欢迎访问作物学报,今天是

作物学报 ›› 2013, Vol. 39 ›› Issue (06): 1096-1104.doi: 10.3724/SP.J.1006.2013.01096

• 耕作栽培·生理生化 • 上一篇    下一篇

外源ABA和6-BA对不同持绿型小麦旗叶衰老的影响及其生理机制

杨东清,王振林*,尹燕枰,倪英丽,杨卫兵,蔡铁,彭佃亮,徐彩龙,崔正勇,刘铁宁,徐海成   

  1. 山东农业大学农学院 / 作物生物学国家重点实验室,山东泰安 271018
  • 收稿日期:2012-11-25 修回日期:2013-01-15 出版日期:2013-06-12 网络出版日期:2013-03-22
  • 通讯作者: 王振林, E-mail: zlwang@sdau.edu.cn, Tel: 0538-8241359
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项经费项目(201203100, 201203029),国家自然科学基金项目(31271661)和国家重点基础研究发展计划(973计划)项目(2009CB118602)资助。

Effects of Exogenous ABA and 6-BA on Flag leaf Senescence in Different Types of Stay-Green Wheat and Relevant Physiological Mechanisms

YANG Dong-Qing,WANG Zhen-Lin*,YIN Yan-Ping,NI Ying-Li,YANG Wei-Bing,CAI Tie,PENG Dian-Liang,XU Cai-Long,CUI Zheng-Yong,LIU Tie-Ning,XU Hai-Cheng   

  1. Agronomy College, Shandong Agricultural University / State Key Laboratory of Crop Biology, Tai’an 271018, China
  • Received:2012-11-25 Revised:2013-01-15 Published:2013-06-12 Published online:2013-03-22
  • Contact: 王振林, E-mail: zlwang@sdau.edu.cn, Tel: 0538-8241359

摘要:

为深入认识植物激素在小麦抗氧化和调控衰老中的作用机制,以持绿品种汶农6号和非持绿品种济麦20为材料,在盛花期后喷施脱落酸(ABA)6-苄基腺嘌呤(6-BA),考察外源激素对旗叶衰老过程中生理生化指标动态变化以及籽粒产量的影响,并探讨了激素与衰老的关系。结果表明,汶农6号旗叶的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性、叶绿素含量及籽粒千粒重和产量均大于济麦20,且丙二醛(MDA)含量低于济麦20,表明持绿型品种抗氧化能力强,衰老进程较慢,进而获得较高产量。外源ABA6-BA处理显著提高花后7~28 d旗叶叶绿素含量,提高花后21~35 d可溶性蛋白含量,显著降低花后28~35 d旗叶MDA含量。外源ABA降低两品种旗叶玉米素(ZR)含量,但提高生长素(IAA)和赤霉素(GA)含量,降低了其在花后28~35 dABA含量。6-BA处理提高旗叶ZR含量及花后7~14 d IAA含量,降低济麦20花后21~35 d旗叶ABA含量。外源ABA显著提高汶农6号花后7~21 d旗叶SOD活性,喷施ABA对汶农6号旗叶POD和过氧化氢酶(CAT)活性影响没有显著性影响,但显著提高了济麦20花后7~28 d POD活性,喷施6-BA提高了两品种7~28 d SODPODCAT活性。总之,ABA6-BA处理改变了旗叶内源激素水平,提高抗氧化酶活性,降低MDA含量,延缓旗叶衰老,从而提高了籽粒产量。

关键词: 小麦, 持绿, 激素, 抗氧化酶, 产量

Abstract:

The effects of exogenous abscisic acid (ABA) and 6-benzyladenine (6-BA) on the contents of chlorophyll, malondialdehyde (MDA), and endogenous hormones and activities of antioxidant enzymes in flag leaf were investigated using stay-green wheat cultivar Wennong 6 and a control cultivar Jimai 20. The results showed that ABA and 6-BA obviously increased chlorophyll content from seven days after anthesis (DAA) to twenty-eight DAA. Likewise, the soluble protein contents in ABA-treated and 6-BA-treated plants significantly increased from 21 DAA to 35 DAA, while the MDA content decreased from 28 DAA to 35 DAA. Although ABA applications decreased endogenous zeatin (ZR) and ABA contents from 28 DAA to 35 DAA, the endogenous auxin (IAA) and gibberellin (GA) contents increased. In both cultivars, 6-BA significantly increased the ZR content, but decreased the ABA content in Jimai 20 from 21 DAA to 35 DAA. Exogenous ABA and 6-BA significantly enhanced superoxide dismutase (SOD) activity, while had no significant effects on peroxidase (POD) and catalase (CAT) activities in Wennong 6 and increased POD activity in Jimai 20 from 7 DAA to 28 DAA. Exogenous 6-BA significantly increased POD and CAT activities in both cultivars. Meanwhile, treatments with ABA and 6-BA significantly increased 1000-grain weight and yield, that had no effects on grain number per spike and spike number per square meter.

Key words: Wheat, Stay-green, Hormones, Antioxidant enzymes, Yield

[1]Yan C-S(阎成士), Li D-Q(李德全), Zhang J-H(张建华). Plant leaf senescence and oxidative stress. Chin Bull Bot (植物学通报), 1999, 16(4): 398–404 (in Chinese with English abstract)



[2]Li H(李红), Tang M-F(唐明凤), Yu F(喻凤), Luo P-G(罗培高). Determination the influence of removing flag leaf during different stages afer heading on wheat yield. Acta Agric Boreali-Sin (华北农学报), 2010, 25(12): 93–97 (in Chinese with English abstract)



[3]Duan L-S(段留生), Han B-W(韩碧文), He Z-P(何钟佩). The effects of correlation between leaf and other organs on leaf senescence. Chin Bull Bot (植物学通报), 1998, 15(1): 43–49 (in Chinese with English abstract)



[4]Xu Z-H(许智宏), Li J-Y(李家洋). Plant hormones research in China: past, present and future. Chin Bull Bot (植物学通报), 2006, 23(5): 433–442 (in Chinese with English abstract)



[5]Guo W-Q(郭文琦), Chen B-L(陈兵林), Liu R-X(刘瑞显), Zhou Z-G(周治国). Effect of nitrogen application rate on cotton leaf antioxidant enzyme activities and endogenous hormone contents under short-term waterlogging at flowering and boll-forming stage. Chin J Appl Ecol(应用生态学报), 2010, 21(1): 53–60 (in Chinese with English abstract)



[6]Wu Y-R(吴耀荣), Xie Q(谢旗). ABA and plant stress response. Chin Bull Bot (植物学通报), 2006, 23(5): 511–518 (in Chinese with English abstract)



[7]Aroca R, Vernieri P, Irigoyen J J, Sanchez M, Tognoni F, Pardossi A. Involvement of abscisic acid in leaf and root of maize (Zea mays L.) in avoiding chilling-induced water stress. Plant Sci, 2003, 165: 671–679



[8]Zhu Z-H(朱中华), Duan L-S(段留生), Feng X-M(冯雪梅), Han B-W(韩碧文), He Z-P(何钟佩). Systemmatical analysis of hormones regulation on wheat leaves senescence. Acta Agron Sin (作物学报), 1998, 24(2): 176–181 (in Chinese with English abstract)



[9]Hu X L, Jiang M Y, Zhang A Y, Lu J. Abscisic acid-induced apoplastic H2O2 accumulation up-regulates the activities of chloroplastic and cytosolic antioxidant enzymes in maize leaves. Planta, 2005, 223: 57–68



[10]Huang F-L(黄凤莲), Dai L-Y(戴良英), Luo K(罗宽). Mechanism studies on pharmaceutical induction for resistance to cold of rice seedlings. Acta Agron Sin (作物学报), 2000, 26(1): 92–97 (in Chinese with English abstract)



[11]Travaglia C, Reinoso H, Cohen A, Luna C, Tommasino E, Castillo C, Bottini R. Exogenous ABA increases yield in field-grown wheat with moderate water restriction. J Plant Growth Regul, 2010, 29: 366–374



[12]Lin D-B(林定波), Liu Z-Q(刘祖棋). Effect of cold acclimation and ABA on membrane stability and synthesis of membrane protein in citrus. J Nanjing Agric Univ (南京农业大学学报), 1994, 17(1): 1–5 (in Chinese with English abstract)



[13]Jiang H(姜华), Wan J(万佳), Gao X-L(高晓玲), Chen J(陈静), Wang X-D(王旭东), Xu Z-J(徐正军). Purification and analysis of abscisic acid-specifically-inducible protein from rice callus. Chin J Rice Sci (中国水稻科学), 2006, 20(6): 583–588 (in Chinese with English abstract)



[14]Zhang H-N(张海娜), Li C-D(李存东), Xiao K(肖凯). Regulation effects of exogenous 6-BA on photosynthesis and leaf senescence in cotton. Cott Sci (棉花学报), 2007, 19(6): 467–471 (in Chinese with English abstract)



[15]Liu X, Huang B. Cytokinin effects on creeping bentgrass response to heat stress: II. Leaf senescence and antioxidant metabolism. Crop Sci, 2002, 42:466–473



[16]Shao R-X(邵瑞鑫), Li J(李建), Xin L-F(信龙飞), Zhao Y(赵宇), Yang Q-H(杨青华). Effects of kinetin and succinic acid seed dressing on the ultrastructure of chloroplasts and chlorophyll fluorescence parameters in aging process of maize. Plant Nutr Fert Sci (植物营养与肥料学报), 2012, 18(6): 1362–1369 (in Chinese with English abstract)



[17]Wang Y-R(王彦荣), Hua Z-T(华泽田), Chen W-F(陈温福), Dai G-J(代贵金), Hao X-B(郝宪彬), Wang Y(王岩), Zhang Z-X(张忠旭), Sui G-M(隋国民). Relation between root and leaf senescence and their effects on grain-filling in japonica rice. Acta Agron Sin (作物学报), 2003, 29(6): 892–898 (in Chinese with English abstract)



[18]Thomas H, Howarth C J. Five ways to stay green. J Exp Bot, 2000, 51: 329–337



[19]Liu Y(刘洋), Shi H-Q(石慧清), Gong Y-H(龚月桦). Effects of sulfur and nitrogen fertilizer combination on nitrogen transfer and leaf senescence in stay-green wheat. Acta Acta Bot Boreali-Occident Sin (西北植物学报), 2012, 32(6): 1206–1213 (in Chinese with English abstract)



[20]Luo P-G(罗培高), Ren Z-L(任正隆), Wu X-H(吴先华), Zhang H-Y(张怀渝), Zhang H-Q(张怀琼), Feng J(冯娟). The structure and physiological and biochemical mechanism of delaying senescence of wheat. Chin Sci Bull (科学通报), 2006, 51(18): 2154–2460 (in Chinese)



[21]Zhao S-J(赵世杰), Shi G-A(史国安), Dong X-C(董新纯). Experimental Guide for Plant Physiology (植物生理实验指导). Beijing: China Agricultural Science and Technology Press, 1998. pp 55–60 (in Chinese)



[22]Read S M, Northeote D H. Minimization of variation in the response to different protein of the coomassic blue G dye-binding assay for protein. Anal Biochem, 1981, 116: 53–64



[23]Zhao S-J(赵世杰), Xu C-C(许长城), Zou Q(邹琦), Meng Q-W(孟庆伟). The improvement of MDA measurement in plant. Plant Physiol Commun (植物生理学通讯), 1994, 30(3): 207–210 (in Chinese)



[24]Yang W-B(杨卫兵), Wang Z-L(王振林), Yin Y-P(尹燕枰), Li W-Y(李文阳), Li Y(李勇), Chen X-G(陈晓光), Wang P(王平), Chen E-Y(陈二影), Guo J-X(郭俊祥), Cai T(蔡铁), Ni Y-L(倪英丽). Effects of spraying exogenous ABA or GA on the endogenous hormones concentration and filling of wheat grains. Sci Agric Sin (中国农业科学), 2011, 44(13): 2673–2682 (in Chinese with English abstract)



[25]Zou Q(邹琦). Experimental of Plant Physiology (植物生理学实验指导). Beijing: China Agriculture Press, 2000 (in Chinese)



[26]Xue H(薛晖), Jia L(贾丽), Gong Y-H(龚月桦), Liu Y-Z(刘赢洲), Wu Y-H(吴养会). Study on the stay-green capacity and leaf senescence of winter wheat. Acta Bot Boreali-Occident Sin (西北植物学报), 2010, 30(2): 336–343 (in Chinese with English abstract)



[27]Liu L-T(刘连涛), Li C-D(李存东), Sun H-C(孙红春), Zhang Y-J(张永江), Bai Z-Y(白志英), Feng L-X(冯丽肖). Effects of nitrogen on cotton senescence and the corresponding physiological mechanisms. Sci Agric Sin (中国农业科学), 2009, 42(5): 1575–1581 (in Chinese with English abstract)



[28]Wang Y-X(汪月霞), Suo B(索标), Zhao P-F(赵鹏飞), Qu X-F(取小菲), Yuan L-G(袁利刚), Zhao X-J(赵雪娟), Zhao H-J(赵会杰). Effect of abscisic acid treatment on psbA gene expression in two wheat cultivars during grain filling stage under drought stress. Acta Agron Sin (作物学报), 2011, 37(8): 1372–1377 (in Chinese with English abstract)



[29]Sun Z-Y(孙振元), Duan L-S(段留生), Han B-W(韩碧文), Liu S-L(刘淑兰). Stereological changes of chloroplast ulteastructure and the effects of 6-benzylaminopurine during wheat leaf senescence. Acta Agron Sin (作物学报), 1998, 24(6): 818–820 (in Chinese with English abstract)



[30]Wang Y-F(王月福), Yu Z-W(于振文), Li S-X(李尚霞), Yu S-L(余松烈). Effect of nitrogen nutrition on protein and nucleic acid metabolism during senescence of flag leaf. Plant Nutr Fert Sci (植物营养与肥料学报), 2003, 9(2): 178–183 (in Chinese with English abstract)



[31]Yu H(鱼欢), Feng B-L(冯佰利), Zhang Y(张英), Liu P-T(刘鹏涛), He Y-Y(何永艳), Dai H-P(代惠萍), Li S-X(李生秀). Leaf aging and reactive oxygen species metabolism of winter wheat in different cultivation modes. Acta Agron Sin (作物学报), 2007, 33(10): 1729–1732 (in Chinese with English abstract)



[32]Zhang W-W(张微微), Huang X-L(黄晓林), Zhao X(赵霞), Chen S-B(陈胜兵), Rong L(荣利), Chen X(陈旭), Tang Y-Z(汤一卒). Research progress on enzymatic antioxidant system response to stress in cotton. Chin Agric Sci Bull (中国农学通报), 2009, 25(19): 108–112 (in Chinese with English abstract)



[33]Wang H(王虹), Jiang Y-P(姜玉萍), Shi K(师恺), Zhou Y-H(周艳虹), Yu J-Q(喻景权). Effects of light quality on leaf senescence and activities of antioxidant enzymes in cucumber plants. Sci Agric Sin (中国农业科学), 2010, 43(3): 529–534 (in Chinese with English abstract)



[34]Zhu D(朱丹), Jiang M-Y(蒋明义), Tan M-P(谭明普). The mechanism of ABA-induced apoplastic H2O2 accumulation in maize leaves. J Plant Physiol Mole Biol (植物生理与分子生物学学报), 2006, 32(5): 519–526 (in Chinese with English abstract)



[35]Xu S-C(许树成), Ding H-D(丁海东), Lu R(鲁锐), Shi B(石犇), Ma F-F(马芳芳). Study on effects of ABA in antioxidant defense of plant cells. J China Agric Univ (中国农业大学学报), 2008, 13(2): 11–19 (in Chinese with English abstract)



[36]Hironori K, Shigeto M, Megumi T, Takehiro M, Kunisuke T. Differential gene expressions of rice superoxide dismutase isoforms to oxidative and environmental stresses. Free Radical Res, 1999, 31: 219–255



[37]Xu S C, Ding H D, Su F X, Zhang A Y, Jiang M Y. Involvement of protein phosphorylation in water stress-induced antioxidant defense in maize leaves. J Integr Plant Biol, 2009, 51: 654–662



[38]Zhang H-N(张海娜), Li C-D(李存东), Xiao K(肖凯). The physiological mechanism of plant growth and senescence characteristics in wheat regulated by exogenous cytokinin. Acta Agric Boreali-Sin (华北农学报), 2007, 22(5): 1–4 (in Chinese with English abstract)



[39]Wang S-G(王三根). Roles of cytokinin on stress-resistance and delaying senescence in plants. Chin Bull Bot (植物学通报), 2000, 17(2): 121–126 (in Chinese with English abstract)



[40]He P(何萍), Jin J-Y(金继云). Relationships among hormone changes, transmembrane Ca2+ flux and lipid peroxidation during leaf senescence in spring maize. Acta Bot Sin (植物学报), 1999, 41(11): 1221–1225 (in Chinese with English abstract)



[41]Zheng S-S(郑莎莎), Sun C-F(孙传范), Sun H-C(孙红春), Liu L-T(刘连涛), Zhao J-F(赵金峰), Li C-D(李存东). Effects of different exogenous hormones on physiological characteristics of main stem leaves at flower and boll stage in cotton. Sci Agric Sin (中国农业科学), 2009, 42(12): 4383–4389 (in Chinese with English abstract)



[42]Xiao K(肖凯), Zhang R-X(张荣铣), Qian W-P(钱维朴). The physiological mechanism of senescence and photosynthetic function decline of flag leaf in wheat regulated by nitrogen nutrition. Plant Nutr Fert Sci (植物营养与肥料学报), 1998, 4(4): 371–378 (in Chinese with English abstract)



[43]Bao F(包方), Hu Y-X(胡玉欣), Li J-Y(李家洋). Identification of auxin responsive genes in arabidopsls by cDNA array. Chin Sci Bull (科学通报), 2001, 46(23): 1988–1992 (in Chinese)



[44]Wang D(王丹), Fan S-L(范术丽), Song M Z(宋美珍), Pang Z-Y(庞朝友), Yu S-X(喻树迅). The effect of gibberellm on leaf senescenle-associated genes. China Cott (中国棉花), 2011, 38(3): 11–13 (in Chinese with English abstract)



[45]Huang S(黄森), Zhang J-S(张继澍), Zhang Y-M(张院民). Effect of GA3 treatment on ethylene biosynthesis in post-harvested persimmon fruits. Chin Agric Sci Bull (中国农学通报), 2006, 22(2): 88–90 (in Chinese with English abstract)



[46]Wang Z(王忠). Plant Physiology (植物生理学). Beijing: China Agriculture Press, 2006. pp 424–425 (in Chinese)



[47]Wang X-Y(王晓云), Zou Q(邹琦). Advances in studies on relationship between polyamines and plant senescence. Chin Bull Bot (植物学通报), 2002, 19(1): 11–20 (in Chinese with English abstract)



[48]Zheng D-F(郑殿峰), Zhao L-M(赵黎明), Feng N-J(冯乃杰). Effects of plant growth regulators (PGRs) on endogenous hormone contents and activities of protective enzymes in soybean leaves. Acta Agron Sin (作物学报), 2008, 34(7): 1233–1239 (in Chinese with English abstract)

[1] 胡文静, 李东升, 裔新, 张春梅, 张勇. 小麦穗部性状和株高的QTL定位及育种标记开发和验证[J]. 作物学报, 2022, 48(6): 1346-1356.
[2] 王丹, 周宝元, 马玮, 葛均筑, 丁在松, 李从锋, 赵明. 长江中游双季玉米种植模式周年气候资源分配与利用特征[J]. 作物学报, 2022, 48(6): 1437-1450.
[3] 王旺年, 葛均筑, 杨海昌, 阴法庭, 黄太利, 蒯婕, 王晶, 汪波, 周广生, 傅廷栋. 大田作物在不同盐碱地的饲料价值评价[J]. 作物学报, 2022, 48(6): 1451-1462.
[4] 颜佳倩, 顾逸彪, 薛张逸, 周天阳, 葛芊芊, 张耗, 刘立军, 王志琴, 顾骏飞, 杨建昌, 周振玲, 徐大勇. 耐盐性不同水稻品种对盐胁迫的响应差异及其机制[J]. 作物学报, 2022, 48(6): 1463-1475.
[5] 杨欢, 周颖, 陈平, 杜青, 郑本川, 蒲甜, 温晶, 杨文钰, 雍太文. 玉米-豆科作物带状间套作对养分吸收利用及产量优势的影响[J]. 作物学报, 2022, 48(6): 1476-1487.
[6] 陈静, 任佰朝, 赵斌, 刘鹏, 张吉旺. 叶面喷施甜菜碱对不同播期夏玉米产量形成及抗氧化能力的调控[J]. 作物学报, 2022, 48(6): 1502-1515.
[7] 李祎君, 吕厚荃. 气候变化背景下农业气象灾害对东北地区春玉米产量影响[J]. 作物学报, 2022, 48(6): 1537-1545.
[8] 郭星宇, 刘朋召, 王瑞, 王小利, 李军. 旱地冬小麦产量、氮肥利用率及土壤氮素平衡对降水年型与施氮量的响应[J]. 作物学报, 2022, 48(5): 1262-1272.
[9] 石艳艳, 马志花, 吴春花, 周永瑾, 李荣. 垄作沟覆地膜对旱地马铃薯光合特性及产量形成的影响[J]. 作物学报, 2022, 48(5): 1288-1297.
[10] 闫晓宇, 郭文君, 秦都林, 王双磊, 聂军军, 赵娜, 祁杰, 宋宪亮, 毛丽丽, 孙学振. 滨海盐碱地棉花秸秆还田和深松对棉花干物质积累、养分吸收及产量的影响[J]. 作物学报, 2022, 48(5): 1235-1247.
[11] 柯健, 陈婷婷, 吴周, 朱铁忠, 孙杰, 何海兵, 尤翠翠, 朱德泉, 武立权. 沿江双季稻北缘区晚稻适宜品种类型及高产群体特征[J]. 作物学报, 2022, 48(4): 1005-1016.
[12] 李瑞东, 尹阳阳, 宋雯雯, 武婷婷, 孙石, 韩天富, 徐彩龙, 吴存祥, 胡水秀. 增密对不同分枝类型大豆品种同化物积累和产量的影响[J]. 作物学报, 2022, 48(4): 942-951.
[13] 王吕, 崔月贞, 吴玉红, 郝兴顺, 张春辉, 王俊义, 刘怡欣, 李小刚, 秦宇航. 绿肥稻秆协同还田下氮肥减量的增产和培肥短期效应[J]. 作物学报, 2022, 48(4): 952-961.
[14] 杜浩, 程玉汉, 李泰, 侯智红, 黎永力, 南海洋, 董利东, 刘宝辉, 程群. 利用Ln位点进行分子设计提高大豆单荚粒数[J]. 作物学报, 2022, 48(3): 565-571.
[15] 付美玉, 熊宏春, 周春云, 郭会君, 谢永盾, 赵林姝, 古佳玉, 赵世荣, 丁玉萍, 徐延浩, 刘录祥. 小麦矮秆突变体je0098的遗传分析与其矮秆基因定位[J]. 作物学报, 2022, 48(3): 580-589.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!