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作物学报 ›› 2009, Vol. 35 ›› Issue (10): 1916-1922.doi: 10.3724/SP.J.1006.2009.01916

• 耕作栽培·生理生化 • 上一篇    下一篇

灌浆期干旱胁迫对玉米叶片关系统活性的影响

李耕1，高辉远2,3，赵斌1，董树亭1,3，张吉旺1,3，杨吉顺1，王敬锋1，刘鹏1,3,*   

  1. 1生的农业大学农学院；2山东农业大学生命科学学院；3作物生物学国家重点实验室，山东泰安271018
  • 收稿日期:2009-03-19 修回日期:2009-06-25 出版日期:2009-10-12 网络出版日期:2009-08-07
  • 通讯作者: 刘鹏,E-mail:liup@sdau.edu.cn; Tel:0538-8245838
  • 基金资助:

    本研究由国际科技支撑计划项目（2006BAD02A13-2-2，2007BAD91B04），国家自然科学基金项目（30771282，30871476），山东省良种工程项目资助。

Effects of Drought Stress on Activity of Photosystems in Leaves of Maize at Grain Filling Stage

LI Geng1,GAO Hui-Yuan2,3,ZHAO Bin1,DONG Shu-Ting1,3,ZHANG Ji-Wang1,3,YANG Ji-Shun1,WANG Jing-Feng1,LIU Peng13*   

  1. 1 College of Agronomy, Shandong Agricultural University; 2 College of Life Science, Shandong Agricultural University; 3 State Key Laboratory of Crop Biology, Tai’an 271018, China
  • Received:2009-03-19 Revised:2009-06-25 Published:2009-10-12 Published online:2009-08-07
  • Contact: LIU Peng,E-mail:liup@sdau.edu.cn; Tel:0538-8245838

PDF

2222

被引次数

58

摘要：

以高淀粉玉米品种郑单21为材料，借助叶绿素荧光快速诱导动力学曲线和820 nm光吸收曲线，研究了灌浆期土壤干旱胁迫对玉米籽粒产量和对叶片光系统I (PSI)及光系统II (PS II)活性的影响。两年的研究结果均表明，干旱胁迫显著抑制叶片光合速率(P<0.05)和籽粒产量(P<0.05)。JIP-test分析发现干旱胁迫导致叶绿素荧光快速诱导动力学曲线中的K点和J点上升，表明PS II放氧复合体(OEC)和QA之后的电子传递链受到抑制，且PS II受体侧受抑制的程度大于供体侧。此外，干旱胁迫也显著地抑制PS I的最大氧化还原活性(ΔI/Io)，阻碍光合电子从PS II向PS I的传递，破坏了PS I和PS II的协调性。我们认为干旱胁迫抑制PS I和PS II活性并破坏二者的协调性，是导致导致Pn和籽粒产量下降的重要原因之一。

关键词: 玉米, 干旱胁迫, 叶绿素荧光快速诱导动力学曲线, PSI最大氧化还原活性, 820nm光吸收

Abstract:

At grain filling stage, the effects of drought stress on photosynthetic acivities of photosystem I (PS I) and photosystem II (PS II) in leaves of maize (Zhengdan 21, a cultivar with high starch content) were studied by simultaneously analyzing chlorophyll a fluorescence transient and light absorbance at 820 nm. The results, obtained from two years experiments, demonstrated that the drought stress significantly reduced photosynthesis (P<0.05) and grain yield (P<0.05) of the maize. The K and J steps at fluorescence transient were increased by drought stress, which indicated the inhibition of oxygen- evolving complex (OEC) and electron transport chain after QA in PS II. The acceptor side of PSII was damaged more severely than the donor side of PSII. Furthermore, the maximal oxidation-reduction activity of PS I (ΔI/Io) was also significantly decreased by the drought stress, which inhibited the photosynthetic electron tranport from the PS II to PS I, destructing the coordination between PS I and PS II. We suggest that the inhibition of PS I and PS II and the destruction of the coordination between PS I and PS II by the drought stress is one of the main reasons to cause the decrease in photosyntheis and grain yield of maize.

Key words: Maize, Drought stress, Chlorophyll a fluorescence transient, Maximal oxidation-reduction activity of PS I, 820 nm light absorbance

[1] Chaves M M and, Oliveira M M. Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture. J Exp Bot, 2004, 55: 2365-2384

[2] Chaves M M. Effects of water deficits on carbon assimilation. J Exp Bot, 1991, 42: 1-16

[3] Quick W P, Chaves M M, Wendler R, David M, Rogrigues M L, Passaharinho J A, Pereira J S, Adcock M D, Leegood R C, Stitt M. The effect of water stress on photosynthetic carbon metabolism in four species grown under field conditions. Plant Cell Environ,1992, 15: 25-35

[4] Long S P, Humphries S, Falkowski P G. Photoinhibition of photosynthesis in nature. Ann Rev Plant Physiol Plant Mol Biol, 1994, 45: 633-662

[5] Xu D-Q (许大全). Several problems in the research of plant light stress. Plant Physiol Commun (植物生理学通讯), 2003, 39(5): 493-495 (in Chinese)

[6] Hu M-J (胡美君), Guo Y-P (郭延平), Shen Y-G (沈允钢), Zhang L-C (张良诚). Environmental regulation of Citrus photosynthesis. Chin J Appl Ecol (应用生态学报), 2006, 17(3): 535-540 (in Chinese with English abstract)

[7] Dai J, Gao H, Dai Y, Zou Q. Changes in activity of energy dissipating mechanisms in wheat flag leaves during senescence. Plant Biol,2004, 6: 171-177

[8] Jiang C D, Gao H Y, Zou Q, Jiang G M, Li L H. Leaf orientation, photorespiration and xanthophyll cycle protect young soybean leaves against high irradiance in field. Environ Exp Bot, 2006, 55: 87-96

[9] Noctor G, Veljovic-Jovanovic S, Driscoll S, Novitakaya L, Foyer C H. Drought and oxidative load in the leaves of C3 plants: a predominant role for photorespiration? Ann Bot, 2002, 89: 841-850

[10] Asada K. The water-water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons. Ann Rev Plant Physiol Plant Mol Biol,1999, 50: 601-639

[11] Chen H X, Li W J, An S Z, Gao H Y. Dissipation of excess energy in Mehler-peroxidase reaction in Rumex leaves during salt shock. Photosynthetica, 2004, 42: 117-122

[12] Müller M, Li X P, Niyogi K K. Non-photochemical quenching: a response to excess light energy. Plant Physiol, 2001, 125: 1558-1566

[13] Foyer C H, Noctor G. Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. Plant Cell Environ, 2005, 28: 1056-1071

[14] Wang X-W(王孝威), Duan Y-H(段艳红), Cao H(曹慧), Zheng W-Y(郑王义). The photosynthetic non-stomatal limitation spur-apple young trees under water stress. Acta Bot Boreali-Occident Sin (西北植物学报), 2003, 23(9): 1609-1613 (in Chinese with English abstract)

[15] Cao H(曹慧), Xu X-F(许雪峰), Han Z-H(韩振海), Wang X-W(王孝威), Guo T-Q(郭图强). Changes of physiological characteristic on photosynthesis in Malus seedling leaves during water stress. Acta Hortic Sin (园艺学报),2004, 31(3): 285-290 (in Chinese)

[16] Jiang C D, Gao H Y, Zou Q. Changes of donor and accepter side in photosystem II complex induced by iron deficiency in attached soybean and maize leaves. Photosynthetica, 2003, 41: 267-271

[17] Schansker G, Tóth S Z, Strasser R J. Methylviologen and dibromothymoquinone treatments of pea leaves reveal the role of photosystemIin the Chl a fluorescence rise OJIP. Biochim Biophys Acta, 2005, 1706: 250-261

[18] Ilík P, Schansker G, Kotabov E, Váczi P, Strasser R J, Bart k M. A dip in the chlorophyll fluorescence induction at 0.2-2 s inTrebouxia-possessing lichens reflects a fast reoxidation of photosystemI. A comparison with higher plants. Biochim Biophys Acta, 2006, 1757: 12-20

[19] Schansker G, Srivastava A, Govindjee, Strasser RJ. Characterization of the 820-nm transmission signal paralleling the chlorophyll a fluorescence rise (OJIP) in pea leaves. Functional Plant Biol, 2003, 30: 785-796

[20] Strasser R J, Srivastava A, Tsimilli-Michael M. The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Yunus M, Pathre U, Mohanty P eds. Probing Photosynthesis: Mechanism, Regulation and Adaptation. London: Taylor and Francis Press, 2000, Chapter 25, pp 445-483

[21] Strasser R J, Tsimill-Michael M, Srivastava A. Analysis of the chlorophyll a fluorescence transient. In: Papageorgiou G, Govindjee eds. Advances in Photosynthesis and Respiration. Netherlands: KAP Press, 2004, Chapter 12, pp 1-47

[22] Wu C-A(吴长艾), Meng Q-W(孟庆伟), Zou Q(邹琦), Zhao S-J(赵世杰), Wang W(王玮). Comparative study on the photooxidative response in different wheat cultivar leaves. Acta Agron Sin (作物学报), 2003, 29(3): 339-344 (in Chinese with English abstract)

[23] Krause G H, Weis E. Chlorophyll fluorescence and photosynthesis: the basics. Annu Rev Plant Physiol Plant Mol Biol,1991, 42: 319-349

[24] Maxwell K, Johnson G N. Chlorophyll fluorescence a practical guide. J Exp Bot, 2000, 51: 659-668

[25] Strasser R J, Srivastava A, Govindjee. Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria. Photochem Photobiol, 1995, 61: 32-42

[26] Guissé B, Srivastava A, Strasser R J. The polyphasic rise of the chlorophyll a fluorescence (O-K-J-I-P) in heat stressed leaves. Archs Sci Genève,1995, 48: 147-160

[27] Eggenberg P, Rensburg L V, Krüger H J, Strasser R J. Screening criteria for drought tolerance in Nicotiana tabacum L. derived from the poly phasic rise of the chlorophyll a fluorescence transient (O-J-I-P). In: Mathis P eds. Photosynthesis: from Light to Biosphere. Dordrecht: KAP Press, 1995, Vol. 4: 661-664

[28] Lu C M, Zhang J H. Heat-induced multiple effects on PSII in wheat plants. J Plant Physiol, 1999, 156: 259-265

[29] Pfannschmidt T, Nilsson A, Allen J F. Photosynthetic control of chloroplast gene expression. Nature, 1999, 397: 625-628

[30] Sun S(孙山), Wang S-M(王少敏), Wang J-X(王家喜), Gao H-Y(高辉远). Effects of dehydration in the dark on functions of PSI and PSII in Apricot (Prunus armeniaca L. ‘JinTaiyang’) leaves. Acta Hortic Sin (园艺学报), 2008, 35(1): 1-6 (in Chinese)
[31] Krause G H, Weis E. Chlorophyll fluorescence and photosynthesis: The basis. Ann Rev Plant Physiol Plant Mol Biol, 1991, 42: 313-349
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