欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2009, Vol. 35 ›› Issue (2): 211-218.doi: 10.3724/SP.J.1006.2009.00211

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

黑龙江部分大豆品种分子ID的构建

高运来1;朱荣胜4**;刘春燕1,2;李文福1;蒋洪蔚1;李灿东1;姚丙晨1;胡国华2,3,*;陈庆山1,*   

  1. 1东北农业大学农学院,黑龙江哈尔滨150030;2黑龙江省农垦科研育种中心,黑龙江哈尔滨150090;3国家大豆工程技术研究中心,黑龙江哈尔滨150050;4东北农业大学理学院,黑龙江哈尔滨150030
  • 收稿日期:2008-03-15 修回日期:2008-09-05 出版日期:2009-02-12 网络出版日期:2008-12-10
  • 通讯作者: 胡国华
  • 基金资助:

    本研究由引进国际先进农业科学技术计划(948计划)项目[2006-G1(A)],国家高技术研究发展计划(863计划)项目(2006AA10Z1F4),黑龙江省博士后科研启动基金项目(LHK-04014)资助.

Establishment of Molecular ID in Soybean Varieties in Heilongjiang

GAO Yun-Lai1,ZHU Rong-Sheng4,**,LIU Chun-Yan1,2,LI Wen-Fu1,JIANG Hong-Wei1,LI Can-Dong1,YAO Bing-Chen1,HU Guo-Hua2,3,*,CHEN Qing-Shan1,*   

  1. 1College of agriculture, Northeast Agricultural University, Harbin 150030,China;2The Crop Research and Breeding Center of Land-Reclamation,Harbin 150090, China; 3The National Research Center of Soybean Engineering and technology, Harbin 150050,China;4College of Science, Northeast Agricural University, harbin 150030, Heilongjiang, China
  • Received:2008-03-15 Revised:2008-09-05 Published:2009-02-12 Published online:2008-12-10
  • Contact: HU Guo-Hua

PDF

2753

被引次数

76

摘要：

以黑龙江13个育种单位6个积温带的83份大豆品种为材料, 选择分布在大豆基因组19个连锁群的43对SSR引物进行检测, 共检测出等位变异157个, 每个引物检测到的等位变异数变化范围为2~7个, 平均为3.65个。将聚丙烯酰胺凝胶电泳得到的谱带统计结果根据等位变异的片段大小数字化, 用自行编制的ID Analysis 1.0软件进行数据分析。结果表明, 仅需9对引物(Satt100、Sat_218、Satt514、Satt551、Satt380、Satt193、Satt191、Satt442、Sat_084)可将83份参试大豆品种完全区分开。构建了一套黑龙江省大豆品种的分子ID。

关键词: 大豆, SSR, 分子身份证

Abstract:

A total of 1 300 cultivars have been bred in China from 1923 to 2005. To well evaluate and use the cultivars, it is necessary to identify scientifically and accurately them by DNA molecular markers due to traditional identification methods can no longer meet requirements. In the paper, 83 cultivars developed by 13 breeding units from six temperature summation zone in Heilongjiang were investigated, 157 polymorphic alleles were detected with 43 SSR primers covering 19 linkage groups of soybean genetic map, for each primer, 2 to 7 allele variations were detected in all cultivars, and 3.65 on average. According to fragment size of allele variation, the data numeralized from the PAGE bands were analyzed by the software ID Analysis 1.0. Nine primers (Satt100, Sat_218, Satt514, Satt551, Satt380, Satt193, Satt191, Satt442, and Sat_084) could be used to identify all 83 soybean cultivars. The PAGE pattern of each cultivar amplified with the 9 primers could be used as a molecular ID of the cultivars. A set of molecular ID were established for 83 soybean cultivars in Heilongjiang.

Key words: Soybean, SSR, Molecular ID

[1] Xiong D-J(熊冬金), Zhao T-J(赵团结), Gai J-Y(盖钧镒). Geo-graphical sources of germplasm and their nuclear and cytoplas-mic contribution to soybean cultivars released during 1923 to 2005 in China. Acta Agron Sin (作物学报), 2008, 34(2): 175-183(in Chinese with English abstract)
[2] She H-D(佘花娣), Chen J-T(陈景堂), Huang Y-Q(黄亚群), Zhu L-Y(祝丽英), Chi S-M(池书敏). Progress on variety identifica-tion of crop using DNA fingerprinting. J Agric Univ Hebei (河北农业大学学报), 2003, 26(suppl): 28-32(in Chinese with English abstract)
[3] Powell W M, Morgante J J, Doyle J W, Mcnicol S V. Genepool variation in genus Glycine subgenus soja revealed by polymor-phic nuclear and chloroplast microsatellites. Genetics, 1996, 144: 793-803
[4] Russell J R, Fuller J D, Macaulau M, Hatz B G, Jahoor A, Powell W, Waugh R. Direct Comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theor Appl Genet, 1997, 9: 714-722
[5] McGregor C E, Lambert C A, Greyling M M, Louw J H, Warnich L. Aocomparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tu-berosum L.) germplasm. Euphytica, 2000, 113: 135-144
[6] Cui Y-H(崔艳华), Qiu L-J(邱丽娟), Chang R-Z(常汝镇), Lü W-H(吕文河). A study of genetic diversity of Huanghuai summer sowing soybean in China. Sci Agric Sin (中国农业科学), 2004, 37(1): 15-22(in Chinese with English abstract)
[7] Wang B(王彪), Chang R-Z(常汝镇), Tao L(陶莉), Guan R-X(关荣霞), Yan L(阎丽), Zhang M-H(张明恢), Feng Z-F(冯忠孚), Qiu L-J(邱丽娟). Identification of SSR primer numbers for ana-lyzing genetic diversity of Chinese cultivated soybean. Mol Plant Breed (分子植物育种), 2003, 1(1): 82-88 (in Chinese with Eng-lish abstract)
[8] Wang L-X(王丽侠), Li Y-H(李英慧), Li W(李伟), Zhu L(朱莉), Guan Y(关媛), Ning X-C(宁学成), Guan R-X(关荣霞), Liu Z-X(刘章雄), Chang R-Z(常汝镇), Qiu L-J(邱丽娟). Establish-ment of a core collection of Changjiang spring growing soybean. Biodivers Sci (生物多样性), 2004, 122(6): 578-385(in Chinese with English abstract)
[9] Xie H(谢华), Guan R-X(关荣霞), Chang R-Z(常汝镇), Qiu L-J(邱丽娟). Genetic diversity of Chinese summer soybean germplasm revealed by SSR markers. Chin Sci Bull (科学通报), 2005, 50(6): 526-535(in Chinese)
[10] Song Q J, Merek L F, Shoemaker R C, Lark K G, Concibido V C, Delannay X, Specht J E, Cregan P B. A new integrated genetic linkage map of the soybean. Theor Appl Genet, 2004, 109: 122-128
[11] Xin J-S(辛景树), Guo J-L(郭景伦), Zhang R-B(张软斌). Com-pare and analysis of a determination report in the variety authen-ticity and the purity of seeds by molecular maker. Seeds (种子), 2005, 24(1): 58-60(in Chinese)
[12] Wang L-X(王立新), Li Y-F(李云伏), Chang L-F(常利芳), Huang L(黄岚), Li H-B(李宏博), Ge L-L(葛玲玲), Liu L-H(刘丽华), Yao J(姚骥), Zhao C-P(赵昌平). Method of ID constitution for wheat cultivars. Acta Agron Sin (作物学报), 2007, 33(10): 1738-1740(in Chinese with English abstract)
[13] Lei T-G(雷天刚), Zhang X-K(张学昆), Lu H(陆合), Li J-N(李加纳), Xu X-F(徐新福), Fan Q-X(范其新), Chen L(谌利). SSR finger printing of yellow seeded rapeseed varieties. J Southwest Agric Univ (西南农业大学学报), 2005, 27(3): 361-364(in Chi-nese with English abstract)
[14] Chen B-Y(陈碧云), Zhang X-D(张冬晓), Wu X-M(伍晓明), Liu F-L(刘凤兰), Lu G-Y(陆光远), Gao G-Z(高桂珍). AFLP analy-sis of 89 cultivars attending the national regional varieties trial. Chin J Oil Crop Sci (中国油料作物学报), 2007, 29(2): 9-14(in Chinese with English abstract)
[15] Zhao H-K(赵洪锟), Li Q-Y(李启云), Wang Y-M(王玉民), Zhuang B-C(庄炳昌). Use of RAPD for identification of soybean cultivars. J Jilin Agric Sci (吉林农业科学), 1998, (4): 6-8(in Chinese with English abstract)
[16] Zhao H-K(赵洪锟), Li Q-Y(李启云), Wang Y-M(王玉民), Zhang M(张明), Zhuang B-C(庄炳昌). DNA fingerprint of the main parents and current varieties of soybean in Jilin province. Chin J Oil Crop Sci (中国油料作物学报), 2000, 22(4): 12-16(in Chi-nese with English abstract)
[17] Shi F(史锋), Zhang Y-Z(张耀洲), Zeng G-W(曾广文). Study on identification of soybean cultivars using RAPD markers. Acta Agric Zhejiangensis (浙江农业学报), 1998, 10(4): 16-19(in Chinese with English abstract)
[18] Chen Q-S(陈庆山), Zhang Z-C(张忠臣), Liu C-Y(刘春燕), Xin D-W(辛大伟), Shan D-P(单大鹏), Qiu H-M(邱红梅), Shan C-Y(单彩云). QTL analysis of major agronomic traits in soybean. Sci Agric Sin (中国农业科学), 2007, 40(1): 41-47(in Chinese with English abstract)
[19] Chen Q-S(陈庆山). Comparative analysis of specialty index and genetic similarity on soybean germplasm with resistance to Cer-cospora sojina Hara. China Biotechnol (中国生物工程杂志), 2005, 25(suppl): 155-158(in Chinese with English abstract)
[20] Xue J(薛津). New soybean breeding examined in Heilongjiang province in 2003. Soybean Sci (大豆科学), 2003, 22(2): 154-159(in Chinese)
[21] Russell J, Fuller J, Young G, Thomas B, Taramino G, Macaulay M, Waugh R, Powell W. Discrimination between barley genotypes using microsatelite markers. Genome, 1997, 40: 442-450
[22] Akagi H, Yokozeki Y, Inagaki A, Fujimura T. Highly polymor-phic microsatellites of rice consist of AT repeats and a classifica-tion of closely related cultivars with these microsatellite loci. Theor Appl Genet, 1997, 94: 61-67
[23] Liu B-Y(刘本英), Cheng H(成浩). Molecular fingerprinting technology and its application in tea germplasm resources. J Tea (茶叶), 2007, 33(4): 198-202(in Chinese with English abstract)
[24] Guo W-Z(郭旺珍), Zhang T-Z(张天真), Pan J-J(潘家驹), He J-L(何金龙). Analysis of RAPD finger printing on main cotton cultivars in China. J Agric Biotechnol (农业生物技术学报), 1996, 4(2): 129-134(in Chinese with English abstract
[1] 陈玲玲, 李战, 刘亭萱, 谷勇哲, 宋健, 王俊, 邱丽娟. 基于783份大豆种质资源的叶柄夹角全基因组关联分析[J]. 作物学报, 2022, 48(6): 1333-1345.
[2] 杨欢, 周颖, 陈平, 杜青, 郑本川, 蒲甜, 温晶, 杨文钰, 雍太文. 玉米-豆科作物带状间套作对养分吸收利用及产量优势的影响[J]. 作物学报, 2022, 48(6): 1476-1487.
[3] 王炫栋, 杨孙玉悦, 高润杰, 余俊杰, 郑丹沛, 倪峰, 蒋冬花. 拮抗大豆斑疹病菌放线菌菌株的筛选和促生作用及防效研究[J]. 作物学报, 2022, 48(6): 1546-1557.
[4] 于春淼, 张勇, 王好让, 杨兴勇, 董全中, 薛红, 张明明, 李微微, 王磊, 胡凯凤, 谷勇哲, 邱丽娟. 栽培大豆×半野生大豆高密度遗传图谱构建及株高QTL定位[J]. 作物学报, 2022, 48(5): 1091-1102.
[5] 李阿立, 冯雅楠, 李萍, 张东升, 宗毓铮, 林文, 郝兴宇. 大豆叶片响应CO2浓度升高、干旱及其交互作用的转录组分析[J]. 作物学报, 2022, 48(5): 1103-1118.
[6] 彭西红, 陈平, 杜青, 杨雪丽, 任俊波, 郑本川, 罗凯, 谢琛, 雷鹿, 雍太文, 杨文钰. 减量施氮对带状套作大豆土壤通气环境及结瘤固氮的影响[J]. 作物学报, 2022, 48(5): 1199-1209.
[7] 王好让, 张勇, 于春淼, 董全中, 李微微, 胡凯凤, 张明明, 薛红, 杨梦平, 宋继玲, 王磊, 杨兴勇, 邱丽娟. 大豆突变体ygl2黄绿叶基因的精细定位[J]. 作物学报, 2022, 48(4): 791-800.
[8] 陈小红, 林元香, 王倩, 丁敏, 王海岗, 陈凌, 高志军, 王瑞云, 乔治军. 基于高基元SSR构建黍稷种质资源的分子身份证[J]. 作物学报, 2022, 48(4): 908-919.
[9] 张霞, 于卓, 金兴红, 于肖夏, 李景伟, 李佳奇. 马铃薯SSR引物的开发、特征分析及在彩色马铃薯材料中的扩增研究[J]. 作物学报, 2022, 48(4): 920-929.
[10] 李瑞东, 尹阳阳, 宋雯雯, 武婷婷, 孙石, 韩天富, 徐彩龙, 吴存祥, 胡水秀. 增密对不同分枝类型大豆品种同化物积累和产量的影响[J]. 作物学报, 2022, 48(4): 942-951.
[11] 杜浩, 程玉汉, 李泰, 侯智红, 黎永力, 南海洋, 董利东, 刘宝辉, 程群. 利用Ln位点进行分子设计提高大豆单荚粒数[J]. 作物学报, 2022, 48(3): 565-571.
[12] 周悦, 赵志华, 张宏宁, 孔佑宾. 大豆紫色酸性磷酸酶基因GmPAP14启动子克隆与功能分析[J]. 作物学报, 2022, 48(3): 590-596.
[13] 王娟, 张彦威, 焦铸锦, 刘盼盼, 常玮. 利用PyBSASeq算法挖掘大豆百粒重相关位点与候选基因[J]. 作物学报, 2022, 48(3): 635-643.
[14] 董衍坤, 黄定全, 高震, 陈栩. 大豆PIN-Like (PILS)基因家族的鉴定、表达分析及在根瘤共生固氮过程中的功能[J]. 作物学报, 2022, 48(2): 353-366.
[15] 张国伟, 李凯, 李思嘉, 王晓婧, 杨长琴, 刘瑞显. 减库对大豆叶片碳代谢的影响[J]. 作物学报, 2022, 48(2): 529-537.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2