作物学报 ›› 2017, Vol. 43 ›› Issue (11): 1611-1621.doi: 10.3724/SP.J.1006.2017.01603
申聪聪1,2,朱亚军2,陈凯2,陈慧珍3,吴志超1,2,孟丽君2,徐建龙1,2,4,*
SHEN Cong-Cong1,2,ZHU Ya-Jun2,CHEN Kai2,CHEN Hui-Zhen3,WU Zhi-Chao1,2,MENG Li-Jun2,XU Jian-Long1,2,4,*
摘要:
利用多亲本高代互交系(multi-parent advanced generation inter-cross, MAGIC)群体(DC1、DC2和8way)及其复合群体DC12 (DC1+DC2)和RMPRIL (DC1+DC2+8way)进行关联分析定位水稻抽穗期和株高QTL。2015年和2016年分别在江西和深圳收集3个MAGIC群体抽穗期数据, 2016年在两地收集株高数据, 结合Rice 55K SNP芯片进行基因分型, 利用关联分析方法检测到3个影响抽穗期的主效QTL (qHD3、qHD6和qHD8), 分别位于第3、第6和第8染色体, 且分别与已知抽穗期基因DTH3、Hd3a和Ghd8在同一区域。检测到5个影响株高的QTL (qPH1.1、qPH1.2、qPH1.3、qPH4和qPH6), 其中qPH1.1和qPH1.2位于已知基因Psd1和sd1附近, 其余3个QTL为影响株高的新位点, 但仅在1个群体和单个环境下被检测到, QTL表达受遗传背景和环境影响大。不同MAGIC群体定位抽穗期和株高的效果不同, 在8亲本MAGIC群体8way及复合群体DC12和RMPRIL分别检测到5、5和6个抽穗期和株高QTL, 明显多于4亲本群体DC1的2个和DC2的4个, 而且作图的精度更高, 表现在定位到的QTL显著水平高和与已知基因距离更近, 尤其是复合群体的联合分析(如DC12和RMPRIL)的作图优势更为明显。研究结果为抽穗期和株高有利基因挖掘奠定了基础, 同时为分子育种提供材料和有利信息。
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