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作物学报 ›› 2013, Vol. 39 ›› Issue (07): 1248-1256.doi: 10.3724/SP.J.1006.2013.01248

• 耕作栽培·生理生化 • 上一篇    下一篇

大豆苗期耐盐性的简便鉴定方法

姜静涵,郭勇,常汝镇,关荣霞*,邱丽娟*   

  1. 中国农业科学院作物科学研究所 / 农作物基因资源与遗传改良国家重大科学工程 / 农业部北京大豆生物学重点实验室，北京100081
  • 收稿日期:2012-12-27 修回日期:2013-03-11 出版日期:2013-07-12 网络出版日期:2013-04-23
  • 通讯作者: 邱丽娟, E-mail: qiu_lijuan@263.net, Tel: 010-62135623; 关荣霞, E-mail: rx_guan@sina.com
  • 基金资助:

    本研究由国家自然科学基金项目(30971801)，国家转基因生物新品种培育重大专项(2009ZX08009-088B, 2011ZX08004-002)和国家重点基础研究发展计划(973计划)项目(2009CB118400)资助。

Simple Evaluation Method of Tolerance to Salt at Seedling Stage in Soybean

JIANG Jing-Han,GUO Yong,CHANG Ru-Zhen,GUAN Rong-Xia*,QIU Li-Juan*   

  1. National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Soybean Biology, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2012-12-27 Revised:2013-03-11 Published:2013-07-12 Published online:2013-04-23
  • Contact: 邱丽娟, E-mail: qiu_lijuan@263.net, Tel: 010-62135623; 关荣霞, E-mail: rx_guan@sina.com

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26

摘要：

盐胁迫是影响大豆产量的重要非生物胁迫因素，筛选耐盐种质资源对培育耐盐大豆品种具有重要的意义。本研究利用4个耐盐品种和4个盐敏感品种进行苗期耐盐性鉴定，以蛭石为培养基质，在大豆真叶展开时，分别用100、200和250 mmol L–1的NaCl溶液处理，每2 d浇一次盐溶液，每天测定大豆真叶SPAD值。盐处理8 d后，分别取各品种的根、茎、叶，用原子吸收光谱仪测定其Na+含量。结果表明，用200 mmol L–1 NaCl处理的耐盐品种和盐敏感品种表型差异明显，但叶片Na+含量差异不显著；用250 mmol L–1 NaCl处理后，表型差异明显且叶片Na+含量差异显著。200 mmol L–1和250 mmol L–1 NaCl处理下叶片失绿明显，盐敏感品种叶片积累的Na+含量与SPAD值极显著负相关。本研究建立了一种以蛭石为基质，用200 mmol L–1或250 mmol L–1 NaCl处理，8 d即可进行大豆苗期耐盐性鉴定的简便方法，为大豆种质资源苗期耐盐性的大规模鉴定及耐盐品种选择和耐盐机理的研究提供了方法。

关键词: 大豆, 苗期, 耐盐性, Na+

Abstract:

Salt stress seriously affected the production of soybean. Thus screening salt-tolerant soybeans is very important for developing salt resistant cultivars. The objective of this study was to establish a simple method for screening soybean varieties in response to salt stress. The seedlings of four salt-tolerant varieties and four salt-sensitive varieties were grown in vermiculite pouring with 0, 100, 200, and 250 mmol L–1 NaCl solutions, adding each two days for three times. The SPAD was measured every day. The root, stem and leaf were sampled after eight days of treatment and Na+ content were measured by the atomic absorption spectrophotometer. The results indicated that it is easy to distinguish the level of salt tolerance based on foliar symptoms in both treatment of 200 mmol L–1 and 250 mmol L–1 NaCl. Under 250 mmol L–1 NaCl stress, the Na+ content in leaf for salt-tolerant varieties was lower than that for salt-sensitive varieties. There was a significant correlation between Na+ content and SPAD in salt-sensitive varieties. This experiment established a convenient method of soybean salinity tolerance identification at seedling stage, using the seedlings grown in vermiculite with 200 mmol L–1 and 250 mmol L–1 NaCl treatment for eight days. It will facilitate the screening of germplasm, mechanism study and breeding selection on a large scale.

Key words: Soybean, Seedling stage, Salt tolerance, Na+

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