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作物学报 ›› 2009, Vol. 35 ›› Issue (6): 1038-1043.doi: 10.3724/SP.J.1006.2009.01038

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用永久F2群体定位小麦株高的QTL

王岩，李卓坤，田纪春*   

  1. 山东农业大学/国家作物生物学重点实验室小麦品质育种室，山东泰安271018
  • 收稿日期:2008-11-27 修回日期:2009-02-17 出版日期:2009-06-12 网络出版日期:2009-04-16
  • 通讯作者: 田纪春，E-mail:jctian@sdau.edu.cn;Tel:0538-8242040
  • 基金资助:

    本研究由国家基础研究重大发展计划（973计划）项目（2009CB118301）和国家高技术研究发展计划（863计划）项目（2006AA1021E9）资助。

Detection of Quantitative Trait Loci for Plant Height Using an Immortalized F2 Population in Wheat

WANG Yan,LI Zhuo-Kun,TIAN Ji-Chun*   

  1. Group of Quality Wheat Breeding of Key Laboratory of Crop Biology/Shandong Agricultural University Tai'an 271018,China
  • Received:2008-11-27 Revised:2009-02-17 Published:2009-06-12 Published online:2009-04-16
  • Contact: TIAN Ji-Chun，E-mail:jctian@sdau.edu.cn;Tel:0538-8242040

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5024

被引次数

24

摘要：

为研究小麦株高的遗传机制，利用DH群体构建了一套包含168个杂交组合的小麦永久F2群体, 并于2007年种植于山东泰安和山东聊城。构建了一套覆盖小麦21条染色体的遗传连锁图谱并利用该图谱的324个SSR标记对小麦株高进行QTL定位研究，使用基于混合线性模型的QTLNetwork 2.0软件进行QTL分析。在永久F2群体中定位了7个株高QTL，包括4个加性QTL，一个显性QTL，一对上位性QTL，共解释株高变异的20%，其中位于4D染色体的qPh4D，具有最大的遗传效应，贡献率为7.5%；位于2D 染色体显性效应位点qPh2D，可解释1.6%的表型变异；位于5B~6D染色体上位效应位点，可解释1.7%的表型变异。还发现加性效应、显性效应和上位效应对小麦株高的遗传起重要作用，并且基因与环境具有互作效应，结果表明利用永久F2群体进行QTL定位研究的方法有助于分子标记辅助育种。

关键词: 小麦, 永久F2群体, 株高, 数量性状位点

Abstract:

To study the genetic mechanism of wheat plant height, a set of doubled haploid (DH) lines were used to construct an immortalized F2 (IF2) population comprising 168 different crosses. The IF2 population was evaluated for plant height in 2007 cropping seasons in Tai’an and Liaocheng, Shandong province. Linkage map was constructed with 324 SSR markers covering the whole wheat genome, including 284 SSR, 37 ESTs loci, 1 ISSR loci and 2 HMW-GS loci, was constructed. This linkage map covered a total length of 2 485.7 cM with an average distance of 7.67 cM between adjacent markers. QTL analyses were performed using the software QTLNetwork version 2.0 based on the mixed linear model at P < 0.05. Four additive QTLs, 1 dominance QTL and pair of epistatic QTLs were detected, the total QTL effects detected for the plant height explained 20% of the phenotypic variation. One QTL qPh4D for plant height was identified on chromosome 4D, was identified on chromosome 2D, explaining 7.5% of the phenotypic variances. Dominance effect loci qPh2D was identified on chromosome 2D, explaining 1.6% of the phenotypic variances；Epistatic effects of loci was identified on chromosome 5B–6D, explaining 1.7% of the phenotypic variances . The results indicate additive effects, dominance effects and epistatic effects are important in genetics of wheat for plant height, which are also subjected to environmental modifications. These results further demonstrate that the use of IF2 groups QTL positioning research methods contribute to the molecular marker-assisted breeding.

Key words: Common wheat, IF2, Plant height, QTL


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