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作物学报 ›› 2008, Vol. 34 ›› Issue (09): 1518-1528.doi: 10.3724/SP.J.1006.2008.01518

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

国外栽培豌豆遗传多样性分析及核心种质构建

宗绪晓1,2;关建平2;王述民2;刘庆昌1,*;Robert R Redden3;Rebecca Ford4   

  1. 1中国农业大学农学与生物技术学院, 北京100094; 2中国农业科学院作物科学研究所 / 国家农作物基因资源与基因改良重大科学工程, 北京100081; 3 Australian Temperate Field Crops Collection, Grains Innovation Park, The Department of Primary Industries, Private Bag 260, Horsham, Victoria, Australia 3401; 4 BioMarka, Faculty of Land and Food Resources, The University of Melbourne, Victoria, Australia 3010
  • 收稿日期:2008-01-17 修回日期:1900-01-01 出版日期:2008-09-12 网络出版日期:2008-09-12
  • 通讯作者: 刘庆昌

Genetic Diversity and Core Collection of Alien Pisum sativum L. Germ-plasm

ZONG Xu-Xiao12, GUAN Jian-Ping2,WANG Shu-Min2,LIU Qing-Chang1*,Robert R Redden3,Rebecca Ford4   

  1. 1 Department of Plant Genetics and Breeding, College of Agriculture and Biotechnology, China Agricultural University, Beijing 100094; 2 Institute of Crop Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Australian Temperate Field Crops Collection, Grains Innovation Park, The Department of Primary Industries, Private Bag 260, Hor-sham, Victoria, Australia 3401 BioMarka; 4 Faculty of Land and Food Resources, Melbourne University, Victoria, Australia 3010
  • Received:2008-01-17 Revised:1900-01-01 Published:2008-09-12 Published online:2008-09-12
  • Contact: LIU Qing-Chang

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被引次数

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摘要： 从111对备选SSR引物中筛选出能扩增出清晰稳定单一带的多态性引物21对及其最佳退火温度, 并优化了豌豆SSR标记实验体系。利用上述引物, 对来自于67个国家的731份豌豆栽培种质(Pisum sativum L.)进行遗传多样性分析与核心种质构建。共扩增出109条多态性带, 每对引物平均扩增出5.19个等位变异。SSR等位变异在各大洲间分布不均匀, 有效等位变异数、Shannon’s信息指数(I)洲际间差异明显。各大洲资源群间遗传多样性差异显著, 其中亚洲最高(I = 1.1753), 欧洲其次(I = 1.1387), 俄罗斯联邦(I = 1.0285)、美洲(I = 1.0196)、非洲(I = 0.9254)、大洋洲(I = 0.8608)依次降低。利用Popgene 1.32软件, 依豌豆栽培资源洲际间Nei78遗传距离可聚类成2个组群和4个亚组群; 基于Structure 2.2软件分析, 国外栽培豌豆资源实际由3大类群组成, 并与Popgene 1.32聚类结果呼应得较好。上述两种分析方法均表明, 国外栽培豌豆类群的遗传多样性与其地理分布相关。设计并实践了一套基于Structure分析的科学可靠、逻辑性强的核心种质构建标准化方案, 并依此构建了一套以6.57%的资源(48份)涵盖总体84.4%等位变异的国外栽培豌豆核心种质。

关键词: 豌豆栽培种(Pisum sativum L.), SSR, 遗传多样性, 核心种质, 国外资源

Abstract: China is the second producer of pea in the world with a collection of 5 000 accessions, around 20% of which were introduced from 70 foreign countries. It is essential to assess the genetic diversity among alien pea (Pisum sativum L.) germplasm accessions, to analyze their genetic relationships among various groups of gene pool from different geographical/ecological areas, and to constitute core collection of alien genetic resources of pea. The information generated from the study will be very helpful for the exploration and utilization of overseas pea genetic resources. For the purpose above, 21 SSR primer pairs with unambiguous unique polymorphic bands and their optimum annealing temperatures were successfully screened out from the 111 candidates. Pea’s SSR marker experimental system was optimized thereafter, and adopted for genetic diversity analysis and for constitution of a core collection among the 731 pea accessions from 67 countries of various continents. Using 21 SSR primer pairs, 109 poly-morphic bands were amplified, and 5.19 alleles were detected by each SSR primer pairs on an average. SSR alleles were not uni-formly distributed among continents, and the number of effective alleles and Shannon’s information index (I) were much varied among continental based groups of genetic resources. Significant difference appeared in the pairwise comparisons for genetic diversity between continental based groups of genetic resources. Asia group had the highest level of genetic diversity (I = 1.1753), followed by Europe (I = 1.1387), USSR (I = 1.0285), America (I = 1.0196), Africa (I = 0.9254), and Oceania (I = 0.8608) groups. Two large cluster groups and four cluster sub-groups were identified based on the dendrogram of pairwise Nei78 genetic distance. The clustering results of alien genetic resources revealed geographically broad correlation to their genetic diversity. Three types of population structure within 731 alien pea accessions were inferred by Structure analysis, which also broadly correlated to their geographic origins. A scientific and logical standard methodology for construction of core collection based on Structure analysis was designed and applied in this study, a subset (6.57% of the whole collection) of 48 accessions representing 84.4% of the whole allelic variability was proposed to be a core collection.

Key words: Pisum sativum L., SSR, Genetic diversity, Core collection, Alien genetic resources

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