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作物学报 ›› 2013, Vol. 39 ›› Issue (02): 207-215.doi: 10.3724/SP.J.1006.2013.00216

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

油菜半矮杆新品系10D130株型性状的遗传分析

周清元,李军庆,崔翠,卜海东,阴涛,颜银华,李加纳*,张正圣*   

  1. 西南大学农学与生物科技学院 / 南方山地农业教育部工程研究中心 / 西南大学重庆市油菜工程技术研究中心, 重庆 400716
  • 收稿日期:2012-07-21 修回日期:2012-10-09 出版日期:2013-02-12 网络出版日期:2012-12-11
  • 通讯作者: 李加纳, E-mail: ljn1950@swu.edu.cn, Tel: 023-68251950; 张正圣, E-mail: zhangzs@swu.edu.cn, Tel: 023-68250993
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2011AA10A104), 高等学校学科创新引智计划资助(B12006), 国家农业现代产业技术体系建设专项(CARS-13)和重庆市自然科学基金项目(cstc2011jjA80005)资助。

Genetic Analysis of Plant Type in Semi-Dwarf New Line (10D130) of Rapeseed

ZHOU Qing-Yuan,LI Jun-Qing,CUI Cui,BU Hai-Dong,YING Tao,YAN Ying-Hua,LI Jia-Na*,ZHANG Zheng-Sheng*   

  1. College of Agronomy and Biotechnology, South west University / Engineering Research Center of South Upland Agriculture, Ministry of Education / Chongqing Engineering Research Center for Rapeseed, Chongqing 400716, China
  • Received:2012-07-21 Revised:2012-10-09 Published:2013-02-12 Published online:2012-12-11
  • Contact: 李加纳, E-mail: ljn1950@swu.edu.cn, Tel: 023-68251950; 张正圣, E-mail: zhangzs@swu.edu.cn, Tel: 023-68250993

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摘要：

株型改良是油菜高产、优质育种的主攻方向之一。矮杆及半矮杆株型有利于提高植株抗倒伏能力和经济系数、减少收获难度。10D130是一个半矮杆新品系, 用10D130和常规优良品种中双11杂交, 构建6世代遗传群体(P1、F1、P2、B1、B2和F2), 以主基因+多基因混合遗传模型对该组合株高及其关联性状进行遗传分析。结果表明, 10D130×中双11组合株高、分枝部位、主花序长度的遗传均受到1对加性-显性-上位性主基因+加性-显性-上位性多基因控制(D-0模型)。其中, 株高性状加性效应值为–8.58, 显性效应值为7.44, 主基因遗传率在B1、B2和F2中分别为23.52%、0.91%和17.81%；一次有效分枝起始部位的1对主基因加性效应值为–22.11, 显性效应值为3.13, 主基因遗传率在B1、B2和F2中分别为49.95%、40.85%和61.15%；主花序长的主基因加性效应值为–2.21, 显性效应值为1.6, 主基因遗传率在B1、B2和F2中分别为0.68%、47.94%和40.07%。一次有效分枝间距的最适宜遗传模型为E-1模型(2对加-显-上位性主基因+加-显-上位性多基因混合遗传模型), 其中第1对主基因加性效应值为–0.55、显性效应值为–1.66, 第2对主基因加性效应值为0.74、显性效应值为–1.29, 均表现超显性遗传, 主基因遗传率在B1、B2和F2三个分离世代群体中分别为10.99%、38.65%和44.10%。一次有效分枝部位高度、主花序长、有效分枝节间距和有效分枝数与株高均呈显著正相关。

关键词: 油菜, 株型, 主基因+多基因, 遗传分析

Abstract:

Improvement of plant types is one of main methods to increase yield and qualities of rapeseed. Dwarf and semi-dwarf plant types help for enhancements of lodging resistance and harvest index. Six generations (P1, P2, F1, F2, B1, and B2) were produced from a cross between semi-dwarf 10D130 and Zhongshuang 11 (ZS11) to analyze inheritances of plant height, branch height, length of main inflorescence, valid branch space and numbers of valid branch in rapeseed (Brassica napus) by the mixed major gene plus polygene inheritance model. The results showed that the plant height, branch height, length of main inflorescence and valid branch space of 10D130×ZS11 were dominated by a pair of major gene with additive-dominant-epistasis effects plus polygenes with additive-dominance-epistasis effects (D-0 model). The additive effect and dominant effect of major gene of plant height were –8.58 and 7.44, and the heritability of the major gene in B1, B2, and F2 was 23.52%, 0.91%, and17.81%, respectively. The additive effect and dominant effect of major gene of branch height were –22.11 and 3.13, and the heritability of the major gene in B1, B2 and F2 was 49.95%, 40.85%, and 61.15%, respectively. The additive effect and dominant effect of major gene of length of main inflorescence were –2.21 and 1.6, and the heritability of the major gene of length of main inflorescence in B1, B2, and F2 was 0.68%, 47.94%, and 40.07%. The first valid branch space of 10D130×ZS11 was dominated by two pairs of major gene with additive-dominant-epistasis effects plus polygenes with additive-dominance-epistasis (E-1 model). The additive effects of major genes are –0.55 and 0.74, and the dominance effects of the two major genes were –1.66 and –1.29, respectively, and the heritability of the major genes in B1, B2,and F2 was 10.99%, 38.65%, and 44.10%. The branch height, length of main inflorescence, valid branch space and number of valid branch were significantly and positively correlated with plant height.

Key words: Rapeseed, Plant type, Major gene plus polygene, Genetic analysis

[1]Hedden P. The genes of the green revolution. Trends Genet, 2003, 19: 5–9



[2]Foisset N, Delourme R, Barret P, Renard M. Molecular tagging of the dwarf BREIZH (Bzh) gene in Brassica napus. Theor Appl Genet, 1995, 91: 756–761



[3]Islam N, Evans E J. Influence of lodging and nitrogen rate on the yield and yield attributes of oilseed rape ( Brassica napus L.). Theor Appi Genet, 1994, 88: 530–534



[4]Zhou X-B(周晓彬), Xiao S-S(肖数数), Wang Y-Y(王莹莹), Feng X-X(冯星星), Wang D-P(王德鹏), Tang H-Y(唐浩月), Fan Y-G(范玉刚), Gong D-P(龚德平). Research progress of rapeseed lodging. Hubei Agric Sci (湖北农业科学), 2011, 50(20): 4105–4108 (in Chinese with English abstract)



[5]Khush G S. Green revolution: the way forward. Nat Rev Genet, 2001, 2: 815–822



[6]Muangprom A, Osborn T C. Characterization of a dwarf gene in Brassica rapa, including the identification of a candidate gene. Theor Appi Genet, 2004, 108: 1378–1384



[7]Mei D-S(梅德圣), Wang H-Z(王汉中), Li Y-C(李云昌), Hu Q(胡琼), Li Y-D(李英德), Xu Y-S(徐育松). The discovery and genetic analysis of dwarf mutation 99CDAM in Brassica napus L. Hereditas (遗传), 2006, 28(7): 851–857 (in Chinese with English abstract)



[8]Wang M L, Zhao Y, Chen F, Yin X C. Inheritance and potentials of a mutated dwarfing gene ndf1 in Brassica napus. Plant Breed, 2004, 123: 449–453



[9]Pu H-M(浦惠明), Qi C-K(戚存扣), Fu S-Z(傅寿仲). Preliminary study and identification of dwarf resource Aiyuan 1 in Brassica napus L. Crop Genet Resour (作物品种资源), 1995, (1): 23–24 (in Chinese with English abstract)



[10]Shi S-W(石淑稳), Wu J-S(吴江生), Liu H-L(刘后利). The siliques and dwarf mutant of rapeseed induced in vitro. Acta Agric Nucl Sin (核农学报), 1995, 9(4): 252–253 (in Chinese with English abstract)



[11]Huang T-D(黄天带), Wu J-S(吴江生), Wang L-Q(王令强), Hua Y-W(华玉伟). Genetic analysis on a dwarf Brassica napus mutant and screening RAPD markers linked to the dwarf gene. J Agric Biotechnol (农业生物技术学报), 2006, 14(6): 942–945 (in Chinese with English abstract)



[12]Zhou Q-Y(周清元), Li J-N(李加纳), Cui C(崔翠), Yin J-M(殷家明), Chen L(谌利), Tang Z-L(唐章林). Obtaining and character of the interspecfic hybrids between B. juncea and B. oleracea var. aceaphala. Acta Agron Sin (作物学报), 2005, 31(8): 1058–1063 (in Chinese with English abstract)



[13]Liu H-B(刘宏波), Liu Z-X(刘忠松), Guan C-Y(官春云), Chen S-Y(陈社员), Liu X-J(刘显军), Yang Y-Y(杨晔宇). The phenotypic identification in the rapeseed introgression lines derived from the interspecific cross of Brassica juncea×Brassica napus. J Hunan Agric Univ (Nat Sci) (湖南农业大学学报•自然科学版), 2007, 33(51): 156–161 (in Chinese)



[14]Liu S-Y(刘淑艳), Liu H-B(刘宏波), Liu Z-S(刘忠松), Guan C-Y(官春云). Breeding and studied of dwarf and resistant lodging newly germplasm in rapeseed. Hunan Agric Sci (湖南农业科学), 2007, (5): 42–43 (in Chinese with English abstract)



[15]Gai J-Y(盖钧镒), Zhang Y-M(章元明), Wang J-K(王建康). Genetic system of quantitative traits in plants (植物数量遗传体系). Beijing: Science Press, 2003. pp 96−168, 224-265, 322–323 (in Chinese)



[16]Zhang Y-M(章元明), Gai J-Y(盖钧镒), Wang J-K(王建康). Identification of major gene and polygene mixed inheritancemodel and estimation of genetic parameters of a quantitative trait from B1 and B2 or F2 progeny. J Biomathematics (生物数学学报), 2000, 15(3): 358−366 (in Chinese with English abstract)



[17]Zhang Y-M(章元明), Gai J-Y(盖钧镒). The IECM algorithm for estimation of component distribution parameters in segregating analysis of quantitative traits. Acta Agron Sin (作物学报), 2000, 26(6): 699−705 (in Chinese with English abstract)



[18]Dong F-M(董凤鸣), Zhou P(周萍). The application of excel in linear regression analysis. Sci Inform (科技信息), 2007, 12: 144–146 (in Chinese with English abstract)



[19]Wang C-W(王春娥), Gai J-Y(盖钧镒), Fu S-X(傅三雄), Yu D-Y(喻德跃), Chen S-Y(陈受宜). Inheritance and QTL mapping of tofu and soymilk output in soybean. Sci Agric Sin (中国农业科学), 2008, 41(5): 1274–1282 (in Chinese with English abstract)



[20]Gou H-Y(苟红英), Gao X(高雪). Influence of economic characters of Zaxuan 1 for N, P, K application mount. Gengzuo Yu Zaipai (耕作与栽培), 2003, (6): 30–32, 48(in Chinese)



[21]Xiao G- B(肖国滨), Ye C(叶川), Liu X-S(刘小三), Zheng W(郑伟), Huang T-B(黄天宝), Zhang K(张昆), Zhao M-Z(赵美珍). Effect of simplified fertilization on triple cropping rapeseed in red soil paddy field of Jiangxi. Acta Agric Jiangxi (江西农业学报), 2011, 23(5): 106–108 (in Chinese with English abstract)



[22]Huang Z-W(黄中文), Wang W(王伟) Xu X-J(徐新娟), Wen Z-X(文自翔), Li H-C(李海朝), Li J-Y(李金英), Lu W-G(卢卫国). Relationship of dynamic plant height and its relative growth rate with yield using recombinant inbred lines of soybean. Acta Agron Sin (作物学报), 2011, 37(3): 559−562 (in Chinese with English abstract)
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