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作物学报 ›› 2009, Vol. 35 ›› Issue (2): 211-218.doi: 10.3724/SP.J.1006.2009.00211

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

黑龙江部分大豆品种分子ID的构建

高运来1;朱荣胜4**;刘春燕1,2;李文福1;蒋洪蔚1;李灿东1;姚丙晨1;胡国华2,3,*;陈庆山1,*   

  1. 1东北农业大学农学院,黑龙江哈尔滨150030;2黑龙江省农垦科研育种中心,黑龙江哈尔滨150090;3国家大豆工程技术研究中心,黑龙江哈尔滨150050;4东北农业大学理学院,黑龙江哈尔滨150030
  • 收稿日期:2008-03-15 修回日期:2008-09-05 出版日期:2009-02-12 网络出版日期:2008-12-10
  • 通讯作者: 胡国华
  • 基金资助:

    本研究由引进国际先进农业科学技术计划(948计划)项目[2006-G1(A)],国家高技术研究发展计划(863计划)项目(2006AA10Z1F4),黑龙江省博士后科研启动基金项目(LHK-04014)资助.

Establishment of Molecular ID in Soybean Varieties in Heilongjiang

GAO Yun-Lai1,ZHU Rong-Sheng4,**,LIU Chun-Yan1,2,LI Wen-Fu1,JIANG Hong-Wei1,LI Can-Dong1,YAO Bing-Chen1,HU Guo-Hua2,3,*,CHEN Qing-Shan1,*   

  1. 1College of agriculture, Northeast Agricultural University, Harbin 150030,China;2The Crop Research and Breeding Center of Land-Reclamation,Harbin 150090, China; 3The National Research Center of Soybean Engineering and technology, Harbin 150050,China;4College of Science, Northeast Agricural University, harbin 150030, Heilongjiang, China
  • Received:2008-03-15 Revised:2008-09-05 Published:2009-02-12 Published online:2008-12-10
  • Contact: HU Guo-Hua

PDF

3325

被引次数

76

摘要：

以黑龙江13个育种单位6个积温带的83份大豆品种为材料, 选择分布在大豆基因组19个连锁群的43对SSR引物进行检测, 共检测出等位变异157个, 每个引物检测到的等位变异数变化范围为2~7个, 平均为3.65个。将聚丙烯酰胺凝胶电泳得到的谱带统计结果根据等位变异的片段大小数字化, 用自行编制的ID Analysis 1.0软件进行数据分析。结果表明, 仅需9对引物(Satt100、Sat_218、Satt514、Satt551、Satt380、Satt193、Satt191、Satt442、Sat_084)可将83份参试大豆品种完全区分开。构建了一套黑龙江省大豆品种的分子ID。

关键词: 大豆, SSR, 分子身份证

Abstract:

A total of 1 300 cultivars have been bred in China from 1923 to 2005. To well evaluate and use the cultivars, it is necessary to identify scientifically and accurately them by DNA molecular markers due to traditional identification methods can no longer meet requirements. In the paper, 83 cultivars developed by 13 breeding units from six temperature summation zone in Heilongjiang were investigated, 157 polymorphic alleles were detected with 43 SSR primers covering 19 linkage groups of soybean genetic map, for each primer, 2 to 7 allele variations were detected in all cultivars, and 3.65 on average. According to fragment size of allele variation, the data numeralized from the PAGE bands were analyzed by the software ID Analysis 1.0. Nine primers (Satt100, Sat_218, Satt514, Satt551, Satt380, Satt193, Satt191, Satt442, and Sat_084) could be used to identify all 83 soybean cultivars. The PAGE pattern of each cultivar amplified with the 9 primers could be used as a molecular ID of the cultivars. A set of molecular ID were established for 83 soybean cultivars in Heilongjiang.

Key words: Soybean, SSR, Molecular ID

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