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作物学报 ›› 2012, Vol. 38 ›› Issue (04): 596-605.doi: 10.3724/SP.J.1006.2012.00596

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

国家芝麻区域试验新品种(系)的DNA指纹分析

刘红艳，吴坤，杨敏敏，左阳，赵应忠*   

  1. 中国农业科学院油料作物研究所 / 农业部油料作物生物学与遗传育种重点实验室，湖北武汉 430062
  • 收稿日期:2011-08-17 修回日期:2011-12-19 出版日期:2012-04-12 网络出版日期:2012-02-13
  • 通讯作者: 赵应忠, E-mail: yingzhzh@public.wh.hb.cn
  • 基金资助:

    本研究由国家自然科学基金项目(31101180)，中国农业科学院油料作物研究所所长基金项目(1610172011007)，国家芝麻产业技术体系项目(CARS-15)和国家科技支撑计划项目(2009BADA8B04)资助。

DNA Fingerprinting of Sesame (Sesamum indicum L.) Varieties (Lines) from Recent National Regional Trials in China

LIU Hong-Yan,WU Kun,YANG Min-Min,ZUO Yang,ZHAO Ying-Zhong*   

  1. Key Laboratory of Oil Crops Biology and genetic breeding of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Science, Wuhan 430062, China
  • Received:2011-08-17 Revised:2011-12-19 Published:2012-04-12 Published online:2012-02-13
  • Contact: 赵应忠, E-mail: yingzhzh@public.wh.hb.cn

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1339

被引次数

23

摘要： 以我国近年参加国家芝麻区域试验的43个品种(系)为材料，利用12对SSR核心引物开展DNA指纹分析，初步构建参试品种的DNA指纹图谱，并根据指纹图谱分析了参试品种的特异性和一致性。聚类分析结果表明，12对引物检测到30个标记，可将43个品种完全区分开，来源于同一育种单位的品种遗传基础相近，而不同来源的品种其遗传背景相差较大；以遗传相似系数0.90为划分标准，95%的供试品种具备特异性；以引物位点的一致性为指标，80%的品种具有一致性；分配试验中，86%的单株都能正确地分配回到原来的品种，这些品种的一致性较好，个别品种的单株正确分配率较低，品种一致性差。表明大部分参加国家区试的品种都具有很好的特异性和一致性。

关键词: 芝麻品种, SSR, 特异性, 一致性

Abstract: DNA fingerprinting is an important technique for plant variety identification and protection. In this study, 43 sesame (Sesamum indicum L.) varieties (lines) from recent National Regional Trail were amplificated with 12 pairs of SSR primers, and a total of 30 polymorphic bands were obtained. These molecular markers were further used for variety (lines) distinction and uniformity analysis. Clustering analysis showed that 43 varieties (lines) were completely differed from each other with 30 markers. Varieties (lines) from the same breeding institute tended to share a higher genetic similarity, while varieties (lines) with different origins seemed to be more distinct. With a genetic similarity coefficient threshold of 0.90, 90% of varieties (lines) were distinctive, and 80% of tested varieties (lines) were classified into high uniformity group in consistence with marker loci uniformity, Furthermore, in assignment test, 86% of derivative individuals were correctly corresponding to their original varieties (lines), it indicated that most varieties (lines) have a good uniformity. Overall, the results showed that most of the tested sesame varieties (lines) have good distinction and uniformity, and SSR marker is suitable for variety identification and protection.

Key words: Sesame variety, SSR, Distinctness, Uniformity

[1]Ashri A. Sesame breeding. Plant Breed Rev, 1998, 16: 179–228

[2]Sun J(孙建), Zhang X-R(张秀荣), Zhang Y-X(张艳欣), Che Z(车卓), Huang B(黄波). Analysis on genetic diversity and genetic basis of main sesame cultivars released in China. Sci Agric Sin (中国农业科学), 2009, 42(10): 3421–3431 (in Chinese with English abstract)

[3]Ma S-W(马双武). Research and application progress of seedling marker character in crop breeding and seed purity identification in China. J Plant Genet Resour (植物遗传资源学报), 2011, 12( 2): 297–300 (in Chinese with English abstract)

[4]Mei D-S(梅德圣), Li Y-C(李云昌), Chen Y-F(陈玉峰), Li Y-D(李英德), Xu Y-S(徐育松), Hu Q(胡琼). Seed purity identification of Zhongyouza 12 by peroxidase isozyme and SSR markers in Brassica napus. Chin J Agric Biotechnol (农业生物技术学报), 2010, 18(4): 815–821 (in Chinese with English abstract)

[5]Fang Y-C(方玉春), Zhang X-H(张兴和), Feng D-J(冯德举), Liu J-P(刘俊平), Zhuang B-C(庄炳昌), Liang X-K(梁晓葵). The identification of corn seed purity by using PAGE patterns of salt soluble protein. J Jilin Agric Univ (吉林农业大学学报), 2001, 23(4): 6–10 (in Chinese with English abstract)

[6]Smith J S C, Chin E C L, Shu H, Smith O S. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): Comparison with data from RFLPs and pedigree. Theor App1 Genet, 1997, 95: 163–173

[7]Wang F-G(王风格), Zhao J-R(赵久然), Guo J-L(郭景伦), She H-D(佘花娣), Chen G(陈刚). Comparison of three DNA fingerprint analyzing methods for maize cultivars’ identification. Mol Plant Breed (分子植物育种), 2003, 1(5): 655–661 (in Chinese with English abstract)

[8]Li L(李莉), Yang J-B(杨剑波), Machill D J, Colowit P M. Comparison of different detection methods for rice SSR analysis. Chin J Rice Sci (中国水稻科学), 2000, l4(3): 185–l88 (in Chinese with English abstract)

[9]Li W(李伟), Zheng Y-L(郑有良), Wei Y-M(魏育明), Yan Z-H(颜泽洪), Lan X-J(兰秀锦). Molecular identification of new wheat cultivar Chuannong 16. J Triticeae Crops (麦类作物学报), 2004, 24(1): 6–l0 (in Chinese with English abstract)

[10]Lu G-Y(陆光远), Wu X-M(伍晓明), Zhang D-X(张冬晓), Liu F-L(刘凤兰), Chen B-Y(陈碧云), Gao G-Z(高桂珍), Xu K(许鲲). SSR-based evaluation of distinctness and uniformity of rapeseed (Brassica napus L.) varieties under Chinese National Official Field Tests. Sci Agric Sin (中国农业科学), 2008, 41(1): 32–42 (in Chinese with English abstract)

[11]Luan Y-S(栾雨时), An L-J(安利佳), Wang B-Q(黄百渠), Zhang W-J(张文俊), He M-Y(何孟元). Using simple sequence repeat probe for DNA fingerprints in tomato cultivars. Acta Hort Sin (园艺学报), 1999, 26(1): 51–53 (in Chinese with English abstract)

[12]Cui Y-H(崔艳华), Qiu L-J(邱丽娟), Chang R-Z(常汝镇), Lü W-H(吕文河). Examination of representiveness of the primary core collection in Huanghuai summer sowing soybean (Glycine max) using SSR. J Plant Genet Resour (植物遗传资源学报), 2003, 4(1): 9–l5 (in Chinese with English abstract)

[13]Dixit A, Jin M H, Chung J W, Yu J W, Chung H K, Ma K H, Park Y J, Cho E G. Development of polymorphic microsatellite markers in sesame (Sesamum indicum L.). Mol Ecol Notes, 2005, 5: 736–738

[14]Wei L-B(魏利斌), Zhang H-Y(张海洋), Zheng Y-Z(郑永战), Guo W-Z(郭旺珍), Zhang T-Z(张天真). Development and utilization of EST-derived microsatellites in sesame (Sesamum indicum L.). Acta Agron Sin (作物学报), 2008, 34(12): 2077–2084 (in Chinese with English abstract)

[15]Lu G-Y(陆光远), Yang G-S(杨光圣), Fu T- D(傅廷栋). An effective assay of detecting SSR marker in rapeseed. Chin J Oil Crops Sci (中国油料作物学报), 2003, 25: 79–81 (in Chinese with English abstract)

[16]Zhang B(张鹏), Zhang H-Y(张海洋), Guo W-Z(郭旺珍), Zheng Y-Z(郑永战), Wei L-B(魏利斌), Zhang T-Z(张天真). Genetic diversity analysis of Sesamum indicum L. germplasms using SRAP and EST-SSR markers. Acta Agron Sin (作物学报), 2007, 33 (10): 1696–1702 (in Chinese with English abstract)

[17]Lombard V, Baril C P, Dubreuil P, Blouet F, Zhang D. Genetic relationships and fingerprinting of rapeseed cultivars by AFLP: consequences for varietal registration. Crop sci, 2000, 40: 1417–1425

[18]Piry S, Alapetite A, Cornuet J M, Paetkau D, Baudouin L, Estoup A. GeneClass2: a software for genetic assignment and first-generation migrant detection. J Heredity, 2004, 95: 536–539

[19]Troyer A F, Rocheford T R. Germplasm ownership: related corn inbreds. Crop Sci, 2002, 42: 3–10

[20]LI J-F(李俊芳), Zhang X-Y(张雪原), Sun S-X(孙世贤), Wang S-C(王守才). Analysis of maize variety in national maize main production area using SSR technique: Ⅰ. Evaluation of distinctness and uniformity of maize variety. J Maize Sci (玉米科学), 2006, 14(6): 38–42 (in Chinese with English abstract)

[21]Heckenberger M, van der Voort J R, Melchinger A E, Peleman J, Bohn M. Variation of DNA fingerprints among accessions within maize inbred lines and implications for identification of essentially derived varieties: II. Genetic and technical sources of variation in AFLP data and comparison with SSR data. Mol Breed, 2003, 12: 97–106.

[22]Smith J S C, Smith O S. The description and assessment of distances between inbred lines of maize: II. The utility of morphological, biochemical, and genetic descriptors and a scheme for testing of distinctiveness between inbred lines. Maydica, 1989, 34: 151–161

[23]ASSINSEL. 2000. DUS testing: Phenotype vs. genotype [Online]. ASSINSEL, Position Paper adopted at the Rome Congress in May 2000. http://www.worldseed.org/Position_papers/PhenotypeGenotype.htm [verified 30 Nov. 2004]. International Association of Plant Breeders for the Protection of Plant Varieties (ASSINSEL), Nyon, Switzerland
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