欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2012, Vol. 38 ›› Issue (03): 408-415.doi: 10.3724/SP.J.1006.2012.00408

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻两用核不育系龙S抗稻瘟病主效基因的定位

王建龙1,2，吴立群2，刘建丰1， 戴良英1，刘雄伦1，肖应辉1，谢红军3，刘群恩1，李婷1，贾先勇2，王国梁1,4,*，袁隆平5,*   

  1. 1湖南农业大学，湖南长沙 410128；2湖南金健种业有限责任公司，湖南常德 415000；3湖南省水稻研究所，湖南长沙 410125；4美国俄亥俄州州立大学，俄亥俄州哥伦布 43210；5国家杂交水稻工程技术研究中心，湖南长沙 410125
  • 收稿日期:2011-06-21 修回日期:2011-12-15 出版日期:2012-03-12 网络出版日期:2012-01-04
  • 通讯作者: 袁隆平, E-mail: lpyuan@hhrrc.ac.cn, Tel: 0731-82872998; 王国梁, E-mail: wang.620@osu.edu, Tel: 0731-84638423
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2010AA101306)和湖南农业大学引进人才基金项目(11YJ14)资助?

Mapping of the Resistant Gene to Rice Blast in the Dual Purpose Genic Male Sterile Rice, LongS

WANG Jian-Long1,2,WU Li-Qun2,LIU Jian-Feng1,DAI Liang-Ying1,LIU Xiong-Lun1,XIAO Ying-Hui1,XIE Hong-Jun3,LIU Qun-En1,LI Ting1,JIA Xian-Yong2,WANG Guo-Liang1,4,*,YUAN Long-Ping5,*   

  1. 1Hunan Agricultural University, Changsha 410128, China; 2Hunan Jinjian Seed Industry Co., Ltd, Changde 415000, China; 3Hunan Rice Research Institute, Changsha 410125, China; 4Ohio State University, Columbus 43210, USA; 5China National Hybrid Rice Research and Development Center, Changsha 410125, China
  • Received:2011-06-21 Revised:2011-12-15 Published:2012-03-12 Published online:2012-01-04
  • Contact: 袁隆平, E-mail: lpyuan@hhrrc.ac.cn, Tel: 0731-82872998; 王国梁, E-mail: wang.620@osu.edu, Tel: 0731-84638423

PDF

1802

被引次数

7

摘要： 龙S是一个广谱抗稻瘟病的水稻两用核不育系，利用分子标记技术精细定位其主效抗性基因，对于培育抗稻瘟病水稻新品种具有重要意义。采用来自国内外的41个稻瘟病菌系通过接种鉴定方式对龙S进行了稻瘟病抗谱分析，结果显示龙S的抗性频率为100%，对其中39个菌系表现高水平抗性，与Pi9的携带品种75-1-127抗性频率和抗病级别基本相当。群体遗传分析表明龙S的抗性基因表现为显性遗传方式，对于不同菌系龙S表现出不同的抗病遗传模式，其中龙S对稻瘟菌系318-2的抗性由单基因控制。通过抗病亲本龙S与感病亲本日本晴构建F2分离群体，采用BSA (bulk segregant analysis)及RCA (recessive class analysis)分析方法，将龙S的主效抗病基因精细定位于第9染色体上的SSR标记M1-M2所在的1.31 cM区间，与已克隆的广谱抗稻瘟病基因Pi5位于相邻的染色体区域。抗谱分析表明，龙S与Pi5、Pii单基因系的抗性频率差异明显，抗谱较后二者更广。龙S主效抗性基因的精细定位，为进一步揭示其与Pi5、Pii的等位关系以及通过分子标记辅助选择培育抗病水稻新品种奠定了基础。

关键词: 水稻, 龙S, 稻瘟病, 抗性基因, 基因定位

Abstract: LongS is a dual purpose genic male sterile rice with broad-spectrumresistance to rice blast. The objective of the present study was to identifythe resistance spectrumto rice blast, to analyze thegenetic behavior ofresistance gene, and tomap the major resistancegenes in LongS. LongS had a resistance frequency of 100% inoculatedwith 41strainsofMagnaporthe oryza. Population geneticanalysis showed that theresistance genes in LongSexhibited dominant inheritance, the genetic model of R gene varied depend on the strainsof Magnaporthe oryzae. The main-effect resistant gene to rice blast was fine mapped, by using the bulk segregant analysis (BSA) and recessive class analysis (RCA) methods, with the F2 population derived from the resistant parent of LongS and the susceptible parent of Nipponbare. Asingle resistant gene to the race of 318-2 locatedon the interval flanked by the SSRmarkersof M1 and M2with a genetic distance of 1.3 cM onchromosome 9 were adjacent to the broad-spectrum blast resistance gene, Pi5. Both of the resistance spectrum and resistant frequency of LongS, however, were significantly different to those of resistant gene of Pi5 and Pii. In conclusion, the major-effect resistant gene identified in this study may be a new broad-spectrum blast resistance gene. The DNA markers linked to the new R gene identified in this study should be useful for marker-aided breeding of blast-resistant rice cultivars.

Key words: Rice, LongS, Rice blast, Resistant gene, Gene mapping

[1]Dean R A, Talbot N J, Ebbole D J, Farman M L, Mitchell T K, Orbach M J, Thon M, Kulkarni R, Xu J R, Pan Q H, Read N D, Lee Y H, Carbone I, Brown D, Oh Y Y, Donofrio N, Jeong J S, Soanes D M, Djonovic S, Kolomiets E, Rehmeyer C, Li W, Harding M, Kim S, Lebrun M H, Bohnert H, Coughlan S, Butler J, Calvo S, Ma L J, Nicol R, Purcell S, Nusbaum C, Galagan J E, Birren B W. The genome sequence of the rice blast fungus Magnaporthe grisea. Nature, 2005, 434: 980–986

[2]Liu J L, Wang X J, Mitchell T, Hu Y J, Liu X L, Dai L Y, Wang G L. Recent progress and understanding of the molecular mechanisms of the rice–Magnaporthe oryzae interaction. Mol Plant Pathol, 2010, 11: 419–427

[3]Ballini E, Morel J B, Droc G, Price A, Courtois B, Notteghem J L, Tharreau D. A genome-wide meta-analysis of rice blast resistance genes and quantitative trait loci provides new insights into partial and complete resistance. Mol Plant-Microbe Interact, 2008, 21: 859–868

[4]Lee S, Wamishe Y, Jia Y, Liu G, Jia M H. Identification of two major resistance genes against race IE-1k of Magnaporthe oryzae in the indica rice cultivar Zhe733. Mol Breed, 2009, 24: 127–134

[5]Xiao W M, Yang Q Y, Wang H, Guo T, Liu Y Z, Zhu X Y, Chen Z Q. Identification and fine mapping of a resistance gene to Magnaporthe oryzae in a space-induced rice mutant. Mol Breed, Online First, 31 July 2010, DOI: 10.1007/s11032-010-9481-6

[6]Huang H M, Huang L, Feng G P, Wang S H, Wang Y, Liu J L, Jiang N, Yan W T, Xu L C, Sun P Y, Li Z Q, Pan S J, Liu X L, Xiao Y H, Liu E M, Dai L Y, Wang G L. Molecular mapping of the new blast resistance genes Pi47 and Pi48 in the durably resistant local rice cultivar Xiangzi 3150. Phytopathology, 2011, 101:620–626

[7]Jie C, Shi Y F, Liu W Z, Chai R Y, Fu Y P, Zhuang J Y, Wu J L. A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae. J Genet Genomics, 2011, 38: 209–216

[8]Qu S H, Liu G F, Zhou B, Bellizzi M, Zeng L R, Dai L Y, Han B, Wang G L. The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice. Genetics, 2006, 172: 1901–1914

[9]Suh J P, Roh J H, Cho Y C, Han S S, Kim Y G, Jena K K. The Pi40 gene for durable resistance to rice blast and molecular analysis of Pi40-advanced backcross breeding lines. Phytopathology, 2009, 99: 243–250

[10]Lee S K, Song M Y, Seo Y S, Kim H K, Ko S, Cao P J, Suh J P, Yi G, Roh J H, Lee S, An G, Hahn T R, Wang G L, Ronald P, Jeon J S. Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes. Genetics, 2009, 181: 1627–1638

[11]Chen X W, Shang J J, Chen D X, Lei C L, Zou Y, Zhai W X, Liu G Z, Xu J C, Ling Z Z, Cao G, Ma B T, Wang Y P, Zhao X F, Li S G, Zhu L H. A B-lectin receptor kinase gene conferring rice blast resistance. Plant J, 2006, 46: 794–804

[12]Wu J L, Fan Y Y, Li D B, Zheng K L, Leung H, Zhuang J Y. Genetic control of rice blast resistance in the durably resistant cultivar Gumei 2 against multiple isolates. Theor Appl Genet, 2005, 111: 50-56

[13]Fuentes J L, José Correa-Victoria F, Escobar F, Prado G, Aricapa G, Duque M C, Tohme J. Identification of microsatellite markers linked to the blast resistance gene Pi-1(t) in rice. Euphytica, 2008, 160: 295–304

[14]Zhou B, Qu S H, Liu G F, Maureen D, Hajime S, Lu G D, Maria B, Wang G L. The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea. Mol Plant-Microbe Interact, 2006, 19: 1216–1228

[15]Li W, Lei C L, Cheng Z J, Jia Y L, Huang D Y, Wang J L, Wang J K, Zhang X, Su N, Guo X P, Zhai H Q, Wan J M. Identification of SSR markers for a broad-spectrum blast resistance gene Pi20(t) for marker-assisted breeding. Mol Breed, 2008, 22: 141–149

[16]Berruyer R, Adreit H, Milazzo J, Gaillard S, Berger A, Dioh W, Lebrun M H, Tharreau D. Identification and fine mapping of Pi33, the rice resistance gene corresponding to the Magnaporthe grisea avirulence gene ACE1. Theor Appl Genet, 2003, 107: 1139–1147

[17]Deng Y W, Zhu X D, Shen Y, He Z H. Genetic characterization and fine mapping of the blast resistance locus Pigm(t) tightly linked to Pi2 and Pi9 in a broad-spectrum resistant Chinese variety. Theor Appl Genet, 2006, 113: 705–713

[18]Xu X, Hayashi N, Wang C T, Kato H, Fujimura T, Kawasaki S. Efficient authentic fine mapping of the rice blast resistance gene Pik-h in the Pik cluster, using new Pik-h-differentiating isolates. Mol Breed, 2008, 22: 289–299

[19]Liu J-F(刘建丰), Li C-G(李春庚). Breeding of rice PTGMS line LongS with good disease resistance and grain quality. Hybrid rice(杂交水稻), 2010, 25(3): 3–4 (in Chinese with English abstract)

[20]International rice genome sequencing project. The map-based sequence of the rice genome. Nature, 2005, 436: 793–800

[21]McCouch S R, Teytelman L, Xu Y, Lobos K B, Clare K, Walton M, Fu B, Maghirang R, Li Z, Xing Y, Zhang Q, Kono I, Yano M, Fjellstrom R, DeClerck G, Schneider D, Cartinhour S, Ware D, Stein L. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res, 2002, 9: 199–207

[22]Jeremy D E, Jaroslav J, Megan T S, Ambika B G, Liu B, Hei L, David W G. Development and evaluation of a high-throughput, low-cost genotyping platform based on oligonucleotide microarrays in rice. Plant Methods, 2008, 4: 13

[23]Lincoln S, Daley M, Lander E. Constructing Genetic Maps with MAPMAKER/EXP 3.0, 3rd edn. Whitehead Institute Technical Report, Cambridge, 1992

[24]Yang Q-Z(杨勤忠), Lin F(林菲), Feng S-J(冯淑杰), Wang L(王玲), Pan Q-H(潘庆华). Recent progress on molecular mapping and cloning of blast resistance genes in rice (Oryza sativa L.). Sci Agric Sin (中国农业科学), 2009, 42(5): 1601–1615 (in Chinese with English abstract)

[25]Liu H(刘海), Xiao Y-H(肖应辉), Tang W-B(唐文邦), Deng H-B(邓化冰), Chen L-Y(陈立云). Development and application of a computer-aided selection system for thermo-sensitive genic male sterile rice multiplying site. Acta Agron Sin(作物学报), 2011, 37(5): 755–763 (in Chinese with English abstract)

[26]Yang S-H(杨仕华), Cheng B-Y(程本义), Shen W-F(沈伟峰), Xia J-H(夏俊辉). Progress of application and breeding on two-line hybrid rice in China. Hybrid Rice (杂交水稻), 2009, 24(1): 5–9 (in Chinese with English abstract)
[1] 胡赵, 钱润, 谢丰璞, 应素平. 水稻SPX基因家族鉴定及响应磷处理的表达分析[J]. 作物学报, 2026, 52(6): 1902-1912.
[2] 邹仪妹, 徐敏, 汪海洋, 姚辉, 王加峰, 刘浩, 任代胜. 两系不育系水稻幼苗根系响应盐胁迫的转录因子调控网络鉴定[J]. 作物学报, 2026, 52(6): 1728-1742.
[3] 闫安, 蒋昆炜, 王蓉圆, 田林, 张璐, 王韵, 徐建龙. 水稻剑叶小维管束数基因SVN7的鉴定与克隆[J]. 作物学报, 2026, 52(5): 1364-1372.
[4] 陈伟, 卫万娟, 赵其兵, 常东伟, 余凌波, 翟鹏飞, 冯志明, 陈宗祥, 任仰涛, 杨鹏, 刘海浪, 李珍富, 杨永乐, 金彦刚, 左示敏. 利用CRISPR/Cas9编辑Hd6基因创制优质早熟水稻新种质[J]. 作物学报, 2026, 52(4): 1046-1056.
[5] 石少阶, 刘凯, 陈姿夷, 王卉颖, 李三和, 周雷, 游艾青. 水稻矮化多分蘖基因DMT1的克隆与功能分析[J]. 作物学报, 2026, 52(4): 1022-1034.
[6] 覃奕琰, 付瑶, 苏畅, 李娜, 徐静茹, 程笑然, 张琪, 赵明辉. OsST41调控水稻苗期耐盐性的功能分析[J]. 作物学报, 2026, 52(3): 802-812.
[7] 叶凡, 李帅, 李思宇, 陈云, 窦超银, 刘立军. 不同节水灌溉方式对东北稻区水稻产量和群体质量的影响[J]. 作物学报, 2026, 52(3): 895-907.
[8] 苏爱国, 肖森林, 易红梅, 段赛茹, 王帅帅, 张如养, 邢锦丰, 李春辉, 孙轩, 徐瑞斌, 徐田军, 李志勇, 张勇, 王荣焕, 宋伟, 赵久然. 玉米穗腐病的抗性遗传研究进展与育种应用[J]. 作物学报, 2026, 52(1): 1-13.
[9] 王婵, 吴莹莹, 李文奇, 李霞, 王芳权, 周彤, 杨杰. 基于HRM技术开发水稻抗条纹叶枯病基因STV11功能标记[J]. 作物学报, 2025, 51(9): 2547-2556.
[10] 陈惠莹, 何嘉欣, 朱斌, 黄士轩, 周星佑, 伍君权, 杨美艳. 水稻黄单胞菌噬菌体vB_XaS_HDB2的全基因组分析和生物学特性研究[J]. 作物学报, 2025, 51(8): 2087-2099.
[11] 杨海洋, 吴林宣, 李博纹, 石翰峰, 袁禧龙, 刘金朝, 蔡海荣, 陈诗怡, 郭涛, 王慧. 基于QTL定位发现的OsWRI3调控水稻种子的落粒性[J]. 作物学报, 2025, 51(7): 1712-1724.
[12] 凤舞剑, 冼晓青, 张新钵, 曹丹, 强承魁. 基于转录组和AlphaFold对稻瘟菌经典效应蛋白和水稻受体的快速鉴定[J]. 作物学报, 2025, 51(6): 1480-1488.
[13] 雷松翰, 范骏扬, 车艳奕, 代永东, 郑雨萌, 田维江, 桑贤春, 王晓雯. 水稻内卷叶突变体acl3的鉴定及调控基因的功能分析[J]. 作物学报, 2025, 51(6): 1467-1479.
[14] 李福媛, 杨奕, 马继琼, 许明辉, 林良斌, 孙一丁. 水稻OsPUB4基因克隆、激素诱导表达分析与互作蛋白筛选[J]. 作物学报, 2025, 51(6): 1690-1700.
[15] 李世鹏, 陈才武, 张晶, 吕恬, 傅廷栋, 易斌. 基于改进U-Net++模型的油菜pol TCMS温敏两系育性等级鉴定及温度育性关系的量化研究[J]. 作物学报, 2025, 51(6): 1423-1434.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2