作物学报 ›› 2013, Vol. 39 ›› Issue (08): 1345-1351.doi: 10.3724/SP.J.1006.2013.01345
彭学聪,杨秀芬,邱德文,曾洪梅,郭立华,刘峥*
PENG Xue-Cong,YANG Xiu-Fen,QIU De-Wen,ZENG Hong-Mei,GUO Li-Hua,LIU Zheng*
摘要:
Hrip1是从极细链格孢(Alternaria tenuissima)代谢物中分离的一种蛋白激发子。将蛋白激发子基因Hrip1转化到拟南芥,对5个T1代转基因拟南芥株系进行分子检测, 证明Hrip1基因能够在拟南芥中转录和表达。转基因植株对盐和干旱胁迫的抗性显著增强, 75 mmol L−1 NaCl和50 mmol L−1甘露醇渗透胁迫2 d, 转基因植株种子平均相对发芽率为32.1%和77.9%, 分别比野生型的增加3.72倍和5.61倍; 150 mmol L−1 NaCl和50 mmol L−1甘露醇处理拟南芥幼苗7 d后, 转基因植株平均相对根长为81.79%和93.25%, 分别是野生型的1.53倍和1.34倍。3周龄的转基因植株在250 mmol L−1 NaCl条件下胁迫20 d, 平均存活率为67%, 显著高于野生型(42%)(P<0.05); 干旱胁迫25 d后, 复水5 d转基因植株平均存活率为72%, 而野生型仅为44%。检测结果显示转基因植株叶片的抗氧化酶活性明显高于野生型, 用200 mmol L−1 NaCl和200 mmol L−1甘露醇处理24 h后, POD活性分别比野生型植株提高1.56倍和1.85倍, CAT活性分别比野生型植株提高1.64和1.86倍。说明蛋白激发子Hrip1基因在拟南芥中的表达能够改善和提高植株的耐盐抗旱能力。
[1]Xiong L Z, Yang Y. Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase. Plant Cell Online, 2003, 15: 745–759[2]Fujita M, Fujita Y, Noutoshi Y, Takahashi F, Narusaka Y, Shinozaki K Y, Shinozaki K. Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks. Curr Opin Plant Biol, 2006, 9: 436–442[3]Zhang Y H, Yang X F, Liu Q, Zhang Y L, Zeng H M, Yuan J J, Mao J J. Purification of novel protein elicitor from Botrytis cinerea that induces disease resistance and drought tolerance in plants. Microbiol Res, 2010, 165: 142–151[4]Zhao M-Z(赵明治), Yang X-F(杨秀芬), Zhang M(张明), Yuan J-J(袁京京), Qiu D-W(邱德文). Purification and Bioactivities of a Protein Growth-activator from Aternaria tenuissima. Chin J Biol Control (中国生物防治), 2007, 23(2): 170–173 (in Chinese with English abstract)[5]Yang Y Y, Zhang H J, Li G J, Li W, Wang X E, Song F M. Ectopic expression of MgSM1, a Cerato-platanin family protein from Magnaporthe grisea, confers broad-spectrum disease resistance in Arabidopsis. Plant Biotechnol J, 2009, 7(8): 763–777[6]Qiu D W, Mao J J, Yang X F, Zeng H M. Expression of an elicitor-encoding gene from Magnaporthe grisea enhances resistance against blast disease in transgenic rice. Plant Cell Rep, 2009, 28: 925–933[7]Kulye M, Liu H, Zhang Y L, Zeng H M, Yang X F, Qiu D W. Hrip1, a novel protein elicitor from necrotrophic fungus, Alternaria tenuissima, elicits cell death, expression of defence-related genes and systemic acquired resistance in tobacco. Plant Cell Environ, 2012, 35: 2104–2120[8]Clough S J, Bent A F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J, 1998, 16: 735–743[9]Kobayashi K, Cabral S, Calamante G, Maldonado S. Mentaberry A. Transgenic tobacco plants expressing the potato virus X open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus. Mol Plant-Microbe Interact, 2001, 14: 1274−1285[10]Dai X Y, Xu Y Y, Ma Q B, Xu W Y, Wang T, Xue Y B, Chong K. Overexpression of an R1R2R3 MYB gene, OsMYB3R-2, increases tolerance to freezing, drought, and salt stress in transgenic Arabidopsis. Plant Physiol, 2007, 143: 1739–1751[11]Liu Y-C(刘颖超). Resistance Mechanism and Pathogensis-related Genes ExPression in Different Arabidopsis thaliana Ecotypes to Phytophthora infestans. PhD Dissertation of Agricultural University of Hebei, 2004 (in Chinese with English abstract)[12]Zhang Y-H(张云华), Qiu D-W(邱德文), Zhang L-J(张立军), Yang X-F(杨秀芬), Zeng H-M(曾洪梅), Yuan J-J(袁京京). Purification and its bioactivity of an activator protein PEBC2 from Botrytis cinerea. Acta Phytophyl Sin (植物保护学报), 2009, 35(2): 123–126 (in Chinese with English abstract)[13]Tang H-K(唐宏琨), Zeng H-M(曾洪梅). Recent advances in fungal protein elicitor and its transgenic plants. Chin J Biol Control (中国生物防治), 2010, 26(4): 480–485 (in Chinese with English abstract)[14]Kong X P, Pan J W, Zhang M Y, Xing X, Zhou Y, Liu Y, Li D P, Li D Q. ZmMKK4, a novel group C mitogen‐activated protein kinase kinase in maize (Zea mays), confers salt and cold tolerance in transgenic Arabidopsis. Plant Cell Environ, 2011, 34: 1291–1303[15]Ali Q, Ashraf M. Induction of drought tolerance in maize (Zea mays L.) due to exogenous application of trehalose: growth, photosynthesis, water relations and oxidative defence mechanism. J Agron Crop Sci, 2011, 197: 258–271[16]Zhang Y M, Yang J F, Lu S Y, Cai J L, Guo Z F. Overexpressing SgNCED1 in tobacco increases ABA level, antioxidant enzyme activities, and stress tolerance. J Plant Growth Regul, 2008, 27: 151–158 |
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