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作物学报 ›› 2011, Vol. 37 ›› Issue (04): 717-722.doi: 10.3724/SP.J.1006.2011.00717

• 研究简报 • 上一篇    下一篇

水稻57H突变体glup-t的遗传分析与基因定位

田孟祥1,2,陈涛1,张亚东1,朱镇1,赵庆勇1,周丽慧1,姚姝1,王艳平1,王才林1,*   

  1. 1 江苏省农业科学院粮食作物研究所 / 江苏省优质水稻工程技术研究中心 / 国家水稻改良中心南京分中心，江苏南京210014；2南京农业大学农学院，江苏南京210095
  • 收稿日期:2010-08-16 修回日期:2010-11-28 出版日期:2011-04-12 网络出版日期:2011-02-24
  • 通讯作者: 王才林, E-mail: clwang@jaas.ac.cn, Tel: 025-84390307
  • 基金资助:

    本研究由农业部公益性行业(农业)科研专项(200803056)，江苏省农业科技自主创新基金项目(CX[09]634)，江苏省重大科技支撑计划项目(BE2008354)和江苏省自然科学基金项目(BK2008347)资助。

Genetic Analysis and Mapping of glup-t Gene for 57H Mutant in Rice

TIAN Meng-Xiang1,2,CHEN Tao1,ZHANG YA-Dong1,ZHU Zhen1,ZHAO Qing-Yong1,ZHOU Li-Hui1,YAO Shu1,WANG Yan-Ping1,WANG Cai-Lin1,*   

  1. 1 Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice R&D Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, China; 2 College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2010-08-16 Revised:2010-11-28 Published:2011-04-12 Published online:2011-02-24
  • Contact: 王才林, E-mail: clwang@jaas.ac.cn, Tel: 025-84390307

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摘要： 谷蛋白是水稻胚乳中主要的贮藏蛋白，约占总蛋白的80%。谷蛋白最初在粗糙内质网表面以57 kD前体的形式合成，这些前体经加工转运等过程，最终沉积在第二类蛋白体PB-II中，裂解为成熟的酸碱性亚基。任一谷蛋白转运步骤的缺陷都有可能导致谷蛋白57 kD前体的积累，形成谷蛋白前体增加突变体，即57H突变体。细老鼠牙是一个57H自然突变体，其57 kD谷蛋白前体增加而相应的37~39 kD酸性和22~23 kD碱性亚基减少，此外，该突变体还表现为13 kD醇溶蛋白大大增加。本研究以细老鼠牙与武运粳7号、02428杂交获得的F2群体为材料，对57H突变体进行遗传分析和基因定位，结果表明，细老鼠牙的57H突变性状受1对隐性核基因控制，暂命名为glup-t。利用简单重复序列(simple sequence repeat, SSR)、插入缺失(insertion deletion, Indel)和酶切扩增多态性序列(cleaved amplified polymorphic sequence, CAPS)等分子标记的方法，将该突变基因glup-t定位在水稻第4染色体长臂上的CAPS4-3与Indel4-7、Indel4-8之间，遗传距离均为0.26 cM。

关键词: 水稻, 谷蛋白, 57H突变体, glup-t, 基因定位

Abstract: Glutelins are major storage protein and account for 80% of the total protein found in starchy endosperm in rice. They are synthesized on rough endoplasmic reticulum as 57 kD precursors, and then processed into acidic and basic subunits in PB-II. Mutation of the gene controlling the synthesis of glutelin will result in an formation with high amount of 57 kD glutelin precursor, named 57H mutant. In this study, we reported a novel rice 57H spontaneous mutant, Xilaoshuya, which showed increased amount of 57 kD and 13 kD polypeptides, the decreased amounts of 37–39 kD and 22–23 kD polypeptides. Four F2 segregation populations derived from crosses between Xilaoshuya and Wuyunjing 7, 02428 were used to study the inheritance and gene mapping for 57H mutant. The results suggested that, the mutant trait in Xilaoshuya was controlled by a pair of recessive nuclear gene and designated tentatively as glup-t. By means of molecular marker technique, the glup-t gene was mapped between CAPS4-3 and Indel4-7, Indel4-8 on the long arm of chromosome 4, and all of the genetic distances to the three markers were 0.26 cM.

Key words: Rice, Glutelin, 57H mutant, glup-t, Gene mapping

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