花生机械脱壳损伤相关农艺指标筛选与QTL定位

doi:10.3724/SP.J.1006.2026.55045

作物学报 ›› 2026, Vol. 52 ›› Issue (2): 644-652.doi: 10.3724/SP.J.1006.2026.55045

• 研究简报 • 上一篇

花生机械脱壳损伤相关农艺指标筛选与QTL定位

张胜忠¹,李国卫¹,戈立江²,王菲菲¹,胡晓辉¹,苗华荣^1,*,李燕³,钟文⁴,陈静^1,*

1 山东省农业科学院, 山东济南250100; 2 山东职业学院, 山东济南250104; 3 招远市农业技术推广中心, 山东烟台265400; 4 山东省种子管理总站, 山东济南250100

收稿日期:2025-07-16 修回日期:2025-10-30 接受日期:2025-10-30 出版日期:2026-02-12 网络出版日期:2025-11-07
通讯作者: 苗华荣, E-mail:1949813628@qq.com; 陈静, E-mail: mianbaohua2008@126.com
基金资助:
本研究由国家重点研发计划项目(2023YFD1202800), 山东省自然科学基金项目(ZR2022MC045)和山东省农业科学院重大科技成果培育工程项目(CXGC2025E02)资助。

Screening and QTL mapping for mechanical shelling damage related traits in peanut

Zhang Sheng-Zhong¹,Li Guo-Wei¹,Ge Li-Jiang²,Wang Fei-Fei¹,Hu Xiao-Hui¹,Miao Hua-Rong^1,*,Li Yan³,Zhong Wen⁴,Chen Jing^1,*

1 Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China; 2 Shandong Polytechnic, Jinan 250104, Shandong, China; 3 Zhaoyuan Agricultural Technology Extension Service Center, Yantai 265400, Shandong, China; 4 Shandong Provincial Seed Management Station, Jinan 250100, Shandong, China

Received:2025-07-16 Revised:2025-10-30 Accepted:2025-10-30 Published:2026-02-12 Published online:2025-11-07
Supported by:
This study was supported by the National Key Research and Development Program of China (2023YFD1202800), Natural Science Foundation of Shandong Province (ZR2022MC045), and Major Scientific and Technological Achievements Cultivation Program of Shandong Academy of Agricultural Sciences (CXGC2025E02).

摘要/Abstract

摘要： 为筛选影响花生机械脱壳质量的农艺性状和探索其遗传基础，本研究利用63个高世代品系，选取3个荚果或籽仁相关性状(出仁率、荚果破损压力和籽仁破损压力)，分别与籽仁损伤率性状进行相关性分析。结果表明，仅籽仁破损压力与籽仁损伤率呈现显著相关。此外，从上述高世代品系中筛选到2个籽仁损伤率低于2.50%的品系材料D9和E10。进一步针对籽仁破损压力性状，以品种花育36号和6-13配组衍生的包含181个家系的重组自交系(recombinant inbred line, RIL)群体为材料，采集了该RIL群体2020—2023年在山东烟台、威海、东营和青岛4个环境下的表型数据。结果表明，籽仁破损压力在RIL群体中均表现为连续分布和超亲遗传，广义遗传率为0.88。利用前期构建的高密度遗传图谱，共定位到8个与籽仁破损压力相关加性QTL，表型贡献率范围为6.04%~28.30%，其中2个主效位点qKCF7和qKCF16.1可在不同环境下表达，其增效等位基因均来自花育36号。共定位到12对相关上位性QTL，共涉及24个SNP标记区间，表型贡献率范围为1.55%~4.01%。本研究结果为后续花生适宜机械脱壳相关性状遗传改良提供了重要靶点及材料支持。

关键词: 花生, 机械脱壳, 籽仁破损压力, 数量性状位点, 定位

Abstract:

To further identify agronomic traits influencing mechanical shelling quality in peanut and explore their genetic basis, this study evaluated 63 advanced-generation lines and focused on three pod- or kernel-related traits: shelling percentage, pod crushing force, and kernel crushing force. Correlation analysis revealed that only kernel crushing force was significantly associated with the damaged kernel rate. Two lines (D9 and E10) with low damaged kernel rates (< 2.50%) were identified. Additionally, a recombinant inbred line (RIL) population of 181 individuals derived from a cross between cultivars Huayu36 and 6-13 was used for QTL mapping of the kernel crushing force trait. Phenotypic data were collected from 2020 to 2023 across four locations: Yantai, Weihai, Dongying, and Qingdao. The trait exhibited continuous variation and transgressive segregation in the RIL population, with a broad-sense heritability of 0.88. Using a previously published high-density genetic map, eight additive QTLs associated with kernel crushing force were identified, explaining 6.04% to 28.30% of the phenotypic variation. Among them, two major QTLs, qKCF7 and qKCF16.1, were stably expressed across multiple environments, with favorable alleles derived from Huayu36. In addition, 12 pairs of epistatic QTLs involving 24 SNP intervals were detected, explaining 1.55% to 4.01% of the phenotypic variation. These findings provide valuable genetic targets and germplasm resources for the future genetic improvement of traits related to mechanical shelling quality in peanut.

Key words: peanut, mechanical shelling, kernel crushing force, quantitative trait locus, mapping

张胜忠, 李国卫, 戈立江, 王菲菲, 胡晓辉, 苗华荣, 李燕, 钟文, 陈静. 花生机械脱壳损伤相关农艺指标筛选与QTL定位[J]. 作物学报, 2026, 52(2): 644-652.

Zhang Sheng-Zhong, Li Guo-Wei, Ge Li-Jiang, Wang Fei-Fei, Hu Xiao-Hui, Miao Hua-Rong, Li Yan, Zhong Wen, Chen Jing. Screening and QTL mapping for mechanical shelling damage related traits in peanut[J]. Acta Agronomica Sinica, 2026, 52(2): 644-652.

参考文献

[1] FAOSTAT. Statistics Database. Rome Available at: http://www.fao.org/statistics/databases/en/.

[2] 禹山林. 中国花生品种及其系谱. 上海: 上海科学技术出版社, 2008.

Yu S L. Peanut Varieties and Their Pedigrees in China. Shanghai: Shanghai Scientific & Technical Publishers, 2008 (in Chinese).

[3] 陈志德, 沈一, 刘永惠, 等. 不同花生品种机械脱壳特性研究. 江苏农业科学, 2023, 51(12): 91–95.

Chen Z D, Shen Y, Liu Y H, et al. Study on mechanical hulling characteristics of different peanut varieties. Jiangsu Agric Sci, 2023, 51(12): 91–95 (in Chinese).

[4] 丁彬, 谢吉先, 冯梦诗, 等. 不同花生荚果类型对机械剥壳效果的影响. 江苏农业科学, 2022, 50(5): 180–184.

Ding B, Xie J X, Feng M S, et al. Effect of different pod types on mechanical husking of peanut. Jiangsu Agric Sci, 2022, 50(5): 180–184 (in Chinese).

[5] Chang A S, Sreedharan A, Schneider K R. Peanut and peanut products: a food safety perspective. Food Control, 2013, 32: 296–303.

[6] 姜慧芳, 任小平, 王圣玉, 等. 花生黄曲霉侵染抗性持久性及种皮完整性对产毒的影响. 作物学报, 2006, 32: 851–855.

Jiang H F, Ren X P, Wang S Y, et al. Durability of resistance to Aspergillus flavus infection and effect of intact testa without injury on aflatoxin production in peanut. Acta Agron Sin, 2006, 32: 851–855 (in Chinese with English abstract).

[7] 王移收. 我国花生产品加工业现状、问题及发展趋势. 中国油料作物学报, 2006, 28: 498–502.

Wang Y S. Present situation, problem and developing trend of peanut food industry in China. Chin J Oil Crop Sci, 2006, 28: 498–502 (in Chinese).

[8] 陆荣, 高连兴, 刘志侠, 等. 中国花生脱壳机技术发展现状与展望. 沈阳农业大学学报, 2020, 51: 124–133.

Lu R, Gao L X, Liu Z X, et al. Development and prospect of technology on peanut sheller of China. J Shenyang Agric Univ, 2020, 51: 124–133 (in Chinese with English abstract).

[9] 郭陞垚, 陈剑洪, 陈永水, 等. 不同剥壳方式、含水率对春花生种子发芽和出苗的影响. 福建农业科技, 2015, 46(5): 1–3.

Guo S Y, Chen J H, Chen Y S, et al. Effects of different hesking ways on seed germination and seedling emergence of spring peanut with different moisture content. Fujian Agric Sci Technol, 2015, 46(5): 1–3 (in Chinese with English abstract).

[10] 王通, 禹山林, 谢宏峰, 等. 花生种皮变化对种子抗破损力的影响. 花生学报, 2020, 49(4): 69–72.

Wang T, Yu S L, Xie H F, et al. Influence of peanut testa variation on resistance of seed to breakage. J Peanut Sci, 2020, 49(4): 69–72 (in Chinese with English abstract).

[11] 王京, 高连兴, 刘志侠, 等. 花生荚果力学特性研究. 农机化研究, 2017, 39(1): 182–186.

Wang J, Gao L X, Liu Z X, et al. Experimental study on peanut pods mechanical property. J Agric Mech Res, 2017, 39(1): 182–186 (in Chinese with English abstract).

[12] 王京, 高连兴, 刘志侠, 等. 典型品种花生米静压力学特性及有限元分析. 沈阳农业大学学报, 2016, 47: 307–313.

Wang J, Gao L X, Liu Z X, et al. Static mechanical property and finite element analysis of typical of peanut varieties. J Shenyang Agric Univ, 2016, 47: 307–313 (in Chinese with English abstract).

[13] 蔡高委, 丛锦玲, 齐贝贝. 花生种子三轴方向起始破碎力. 江苏农业学报, 2019, 35: 716–721.

Cai G W, Cong J L, Qi B B. Experimental study on initial crushing force of peanut seeds in triaxial direction. Jiangsu J Agric Sci, 2019, 35: 716–721 (in Chinese with English abstract).

[14] 许静, 潘丽娟, 陈娜, 等. 不同花生荚果力学特性研究及优异品系筛选. 中国油料作物学报. 2021, 43: 803–815.

Xu J, Pan L J, Chen N, et al. Pods mechanical property of different peanuts and identification of elite varieties (lines). Chin J Oil Crop Sci, 2021, 43: 803–815 (in Chinese with English abstract).

[15] Chen Y N, Ren X P, Zheng Y L, et al. Genetic mapping of yield traits using RIL population derived from Fuchuan Dahuasheng and ICG6375 of peanut (Arachis hypogaea L.). Mol Breed, 2017, 37: 17.

[16] Wang Z H, Huai D X, Zhang Z H, et al. Development of a high-density genetic map based on specific length amplified fragment sequencing and its application in quantitative trait loci analysis for yield-related traits in cultivated peanut. Front Plant Sci, 2018, 9: 827.

[17] Zhang S Z, Hu X H, Wang F F, et al. A stable and major QTL region on chromosome 2 conditions pod shape in cultivated peanut (Arachis hyopgaea L.). J Integr Agric, 2023, 22: 2323–2334.

[18] Zhang S Z, Hu X H, Miao H R, et al. QTL identification for seed weight and size based on a high-density SLAF-seq genetic map in peanut (Arachis hypogaea L.). BMC Plant Biol, 2019, 19: 537.

[19] 刘志侠, 吴国振, 于永强, 等. 花生荚果生物力学特性研究. 沈阳农业大学学报, 2023, 54: 732–740.

Liu Z X, Wu G Z, Yu Y Q, et al. Study on biomechanical characteristics of peanut pod. J Shenyang Agric Univ, 2023, 54: 732–740 (in Chinese with English abstract).

[20] Meng L, Li H H, Zhang L Y, et al. QTL IciMapping: Integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations. Crop J, 2015, 3: 269–283.

[21] Yang J, Guo Z H, Luo L X, et al. Identification of QTL and candidate genes involved in early seedling growth in rice via high-density genetic mapping and RNA-seq. Crop J, 2021, 9: 360–371.

[22] Voorrips R E. MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered, 2002, 93: 77–78.

[23] 迟晓元, 毕竞男, 赵健鑫, 等. 花生荚果力学特性评鉴及早熟种质筛选. 作物学报, 2025, 51: 943–957.

Chi X Y, Bi J N, Zhao J X, et al. Evaluation of mechanical properties of peanut pods and screening of early maturing germplasm. Acta Agron Sin, 2025, 51: 943–957 (in Chinese with English abstract).

[24] Israel G B, Kunta S, Mlelwa W, et al. Genetic characterization and mapping of the shell-strength trait in peanut. BMC Plant Biol, 2024, 24: 1047.

[25] Balakrishnan D, Surapaneni M, Yadavalli V R, et al. Detecting CSSLs and yield QTLs with additive, epistatic and QTL × environment interaction effects from Oryza sativa × O. nivara IRGC81832 cross. Sci Rep, 2020, 10: 7766.

[26] Li Z K, Luo L J, Mei H W, et al. Overdominant epistatic loci are the primary genetic basis of inbreeding depression and heterosis in rice: I. Biomass and grain yield. Genetics, 2001, 158: 1737–1753.

[27] Carlborg Ö, Haley C S. Epistasis: too often neglected in complex trait studies? Nat Rev Genet, 2004, 5: 618–625.

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花生机械脱壳损伤相关农艺指标筛选与QTL定位

Screening and QTL mapping for mechanical shelling damage related traits in peanut

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