花生栽培种SSR遗传图谱的构建

doi:10.3724/SP.J.1006.2009.00395

摘要/Abstract

摘要：

花生栽培种品种间分子多态性相对缺乏, 至今未构建出较完整的分子遗传图谱。本研究以粤油13和阜95-5为亲本, 通过杂交构建包含184个F6重组自交系的遗传作图群体。采用652对genomic-SSR引物和392对EST-SSR引物对亲本进行多态性检测, 从中筛选出121对多态性引物, 在亲本中共检测到123个多态性位点。利用作图群体对多态性SSR位点进行遗传连锁分析, 获得包含108个SSR标记(102个genomic-SSR标记和6个EST-SSR标记), 涉及20个连锁群, 总长568 cM, 平均图距为6.45 cM的花生栽培种遗传图谱。与前人构建的花生野生种(A. duranensis × A. stenosperma, AA genome)SSR遗传图谱比较, 初步确定本研究构建的遗传图谱中有11个连锁群与野生种遗传图谱的6个连锁群存在同源关系。

关键词: 花生, SSR, 遗传图谱

Abstract:

Molecular genetic map is a fundamental organizational tool for genomic research. However, a comprehensive genetic linkage map for peanut cultivars has not yet been developed due to extremely low levels of DNA polymorphisms in cultivated peanut. In this study, 184 recombinant inbred lines (RIL), derived from a cross between two Spanish type peanut cultivars (Yueyou 13 × Fu95-5), were used as mapping population. A total of 652 pairs of genomic-SSR primers and 392 pairs of EST-SSR primers were used to detect the polymorphisms between the parental cultivars. Of them, 121 SSR primer pairs amplified 123 segregating loci and were selected to analyze the RIL population. A genetic linkage map consisting of 108 SSR loci (102 genomic-SSR and 6 EST-SSR) in 20 linkage groups and covers 568 cM with an average distance of 6.45 cM of intermaker was constructed. By comparing the SSR genetic map of Arachis (A. duranensis × A. stenosperma) with AA genome, 11 linkage groups in the linkage map constructed in this study were confirmed to have homology with 6 linkage groups of wild peanut species.

Key words: Peanut (Arachis hypogaea L.)')">Peanut (Arachis hypogaea L.), SSR, Genetic linkage map

洪彦彬;梁炫强;陈小平;刘海燕;周桂元;李少雄;温世杰. 花生栽培种SSR遗传图谱的构建[J]. 作物学报, 2009, 35(3): 395-402.

HONG Yan-Bin;LIANG Xuan-Qiang;CHEN Xiao-Ping;LIU Hai-Yan;ZHOU Gui-Yuan;LI Shao-Xiong;WEN Shi-Jie. Construction of Genetic Linkage Map in Peanut(Arachis hypogaea L.) Cultivars[J]. Acta Agron Sin, 2009, 35(3): 395-402.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2009.00395

https://zwxb.chinacrops.org/CN/Y2009/V35/I3/395

参考文献

[1]Kochert G, Halward T, Branch W D, Simpson C E. RFLP variability in peanut(Arachis hypogaea L.) cultivars and wild species. Theor Appl Genet, 1991, 81: 565-570
[2]Subramanian V, Gurtu S, Rao R C N, Nigam S N. Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (PAPD) assay. Genome, 2000, 43: 656-660
[3]Milla S R, Isleib T G, Stalker H T. Taxonomic relationships among Arachis sect. Arachis species as revealed by AFLP markers. Genome, 2005, 8: 1-11
[4]Herselman L, Thwaites R, Kimmins F M, Courtois B, van der Merwe P J, Seal S E. Identification and mapping of AFLP markers linked to peanut(Arachis hypogaea L.) resistance to the aphid vector of groundnut rosette disease. Theor Appl Genet, 2004, 109: 1426-1433
[5]Gimenes M A, Hoshino A A, Barbosa A V, Palmieri D A, Lopes C R. Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea). BMC Plant Biol, 2007, 7: 9
[6]Hong Y-B(洪彦彬), Liang X-Q(梁炫强), Chen X-P(陈小平), Lin K-Y(林坤耀), Zhou G-Y(周桂元), Li S-X(李少雄), Liu H-Y(刘海燕). Genetic differences in peanut cultivated types (Arachis hypogaea) revealed by SSR polymorphism. Mol Plant Breed (分子植物育种), 2008, 6(1): 71-78 (in Chinese with English abstract)
[7]Jiang H-F(姜慧芳), Chen B-Y(陈本银), Ren X-P(任小平), Liao B-S(廖伯寿), Lei Y(雷永), Fu T-D(傅廷栋), Ma C-Z(马朝芝), Mace E, Crouch J H. Identification of SSR markers linked to bacterial wilt resistance of peanut with RIL. Chin J Oil Crop Sci (中国油料作物学报), 2007, 29(1): 26-30(in Chinese with English abstract)
[8]Kantety R V, La Rota M, Matthews D E. Data mining for simple sequence repeats in expressed sequence tags from barley, maize, rice, sorghum and wheat. Plant Mol Biol, 2002, 48: 501-510
[9]Lu S-D(卢圣栋). Current Protocols for Molecular Biology (现代分子生物学实验技术). Beijing: Chinese Academy of Medical Sciences & Peking Union Medical College Press, 1999. pp 101-136
[10]Hopkins M S, Casa A M, Wang T, Mitchell S E, Dean R E, Kochert G D, Kresovich S. Discovery and characterization of polymorphic simple sequence repeats (SSR) in peanut. Crop Sci, 1999, 39: 1243-1247
[11]Palmieri D A, Hoshino A A, Bravo J P, Lopes C R, Gimenes M A. Isolation and characterization of microsatellite loci from the forage species Arachis Pintoi (Genus Arachis). Mol Ecol Notes, 2002, 2: 551-553
[12]He G, Meng R, Newman M, Gao G, Pittman R N, Prakash C S. Microsatellites as DNA markers in cultivated peanut (A. hypogaea L.). BMC Plant Biol, 2003, 3: 3
[13]Ferguson M E, Burow M D, Schulze S R, Bramel P J, Paterson A H, Kresovich S, Mitchell S. Microsatellite identification and characterization in peanut(A. hypogaea L.). Theor Appl Genet, 2004, 108: 1064-1070
[14]Moretzsohn M C, Hopkins M S, Mitchell S E, Kresovich S, Valls J F, Ferreira M E. Genetic diversity of peanut (Arachis hypogaea L.) and its wild relatives based on the analysis of hypervariable regions of the genome. BMC Plant Biol, 2004, 4: 11
[15]Moretzsohn M C, Leoi L, Proite K, Guimaras P M, Leal-Bertioli S C M, Gimenes M A, Martins W S, Valls J F M, Grattapaglia D, Bertioli D J. A microsatellite-based, gene-rich linkage map for the AA genome of Arachis (Fabaceae). Theor Appl Genet, 2005, 111: 1060-1071
[16]Palmieri D A, Bechara M D, Curi R A, Gimenes M A, Lopes C R. Novel polymorphic microsatellite markers in section Caulorrhizae (Arachis, Fabaceae). Mol Ecol Notes, 2005, 5: 77-79
[17]Martins W, Sousa D D, Proite K, Guinaraes P, Moretzsohn M, Bertioli D. New softwares for automated microsatellite marker development. Nucl Acids Res, 2006, 34:1-4
[18]Varshney R K, Graner A, Sorrells M E. Genetic microsatellite markers in plants: features and applications. Trends Biotechnol, 2005, 23: 48-55
[19]Burow M D, Simpson C E, Starr J L, Paterson A H. Transmission genetics of chromatin from a synthetic amphidiploids to cultivated peanut (Arachis hypogaea L.): Broadening the gene pool of a monophyletic polyploid species. Genetics, 2001, 159: 823-837
[20]Halward T M, Stalker H T, Kochert G. Development of an RFLP linkage map in diploid peanut species. Theor Appl Genet, 1993, 87: 379-384
[21]Sewell M M, Sherman B K, Neale D B. A consensus map for loblolly (Pinustaeda L.): I. Construction and intergration of individual linkage maps from two outbred three-generation pedigree. Genetics, 1999, 151: 321-330
[22]Butcher P A, Williams E R, Whitaker D, Kiene K L, Temesgen B. Improving linkage analysis in outcrossed forest trees --- an example from Acacia mangium. Theor Appl Genet, 2001, 104: 1185-1191
[23]Gosselin I, Zhou Y, Bousquet J, Isabel N. Megagametophyte-derived linkage maps of white spruce (Picea glauca) based on RAPD, SCAR and ESTP markers. Theor Appl Genet, 2002, 104: 987-997
[24]Stam P. Construction of integrated genetic linkage maps by means of a new computer package JoinMap. Plant J, 1993, 3: 739-744
[25]Bozhko M, Riegel R, Schubert R, Muller-Starck G. A cyclophilin gene marker confirming geographical differentiation of Norway spruce populations and indicating viability response on excess soil-born salinity. Mol Ecol, 2003, 12: 3147-55
[26]Schubert R, Starck G M, Riegel R. Development of EST-PCR markers and monitoring their intrapopulational genetic variation in Picea abies L. Karst. Theor Appl Genet, 2001, 103: 1223-1231
[27]Brown G R, Kadel E E, Bassoni D L, Kiehne K L, Temesgen B, van Buijtenen J P, Sewell M M, Marshall K A, Neale D B. Anchored reference loci in loblolly pine (Pinus taeda L.) for integrating pine genomics. Genetics, 2001, 159: 799-809
[28]Cordeiro G M, Casu R, McIntyre C L, Manners J M, Henry R J. Microsatellite markers from sugarcane (Saccharum spp.) ESTs cross transferable to erianthus and sorghum. Plant Sci, 2001, 160: 1115-1123
[29]Varshney P K, Sigmund R, Borner A, Korzun V, Stein N, Sorrells M E, Langridge P, Grane A. Interspecific transferability and comparative mapping of barley EST-SSR markers in wheat, rye and rice. Plant Sci, 2005, 168: 195-202
[30]Cho Y G, Ishii T, Temnykh S, Chen X, Lipovich L, McCouch S R, Park W D, Ayres N, Cartinhour S. Diversity of microsatellites derived from genomic libraries and GenBank sequences in rice (Oryza sativa L.). Theor Appl Genet, 2000, 100: 713-722
[31]Cordeiro G M, Casu R, McIntyre C L, Manners J M, Henry R J. Microsatellite markers from sugarcane (Saccharum spp.) ESTs cross transferable to erianthus and sorghum. Plant Sci, 2001, 160: 1115-1123
[32]Nicot N, Chiquet V, Gandon B, Amilhat L, Legeai F, Leroy P, Bernard M, Sourdille P. Study of simple sequence repeat(SSR) markers from wheat expressed sequences tags (EST). Theor Appl Genet, 2004, 109: 800-805
[33]Areshchenkova T, Ganal M W. Comparative analysis of polymorphism and chromosomal location of tomato microsatellite markers isolated from different sources. Theor Appl Genet, 2002, 104: 229-235
[34]Cordeiro G M, Casu R, McIntyre C L, Manners J M, Henry R J. Microsatellite markers from sugarcane (Saccharum spp.) ESTs cross transferable to erianthus and sorghum. Plant Sci, 2001, 160: 1115-1123
[35]Ramsay L, Macaulay M, Ivanissivich S, MacLean K, Cardle L, Fuller J, Edwards K, Tuvesson S, Morgante M, Massari A, Maestri E, Marniorlin N, Sjakste T, Ganal M, Powell W, Powell W, Waugh R. A simple sequence repeat-based linkage map of barley. Genetics, 2000, 156: 1997-2005
[36]Kasha K J, Kao K N. High frequency haploid production in barley (Hordeum vulgare L.). Nature, 1970, 225: 874-876
[37]Bradshaw H D, Stettler R F. Molecular genetics of growth and development in Populus: II. Segregation distortion due to genetics load. Theor Appl Genet, 1994, 89: 551-558
[38]Nikaido A, Yoshimaru H, Tsumura Y, Suyama Y, Murai M. Segregation distortion of AFLP markers in Cryptomeria japonica. Genes Genet Syst, 1999, 74: 55-59
[39]Echt C S, Nelson C D. Linkage mapping and genome length in eastern white pine (Pinus strobes L.). Theor Appl Genet, 1997, 94: 1031-1037
[40]Myburg A A, Griffin A R, Sederoff R R, Whetten R W. Comparative genetic linkage maps of Eucalyptus grandis, Eucalyptus globules and their F1 hybrid based on a double pseudo-backcross mapping approach. Theor Appl Genet, 2003, 107: 1028-1042
[41]Singh A K, Moss J P. Utilization of wild relatives in genetic improvement of Arachis hypogaea L: II. Chromosome complements of species of section Arachis. Theor Appl Genet, 1982, 61: 305-314
[42]Wynne J C, Halward T. Cytogenetics and genetics of Arachis. Crit Rev Plant Sci, 1989, 8: 189-220

相关文章 15

[1]	郑玉珍, 齐飞艳, 孙子淇, 刘华, 秦利, 石磊, 王娟, 汪蒙蒙, 韩锁义, 徐静, 苗利娟, 黄冰艳, 董文召, 郑峥, 张新友. 花生籽仁总超长链脂肪酸和7种脂肪酸组分的QTL定位[J]. 作物学报, 2026, 52(6): 1646-1657.
[2]	陆一楚, 李振影, 买春海, 赵晓蕊, 王利祥. 夜间复合体关键基因AhLUX1正向调控花生结瘤的功能研究[J]. 作物学报, 2026, 52(6): 1658-1668.
[3]	谷春苗, 王润风, 黄璐, 刘浩, 鲁清, 李海芬, 李少雄, 何双呈, 洪彦彬, 陈小平, 谭斌, 余倩霞. 花生WOX基因家族的全基因组分析及不定芽再生候选基因的鉴定[J]. 作物学报, 2026, 52(5): 1326-1340.
[4]	张颖星, 宋裕祯, 王跃, 曹越, 曹晓宁, 王瑞云. EMS诱导糜子优异性状突变体的筛选及表型分析[J]. 作物学报, 2026, 52(5): 1388-1400.
[5]	田春艳, 陆鑫, 吴才文, 徐超华, 刘家勇, 边芯, 桃联安. 基于荧光SSR的甘蔗创新种质遗传多样性分析及育种潜力评估[J]. 作物学报, 2026, 52(4): 1057-1072.
[6]	于天一, 王春晓, 肖丽, 钟召迪, 王宣仓, 赵勇, 路亚, 吴月, 吴正锋. 不同结瘤特性花生品种氮素累积、产量及品质特性对氮肥用量的响应[J]. 作物学报, 2026, 52(3): 881-894.
[7]	张胜忠, 李国卫, 戈立江, 王菲菲, 胡晓辉, 苗华荣, 李燕, 钟文, 陈静. 花生机械脱壳损伤相关农艺指标筛选与QTL定位[J]. 作物学报, 2026, 52(2): 644-652.
[8]	王菲菲, 张胜忠, 杨贵华, 苗华荣, 胡晓辉, 张则林, 刘莎莎, 乔利仙, 单世华, 陈静. 331份花生种质苗期耐盐性综合评价和强耐盐种质鉴选[J]. 作物学报, 2026, 52(1): 279-294.
[9]	金欣欣, 宋亚辉, 苏俏, 杨永庆, 王瑾. 高产高油高油酸花生品种的生长发育及干物质生产特征[J]. 作物学报, 2026, 52(1): 191-201.
[10]	迟晓元, 刘庆, 张君, 赵旭红, 李美, 于天一, 潘丽娟, 许静, 姜骁, 殷祥贞, 马俊卿, 陈娜. 不同花生品种(系)耐盐碱性田间鉴定及各性状指标相关性研究[J]. 作物学报, 2026, 52(1): 85-98.
[11]	孙辰硕, 张月, 田泽锴, 晏立英, 康彦平, 陈玉宁, 王欣, 淮东欣, 王前前, 姜慧芳, 罗怀勇, 黄莉, 廖伯寿, 王志慧, 雷永. 花生种质果柄强度的遗传分化与主要影响因子分析[J]. 作物学报, 2026, 52(1): 118-130.
[12]	万书波, 张佳蕾, 高华鑫, 王才斌. 中国花生高产栽培研究进展与展望[J]. 作物学报, 2025, 51(7): 1703-1711.
[13]	郭腾达, 崔梦杰, 陈琳杰, 韩锁义, 郭敬坤, 吴晨迪, 付留洋, 黄冰艳, 董文召, 张新友. 花生磷脂酰肌醇转运蛋白基因AhSFH的克隆及其响应黄曲霉菌侵染的表达特征分析[J]. 作物学报, 2025, 51(6): 1489-1500.
[14]	李文佳, 廖泳俊, 黄璐, 鲁清, 李少雄, 陈小平, 金晶炜, 王润风. 花生开花时间的全基因组关联分析及候选基因筛选[J]. 作物学报, 2025, 51(5): 1400-1408.
[15]	林伟津, 郭泽佳, 刘浩, 李海芬, 王润风, 黄璐, 余倩霞, 陈小平, 洪彦彬, 李少雄, 鲁清. 花生荚果产量相关性状QTL定位与候选基因分析[J]. 作物学报, 2025, 51(4): 969-981.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed