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作物学报 ›› 2010, Vol. 36 ›› Issue (12): 2116-2123.doi: 10.3724/SP.J.1006.2010.02116

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦品系M8003-5抗条锈病基因遗传分析和分子作图

徐中青1,张书英1,王睿1,王文立1,周新力1,尹军良1,陈洁2,井金学1,*   

  1. 1 西北农林科技大学植保学院 / 陕西省农业分子生物学重点实验室，陕西杨凌 712100；2 河北省农林科学院植物保护研究所，河北保定 071000
  • 收稿日期:2010-03-16 修回日期:2010-05-21 出版日期:2010-12-12 网络出版日期:2010-08-30
  • 通讯作者: 井金学, E-mail: jingjinxue@163.com, Tel: 029-87092434
  • 基金资助:

    本研究由教育部高等学校学科创新引智计划(111计划)项目(B07049), “十一五”国家支撑计划重点项目(2006BAD08A05)和小麦条锈病菌毒性变异与条锈病综合治理研究示范项目(200903035-02)资助。

Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Line M8003-5

XU Zhong-Qing1,ZHANG Shu-Ying1,WANG Rui1,WANG Wen-Li1,ZHOU Xin-Li1,Chen Jie2,JING Jin-Xue1,*   

  1. 1 College of Plant Protection / Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling 712100, China; 2Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, China
  • Received:2010-03-16 Revised:2010-05-21 Published:2010-12-12 Published online:2010-08-30
  • Contact: JING Jin-Xue,E-mail:jingjinxue@163.com,Tel:029-87092434

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摘要： 为了利用小麦抗条锈病品系M8003-5中的抗病基因，用当前7个流行的条锈菌生理小种对小麦品系M8003-5的抗条锈性进行了鉴定，发现该品种对当前的各优势小种均有良好抗性。在温室内以病菌小种Su11-4对M8003-5在进行苗期抗条锈性鉴定和遗传分析，初步确定M8003-5对Su11-4的抗性由1对显性基因控制，位于7DS上的SSR标记Xbarc5、Xwmc463、Xwmc405、Xbarc126、Xgwm295、Xgwm44、Xwmc702、Xwmc438、Xwmc121、Xgwm111和Xbarc121与该基因连锁，最近的为Xwmc702和Xwmc438，遗传距离分别为3.5 cM和4.3 cM。分子标记及其相关分析表明，此基因可能来自黑麦，与已定位于7D染色体上的抗病基因不同，暂命名为YrM8003。利用与其紧密连锁的标记Xwmc702和Xwmc438测黄淮麦区43个主栽品种，结果显示，有20%的品种具有与YrM8003基因相同的标记位点。这一结果有助于YrM8003在抗条锈病育种的应用。

关键词: 小麦品系M8003-5, 条锈病抗性, 遗传分析, SSR, 分子标记辅助选择

Abstract: Strip rust, caused by Puccinia striiformis West. f. sp. tritici (Pst) is a worldwide disease in wheat (Triticum aestivum L.) production. Resistance breeding is constantly pursued for decades to tackle the variations of prevalent Pst races. Wheat genetic resources carrying novel resistance genes always receive great attentions and the utilization study generally initiates from the identification and genetic analysis of the resistance genes. Wheat line M8003-5 is selected from the hybrid progenies of the common wheat variety Chinese Spring and Secale cereale L., which exhibits not only high resistance to Pst but also high yield, early maturity, and resistance to drought. To map the Pst resistance gene(s) in M8003-5, a segregation population was constructed by crossing M8003-5 and a highly susceptible variety Mingxian 169. Seven prevalent Pst races in China were inoculated in greenhouse at seedling stage to evaluate the infection type of individuals from the F1, F2, and F3 generations. The results showed that M8003-5 was resistant to all races, whereas Mingxian 169 was highly susceptible to all inoculates. Genetic analysis indicated that the resistance of M8003-5 against race Su11-4 was conferred by a dominant gene, which was tentatively designated YrM8003. This gene was linked to simple sequence repeat (SSR) markers Xbarc5, Xwmc463, Xwmc405, Xbarc126, Xgwm295, Xgwm44, Xwmc702, Xwmc438, Xwmc121, Xgwm111, and Xbarc128, which were all located on chromosome arm 7DS. The closest flanking makers were Xwmc702 and Xwmc438 with genetic distance of 3.5cM and 4.3 cM, respectively. Gene YrM8003 differs from any other Pst resistance genes on 7DS, and is probably a novel gene. This gene is primarily inferred to originate fromS. cereale based on analyses on pedigree and molecular markers data. Forty-three wheat cultivars from Huang-Huai Winter Wheat Region were also tested with YrM8003 markers Xwmc702 and Xwmc438, and 20% cultivars were amplified with the target bands. These cultivars require further tests to validate the presence of YrM8003.

Key words: M8003-5, Stripe rust resistance, Genetic analysis, SSR marker, Molecular marker-assisted selection

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