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作物学报 ›› 2009, Vol. 35 ›› Issue (10): 1916-1922.doi: 10.3724/SP.J.1006.2009.01916

• 耕作栽培·生理生化 • 上一篇    下一篇

灌浆期干旱胁迫对玉米叶片关系统活性的影响

李耕1，高辉远2,3，赵斌1，董树亭1,3，张吉旺1,3，杨吉顺1，王敬锋1，刘鹏1,3,*   

  1. 1生的农业大学农学院；2山东农业大学生命科学学院；3作物生物学国家重点实验室，山东泰安271018
  • 收稿日期:2009-03-19 修回日期:2009-06-25 出版日期:2009-10-12 网络出版日期:2009-08-07
  • 通讯作者: 刘鹏,E-mail:liup@sdau.edu.cn; Tel:0538-8245838
  • 基金资助:

    本研究由国际科技支撑计划项目（2006BAD02A13-2-2，2007BAD91B04），国家自然科学基金项目（30771282，30871476），山东省良种工程项目资助。

Effects of Drought Stress on Activity of Photosystems in Leaves of Maize at Grain Filling Stage

LI Geng1,GAO Hui-Yuan2,3,ZHAO Bin1,DONG Shu-Ting1,3,ZHANG Ji-Wang1,3,YANG Ji-Shun1,WANG Jing-Feng1,LIU Peng13*   

  1. 1 College of Agronomy, Shandong Agricultural University; 2 College of Life Science, Shandong Agricultural University; 3 State Key Laboratory of Crop Biology, Tai’an 271018, China
  • Received:2009-03-19 Revised:2009-06-25 Published:2009-10-12 Published online:2009-08-07
  • Contact: LIU Peng,E-mail:liup@sdau.edu.cn; Tel:0538-8245838

PDF

2650

被引次数

58

摘要：

以高淀粉玉米品种郑单21为材料，借助叶绿素荧光快速诱导动力学曲线和820 nm光吸收曲线，研究了灌浆期土壤干旱胁迫对玉米籽粒产量和对叶片光系统I (PSI)及光系统II (PS II)活性的影响。两年的研究结果均表明，干旱胁迫显著抑制叶片光合速率(P<0.05)和籽粒产量(P<0.05)。JIP-test分析发现干旱胁迫导致叶绿素荧光快速诱导动力学曲线中的K点和J点上升，表明PS II放氧复合体(OEC)和QA之后的电子传递链受到抑制，且PS II受体侧受抑制的程度大于供体侧。此外，干旱胁迫也显著地抑制PS I的最大氧化还原活性(ΔI/Io)，阻碍光合电子从PS II向PS I的传递，破坏了PS I和PS II的协调性。我们认为干旱胁迫抑制PS I和PS II活性并破坏二者的协调性，是导致导致Pn和籽粒产量下降的重要原因之一。

关键词: 玉米, 干旱胁迫, 叶绿素荧光快速诱导动力学曲线, PSI最大氧化还原活性, 820nm光吸收

Abstract:

At grain filling stage, the effects of drought stress on photosynthetic acivities of photosystem I (PS I) and photosystem II (PS II) in leaves of maize (Zhengdan 21, a cultivar with high starch content) were studied by simultaneously analyzing chlorophyll a fluorescence transient and light absorbance at 820 nm. The results, obtained from two years experiments, demonstrated that the drought stress significantly reduced photosynthesis (P<0.05) and grain yield (P<0.05) of the maize. The K and J steps at fluorescence transient were increased by drought stress, which indicated the inhibition of oxygen- evolving complex (OEC) and electron transport chain after QA in PS II. The acceptor side of PSII was damaged more severely than the donor side of PSII. Furthermore, the maximal oxidation-reduction activity of PS I (ΔI/Io) was also significantly decreased by the drought stress, which inhibited the photosynthetic electron tranport from the PS II to PS I, destructing the coordination between PS I and PS II. We suggest that the inhibition of PS I and PS II and the destruction of the coordination between PS I and PS II by the drought stress is one of the main reasons to cause the decrease in photosyntheis and grain yield of maize.

Key words: Maize, Drought stress, Chlorophyll a fluorescence transient, Maximal oxidation-reduction activity of PS I, 820 nm light absorbance

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