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作物学报 ›› 2009, Vol. 35 ›› Issue (3): 403-411.doi: 10.3724/SP.J.1006.2009.00403

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

中国和CIMMYT小麦品种Bx7亚基超量表达基因(Bx7OE)的分子检测

任妍12;梁丹2;张平平23;何中虎24;陈静5;傅体华1;夏先春2*   

  1. 1四川农业大学农学院,四川雅安625014;2中国农业科学院作物科学研究所国家小麦改良中心/国家农作物基因资源与基因改良重大科学工程,北京100081;3江苏省农业科学院农业生物技术研究所,江苏南京210014;4CIMMYT中国办事处,北京100081;5中国科学院成都生物研究所,四川成都610041
  • 收稿日期:2008-06-30 修回日期:2008-09-03 出版日期:2009-03-12 网络出版日期:2009-01-15
  • 通讯作者: 夏先春
  • 基金资助:

    本研究由国家自然科学基金项目（30871527），引进国际先进农业科学技术计划（948计划）重大国际合作项目（2006-G2），国家高技术研究发展计划（863计划）项目（2006AA10Z1A7,2006AA100102),四川省科技厅育种攻关项目资助

Characterization of Overexpressed Bx7 Gene(Bx7OE) in Chinese and CIMMYT Wheats by STS Markers

REN Yan12;LIANG Dan2;ZHANG Ping-Ping23;HE Zhong-Hu24;CHEN Jing5;FU Ti-Hua1;XIA Xian-Chun2*   

  1. 1College of Agronomy,Sichuan Agricultural University,Ya'an 625014,China;2National Wheat Improvement Center,Institute of Crop Sciences/National key Facility for Crop Gene Resource and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081,China; 3 Institute of Agricultural Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014,China;4CIMMYT China Office, Beijing 100081,China;5Institute of Biology,Chinese Academy of Sciences,Chengdu610041,China
  • Received:2008-06-30 Revised:2008-09-03 Published:2009-03-12 Published online:2009-01-15
  • Contact: XIA Xian-Chun

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1771

被引次数

15

摘要：

高分子量谷蛋白亚基Bx7的超量表达对提高小麦面筋强度有重要作用。利用反相高效液相色谱(RP-HPLC)和STS标记检测了163份中国和CIMMYT小麦品种(系)的高分子谷蛋白亚基Bx7超量表达基因(Bx7OE)。结果表明，TaBAC1215C06-F517/R964标记和TaBAC1215C06-F24671/R25515标记可分别在含有Bx7OE基因的材料中扩增出447 bp和844 bp的特异带，在不含Bx7OE基因的材料中无相应目标带，两个STS标记的检测结果完全一致。在163份小麦品种(系)中，11份品种(系)含有Bx7OE基因，占总数的6.7%。RP-HPLC与STS标记检测结果一致。利用这两个STS标记可以方便、快速、准确地检测Bx7OE基因。

关键词: 普通小麦(Triticum aestivum L.), RP-HPLC, STS标记, 分子标记辅助选择, Bx7OE

Abstract:

Over-expression of the high molecular weight glutenin subunit (HMW-GS) Bx7 is highly associated with dough strength of wheat (Triticum aestivum L.) flour. A total of 163 Chinese and CIMMYT wheat cultivars and advanced lines were tested by two STS markers and RP-HPLC to understand the presence of HMW-GS gene Bx7OE The results indicated that the markers TaBAC1215C06-F517/R964 and TaBAC1215C06-F24671/R25515 could amplify a 447 bp and a 844 bp PCR fragments, respectively, in the lines with Bx7OE, whereas no PCR products were detected in the lines without Bx7OE. Of the 163 cultivars and lines, specific PCR fragments were amplified in 11 genotypes by the two markers, indicating the presence of Bx7OE in these lines, with a frequency of 6.7%. The results obtained by RP-HPLC were consistent with those revealed by STS markers. These two STS markers could be used to detect the presence of Bx7OE gene in wheat cultivars.

Key words: Common wheat(Triticum aestivum L.), RP-HPLC;STS marker, Marker-assisted selection, Bx7OE

[1]Payne P I, Law C N, Mudd E E. Control by homologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet, 1980, 58: 113–120
[2]Wrigley C W. Giant proteins with flour power. Nature, 1996, 381: 738–739
[3]Gianibelli M C, Larroque O R, MacRitchie F. Biochemical, genetic, molecular characterization of wheat glutenin and its component subunits. Cereal Chem, 2001, 78: 635–646
[4]Payne P I. Genetics of wheat storage proteins and the effect of allelic variation on bread making quality. Annu Rev Plant Physiol, 1987, 38: 141–153
[5]Payne P I, Holt L M, Law C N. Structural and genetical studies on the weight subunits of wheat glutenin. Theor Appl Genet, 1981, 60: 229–236
[6]Shewry P R, Halford N G, Tatham A S. High molecular weight subunits of wheat glutenin. J Cereal Sci, 1992, 15: 105–120
[7]Zhu J-B (朱金宝), Liu G-T(刘广田), Zhang S-Z(张树臻). High and low molecular subunits of glutenin and their relationships with wheat quality. Sci Agric Sin (中国农业科学), 1996, 29(1): 34–39 (in Chinese with English abstract)
[8]Radovanovic N, Cloutier S, Brown D, Humphreys D G, Lukow O M. Genetic variance for gluten strength contributed by high molecular weight glutenin proteins. Cereal Chem, 2002, 79: 843–849
[9]Butow B J, Ma W, Gale K R, Cornish G B, Rampling L, Larroque O, Morell M K, Bekes F. Molecular discrimination of Bx7 alleles demonstrates that a highly expressed high molecular weight glutenin allele has a major impact on wheat flour dough strength. Theor Appl Genet, 2003, 107: 1524–1532
[10]Vawser M J, Cornish G B. Overexpression of HMW glutenin subunit Glu-B1 7x in hexaploid wheat varieties (Triticum aestivum L.). Aust J Agric Res, 2004, 55: 577–588
[11]Lukow O M, Forsyth S A, Payne P I. Over-production of HMW glutenin subunits coded on chromosome 1B in common wheat (Triticum aestivum L.). J Genet Breed, 1992, 46: 187–192
[12]D’Ovidio R, Masci S, Porceddu E, Kasarda D. Duplication of the high-molecular weight glutenin subunit gene in bread wheat (Triticum aestivum L.) cultivar Red River 68. Plant Breed, 1997, 116: 525–531
[13]Butow B J, Gale K R, Ikea J, Juhasz A, Bedo Z, Tamas L, Gianibelli M C. Dissemination of the highly expressed Bx7 glutenin subunit (Glu-B1al allele) in wheat as revealed by novel PCR markers and RP-HPLC. Theor Appl Genet, 2004, 109: 1525–1535
[14]Cloutier S, Banks T, Nilmalgoda S. Molecular understanding of wheat evolution at the Glu-B1 locus. In: Proceedings of the International Conference on Plant Genomics and Biotechnology: Challenges and Opportunities, Raipur, India, 2005. p 40
[15]Ragupathy R, Naeem H A, Reimer E, Lukow O M, Sapirstein H D, Cloutier S. Evolutionary origin of the segmental duplication encompassing the wheat Glu-B1 encoding the overexpressed Bx7 (Bx7OE) high molecular weight glutenin subunit. Theor Appl Genet, 2007, 116: 283–296
[16]Jiang N, Bao S, Zhang X, Eddy S R, Wessler S R. Pack-MULE transposable elements mediate gene evolution in plants. Nature, 2004, 431: 569–573
[17]Morgante M, Brunner S, Pea G, Fengler K, Zuccolo A, Rafalski A. Gene duplication and exon shuffling by helitron-like transposon generate intraspecies diversity in maize. Nat Genet, 2005, 37: 997–1002
[18]Liu L, He Z H, Yan J, Zhang Y, Xia X C, Pe?a R J. Allelic variation at the Glu-1 and Glu-3 loci, presence of 1B·1R translocation, and their effects on mixographic properties in Chinese bread wheats. Euphytica, 2005, 142: 197–204
[19]Singh N K, Shepherd K W, Cornish G B. A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. J Cereal Sci, 1991, 14: 203–208
[20]Payne P I, Lawrence G J. Catalogue of alleles for the complex loci, Glu-A1, Glu-B1 and Glu-D1, which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res Commun, 1983, 11: 29–35
[21]Zhang P-P(张平平), Zhang Y(张勇), Xia X-C(夏先春), He Z-H(何中虎). Protocol establishment of reversed-phase high-performance liquid chromatography (RP-HPLC) for analyzing wheat gluten protein. Sci Agric Sin (中国农业科学), 2007, 40(5): 1002–1009 (in Chinese with English abstract)
[22]Devos K M, Gale M D. The use of random amplified polymorphic DNA markers in wheat. Theor Appl Genet, 1992, 84: 567–572
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