作物学报 ›› 2009, Vol. 35 ›› Issue (3): 403-411.doi: 10.3724/SP.J.1006.2009.00403
任妍12;梁丹2;张平平23;何中虎24;陈静5;傅体华1;夏先春2*
REN Yan12;LIANG Dan2;ZHANG Ping-Ping23;HE Zhong-Hu24;CHEN Jing5;FU Ti-Hua1;XIA Xian-Chun2*
摘要:
高分子量谷蛋白亚基Bx7的超量表达对提高小麦面筋强度有重要作用。利用反相高效液相色谱(RP-HPLC)和STS标记检测了163份中国和CIMMYT小麦品种(系)的高分子谷蛋白亚基Bx7超量表达基因(Bx7OE)。结果表明,TaBAC1215C06-F517/R964标记和TaBAC1215C06-F24671/R25515标记可分别在含有Bx7OE基因的材料中扩增出447 bp和844 bp的特异带,在不含Bx7OE基因的材料中无相应目标带,两个STS标记的检测结果完全一致。在163份小麦品种(系)中,11份品种(系)含有Bx7OE基因,占总数的6.7%。RP-HPLC与STS标记检测结果一致。利用这两个STS标记可以方便、快速、准确地检测Bx7OE基因。
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