.5 bc 52.4 e
Huazhe 2A×F7540-Xa7 128.0 f 13.7 a 24.6 b 248.9 c 179.8 b 72.2 c 23.0 d 41.5 c
Huazhe 2A×F7540-Xa21 120.0 c 15.3 b 24.9 c 224.2 b 182.0 c 81.2 g 23.6 e 37.6 b
Huazhe 2A×F7540-Xa23 115.7 b 16.7 e 25.9 d 285.9 d 222.8 e 77.9 e 23.6 e 53.6 e
Huazhe 2A×F7540-Xa7-Xa21 122.0 d 16.3 d 27.0 f 303.4 e 214.3 d 70.6 b 21.0 a 45.0 d
Huazhe 2A×F7540-Xa7-Xa23 106.0 a 20.3 g 24.0 a 216.6 a 95.0 a 43.8 a 21.6 b 23.2 a
Huazhe 2A×F7540-Xa21-Xa27 123.3 e 19.0 f 32.8 g 383.0 g 293.0 g 76.5 d 23.0 d 53.8 e
Huazhe 2A×F7540 129.0 g 15.7 c 26.5 e 333.0 f 266.1 f 79.9 f 21.8 c 64.1 f
Chunjiang 16A×F7540-Xa7 134.5 g 15.3 f 22.7 e 348.6 e 318.5 g 91.4 e 23.4 a 60.8 g
Chunjiang 16A×F7540-Xa21 125.0 c 10.2 b 21.4 b 268.6 b 252.1 f 93.9 g 24.8 d 34.8 c
Chunjiang 16A×F7540-Xa23 122.3 b 13.3 d 21.4 b 282.5 d 149.0 a 52.8 a 25.8 f 28.7 b
Chunjiang 16A×F7540-Xa7-Xa21 129.0 d 10.5 c 21.1 a 267.7 b 249.4 e 93.1 f 24.2 c 23.0 a
Chunjiang 16A×F7540-Xa7-Xa23 132.7 f 13.7 e 21.8 c 276.5 c 218.7 c 79.1 c 26.2 g 35.6 d
Chunjiang 16A×F7540-Xa21-Xa27 131.0 e 13.7 e 22.4 d 243.4 a 201.2 b 82.7 d 24.0 b 41.1 f
Chunjiang 16A×F7540 117.0 a 9.7 a 21.0 a 366.7 f 236.8 d 64.6 b 25.2 e 38.1 e
Chunjiang 88A×F7540-Xa7 132.3 d 12.3 a 20.8 b 244.0 b 211.6 b 86.7 c 28.8 f 28.8 a
Chunjiang 88A×F7540-Xa21 121.0 a 18.3 g 21.8 d 387.4 f 356.8 g 92.1 d 26.8 c 65.9 g
Chunjiang 88A×F7540-Xa23 125.0 b 21.3 f 28.6 g 315.0 e 290.6 e 92.3 d 30.4 g 60.9 e
Chunjiang 88A×F7540-Xa7-Xa21 140.7 e 13.0 v 22.8 f 312.7 d 292.8 f 93.7 e 25.0 b 47.2 d
Chunjiang 88A×F7540-Xa7-Xa23 129.3 c 18.0 e 19.7 a 234.1 a 216.9 c 92.6 d 28.4 e 45.3 c
Chunjiang 88A×F7540-Xa21-Xa27 121.7 a 14.0 d 22.3 e 258.5 c 176.1 a 68.1 b 24.4 a 32.1 b
Chunjiang 88A×F7540 125.0 b 14.3 c 21.6 c 386.3 f 235.8 d 61.0 a 27.8 d 61.7 f
Huazhong 2A×F7540-Xa7 151.3 g 19.3 f 21.6 a 386.6 f 236.1 c 61.1 b 28.4 e 70.7 g
Huazhong 2A×F7540-Xa21 125.3 c 9.0 a 26.4 d 299.1 b 195.0 a 65.2 d 28.8 f 28.3 a
Huazhong 2A×F7540-Xa23 123.3 b 14.3 d 25.1 c 246.5 a 195.7 a 79.4 e 26.6 b 39.8 c
Huazhong 2A×F7540-Xa7-Xa21 145.3 f 9.0 a 28.2 g 337.5 d 208.3 b 61.7 c 27.4 d 37.6 b
Huazhong 2A×F7540-Xa7-Xa23 115.0 a 10.2 b 22.9 b 343.7 e 302.9 f 88.1 f 23.2 a 62.7 e
Huazhong 2A×F7540-Xa21-Xa27 140.3 e 16.3 e 27.2 f 312.7 c 278.9 e 89.2 g 27.2 c 67.4 f
Huazhong 2A×F7540 138.0 d 13.7 c 26.9 e 477.4 g 262.2 d 54.9 a 28.8 f 53.8 d
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