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作物学报 ›› 2009, Vol. 35 ›› Issue (10): 1909-1915.doi: 10.3724/SP.J.1006.2009.01909

• 耕作栽培·生理生化 • 上一篇    下一篇

茶树体内铝形态及铝累积特性

孙婷1,刘鹏1,*,郑人卫1,2,谢忠雷3,罗虹1   

  1. 1浙江师范大学植物学实验室，浙江金华321004；2浙江师范大学化学系，浙江金华321004；3吉林大学环境与资源学院，吉林长春130026
  • 收稿日期:2009-02-18 修回日期:2009-07-23 出版日期:2009-10-12 网络出版日期:2009-09-10
  • 通讯作者: 刘鹏, E-mail: sky79@zjnu.cn; Tel: 13566780990
  • 基金资助:

    本研究由国家自然科学基金资助项目（30540056，40573052）和浙江省自然科学基金资助项目（303461，504135）资助

Forms and Accumulation of Aluminum in Tea Plant(Camellia sinensis)

SUN Ting1,LIU Peng1,*,ZHENG Ren-Wei1,2,XIE Zhong-Lei3,LUO Hong1   

  1. 1Key Laboratory of Botany,Zhejiang Normal University,Jinhua 321004,China;2Department of Chemistry,Zhejiang Normal University,Jinhua321004,China;3College of Entironment and Resource,Jilin University,Changchun 130026,China
  • Received:2009-02-18 Revised:2009-07-23 Published:2009-10-12 Published online:2009-09-10
  • Contact: LIU Peng, E-mail: sky79@zjnu.cn; Tel: 13566780990

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1606

被引次数

23

摘要：

设置不同Al3+浓度对青茶进行50 d处理，调查茶树铝含量和铝的化学配位形态。结果表明，茶树体内的铝大多以有机态或螯合态形式存在；茶树老叶具有高积累铝的特性，但以5 mmol L-1铝处理时，运输到叶片的铝减少，积累于茶树根部的铝增多。利用27Al NMR技术检测表明，茶树各器官中普遍存在Al13的强烈共振吸收峰；在各器官中还出现–0.38×10-6处和–0.17×10-6处的微弱吸收峰，为目前尚未检出的铝络合物形式；在5 mmol L-1 Al3+处理下，青茶老叶中含有更多的Al-复合物，包括Al-草酸盐(1∶2)、Al-草酸盐(1∶2)和Al-磷酸复合物，说明茶树体中的铝通过与其他物质形成络合物以降低铝的毒性。

关键词: 27A1NMR, 茶树, 铝形态, 铝累积

Abstract:

Aluminum toxicity is a major limiting factor affecting yield and quality of crops in acid soil. Camellia sinensis is an Al-accumulating plant grown healthily even in strong acid soil with high aluminum content. The study of the forms and accumulation of aluminum will be helpful to reveal the Al-toleranting mechanism of tea plant. In this paper, the content of Al3+ in tea plants was determined after Camellia sinensis wastreated for 50 d with different concentrations of Al3+ in culture solution. Besides, the forms of Al were analyzed in the roots, stems and leaves of tea plants by the nondestructive 27Al NMR with high accuracy. The old leaves of tea plant were the major Al-accumulating organ, whereas, roots would take the position of it and accumulate more aluminum when the plant was treated with 2 mmol L-1 Al3+. 27Al NMR test showed that the dissoluble aluminum in tea root, stem and leaf existed mainly in the form of [AlO4Al12(OH)24(H2O)12]7+(Al13), whose chemical shift was 63×10-6, and it was the first discovery in tea plant. Besides of Al13, there were two weak absorption peaks of –0.38×10-6 and –0.17×10-6 in three organs tested, which wereundetected aluminum complexes. Other three complexes appeared in old leaf when treated by 5 mmol L-1 Al3+, which were Al-oxalate (1:1), Al-oxalate (1:2), and Al-phosphate, and the chemical shifts were respectively 6.4×10-6, 12.7×10-6 and –6.2×10-6. In summary, most of Al3+in tea plant exist in chelate complexes or organic matter, which can be considered as one of Al-accumulating and Al-tolerance mechanism of tea plant.

Key words: 27aluminum NMR, Camellia sinensis, Aluminum accumulation


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