江苏省杂草稻遗传多样性及其起源分析

doi:10.3724/SP.J.1006.2011.01324

摘要/Abstract

摘要： 前期调查研究表明江苏是中国杂草稻危害最严重的省份之一，尤其以苏中地区最甚。因此，本文利用具有多态性的33个共显性SSR标记，分析苏中地区的11个杂草稻种群以及苏南苏北部分杂草稻样品，以揭示江苏省主要杂草稻的遗传多样性及其来源。结果表明苏中地区11个杂草稻群体总的遗传多样性(He)为0.1661，Shannon指数(I)为0.2872；多态位点百分率达到87.88%，等位基因数(Na)为2.1515，有效等位基因数(Ne)为1.2887。其中，泰州的遗传多样性最高而南通地区的最低。杂草稻在地区之间的遗传分化(1%)显著小于杂草稻种群之间的遗传分化(39%)，更小于种群内的遗传分化(60%)。聚类分析和主成分分析结果表明苏中和苏南地区杂草稻以籼型为主，苏北地区存在少量粳型杂草稻；苏中地区的杂草稻与现有栽培稻品种和普通野生稻没有明显的亲缘关系，但与曾经栽培过的杂交水稻品种有关。因此，本研究相对支持江苏省杂草稻可能主要来自栽培稻的基因重组或回复突变等产生野生性状即返祖遗传的假说。苏北地区的杂草稻可能来源于穞稻与现今栽培稻杂交的后代。

关键词: 杂草稻, 分子标记, 遗传多样性, 来源, 微卫星标记

Abstract: The previous investigations on occurrence and harmfulness of weedy rice in Jiangsu province showed the province is one of the most serious infestations in China, especially in the central region of Jiangsu province. Hence, in the paper, 33 pairs of co-dominant SSR makers were used to analyse 11 weedy rice populations in central region and other samples in northern and southern regions of this province. Our main objectives were to reveal genetic diversity and analyse the origin of weedy rice. The result showed that the genetic diversity of the 11 populations in central regions of Jiangsu province is He=0.1661, I =0.2872, percentage of polymorphic loci P=87.88%; average number of alleles Na=2.1515; effective number of alleles Ne=1.2887. Among all of 11 populations, the populations collected from Taizhou city had the maximum value of genetic diversity while that from Nantong had the minimum one. Genetic differentiation among regions (1%) was significantly less than among populations (39%) and within populations (60%). Nei’s genetic distance and UPGMA cluster analysis and principle component analysis(PCA) showed that most weedy rice was indica in central and southern regions of Jiangsu Province and a few of japonica in northern regions in the province. All of weedy rice biotypes in central and southern regions did not related with cultivated rice while related with cultivated hybrid indica rice which was ever planted before. Hence, our research results relatively supported the atavistic hypothesis that weedy rice in Jiangsu probably originated from cultivated rice and varieties (accessions) through gene recombination or reverse mutation which produce wild characters. However, the japonica weedy rice in northern regions might be the offspring of the hybrid between cultivated rice and wild rice Ludao.

Key words: Weedy rice, Molecular marker, Genetic diversity, Origin, SSR

邵菁, 戴伟民, 张连举, 宋小玲, 强胜. 江苏省杂草稻遗传多样性及其起源分析[J]. 作物学报, 2011, 37(08): 1324-1332.

SHAO Jing, DAI Wei-Min, ZHANG Lian-Ju, SONG Xiao-Ling, QIANG Qing. Genetic Diversity and Origin of Weedy Rice in Jiangsu Province, China[J]. Acta Agron Sin, 2011, 37(08): 1324-1332.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2011.01324

https://zwxb.chinacrops.org/CN/Y2011/V37/I08/1324

参考文献

[1]Baki B B, Chin D V, Mortimer M. Wild and Weedy Rice in Rice Ecosystems in Asia—a Review. Los Banos, Philippine: Internationl Rice Research Institute, 2000
[2]Lentini Z, Espinoza A M. Coexistence of weedy rice and rice in tropical America—gene flow and genetic diversity. In: Gressel J ed. Crop Ferality and Volunteerism. Boca Raton,,USA; CRC Press Inc , 2005, pp 305–322
[3]Olsen K M, Caicedo A L, Jia Y L. Evolutionary genomics of weedy rice in the USA. J Integr Plant Biol, 2007, 49: 811–816
[4]Gealy D R. Gene movement between rice (Oryza sativa) and weedy rice (Oryza sativa): a US temperate rice perspective.In: Gressel J ed. Crop Ferality and Volunteerism. Boca Raton: CRC Press, 2005. pp 323–354
[5]Estorninos L E, Gealy D R, Gbur E E, Talbert R E, McClelland M R. Rice and red rice interference: II. Rice response to population densities of three red rice (Oryza sativa) ecotypes. Weed Sci, 2005, 53: 683–689
[6]Burgos N R, Norman R J, Gealy D R, Black H. Competitive N uptake between rice and weedy rice. Field Crops Res, 2006, 99: 96–105
[7]Gealy D R, Tai T H, Sneller C H. Identification of red rice, rice, and hybrid populations using microsatellite markers. Weed Sci, 2002, 50: 333–339
[8]Maneechote C, Jamjod S, Rerkasem B. Invasion of weedy rice in rice fields in Thailand: problems and management. Int Rice Res Notes, 2004, 29: 20–22
[9]Pan X-B(潘学彪), Chen Z-X(陈宗祥), Zuo S-M(左示敏), Zhang Y-F(张亚芳). Origin and control strategy on the weedy rice occured in Jiangsu province. Jiangsu Agric Sci (江苏农业科学), 2007, (4): 52–54 (in Chinese with English abstract)
[10]Dai W-M(戴伟民), Song X-L(宋小玲), Zhang L-J(张连举), Zuo R-L(左然林), Zhang Z(张铮), Li S-S(李淑顺), Cao D(曹旦), Zuo J(左娇), Yang L(杨琳), Liu L-L(刘琳莉), Qiang S(强胜). Investigation of weed rice (Oryza sativa f. spontanea) occurrence in Jiangsu province. J Jiangsu Agric Sci (江苏农业学报), 2009, 25(3): 712–714 (in Chinese with English abstract)
[11]Wu C(吴川), Dai W-M(戴伟民), Song X-L(宋小玲), Qiang S(强胜). Diversity of plant traits of weedy rice in Liaoning and Jiangsu provinces. Biodiversity (生物多样性), 2010, 18(1): 29–36 (in Chinese with English abstract)
[12]Yang L(杨琳), Dai W-M(戴伟民), Qiang S(强胜), Song X-L(宋小玲). Cluster analysis of weedy rice and cultivated rice based on the structural characteristics of leaf lower epidermis. Chin J Rice Sci (中国水稻科学), 2009, 23(5): 495–502 (in Chinese with English abstract)
[13]Zhong W-G(仲维功), Yang J(杨杰), Chen Z-D(陈志德), Tang L-H(汤陵华), Wang C-L(王才林), Shi J-W(施积文), Xiao Y-C(肖跃成). Classification of a ‘weedy rice’ in Yangzhong city of Jiangsu province. J Jiangsu Agric Sci (江苏农业学报), 2006, 22(3): 238–242 (in Chinese with English abstract)
[14]Zhang J, Burgos N R, Kun M, Zhou Y J, Yu L Q, Geng R M. Genetic diversity and relationship of weedy rice in Taizhou city, Jiangsu province, China. Rice Sci, 2008, 15(4): 295–302
[15]Cao Q J, Lu B R, Xia H, Rong J, Sala F, Spada A, Grassi F. Genetic diversity and origin of weedy rice (Oryza sativa f. spontanea) populations found in North-eastern China revealed by simple sequence repeat (SSR) markers. Ann Bot, 2006, 98: 1241–1252
[16]Ma D-R(马殿荣), Li M-B(李茂柏), Sun J(孙健), Wang N(王楠), Xu Z-J(徐正进), Chen W-F(陈温福). Studies on biological diversity and genetic differentiation of Liaoning weedy rice. J Shenyang Agric Univ (沈阳农业大学学报), 2008, 39(3): 265–269 (in Chinese with English abstract)
[17]Suh H S, Sato Y I, Morishima H. Genetic characterization of weedy rice (Oryza sativa L.) based on morpho-physiology, isozymes and RAPD markers. Theor Appl Genet, 1997, 94: 316–321
[18]Cho Y C, Chung T Y, Suh H S. Genetic characteristics of Korean weedy rice (Oryza sativa L.) by RFLP analysis. Euphytica, 1995, 86: 103–110
[19]Heu M H, Cho Y C, Suh H S. Cross affinity of Korean weedy rice to the cultivars Korean. J Crop Sci, 1990, 35: 233–238
[20]Londo J P, Schaal B A. Origins and population genetics of weedy red rice in the USA. Mol Ecol, 2007, 16: 4523–4535
[21]Lu Y-J(卢扬江), Zheng K-L(郑康乐). A simple method for isolation of rice DNA. Chin J Rice Sci (中国水稻科学), 1992, 6(1): 47–48 (in Chinese with English abstract)
[22]Yeh F C, Yang R C. POPGENE Version 1.31,
[2010-12-07] http://www.ualberta/fyeh/popgene, 1999
[23]Rohlf F J. NTSYS: Numerical Taxonomy and Multivariate Analysis System, Version 2.1. New York: Exeter Software, 2000
[24]Peakall R, Smouse P E. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes, 2006, 6: 288–295
[25]MINITAB 16, available at http://www.minitab.com.cn/downloading.htm, Minitab Inc, State College, PA, 1998
[26]Guo X-B(郭勋斌), Gu K-L(顾克礼), Yuan Q(袁秦). The occurrence of winter weedy rice and control. J Anhui Agric Sci (安徽农业科学), 2005, 33(7): 1180–1181 (in Chinese with English abstract)
[27]Chen L J, Lee D S, Song Z P, Suh H S, Lu B R. Gene flow from cultivated rice (Oryza sativa) to its weedy and wild relatives. Ann Bot, 2004, 93: 67–73
[28]Olguin S E, Espinoza A G G, Lobo J A , Esquivel E A M. Assessment of gene flow from a herbicide-resistant indica rice (Oryza sativa L.) to the Costa Rican weedy rice (Oryza sativa) in tropical America: factors affecting hybridization rates and characterization of F-1 hybrids. Transgenic Res, 2009, 18: 633–647
[29]Wei X-H(魏兴华), Yang Z-R(杨致荣), Dong L(董岚), Yu H-Y(余汉勇), Wang Y-P(王一平), Yuan X-P(袁筱萍), Tang S-X(汤圣祥). SSR evidence for taxonomic position of weedy rice‘Ludao’. Sci Agric Sin (中国农业科学), 2004, 37(7): 937–942 (in Chinese with English abstract)
[30]Tang L H, Morishima H. Genetic characterization of weedy rices and the inference on their origins. Breed Sci, 1997, 47: 153-160
[31]Min S-K(闵绍楷), Wu X-Z(吴宪章), Yao C-X(姚长溪), Li L-P(李路平). Rice Cropping Regionalizton in China (中国水稻种植区划). Hangzhou: Zhejiang Science and Technology Press, 1989, pp 81–84 (in Chinese with English abstract)

相关文章 15

[1]	翟胜男, 曹新有, 李豪圣, 李吉虎, 李法计, 刘金栋, 夏先春, 吕莹莹, 马瑞峰, 王颖, 耿洪伟, 刘建军. 小麦Pod-A1、Pod-D1和Pod-2D位点等位变异对籽粒过氧化物酶活性的遗传效应分析[J]. 作物学报, 2026, 52(6): 1593-1603.
[2]	彭佳泺, 李颖, 李丹丹, 杨军宁, 郭学峰, 张文姣, 俞晓雪, 周亚荣, 王振玉, 王彩香, 马雄风, 宿俊吉. 陆地棉I类LBD家族成员鉴定及GhLBD6调控开花期的功能和单倍型分析[J]. 作物学报, 2026, 52(6): 1682-1697.
[3]	张献丰, 郭利建, 李康春, 孔斌雪, 刘玉芳, 车卓, 杨德龙. 小麦ABHD6基因家族鉴定与粒重功能标记开发[J]. 作物学报, 2026, 52(6): 1711-1727.
[4]	杨扬, 常诗惠, 田红丽, 易红梅, 王璐, 任洁, 范亚明, 刘亚维, 王凤格, 赵久然. 不同生态区国审玉米品种的遗传多样性分析[J]. 作物学报, 2026, 52(5): 1352-1364.
[5]	徐建霞, 丁延庆, 曹宁, 程斌, 高旭, 李文贞, 王若若, 王磊, 张立异. 397份高粱种质资源在贵州表型多样性分析及综合评价[J]. 作物学报, 2026, 52(4): 1073-1087.
[6]	田春艳, 陆鑫, 吴才文, 徐超华, 刘家勇, 边芯, 桃联安. 基于荧光SSR的甘蔗创新种质遗传多样性分析及育种潜力评估[J]. 作物学报, 2026, 52(4): 1057-1072.
[7]	侯洁, 付朵朵, 武海峰, 郝宇琼, 郑兴卫, 武棒棒, 周凯, 李晓华, 郑军, 赵佳佳. 山西省小麦地方品种的染色体多样性及遗传效应分析[J]. 作物学报, 2026, 52(3): 746-763.
[8]	王楚锐, 李开祥, 赵志, 肖麓, 唐国永, 赵志刚, 徐亮, 杜德志, 柳海东. 甘蓝型春油菜早花基因BnCRY2功能位点KASP标记的开发及应用[J]. 作物学报, 2026, 52(3): 708-721.
[9]	王粤生, 葛冬冬, 程兰斐, 陈春环, 王长有, 刘新伦, 李停栋, 邓平川, 吉万全, 赵继新. 小麦-华山新麦草二体异代换系16DH25-7的分子细胞遗传学及抗病性鉴定[J]. 作物学报, 2026, 52(2): 433-445.
[10]	梅飘, 刘丁丁, 叶圆圆, 张晨禹, 丁诗琦, 李亚奇, 王培鑫, 梅菊芬, 马春雷. 基于茶树液相功能芯片的白化茶树资源遗传多样性分析[J]. 作物学报, 2025, 51(9): 2358-2370.
[11]	王天译, 杨绣娟, 赵佳佳, 郝宇琼, 郑兴卫, 武棒棒, 李晓华, 郝水源, 郑军. 山西小麦醇溶蛋白多样性及其对面粉品质效应研究[J]. 作物学报, 2025, 51(7): 1784-1800.
[12]	展宗冰, 靳奇峰, 刘迪, 吕迎春, 郭莹, 张雪婷, 虎梦霞, 王尚, 杨芳萍. 甘肃省小麦农家种老芒麦分子鉴定及其重要性状评价[J]. 作物学报, 2025, 51(3): 609-620.
[13]	王浩辰, 王克晶, 韩娟, 李向华. 东南沿海短绒野大豆两种代表性生境自然种群的空间遗传结构特征:种群内取样策略研究[J]. 作物学报, 2025, 51(11): 2875-2885.
[14]	徐晓伟, 冯晶, 王凤涛, 童朝阳, 张建周, 李春盈, 蔺瑞明. 小麦地方品种蚕老麦成株抗条锈病QTL定位[J]. 作物学报, 2025, 51(11): 2933-2943.
[15]	田汉钊, 冯龙婷, 应开, 孟天琪, 武军, 刘玉秀. 外引小麦种质麦谷蛋白亚基组成及评价[J]. 作物学报, 2025, 51(10): 2663-2680.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed