欢迎访问作物学报，今天是 2025年5月1日 星期四
作物学报

作物学报 ›› 2014, Vol. 40 ›› Issue (03): 466-475.doi: 10.3724/SP.J.1006.2014.00466

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

高丹草遗传效应与杂种表现预测模型

逯晓萍1,刘丹丹1,王树彦1,米福贵2,韩平安1,吕二锁1   

  1. 1内蒙古农业大学农学院, 内蒙古呼和浩特010019；2内蒙古农业大学生态环境学院, 内蒙古呼和浩特010019
  • 收稿日期:2013-03-11 修回日期:2013-09-06 出版日期:2014-03-12 网络出版日期:2013-11-14
  • 基金资助:

    本研究由国家自然科学基金项目(31160302)资助。

Genetic Effects and Heterosis Prediction Model of Sorghum  bicolor × S.sudanense Grass

LU Xiao-Ping1,LIU Dan-Dan1,WANG Shu-Yan1,MI Fu-Gui2,HAN Ping-An1,LÜ Er-Suo1   

  1. 1 Agronomy College, Inner Mongolia Agricultural University, Huhhot 010019, China; 2 College of Ecology and Environmental Science, Inner Mongolia Agricultural University, Huhhot 010019, China
  • Received:2013-03-11 Revised:2013-09-06 Published:2014-03-12 Published online:2013-11-14

RichHTML

PDF

1036

被引次数

8

摘要：

以5个高粱不育系材料为母本, 18个优质苏丹草材料为父本, 按照遗传交配设计(NCII)配制成90个杂交组合。分别在内蒙呼市和包头两地, 利用与高丹草产量相关的QTL位点标记检测亲本间的遗传差异, 并将F1的8个性状表型值对亲本材料进行标记位点的筛选, 建立标记效应和标记型值估算体系。估算特异性位点对性状表现的效应及杂种标记型值, 进而分析杂种标记型值与杂种表现的相关性, 应用逐步回归分析法建立8个性状杂种表现的预测模型, 并通过Jackknife抽样技术检测模型的精确度和稳定性。结果显示, 在分别考虑显性、加性作用下8个性状的标记型值与表型值的相关系数平均为0.65, 各性状的可决系数较大(0.51~0.88), 而且两地结果趋势一致, 表明该预测模型稳定性强, 精确度较高。该模型对高丹草的杂种表现预测以及亲本选配都具有一定的指导意义。

关键词: 高丹草, 遗传效应, AFLP, SSR, SRAP, 杂种表现, 预测模型

Abstract:

Molecular markers-based heterosis prediction can provide some advices for Sorghum ×Sudan grass breeding. The 90 cross combinations were made according to the North Carolina design II (NCII) with five sorghum sterile lines as maternal parents and 18 Sudan grass lines as paternal parents. The field trials were carried out on the farms in Hohhot and Baotou to evaluate environmental effects. The parental genetic differences were compared by yield related QTL markers. The phenotypic values of eight traits for all F1 hybrids were investigated. The values were used for selecting significant marker loci on parental lines for setting up the evaluation system of marker effect and marker value. The specific loci were used for evaluating the trait effects and hybrid marker value, and for analyzing the correlation between the marker value and heterosis. The prediction models of the eight traits for the hybrid were constructed with the stepwise regression analysis. The Jackknife sampling method was used to test the accuracy and stability of the model. The result indicated that, considering dominance and additive effect separately, eight traits showed the average correlation coefficient of 0.65 between vigor value and phenotypic value. The coefficient of determination was from 0.51 to 0.88 in the eight traits. The results in two places were similar. The model could be instructive for heterosis prediction and parents selection.

Key words: Sorghum ×Sudan grassGenetic effect, AFLP, SSR, SRAP, Heterosis, Prediction model

[1]詹秋文, 钱章强. 高粱与苏丹草杂种优势利用的研究. 作物学报, 2004, 30: 73–77



Zhan Q W, Qian Z Q. Research of Sorghum-Sudan grass of heterosis utilization. Acta Agron Sin, 2004, 30: 73–77 (in Chinese with English abstract)



[2]王和平, 张福耀, 程庆军, 平俊爱, 杜志宏, 郭玉兰, 王晋中. 高粱-苏丹草杂交草的研究与利用. 杂粮作物, 2000, 20(4): 20–23



Wang H P, Zhang F Y, Cheng Q J, Ping J A, Du Z H, Guo Y L, Wang J Z. The Research and utilization of Sorghum-Sudan grass. Cereal Crops, 2000, 20(4): 20–23 (in Chinese)



[3]赵甘霖, 丁国祥, 熊洪, 曾庆曦. 我国高粱-苏丹草杂交种研究进展及应用前景. 西南农业学报, 2003, 16(4): 126–129



Zhao G L, Ding G X, Xiong H, Zeng Q X. Prospect of progress and application of Sorghum-Sudan grass in China. Southwest China J Agric Sci, 2003, 16(4): 126–129 (in Chinese with English abstract)



[4]Chung J M, Lee B C, Kim J S. Fine-scale structure among genetic individuals of clone-forming monotypic genus Echinosophora koreensis (Fabaceae). Ann Bot, 2006, 98: 165–173



[5]钱章强. 利用高粱雄性不育系选育饲用新品种. 中国草地, 1990, (6): 61–71



Qian Z Q. Breeding of new variety with male sterile line of sorghum. China Grassland, 1990, (6): 61–71 (in Chinese with English abstract)



[6]牟芝兰. 刈割周期对苏丹草叶片生长的影响. 草业科学, 1994, 11(6): 29–31



Mou Z L. Research of foliage growth of sorghum-sudan grass after cutting. Pratac Sci, 1994, 11(6): 29–31 (in Chinese with English abstract)



[7]陈才夫, 王槐三. 苏丹草×拟高粱种间杂种主要特性及细胞学分析. 南京农业大学学报, 1993, (2): 58–63



Chen C F, Wang H S. Main characteristics and cytological analysis of Sudan grass × sorghum interspecific hybrids. J Nanjing Agric Univ, 1993, (2): 58–63 (in Chinese with English abstract)



[8]孟钢. "先锋"高丹草-饲用高粱和苏丹草杂交品种. 世界农业, 2003, 6(6): 46–48



Meng G. “Pioneer” sorghum-Sudan grass ― hybrid of forage sorghum and Sudan grass. World Agric, 2003, 6(6): 46–48 (in Chinese)



[9]于卓, 赵晓杰, 秦永梅. 高粱与苏丹草2个杂交组合F2代的生育及细胞遗传学研究. 草业科学, 2004, 7: 13–17



Yu Z, Zhao X J, Qin Y M. Growth and cyto-genetics of F2 generation in Sorghum × Sudan grass combinations. Pratac Sci, 2004, 7: 13–17 (in Chinese with English abstract)



[10]赵晓杰, 于卓, 刘永伟, 逯晓萍. 高粱314A、13A与苏丹草杂种F1代的农艺特性及细胞学分析. 西北植物学报, 2005, 25: 1107–1113



Zhao X J, Yu Z, Liu Y W, Lu X P. Agronomic trait and cytology of hybrid F1 of sorghum (314A and 13A) × Sudan grass. Acta Bot Boreali-Occident Sin, 2005, 25: 1107–1113 (in Chinese with English abstract)



[11]于卓, 秦永梅, 赵晓杰, 赵娜, 闫文芝. 优良饲用作物新品种——蒙农青饲1号高丹草选育. 中国草地, 2004, 26(2): 1–9



Yu Z, Qin Y M, Zhao X J, Zhao N, Yan W Z. New forage variety―Sorghum × Sudan grass of Mengnongqingsi 1# breeding. China Grassland, 2004, 26(2): 1–9 (in Chinese with English abstract)



[12]于卓, 赵晓杰, 赵娜, 秦永梅, 刘永伟. 蒙农青饲2号高丹草选育. 草地学报, 2004, 12: 176–182



Yu Z, Zhao X J, Zhao N, Qin Y M, Liu Y W. Sorghum × sudan grass of Mengnongqingsi 2# breeding. Acta Agres Sin, 2004, 12: 176–182 (in Chinese with English abstract)



[13]于卓, 马艳红, 李小雷, 刘志华, 周亚星, 李晓宇, 李长青. 蒙农3号高丹草选育. 中国草地学报, 2008, 30(6): 1–9



Yu Z, Ma Y H, Li X L, Liu Z H, Zhou Y X, Li X Y, Li C Q. Sorghum × Sudan grass of Mengnongqingsi 3# breeding. Chin J Grassland, 2008, 30(6): 1–9 (in Chinese with English abstract)



[14]詹秋文, 林平, 钱章强. 皖草3号的选育及其特征特性. 作物杂志, 2006, (4): 35–36



Zhan Q W, Lin P, Qian Z Q. The characteristic and breeding of Wancao 3#. Crops, 2006, (4): 35–36 (in Chinese)



[15]赵庆勇, 朱镇, 张亚东, 赵凌, 陈涛, 张巧凤, 王才林. SSR标记遗传距离与粳稻杂种优势的相关性分析. 中国水稻科学, 2009, 27: 141–147



Zhao Q Y, Zhu Z, Zhang Y D, Zhao L, Chen T, Zhang Q F, Wang C L. Correlation analysis between genetic distances based SSR and heterosis of japonica rice. Chin J Rice Sci, 2009, 27: 141–147 (in Chinese with English abstract)



[16]张培江, 才宏伟, 李焕朝, 杨联松, 张德泉, 白一松, 胡兴明, 许传万. RAPD分子标记水稻遗传距离及其与杂种优势的关系.安徽农业科学, 2000, 28: 697–700



Zhang P J, Cai H W, Li H C, Yang L S, Zhang D Q, Bai Y S, Hu X M, Xu C W. Relationship between genetic distance and heterosis of rice based on RAPD marker. J Anhui Agric Sci, 2000, 28: 697–700 (in Chinese with English abstract)



[17]张涛, 韩磊, 徐建第, 蒋开锋, 吴先军, 汪旭东, 郑家奎. 杂交香稻亲本遗传距离与产量杂种优势的相关性研究. 中国农业科学, 2006, 39: 831–835



Zhang T, Han L, Xu J D, Jiang K F, Wu X J, Wang X D, Zheng J K. Correlation analysis between genetic distance and yield heterosis of scented rice. Sci Agric Sin, 2006, 39: 831–835 (in Chinese with English abstract)



[18]袁力行, 傅骏骅, 刘新芝, 彭泽斌, 张世煌, 李新海, 李连城. 利用分子标记预测玉米杂种优势的研究. 中国农业科学, 2000, 33(6): 6–12



Yuan L X, Fu J H, Liu X Z, Peng Z B, Zhang S H, Li X H, Li L C. Prediction of heterosis of maize based on molecular marker. Sci Agric Sin, 2000, 33(6): 6–12 (in Chinese with English abstract)



[19]Zhang Q F, Gao Y J, Saghui Marrof M A, et al. Molecular divergence and hybrid performance in rice. Mol Breed, 1995, 1: 133–142



[20]张涛, 倪先林, 蒋开锋, 杨乾华, 万先齐, 曹应江. 水稻功能基因标记遗传距离与杂种优势的相关性研究. 中国水稻科学, 2009, 23: 567–572



Zhang T, Ni X L, Jiang K F, Yang Q H, Wan X Q, Cao Y J. Correlation between genetic distance based on molecular markers of functional genes and heterosis in rice. Chin J Rice Sci, 2009, 23: 567–572 (in Chinese with English abstract)



[21]逯晓萍, 云锦凤, 肖宇红, 米福贵, 李美娜, 尹利. 高丹草(高粱×苏丹草)产量及其构成因素的QTL定位与分析. 华北农学报, 2007, 22(4): 80–85



Lu X P, Yun J F, Xiao Y H, Mi F G, Li M N, Yin L. QTLs of yield related traits on Sorghum-Sudan grass. Acta Agric Boreali-Sin, 2007, 22(4): 80–85 (in Chinese with English abstract)



[22]逯晓萍, 云锦凤.高丹草遗传图谱构建及农艺性状基因定位研究. 草地学报, 2005, 13: 262–263



Lu X P, Yun J F. Research on genetic mapping and agronomic gene location of sorghum-sudan grass. Acta Agres Sin, 2005, 13: 262–263 (in Chinese with English abstract)



[23]Lu X P, Yun J F, Gao C P, Surya A. QTL Analysis of economically important traits in Sorghum bicolor × S. sudanense hybrid. Can J Plant Sci, 2011, 91: 81–90



[24]刘丹丹, 逯晓萍, 张瑞霞, 温莹, 薛春雷, 杨凯, 任瑞, 王亚男, 韩平安. 高丹草种质资源SRAP指纹图谱构建及遗传多样性分析.华北农学报, 2012, 27(2): 1–8



Liu D D, Lu X P, Zhang R X, Wen Y, Xue C L, Yang K, Ren R, Wang Y N, Han P A. SRAP fingerprint map and genetic diversity of Sorghum-Sudan germplasm. Acta Agric Boreali-Sin, 2012, 27(2): 1–8 (in Chinese with English abstract)



[25]温莹, 逯晓萍, 任锐, 米福贵, 韩平安, 薛春雷. 高丹草EST-SSR标记的开发及其遗传多样性. 遗传, 2013, 35: 225–232



Wen Y, Lu X P, Ren R, Mi F G, Han P A, Xue C L. Development of EST-SSR marker and genetic diversity analysis in Sorghum bicolor × S. sudanenes. Hereditas, 2013, 35: 225–232 (in Chinese with English abstract)



[26]逯晓萍, 云锦凤, 张雅慧,  薛春雷, 陈强.高丹草重组自交系群体的遗传变异与高产种质的创新. 华北农学报, 2009, 24(5): 90–95



Lu X P, Yun J F, Zhang Y H, Xue C L, Chen Q. Genetic variation of inbred population and creation of high yield germ on Sorghum bicolor × S. sudanense hybrid. Acta Agric Boreali-Sin, 2009, 24(5): 90–95 (in Chinese with English abstract)



[27]逯晓萍, 云锦凤, 米福贵, 陈强, 张雅慧, 薛春雷. 基于性状和分子标记的高丹草近等基因系的分离研究. 中国农业科学, 2010, 43(3): 468–473



Lu X P, Yun J F, Mi F G, Chen Q, Zhang Y H, Xue C L. Segregation of near isogene lines of Sorghum bicolor × S. sudanense based on traits and molecular marker. Sci Agric Sin, 2010, 43(3): 468–473 (in Chinese with English abstract)



[28]苏舒, 董维, 游录鹏, 黄守程, 戚金亮, 陆桂华, 黄应华, 杨永华. 高粱株高性状的QTL定位初步分析. 江苏农业科学, 2012, 40(3): 19–21



Su S, Dong W, You L P, Huang S C, Qi J L, Lu G H, Huang Y H, Yang Y H. Primary analysis of QTLs location related height of sorghum. Jiangsu Agric Sci, 2012, 40(3): 19–21 (in Chinese with English abstract)



[29]张南松, 陈军杰, 祝增荣.利用多重抽样估计稀有物种的种类数. 生物数学学报, 2002, 17: 455–460



Zhang N S, Chen J J, Zhu Z R. Multiple sampling evaluation of the number of rare species. J Math Bio, 2002, 17: 455–460 (in Chinese with English abstract)



[30]许国. Jackknife估计及其应用浅探. 山东师范大学学报, 2000, 15: 454–455



Xu G. Jackknife evaluation and application. J Shandong Norm Univ, 2000, 15: 454–455 (in Chinese with English abstract)



[31]Tams S H, Bauer E, Oettler G. Prospects for hybrid breeding in winter triticale: II. Relationship between parental genetic distance and specific combining ability. Plant Breed, 2006, 125: 331–336



[32]Hari D, Upadhyaya, Wang Y H. SSR markers linked to kernel weight and tiller number in sorghum identified by association mapping. Euphytica, 2012, 187: 401–410



[33]何光华, 侯磊, 李德谋, 罗小英, 牛国清, 唐梅, 裴炎.利用分子标记预测杂交水稻产量及其构成因素. 遗传学报, 2002, 29: 438–444



He G H, Hou L, Li D M, Luo X Y, Niu G Q, Tang M, Pei Y. Prediction of yield and related trait factors of hybrid rice with molecular marker. J Genet and Genom, 2002, 29: 438–444 (in Chinese with English abstract)



[34]游书梅, 曹应江, 郑家奎, 蒋开锋, 张涛, 杨莉, 杨乾华, 万先齐, 罗婧. 杂交籼稻子粒QTL标记遗传距离与粒重、粒重杂种优势的相关性. 植物遗传资源学报, 2012, 13: 1050–1054



You S M, Cao Y J, Zheng J K, Jiang K F, Zhang T, Yang L, Yang Q H, Wan X Q, Luo J. Correlation of genetic distance and seed weight heterosis based on QTL markers of hybrid rice. J Plant Genet Resour, 2012, 13: 1050–1054 (in Chinese with English abstract)



[35]武耀廷, 张天真, 朱协飞, 王广明. 陆地棉遗传距离与杂种F1、F2产量及杂种优势的相关分析.中国农业科学, 2002, 35: 22–28



Wu Y T, Zhang T Z, Zhu X F, Wang G M. Correlation of genetic distance, yield of F1 and F2 and hybrid heterosis. Sci Agric Sin, 2002, 35: 22–28 (in Chinese with English abstract)



[36]Vuylsteke M, Kuiper M, Stam P. Chromosomal regions involved in hybrid performance and heterosis: their AFLP(R)-based identification and practical use in prediction models. Heredity, 2000, 85: 208–218
[1] 陈小红, 林元香, 王倩, 丁敏, 王海岗, 陈凌, 高志军, 王瑞云, 乔治军. 基于高基元SSR构建黍稷种质资源的分子身份证[J]. 作物学报, 2022, 48(4): 908-919.
[2] 张霞, 于卓, 金兴红, 于肖夏, 李景伟, 李佳奇. 马铃薯SSR引物的开发、特征分析及在彩色马铃薯材料中的扩增研究[J]. 作物学报, 2022, 48(4): 920-929.
[3] 王琰琰, 王俊, 刘国祥, 钟秋, 张华述, 骆铮珍, 陈志华, 戴培刚, 佟英, 李媛, 蒋勋, 张兴伟, 杨爱国. 基于SSR标记的雪茄烟种质资源指纹图谱库的构建及遗传多样性分析[J]. 作物学报, 2021, 47(7): 1259-1274.
[4] 韩贝, 王旭文, 李保奇, 余渝, 田琴, 杨细燕. 陆地棉种质资源抗旱性状的关联分析[J]. 作物学报, 2021, 47(3): 438-450.
[5] 刘少荣, 杨扬, 田红丽, 易红梅, 王璐, 康定明, 范亚明, 任洁, 江彬, 葛建镕, 成广雷, 王凤格. 基于农艺及品质性状与SSR标记的青贮玉米品种遗传多样性分析[J]. 作物学报, 2021, 47(12): 2362-2370.
[6] 郭艳春, 张力岚, 陈思远, 祁建民, 方平平, 陶爱芬, 张列梅, 张立武. 黄麻应用核心种质的DNA分子身份证构建[J]. 作物学报, 2021, 47(1): 80-93.
[7] 王恒波,祁舒婷,陈姝琦,郭晋隆,阙友雄. 甘蔗栽培种单倍体基因组SSR位点的发掘与应用[J]. 作物学报, 2020, 46(4): 631-642.
[8] 张红岩,杨涛,刘荣,晋芳,张力科,于海天,胡锦国,杨峰,王栋,何玉华,宗绪晓. 利用EST-SSR标记评价羽扇豆属(Lupinus L.)遗传多样性[J]. 作物学报, 2020, 46(3): 330-340.
[9] 解松峰,吉万全,张耀元,张俊杰,胡卫国,李俊,王长有,张宏,陈春环. 小麦重要产量性状的主基因+多基因混合遗传分析[J]. 作物学报, 2020, 46(3): 365-384.
[10] 张力岚, 张列梅, 牛焕颖, 徐益, 李玉, 祁建民, 陶爱芬, 方平平, 张立武. 黄麻SSR标记与纤维产量性状的相关性[J]. 作物学报, 2020, 46(12): 1905-1913.
[11] 刘荣, 王芳, 方俐, 杨涛, 张红岩, 黄宇宁, 王栋, 季一山, 徐东旭, 李冠, 郭瑞军, 宗绪晓. 利用2个F2群体整合中国豌豆高密度SSR遗传连锁图谱[J]. 作物学报, 2020, 46(10): 1496-1506.
[12] 叶卫军,陈圣男,杨勇,张丽亚,田东丰,张磊,周斌. 绿豆SSR标记的开发及遗传多样性分析[J]. 作物学报, 2019, 45(8): 1176-1188.
[13] 陈芳,乔麟轶,李锐,刘成,李欣,郭慧娟,张树伟,常利芳,李东方,阎晓涛,任永康,张晓军,畅志坚. 小麦新种质CH1357抗白粉病遗传分析及染色体定位[J]. 作物学报, 2019, 45(10): 1503-1510.
[14] 薛延桃,陆平,史梦莎,孙昊月,刘敏轩,王瑞云. 新疆、甘肃黍稷资源的遗传多样性与群体遗传结构研究[J]. 作物学报, 2019, 45(10): 1511-1521.
[15] 姚嘉瑜,张立武,赵捷,徐益,祁建民,张列梅. 黄麻全基因组SSR鉴定与特征分析[J]. 作物学报, 2019, 45(1): 10-17.
Viewed
Full text
325
HTML PDF
Just accepted Online first Issue Just accepted Online first Issue
0 0 0 0 0 325

  From Others local
  Times 26 299
  Rate 8% 92%

Abstract
489
Just accepted Online first Issue
0 0 489
  From Others local
  Times 61 428
  Rate 12% 88%

Cited
Web of Science  Crossref   ScienceDirect  Search for Citations in Google Scholar >>
 
This page requires you have already subscribed to WoS.
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2