高丹草遗传效应与杂种表现预测模型

doi:10.3724/SP.J.1006.2014.00466

摘要/Abstract

摘要：

以5个高粱不育系材料为母本, 18个优质苏丹草材料为父本, 按照遗传交配设计(NCII)配制成90个杂交组合。分别在内蒙呼市和包头两地, 利用与高丹草产量相关的QTL位点标记检测亲本间的遗传差异, 并将F₁的8个性状表型值对亲本材料进行标记位点的筛选, 建立标记效应和标记型值估算体系。估算特异性位点对性状表现的效应及杂种标记型值, 进而分析杂种标记型值与杂种表现的相关性, 应用逐步回归分析法建立8个性状杂种表现的预测模型, 并通过Jackknife抽样技术检测模型的精确度和稳定性。结果显示, 在分别考虑显性、加性作用下8个性状的标记型值与表型值的相关系数平均为0.65, 各性状的可决系数较大(0.51~0.88), 而且两地结果趋势一致, 表明该预测模型稳定性强, 精确度较高。该模型对高丹草的杂种表现预测以及亲本选配都具有一定的指导意义。

关键词: 高丹草, 遗传效应, AFLP, SSR, SRAP, 杂种表现, 预测模型

Abstract:

Molecular markers-based heterosis prediction can provide some advices for Sorghum ×Sudan grass breeding. The 90 cross combinations were made according to the North Carolina design II (NCII) with five sorghum sterile lines as maternal parents and 18 Sudan grass lines as paternal parents. The field trials were carried out on the farms in Hohhot and Baotou to evaluate environmental effects. The parental genetic differences were compared by yield related QTL markers. The phenotypic values of eight traits for all F₁ hybrids were investigated. The values were used for selecting significant marker loci on parental lines for setting up the evaluation system of marker effect and marker value. The specific loci were used for evaluating the trait effects and hybrid marker value, and for analyzing the correlation between the marker value and heterosis. The prediction models of the eight traits for the hybrid were constructed with the stepwise regression analysis. The Jackknife sampling method was used to test the accuracy and stability of the model. The result indicated that, considering dominance and additive effect separately, eight traits showed the average correlation coefficient of 0.65 between vigor value and phenotypic value. The coefficient of determination was from 0.51 to 0.88 in the eight traits. The results in two places were similar. The model could be instructive for heterosis prediction and parents selection.

Key words: Sorghum ×Sudan grassGenetic effect, AFLP, SSR, SRAP, Heterosis, Prediction model

逯晓萍,刘丹丹,王树彦,米福贵,韩平安,吕二锁. 高丹草遗传效应与杂种表现预测模型[J]. 作物学报, 2014, 40(03): 466-475.

LU Xiao-Ping,LIU Dan-Dan,WANG Shu-Yan,MI Fu-Gui,HAN Ping-An,Lü Er-Suo. Genetic Effects and Heterosis Prediction Model of Sorghum bicolor × S.sudanense Grass[J]. Acta Agron Sin, 2014, 40(03): 466-475.

参考文献

[1]詹秋文, 钱章强. 高粱与苏丹草杂种优势利用的研究. 作物学报, 2004, 30: 73–77

Zhan Q W, Qian Z Q. Research of Sorghum-Sudan grass of heterosis utilization. Acta Agron Sin, 2004, 30: 73–77 (in Chinese with English abstract)

[2]王和平, 张福耀, 程庆军, 平俊爱, 杜志宏, 郭玉兰, 王晋中. 高粱-苏丹草杂交草的研究与利用. 杂粮作物, 2000, 20(4): 20–23

Wang H P, Zhang F Y, Cheng Q J, Ping J A, Du Z H, Guo Y L, Wang J Z. The Research and utilization of Sorghum-Sudan grass. Cereal Crops, 2000, 20(4): 20–23 (in Chinese)

[3]赵甘霖, 丁国祥, 熊洪, 曾庆曦. 我国高粱-苏丹草杂交种研究进展及应用前景. 西南农业学报, 2003, 16(4): 126–129

Zhao G L, Ding G X, Xiong H, Zeng Q X. Prospect of progress and application of Sorghum-Sudan grass in China. Southwest China J Agric Sci, 2003, 16(4): 126–129 (in Chinese with English abstract)

[4]Chung J M, Lee B C, Kim J S. Fine-scale structure among genetic individuals of clone-forming monotypic genus Echinosophora koreensis (Fabaceae). Ann Bot, 2006, 98: 165–173

[5]钱章强. 利用高粱雄性不育系选育饲用新品种. 中国草地, 1990, (6): 61–71

Qian Z Q. Breeding of new variety with male sterile line of sorghum. China Grassland, 1990, (6): 61–71 (in Chinese with English abstract)

[6]牟芝兰. 刈割周期对苏丹草叶片生长的影响. 草业科学, 1994, 11(6): 29–31

Mou Z L. Research of foliage growth of sorghum-sudan grass after cutting. Pratac Sci, 1994, 11(6): 29–31 (in Chinese with English abstract)

[7]陈才夫, 王槐三. 苏丹草×拟高粱种间杂种主要特性及细胞学分析. 南京农业大学学报, 1993, (2): 58–63

Chen C F, Wang H S. Main characteristics and cytological analysis of Sudan grass × sorghum interspecific hybrids. J Nanjing Agric Univ, 1993, (2): 58–63 (in Chinese with English abstract)

[8]孟钢. "先锋"高丹草-饲用高粱和苏丹草杂交品种. 世界农业, 2003, 6(6): 46–48

Meng G. “Pioneer” sorghum-Sudan grass ― hybrid of forage sorghum and Sudan grass. World Agric, 2003, 6(6): 46–48 (in Chinese)

[9]于卓, 赵晓杰, 秦永梅. 高粱与苏丹草2个杂交组合F2代的生育及细胞遗传学研究. 草业科学, 2004, 7: 13–17

Yu Z, Zhao X J, Qin Y M. Growth and cyto-genetics of F2 generation in Sorghum × Sudan grass combinations. Pratac Sci, 2004, 7: 13–17 (in Chinese with English abstract)

[10]赵晓杰, 于卓, 刘永伟, 逯晓萍. 高粱314A、13A与苏丹草杂种F1代的农艺特性及细胞学分析. 西北植物学报, 2005, 25: 1107–1113

Zhao X J, Yu Z, Liu Y W, Lu X P. Agronomic trait and cytology of hybrid F1 of sorghum (314A and 13A) × Sudan grass. Acta Bot Boreali-Occident Sin, 2005, 25: 1107–1113 (in Chinese with English abstract)

[11]于卓, 秦永梅, 赵晓杰, 赵娜, 闫文芝. 优良饲用作物新品种——蒙农青饲1号高丹草选育. 中国草地, 2004, 26(2): 1–9

Yu Z, Qin Y M, Zhao X J, Zhao N, Yan W Z. New forage variety―Sorghum × Sudan grass of Mengnongqingsi 1# breeding. China Grassland, 2004, 26(2): 1–9 (in Chinese with English abstract)

[12]于卓, 赵晓杰, 赵娜, 秦永梅, 刘永伟. 蒙农青饲2号高丹草选育. 草地学报, 2004, 12: 176–182

Yu Z, Zhao X J, Zhao N, Qin Y M, Liu Y W. Sorghum × sudan grass of Mengnongqingsi 2# breeding. Acta Agres Sin, 2004, 12: 176–182 (in Chinese with English abstract)

[13]于卓, 马艳红, 李小雷, 刘志华, 周亚星, 李晓宇, 李长青. 蒙农3号高丹草选育. 中国草地学报, 2008, 30(6): 1–9

Yu Z, Ma Y H, Li X L, Liu Z H, Zhou Y X, Li X Y, Li C Q. Sorghum × Sudan grass of Mengnongqingsi 3# breeding. Chin J Grassland, 2008, 30(6): 1–9 (in Chinese with English abstract)

[14]詹秋文, 林平, 钱章强. 皖草3号的选育及其特征特性. 作物杂志, 2006, (4): 35–36

Zhan Q W, Lin P, Qian Z Q. The characteristic and breeding of Wancao 3#. Crops, 2006, (4): 35–36 (in Chinese)

[15]赵庆勇, 朱镇, 张亚东, 赵凌, 陈涛, 张巧凤, 王才林. SSR标记遗传距离与粳稻杂种优势的相关性分析. 中国水稻科学, 2009, 27: 141–147

Zhao Q Y, Zhu Z, Zhang Y D, Zhao L, Chen T, Zhang Q F, Wang C L. Correlation analysis between genetic distances based SSR and heterosis of japonica rice. Chin J Rice Sci, 2009, 27: 141–147 (in Chinese with English abstract)

[16]张培江, 才宏伟, 李焕朝, 杨联松, 张德泉, 白一松, 胡兴明, 许传万. RAPD分子标记水稻遗传距离及其与杂种优势的关系.安徽农业科学, 2000, 28: 697–700

Zhang P J, Cai H W, Li H C, Yang L S, Zhang D Q, Bai Y S, Hu X M, Xu C W. Relationship between genetic distance and heterosis of rice based on RAPD marker. J Anhui Agric Sci, 2000, 28: 697–700 (in Chinese with English abstract)

[17]张涛, 韩磊, 徐建第, 蒋开锋, 吴先军, 汪旭东, 郑家奎. 杂交香稻亲本遗传距离与产量杂种优势的相关性研究. 中国农业科学, 2006, 39: 831–835

Zhang T, Han L, Xu J D, Jiang K F, Wu X J, Wang X D, Zheng J K. Correlation analysis between genetic distance and yield heterosis of scented rice. Sci Agric Sin, 2006, 39: 831–835 (in Chinese with English abstract)

[18]袁力行, 傅骏骅, 刘新芝, 彭泽斌, 张世煌, 李新海, 李连城. 利用分子标记预测玉米杂种优势的研究. 中国农业科学, 2000, 33(6): 6–12

Yuan L X, Fu J H, Liu X Z, Peng Z B, Zhang S H, Li X H, Li L C. Prediction of heterosis of maize based on molecular marker. Sci Agric Sin, 2000, 33(6): 6–12 (in Chinese with English abstract)

[19]Zhang Q F, Gao Y J, Saghui Marrof M A, et al. Molecular divergence and hybrid performance in rice. Mol Breed, 1995, 1: 133–142

[20]张涛, 倪先林, 蒋开锋, 杨乾华, 万先齐, 曹应江. 水稻功能基因标记遗传距离与杂种优势的相关性研究. 中国水稻科学, 2009, 23: 567–572

Zhang T, Ni X L, Jiang K F, Yang Q H, Wan X Q, Cao Y J. Correlation between genetic distance based on molecular markers of functional genes and heterosis in rice. Chin J Rice Sci, 2009, 23: 567–572 (in Chinese with English abstract)

[21]逯晓萍, 云锦凤, 肖宇红, 米福贵, 李美娜, 尹利. 高丹草(高粱×苏丹草)产量及其构成因素的QTL定位与分析. 华北农学报, 2007, 22(4): 80–85

Lu X P, Yun J F, Xiao Y H, Mi F G, Li M N, Yin L. QTLs of yield related traits on Sorghum-Sudan grass. Acta Agric Boreali-Sin, 2007, 22(4): 80–85 (in Chinese with English abstract)

[22]逯晓萍, 云锦凤.高丹草遗传图谱构建及农艺性状基因定位研究. 草地学报, 2005, 13: 262–263

Lu X P, Yun J F. Research on genetic mapping and agronomic gene location of sorghum-sudan grass. Acta Agres Sin, 2005, 13: 262–263 (in Chinese with English abstract)

[23]Lu X P, Yun J F, Gao C P, Surya A. QTL Analysis of economically important traits in Sorghum bicolor × S. sudanense hybrid. Can J Plant Sci, 2011, 91: 81–90

[24]刘丹丹, 逯晓萍, 张瑞霞, 温莹, 薛春雷, 杨凯, 任瑞, 王亚男, 韩平安. 高丹草种质资源SRAP指纹图谱构建及遗传多样性分析.华北农学报, 2012, 27(2): 1–8

Liu D D, Lu X P, Zhang R X, Wen Y, Xue C L, Yang K, Ren R, Wang Y N, Han P A. SRAP fingerprint map and genetic diversity of Sorghum-Sudan germplasm. Acta Agric Boreali-Sin, 2012, 27(2): 1–8 (in Chinese with English abstract)

[25]温莹, 逯晓萍, 任锐, 米福贵, 韩平安, 薛春雷. 高丹草EST-SSR标记的开发及其遗传多样性. 遗传, 2013, 35: 225–232

Wen Y, Lu X P, Ren R, Mi F G, Han P A, Xue C L. Development of EST-SSR marker and genetic diversity analysis in Sorghum bicolor × S. sudanenes. Hereditas, 2013, 35: 225–232 (in Chinese with English abstract)

[26]逯晓萍, 云锦凤, 张雅慧, 薛春雷, 陈强.高丹草重组自交系群体的遗传变异与高产种质的创新. 华北农学报, 2009, 24(5): 90–95

Lu X P, Yun J F, Zhang Y H, Xue C L, Chen Q. Genetic variation of inbred population and creation of high yield germ on Sorghum bicolor × S. sudanense hybrid. Acta Agric Boreali-Sin, 2009, 24(5): 90–95 (in Chinese with English abstract)

[27]逯晓萍, 云锦凤, 米福贵, 陈强, 张雅慧, 薛春雷. 基于性状和分子标记的高丹草近等基因系的分离研究. 中国农业科学, 2010, 43(3): 468–473

Lu X P, Yun J F, Mi F G, Chen Q, Zhang Y H, Xue C L. Segregation of near isogene lines of Sorghum bicolor × S. sudanense based on traits and molecular marker. Sci Agric Sin, 2010, 43(3): 468–473 (in Chinese with English abstract)

[28]苏舒, 董维, 游录鹏, 黄守程, 戚金亮, 陆桂华, 黄应华, 杨永华. 高粱株高性状的QTL定位初步分析. 江苏农业科学, 2012, 40(3): 19–21

Su S, Dong W, You L P, Huang S C, Qi J L, Lu G H, Huang Y H, Yang Y H. Primary analysis of QTLs location related height of sorghum. Jiangsu Agric Sci, 2012, 40(3): 19–21 (in Chinese with English abstract)

[29]张南松, 陈军杰, 祝增荣.利用多重抽样估计稀有物种的种类数. 生物数学学报, 2002, 17: 455–460

Zhang N S, Chen J J, Zhu Z R. Multiple sampling evaluation of the number of rare species. J Math Bio, 2002, 17: 455–460 (in Chinese with English abstract)

[30]许国. Jackknife估计及其应用浅探. 山东师范大学学报, 2000, 15: 454–455

Xu G. Jackknife evaluation and application. J Shandong Norm Univ, 2000, 15: 454–455 (in Chinese with English abstract)

[31]Tams S H, Bauer E, Oettler G. Prospects for hybrid breeding in winter triticale: II. Relationship between parental genetic distance and specific combining ability. Plant Breed, 2006, 125: 331–336

[32]Hari D, Upadhyaya, Wang Y H. SSR markers linked to kernel weight and tiller number in sorghum identified by association mapping. Euphytica, 2012, 187: 401–410

[33]何光华, 侯磊, 李德谋, 罗小英, 牛国清, 唐梅, 裴炎.利用分子标记预测杂交水稻产量及其构成因素. 遗传学报, 2002, 29: 438–444

He G H, Hou L, Li D M, Luo X Y, Niu G Q, Tang M, Pei Y. Prediction of yield and related trait factors of hybrid rice with molecular marker. J Genet and Genom, 2002, 29: 438–444 (in Chinese with English abstract)

[34]游书梅, 曹应江, 郑家奎, 蒋开锋, 张涛, 杨莉, 杨乾华, 万先齐, 罗婧. 杂交籼稻子粒QTL标记遗传距离与粒重、粒重杂种优势的相关性. 植物遗传资源学报, 2012, 13: 1050–1054

You S M, Cao Y J, Zheng J K, Jiang K F, Zhang T, Yang L, Yang Q H, Wan X Q, Luo J. Correlation of genetic distance and seed weight heterosis based on QTL markers of hybrid rice. J Plant Genet Resour, 2012, 13: 1050–1054 (in Chinese with English abstract)

[35]武耀廷, 张天真, 朱协飞, 王广明. 陆地棉遗传距离与杂种F1、F2产量及杂种优势的相关分析.中国农业科学, 2002, 35: 22–28

Wu Y T, Zhang T Z, Zhu X F, Wang G M. Correlation of genetic distance, yield of F1 and F2 and hybrid heterosis. Sci Agric Sin, 2002, 35: 22–28 (in Chinese with English abstract)

[36]Vuylsteke M, Kuiper M, Stam P. Chromosomal regions involved in hybrid performance and heterosis: their AFLP(R)-based identification and practical use in prediction models. Heredity, 2000, 85: 208–218

Metrics

Viewed

Full text

327

HTML			PDF

Just accepted	Online first	Issue	Just accepted	Online first	Issue
0	0	0	0	0	327

From	Others	local

Times	26	301
Rate	8%	92%

Abstract

497

Just accepted	Online first	Issue

0	0	497

From	Others	local

Times	61	436
Rate	12%	88%

Cited

Web of Science	Crossref	ScienceDirect	Search for Citations in Google Scholar >>


This page requires you have already subscribed to WoS.

Shared

Discussed