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作物学报 ›› 2010, Vol. 36 ›› Issue (08): 1318-1323.doi: 10.3724/SP.J.1006.2010.01318

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用海岛棉染色体片段导入系定位衣分和籽指QTL

朱亚娟,王鹏**,郭旺珍,张天真*   

  1. 南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095
  • 收稿日期:2009-11-02 修回日期:2010-04-20 出版日期:2010-08-12 网络出版日期:2010-05-20
  • 通讯作者: 张天真, E-mail: cotton@njau.edu.cn; Fax: 025-84395307
  • 基金资助:

    本研究由国家重点基础研究计划(973计划)项目(2006CB101708),国家自然科学基金项目(30730067),江苏省自然科学基金(创新学者攀登项目,BK2008036)和高等学校创新引智计划(B08025)资助.

Mapping QTLs for Lint Percentage and Seed Index by Using Gossypium barbadense Chromosome Segment Introgression Lines

 ZHU Ya-Juan,WANG Peng**,GUO Wang-Zhen, ZHANG Tian-Zhen*   

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2009-11-02 Revised:2010-04-20 Published:2010-08-12 Published online:2010-05-20
  • Contact: ZHANG Tian-Zhen, E-mail: cotton@njau.edu.cn; Fax: 025-84395307

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1466

被引次数

26

摘要： 以染色体片段导入系IL-15-5和IL-15-5-1构建的F2和F2:3分离群体，利用SSR标记对数量性状衣分和籽指QTL进行了定位。应用复合区间作图法分析两个组合的774个F2单株和F2:3家系衣分和籽指，检测到2个衣分的QTL，1个籽指的QTL。衣分QTL qLP-15-1在两世代中都被检测到，位于相同的分子标记置信区间JESPR152~NAU3040，置信的遗传距离分别为5.40 cM和3.20 cM；qLP-15-2只在F2:3中被检测到，位于分子标记NAU5302~NAU2901之间，置信的遗传距离为0.08 cM。籽指QTL qSI-15-1在F2和F2:3中都被检测到，分别位于分子标记NAU2814~NAU3040和JESPR152~NAU3040，置信的遗传距离分别为6.70 cM和5.70 cM。利用染色体片段导入系能准确地定位产量组分的QTL，为棉花产量的分子设计育种奠定基础。

关键词: 染色体片段导入系, 棉花, 衣分, 籽指, QTL

Abstract: Seed index (SI) and lint percentage (LP) are very important components, and many researchers have done abundant investigation on them by using different populations. Chromosome segment introgression lines (CSIL), different from the recurrent parent in introgressed segments, are the ideal materials for QTL research and the core of the breeding by design. The F2 and F2:3 populations were developed from CSILs-IL-15-5 and IL-15-5-1, and used in mapping QTLs for lint percentage and seed index with SSR markers. The SI and LP characteristics of the 774 F2 individuals and F2:3 families were analyzed by composite interval mapping (CIM), and one QTL was detected for SI and two QTLs for LP. qSI-15-1 was located between NAU2814 and NAU3040 with confident genetic distance of 6.70 cM in the F2 population and between NAU3040 and JESPR152 with confident genetic distance of 5.70 cM in the F2:3 population. qLP-15-1 detected in the F2 and F2:3 populations was located between NAU3040 and JESPR152 with confident genetic distance of 5.40 cM and 3.20 cM respectively; qLP-15-2 was mapped between NAU5302 and NAU2901 with confident genetic distance of 0.08 cM. In conclusion, the QTLs for yield components in cotton can be mapped using CSILs and provide a base for breeding by design.

Key words: Chromosome segment introgression lines, Cotton, Lint percentage, Seed index, QTL

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