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作物学报 ›› 2009, Vol. 35 ›› Issue (2): 262-269.doi: 10.3724/SP.J.1006.2009.00262

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

中国滇蔗茅种质资源遗传多样性的AFLP分析

刘新龙1;蔡青2,*;毕艳1;陆鑫1;马丽1;应雄美1   

  1. 1云南省农业科学院甘蔗研究所，云南开远661600；2云南省农业科学院生物技术与种质资源研究所，云南昆明650223
  • 收稿日期:2008-06-16 修回日期:2008-09-04 出版日期:2009-02-12 网络出版日期:2008-12-11
  • 通讯作者: 蔡青
  • 基金资助:

    本研究由云南省重点基金项目(2006C0013Z)，国家财政部行业专项（nyhyzx07-019），科技部广西省部会商项目（2007BAD30B02）资助

Genetic Diversity Analysis for Germplasm of Erianthus rockii in China

LIU Xin-Long1,CAI Qing2,*,BI Yan1,LU Xin1,MA Li1,YING Xiong-Mei1   

  1. 1Sugarcane Research institute, Yunnan Academy Academy of Agricultural sciences,Kaiyuan 661600,China;2Biotechnology & Geneic Germplasm institute, Yunnan Academy of agricultural sciences, Kunming 650223,China
  • Received:2008-06-16 Revised:2008-09-04 Published:2009-02-12 Published online:2008-12-11
  • Contact: CAI Qing

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1814

被引次数

44

摘要：

利用10对AFLP引物对来自国家甘蔗资源圃的41份滇蔗茅(Erianthus rockii)无性系进行扩增, 获得860个片段, 多态性条带629个, 多态性条带比率0.73, 特异片段54个。遗传相似性系数、UPGMA聚类和主效应分析表明, 在相似系数0.52处做切割线，毛轴野古草、斑茅和滇蔗茅无性系分为3个类群；在相似系数0.715处做切割线时, 又将41份滇蔗茅无性系划分为3个大类群, 云滇07/23独自形成A类群, 鉴于其叶鞘背毛，有待做进一步的分析；B类群3份无性系, 主要来自云南西南部高海拔地区；C类群37份无性系, 其中30份来自云南西南方向的保山、德宏地区, 其他地区7份；在相似系数0.738处做切割时, 将C类群37份无性系划分为4个亚类群, 亚类群的划分反映出明显的地域分布规律, 来自同一地区的无性系多聚为一类；在相似系数0.765处做切割可将C4亚类群划分为4个亚类群(C4-1, C4-2, C4-3, C4-4), 其中C4-3亚类群中云滇07/9/1与云滇99/4分子聚类最为相似, 可作为复份材料保存；C4-3亚类群在相似系数0.773处切割又可以分为3个分支类群, 以上分析反映出同一地区无性系之间具有丰富的遗传变异；主效应分析反映的属间、种间、无性系之间的亲缘关系与分子聚类分析结果一致；由此可见, 丰富的地理生态条件造就了滇蔗茅丰富的遗传多样性和明显的地域性分布规律。

关键词: 滇蔗茅, AFLP, 遗传多样性

Abstract:

Erianthus rockii is the most important wild resource in sugarcane germplasm, which mainly distributes in Yunnan Province. To well utilize the germplasm in breeding program, it is necessary to understand its genetic background. In this research, 41 clones from national nursery of sugarcane germ were amplified with 10 informative AFLP primers. In total, 860 bands were detected, 629 of which were polymorphic, 36 of which were specific bands, and rate of polymorphic bands was 0.73. The results of genetic similarity coefficients, UPGMA cluster, and principal component analysis showed that 41 sugarcane clones were divided into three groups at 0.715 of genetic similarity coefficient:Yundian 07/23 was only grouped into group A and should be further identified due to its back hair on the sheath different from others, group B included three sugarcane clones from high altitude area, group C included 37 sugarcane clones, containing of 30 from southwest of Yunnan, and 7 from other region. Groups C was divided into 4 sub-groups at 0.738 of genetic similarity coefficient, and the cluster relation was obviouslyreflected the geographic distribution of the clones.Sub-group C4 was divided into 4 sub-sub-groups at 0.765 of genetic similarity coefficient. Yundian07/9/1 and Yundian99/4 in sub-sub-group C4-3 were the most similar, indicating that two clones were duplicate. Sub-sub-group C4-3 included three branches according to 0.773 of genetic similarity coefficient. Principal component analysis for all clones indicated same result obtained from genetic similarity coefficients analysis. Therefore, complex geographical ecological environment is important factor to the genetic diversity and geographical distribution of E. rockii clones.

Key words: Erianthus rockii, AFLP marker, Genetic diversity

[1] Editorial Board of the Flora of China (中国植物志编辑部委员会). Flora of China, Vol. 9 (中国植物志·第9卷). Beijing: Sci-ence Press, 2002. pp 48-49(in Chinese)
[2] Chen R-K(陈如凯). Theory and Practice in Modern Sugarcane Breeding (现代甘蔗育种的理论与实践). Beijing: China Agri-culture Press, 2003. pp 2-7(in Chinese)
[3] Wang L-P(王丽萍), Cai Q(蔡青), Lu X(陆鑫), Ma L(马丽), Liu X-L(刘新龙), Li W-F(李文凤), Xia H-M(夏红明). Study of wild species Erianthus rockii germplasm innovation and use. Sugar Crops China (中国糖料), 2008, (2): 8-11(in Chinese with Eng-lish abstract)
[4] Xiao F-H(肖凤回). Comparison between E. arundinaceus and some species under Saccharum and Erianthus on the plant mor-phology and isozyme. Sugarcane (甘蔗), 1994, 1(1): 22-27(in Chinese with English abstract)
[5] Cai Q(蔡青), Fan Y-H(范源洪), Aitken K, Piperidis G, Mcintyre C L, Jackson P. Assessment of the phylogenetic relationships within the “Saccharum complex” using AFLP markers. Acta Agron Sin (作物学报), 2005, 31(5): 551-559(in Chinese with English abstract)
[6] Cai Q, Aitken K, Fan Y H, Piperidis G, Jackson P, Mcintyre C L. A preliminary assessment of the genetic relationship between Erian-thus rockii and the ‘‘Saccharum complex’’ using micro-satellite (SSR) and AFLP markers. Plant Sci, 2005, 169: 976-984
[7] Guo X-M(郭雄明), Xue X(薛霞), Chen H(陈华). Progress in re-search on amplified fragment length polymorphism (AFLP). China J Comparative Med (中国比较医学杂志), 2006, 16(6): 369-372(in Chinese with English abstract)
[8] Doyle J J, Doyle J I. Isolation of plant DNA from fresh tissue. Focus, 1990, 12: 149-151
[9] Vos P, Hogers R, Bleeker M, Reijans M, Van Der Lee T, Hornes M. AFLP: A new concept for DNA fingerprinting. Nucl Acids Res, 1995, 23: 4407-4414
[10] Powell W, Morgante M, Andre C. The comparison RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed, 1996, 2: 225-238
[11] Nei M, Kumar S. Molecular Evolution and Phylogenetics. New York: Oxford University Press, 2000. pp 87-88
[12] Xu A-X(徐爱遐), Ma C-Z(马朝芝), Xiao E-S(肖恩时), Quan J-C(权景春), Ma C-Z(马长珍), Tian G-W(田广文), Tu J-X(涂金星), Fu T-D(傅廷栋), Zhang G-S(张改生). Genetic diversity of Brassica juncea from western China. Acta Agron Sin (作物学报), 2008, 34(5): 754-763(in Chinese with English abstract)
[13] Yan L(闫龙), Guan J-P(关建平), Zong X-X(宗绪晓). Genetic diversity analysis of pigeonpea germplasm resources by AFLP. Acta Agron Sin (作物学报), 2007, 33(5): 790-798(in Chinese with English abstract)
[14] Liu Y(刘勇), Sun Z-H(孙中海), Liu D-C(刘德春), Wu B(吴波), Tao J-J(陶建军). Assessment of the genetic diversity of pummelo germplasms using AFLP and SSR markers. Sci Agric Sin (中国农业科学), 2005, 38(11): 2308-2315(in Chinese with English ab-stract)
[15] Zhuang N-S(庄南生), Zheng C-M(郑成木), Huang D-Y(黄东益), Tang Y-Q(唐燕琼), Gao H-Q(高和琼). AFLP analysis for sugar-cane Germplasms. Acta Agron Sin (作物学报), 2005, 31(4): 444-450(in Chinese with English abstract)
[16] Aitken K S, Li J C, Jackson P, Piperidis G, Mcintyre C L. AFLP analysis of genetic diversity within Saccharum officinarum and comparison with sugarcane cultivars. Aust J Agric Res, 2006, 57: 1167-1184
[17] Selvi A, Nair N V, Noyer J L, Singh N K, Balasundaram N, Bansal K C, Koundal K R, Mohapatra T. AFLP analysis of the phenetic organization and genetic diversity in the sugarcane com-plex, Saccharum and Erianthus. Genet Resour Crop Evol, 2006, 53: 831-842
[18] Liu X-L(刘新龙), Cai Q(蔡青), Wang L-P(王丽萍), Ma L(马丽), Lu X(陆鑫), Ying X-M(应雄美), Mao J(毛钧). The advance of sugarcane germplasm resource in genetic and crossing diversity utilization. Sugar Crops China (中国糖料), 2007, (1): 46-49(in Chinese with English abstract)
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