作物学报 ›› 2017, Vol. 43 ›› Issue (11): 1622-1631.doi: 10.3724/SP.J.1006.2017.01622
姚姝,陈涛,张亚东,朱镇,赵庆勇,周丽慧,赵凌,赵春芳,王才林
YAO Shu, CHEN Tao,ZHANG Ya-Dong,ZHU Zhen,ZHAO Qing-Yong,ZHOU Li-Hui,ZHAO Ling,ZHAO Chun-Fang,WANG Cai-Lin*
摘要:
近年来,优良食味粳稻品种南粳46、南粳5055和南粳9108在江苏等地大面积推广,促进了优质稻米产业的发展。但这些品种均不抗稻瘟病,且缺乏适合在淮北地区种植的中熟中粳型优良食味粳稻品种。本研究以同时携带稻瘟病抗性基因Pi-ta和Pi-b的江苏抗病、高产粳稻品种武粳15为母本,携带低直链淀粉含量基因Wx-mq的优良食味粳稻品种南粳5055为父本配置杂交组合进行聚合育种。利用Pi-ta和Pi-b基因的分子标记多重PCR体系以及Wx-mq基因的四引物扩增受阻突变体系PCR检测技术,分别在不同的分离世代对目标基因位点进行检测,结合田间多代选育、抗性鉴定和籽粒胚乳外观鉴定,成功地将Pi-ta、Pi-b和Wx-mq基因聚合于一体,选育出稻瘟病抗性好、食味品质优、产量高的水稻新品系“南粳0051”,适合在江苏省淮北地区种植。本研究将三套自主研发的PCR检测体系成功应用于分子标记辅助选择,不仅为水稻多基因聚合育种提供了快捷、高效的选择方法,也为水稻抗病、优质育种创制了新的种质资源。
| [1] 王世维, 郑文静, 赵家铭, 魏松红, 王妍, 赵宝海, 刘志恒. 辽宁省稻瘟病菌无毒基因型鉴定及分析. 中国农业科学, 2014, 47: 462–472 Wang S W, Zheng W J, Zhao J M, Wei S H, Wang Y, Zhao B H, Liu Z H, Identification and analysis of Magnaporthe oryzae avirulence genes in Liaoning province. Sci Agric Sin, 2014, 47: 462–472 (in Chinese with English abstract) [2] 李祥晓, 王倩, 罗生香, 何云霞, 朱苓华, 周永力, 黎志康. 黑龙江省稻瘟病菌无毒基因分析及抗病种质资源筛选. 作物学报, 2012, 38: 2192–2197 Li X X, Wang Q, Luo S X, He Y X, Zhu L H, Zhou Y L, Li Z K. Analyzing avirulence genes of Magnaporthe oryzae from Heilongjiang province and screening rice germ plasm with resistance to blast fungus. Acta Agron Sin, 2012, 38: 2192–2197 (in Chinese with English abstract) [3] Shen M G, Lin J Y. The economic impact of rice blast disease in China. In: Zeigler R S, Leong S A, Teng P S. Rice Blast Disease. England, UK: CAB Internationa1, 1994. pp 321–331 [4] Hittalmani S, Parco A, Mew T V', Zeigler R S, Huang N. Fine mapping and DNA marker assisted pyramiding of the three major genes for blast resistance in rice. Theor Appl Genet, 2000, 100: 1121–1128 [5] 时克, 雷财林, 程治军, 许兴涛, 王久林, 万建民. 稻瘟病抗性基因Pi-ta和Pi-b在我国水稻主栽品种中的分布. 植物遗传资源学报, 2009, 10: 21–26 Shi K, Lei C L, Cheng Z J, Xu X T, Wang J L, Wan J M. Distribution of two blast resistance genes Pi-ta and Pi-b in major rice cultivars in China. J Plant Genet Resour, 2009, 10: 21–26 (in Chinese with English abstract) [6] Bryan G T, Wu K S, Farrall L, Jia Y I, Hershey H P, McAdams S A, Fault K N, Donaldson G K, Renato T, Valent B. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta. Plant Cell, 2000, 12: 2033–2045 [7] 陈涛, 骆名瑞, 张亚东, 朱镇, 赵凌, 赵庆勇, 周丽慧, 姚姝, 王才林. 利用四引物扩增受阻突变体系PCR技术检测水稻低直链淀粉含量基因Wx-mq. 中国水稻科学, 2013, 27: 529–534 Chen T, Luo M R, Zhang Y D, Zhu Z, Zhao L, Zhao Q Y, Zhou L H, Yao S, Wang C L. Detection of Wx-mq gene for low-amylose content by tetra-primer amplification refractory mutation system PCR in rice. Chin J Rice Sci, 2013, 27: 529–534 (in Chinese with English abstract) [8] 朱昌兰, 沈文彪, 翟虎渠, 万建民. 水稻低直链淀粉含量基因育种利用的研究进展. 中国农业科学, 2004, 37: 81–88 Zhu C L, Shen W B, Zhai H Q, Wan J M. Progresses in researches of the application of low-amylose content rice gene for breeding. Sci Agric Sin, 2004, 37: 81–88 (in Chinese with English abstract) [9] 姚姝, 陈涛, 张亚东, 朱镇, 赵庆勇, 赵凌, 周丽慧, 王才林. 利用分子标记辅助选择聚合水稻暗胚乳突变基因Wx-mq和抗条纹叶枯病基因Stv-bi. 中国水稻科学, 2010, 24: 341–347 Yao S, Chen T, Zhang Y D, Zhu Z, Zhao Q Y, Zhao L, Zhou L H, Wang C L. Pyramiding of translucent endosperm mutant Wx-mq gene and Stv-bi for resistance to rice stripe virus by Marker-assisted selection in rice (Oryza sativa L.). Chin J Rice Sci, 2010, 24: 341–347 (in Chinese with English abstract) [10] 王才林, 张亚东, 朱镇, 陈涛, 赵凌, 林静. 通过分子标记辅助选择培育优良食味水稻新品种. 中国水稻科学, 2009, 23: 25–30 Wang C L, Zhang Y D, Zhu Z, Chen T, Zhao L, Lin J. Breeding of a new rice variety with good eating quality by marker assisted selection. Chin J Rice Sci, 2009, 23: 25–30 (in Chinese with English abstract) [11] 王才林, 张亚东, 朱镇, 陈涛, 赵庆勇, 赵凌, 周丽慧, 姚姝. 优良食味粳稻新品种南粳5055的选育及利用. 农业科技通讯, 2012, (2): 84–87 Wang C L, Zhang Y D, Zhu Z, Chen T, Zhao Q Y, Zhao L, Zhou L H, Yao S. Breeding and application of a new rice variety Nanjing 5055 with good eating quality. Bull Agric Sci & Technol, 2012, (2): 84–87 (in Chinese) [12] 王才林, 张亚东, 朱镇, 姚姝, 陈涛, 赵庆勇, 周丽慧, 赵凌. 优良食味粳稻新品种南粳9108的选育与利用. 江苏农业科学, 2013, 41(9): 86–88 Wang C L, Zhang Y D, Zhu Z, Yao S, Chen T, Zhao Q Y, Zhou L H, Zhao L. Breeding and application of a new rice variety Nanjing 9108 with good eating quality. Jiangsu Agric Sci, 2013, 41(9): 86–88 (in Chinese) [13] 姚姝, 刘燕清, 张亚东, 朱镇, 陈涛, 赵庆勇,周丽慧, 赵春芳, 于新, 王才林. 水稻抗稻瘟病基因Pi-ta和Pi-b多重PCR体系的构建与应用. 作物学报, 2014, 40: 1565–1571 Yao S, Liu Y Q, Zhang Y D, Zhu Z, Chen T, Zhao Q Y, Zhao L, Zhou L H, Zhao C F, Yu X, Wang C L. Establishment and application of multiplex PCR system for two blast resistance genes Pi-ta and Pi-b in rice. Acta Agron Sin, 2014, 40: 1565–1571 (in Chinese with English abstract) [14] Li W, Lei C L, Cheng Z J, Jia Y L, Huang D Y, Wang J L, Wang J K, Zhang X, Su N, Guo X P, Zhai H Q, Wan J M. Identification of SSR markers for a broad-spectrum blast resistance gene Pi20(t) for marker-assisted breeding. Mol Breed, 2008, 22: 141–149 [15] 王忠华, 贾育林, 吴殿星, 夏英武. 水稻抗稻瘟病基因Pi-ta的分子标记辅助选择. 作物学报, 2004, 30: 1259–1265 Wang Z H, Jia Y L, Wu D X, Xia Y W. Molecular marker-assisted selection of the rice blast resistance gene Pi-ta. Acta Agron Sin, 2004, 30: 1259–1265 (in Chinese with English abstract) [16] Fjellstrom R, Conaway-Bormans C A, McClung A M, Marchetti M A, Shank A R, Park W D. Development of DNA markers suitable for marker assisted selection of three Pi genes conferring resistance to multiple Pyricularia grlsea pathotypes. Crop Sci, 2004, 44: 1790–1798 [17] 刘洋, 徐培洲, 张红宇, 徐建第, 吴发强, 吴先军. 水稻抗稻瘟病Pi-b基因的分子标记辅助选择与应用. 中国农业科学, 2008, 41: 9–14 Liu Y, Xu P Z, Zhang H Y, Xu J D, Wu F Q, Wu X J. Marker-assisted selection and application of blast resistant gene Pi-b in rice. Sci Agric Sin, 2008, 41: 9–14 (in Chinese with English abstract) [18] 罗楚平, 倪磊, 陈志谊, 刘永锋, 刘邮洲, 聂亚锋. 水稻稻瘟病接种技术及2009年江苏省区试品种抗性鉴定. 江苏农业科学, 2009, (6): 178–179 Luo C P, Ni L, Chen Z Y, Liu Y F, Liu Y Z, Ne Y F. Inoculation technology and identification of resistance to rice blast in regional tests in 2009 of Jiangsu Province. Jiangsu Agric Sci, 2009, (6): 178–179 (in Chinese) [19] Huang N, Angeles E R, Domingo J, Magpantay G, Singh S, Zhang G, Kunaravdiel N, Bennett J, Khush G S. Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR. Theor Appl Genet, 1997, 95: 313–320 [20] Sugiura N, Tsuji T, Kato T, Saka N, Touyama T, Hayano S Y, Izawa T. Molecular marker assisted selection in a recurrent backcross breeding for the incorporation of resistance to rice stripe virus and panicle blast in rice (Oryza sativa L.). Breed Res, 2004, 6: 143–148 [21] Narayanan N N, Baisakh N, Cruz C M V, Gnanamanickam S S, Datta K, Datta S K. Molecular breeding for the development of blast and bacterial blight resistance in rice cv.IR50. Crop Sci, 2002, 42: 2072–2079 [22] Jiang G H, Xu C G, Tu J M, Li X H, He Y Q, Zhang Q F. Pyramiding of insect-and disease-resistance genes into an elite indica. cytoplasm male sterile restorer line of rice ‘Minghui 63’. Plant Breed, 2004, 123: 112–116 [23] 倪大虎, 易成新, 李莉, 汪秀峰, 张毅, 赵开军, 王春连, 章琦, 王文相, 杨剑波. 分子标记辅助培育水稻抗白叶枯病和稻瘟病三基因聚合系. 作物学报, 2008, 34: 100–105 Ni D H, Yi X C, Li L, Wang X F, Zhang Y, Zhao K J, Wang C L, Zhang Q, Wang W X, Yang J B. Developing rice lines resistant to bacterial blight and blast with Molecular marker-assisted selection. Acta Agron Sin, 2008, 34: 100–105 (in Chinese with English abstract) [24] 柳武革, 王丰, 金素娟, 朱小源, 李金华, 刘振荣, 廖亦龙, 朱满山, 黄慧君, 符福鸿, 刘宜柏. 利用分子标记辅助选择聚合Pi-1和Pi-2基因改良两系不育瘟病. 作物学报, 2008, 34: 1128–1136 Liu W G, Wang F, Jin S J, Zhu X Y, Li J H, Liu Z R, Liao Y L, Zhu M S, Huang H J, Fu F H, Liu Y B. Improvement of rice blast resistance in TGM S Line by pyramiding of Pi-1and Pi-2 through molecular marker-assisted selection. Acta Agron Sin, 2008, 34: 1128–1136 (in Chinese with English abstract) |
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