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作物学报 ›› 2011, Vol. 37 ›› Issue (02): 280-285.doi: 10.3724/SP.J.1006.2011.00280

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻多小花小穗突变体mf1的鉴定与基因定位

李云峰,杨正林,凌英华,王楠,任德勇,王增,何光华*   

  1. 西南大学水稻研究所 / 农业部生物技术与作物品质改良重点实验室，重庆 400716
  • 收稿日期:2010-07-08 修回日期:2010-10-05 出版日期:2011-02-12 网络出版日期:2010-12-15
  • 通讯作者: 何光华, E-mail: hegh@swu.edu.cn
  • 基金资助:

    本研究受国家高技术研究发展计划(863计划) (2006AA10Z167), 国家自然科学基金项目(30971559)和重庆市杰出青年基金(2008BA1033)的资助。

Characterization and Gene Mapping of a Spikelet Mutant multi-floret 1(mf1) in Rice

LI Yun-Feng,YANG Zheng-Lin,LING Ying-Hua,WANG Nan,REN De-Yong,WANG Zeng,HE Guang- Hua*   

  1. Rice Research Institute, Southwest University / Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Chongqing 400716, China
  • Received:2010-07-08 Revised:2010-10-05 Published:2011-02-12 Published online:2010-12-15
  • Contact: 何光华, E-mail: hegh@swu.edu.cn

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1641

被引次数

7

摘要： 水稻小穗具确定性，一个小穗内只包含一个可育的小花。本文报道一个多小花小穗突变体，其一个小穗内出现两朵及以上小花，暗示小穗分生组织确定性的丢失；另外，这些小花的花器官也表现不正常，包括外稃的伸长、浆片的缺失和雄蕊的减少。遗传分析表明该突变性状受1个隐性单基因控制，暂被命名为multi-floret 1 (mf1)。利用群体分离分析法(bulked segregation analysis, BSA)，MF1基因被定位在第3染色体上SSR标记PSSR3和RM7576之间，物理距离大约为34 kb，包含4个候选基因。研究结果为MF1基因的图位克隆和功能研究奠定了基础。

关键词: 水稻, 小穗, 多小花, 确定性

Abstract:  Rice has a determinate spikelet producing a fertile floret above two sterile lemmas. In this study, we reported a multi-floret 1 (mf1) mutant, in which spikelet lost the determinacy and produced two or more florets in an alternate phyllotaxy above sterile lemmas. In addition, all the florets showed the defects of floral organs development, such as the elongated leafy lemma, and decreased lodicule/stamen. Genetic analysis indicated that the mf1 trait is controlled by a single recessive gene. By bulked segregation analysis (BSA) and rice SSR molecular maker, the mf1 locus was located between PSSR3 and RM7576 on chromosome 3 with a 34 kb physical distance containing four annotated genes. This result provided a foundation of map-based cloning and function analysis of MF1 gene.

Key words: Rice, Spikelet, multi floret 1, Determinacy

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