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作物学报 ›› 2014, Vol. 40 ›› Issue (03): 487-496.doi: 10.3724/SP.J.1006.2014.00487

• 耕作栽培·生理生化 • 上一篇    下一篇

1,2,4-三氯苯胁迫对水稻分蘖盛期植株生长和生理特性的影响

丁秀文1,2,4,张国良2,3,*,戴其根1,*,朱青2   

  1. 1 扬州大学/江苏省作物遗传生理重点实验室 / 江苏省作物栽培生理重点实验室, 江苏扬州225009; 2淮阴工学院生命科学与化学工程学院 /江苏省生物质转化与过程集成工程实验室, 江苏淮安223003; 3中国科学院南京土壤研究所 / 土壤与农业可持续发展国家重点实验室, 江苏南京210008; 4江苏省丹阳市导墅镇农业服务中心, 江苏丹阳 212300
  • 收稿日期:2013-05-27 修回日期:2013-08-20 出版日期:2014-03-12 网络出版日期:2013-11-14
  • 通讯作者: 张国良, E-mail: hgzgl@sina.com, Tel: 0517-83559216; 戴其根, E-mail: qgdai2000@126.com, Tel: 0514-87979220
  • 基金资助:

    本研究由国家自然科学基金项目(31271639), 土壤与农业可持续发展国家重点实验室开放课题(Y052010016)和江苏省作物栽培生理重点实验室开放课题(K09008)资助。

Effects of 1,2,4-trichlorobenzene on Growth and Physiological Characteristics of Rice at Top Tillering Stage

DING Xiu-Wen1,2,4,ZHANG Guo-Liang2,3,*,DAI Qi-Gen1,*,ZHU Qing2   

  1. 1 Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Crop Cultivation and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China; 2 College of Life Science and Chemistry Engineering / Jiangsu Province Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huai’an 223003, China; 3 Institute of Soil Science / State Key Laboratory of Soil and Sustainable Agriculture, Chinese Academy of Sciences, Nanjing 210008, China; 4 Daoshu Agricultural Service Center of Danyang City, Jiangsu Province, Danyang 212300, China
  • Received:2013-05-27 Revised:2013-08-20 Published:2014-03-12 Published online:2013-11-14
  • Contact: 张国良, E-mail: hgzgl@sina.com, Tel: 0517-83559216; 戴其根, E-mail: qgdai2000@126.com, Tel: 0514-87979220

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摘要：

在土培条件下，以对1,2,4-三氯苯(TCB)敏感性显著差异的宁粳1号(敏感)和扬辐粳8号(耐性)为对象，研究了不同浓度1,2,4-三氯苯(TCB)胁迫对两种水稻分蘖盛期生长和生理特性的影响。结果表明，不同浓度TCB胁迫下，敏感基因型宁粳1号最长根长、株高、单穴分蘖数、地上部和地下部干物重随胁迫程度加深均显著递减; 耐性基因型扬辐粳8号在低浓度(20 mg kg-1) TCB胁迫下，最长根长、地上部和地下部干物重极显著增加，当中高浓度(40 mg kg-1、60 mg kg-1) TCB胁迫时，最长根长、株高、单穴分蘖数、地上部和地下部干物重显著下降。不同浓度TCB胁迫下，敏感基因型宁粳1号根系活力、叶绿素含量、叶片和根系可溶性蛋白质含量相对较低，叶片和根系超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性明显降低，O2?产生速率和丙二醛(MDA)含量显著增高; 耐性基因型扬辐粳8号在低浓度(20 mg kg-1) TCB胁迫下，根系活力、叶片和根系的可溶性蛋白含量、SOD、POD、CAT活性极显著增加，O2?产生速率和MDA含量极显著降低，当中等浓度(40 mg kg-1) TCB胁迫时，SOD、POD、CAT活性虽然增强，但O2?产生速率显著增加，MDA大量积累，当高浓度(60 mg kg-1) TCB胁迫时，其根系活力、叶绿素含量极显著降低，根系中SOD、CAT活性显著降低，O2?产生速率和MDA含量显著增加。总之，低浓度TCB胁迫下，长势良好、叶片和根系可溶性蛋白质含量高、抗氧化系统清除活性氧能力强、膜脂过氧化程度低是耐性基因型扬辐粳8号区别于敏感型宁粳1号的主要特征。

关键词: 水稻, 分蘖盛期, 1,2,4-三氯苯, 毒性, 生长发育, 抗氧化酶

Abstract:

A soil culture experiment was conducted to study the effects of 1,2,4-trichlorobenzene (TCB) on morphological and physiological indexes of rice at top tillering stage, using rice cultivars of Ningjing 1 (TCB sensitive genotype) and Yangfujing 8 (TCB tolerant genotype), with four treatments of TCB concentrations including 0 (CK), 20, 40 and 60 mg kg-1. The results indicated that the maximum root length, plant height, tillers per hill, shoot and root dry weight of Ningjing 1 were all decreased significantly (P<0.05) under different concentrations of TCB. Low TCB stress (20 mg kg-1) significantly increased Yangfujing 8’s maximum root length, shoot and root dry weight , root activity (P<0.01), while medium and high TCB stresses (40 and 60 mg kg-1) decreased maximum root length, plant height, tillers per hill, shoot and root dry weight significantly(P<0.05). Ningjing 1’s root activity, chlorophyll content, soluble protein content in leaves and roots were relatively low under different concentrations of TCB, at the same time, its activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in leaves and roots decreased, while O2? producing velocity and malnodialdehyde (MDA) content increased significantly (P<0.05). Low TCB stress (20 mg kg-1) significantly increased Yangfujing 8’s soluble protein content and activities of SOD, POD, CAT in leaves and roots, and significantly (P<0.05) decreased its O2? producing velocity and MDA content. Under medium TCB stress (40 mg kg-1), the activities of SOD, POD, CAT in leaves and roots were enhanced, however, its O2? producing velocity and MDA content increased significantly. High TCB stress (60 mg kg-1) decreased its root activity, chlorophyll content, the activities of SOD, POD, CAT in leaves and roots, and increased O2? producing velocity and MDA content significantly (P<0.05). In a word, compared with sensitive genotype, well growth vigor, high protein content in leaves and roots, better ability of antioxidant system in scavenging reactive oxygen speciesand lower membrane lipid peroxidation under low concentration TCB stress (20 mg kg-1) are regarded as the main features of TCB tolerant genotype in rice.

Key words: Rice, Top tillering stage, 1,2,4-trichlorobenzene, Toxicity, Growth and development, Antioxidant enzymes

[1]Miguel A S, Faure M, Ravanel P, Raveton M. Biological responses of maize (Zea mays) plants exposed to chlorobenzenes. Case study of monochloro-1,4-dichloro- and 1,2,4-trichloro-benzenes. Ecotoxicology, 2012, 21: 315–324



[2]周文敏, 傅德黔, 孙宗光. 中国水中优先控制污染物黑名单的确定.环境科学研究, 1991, 4(6): 9–12



Zhou W M, Fu D Q, Sun Z G. Determination of black list of China’s priority pollutants in water. Res Environ Sci, 1991, 4(6): 9–12(in Chinese with English abstract)



[3]Oliver B G, Niimi A J. Bioconcentration of chlorobenzenes from water by rainbow trout: Correlations with partition coefficients and environmental residues. Environ Sci Technol, 1983, 17: 287–291



[4]魏泰莉, 贾晓珊, 杜青平, 赖子尼, 庞世勋, 谢文平, 杨婉玲. 珠江口水、沉积物及水生动物中氯苯类有机物的含量及分布.环境科学学报, 2007, 27: 1717–1726



Wei T L, Jia X S, Du Q P , Lai Z N, Pang S X, Xie W P, Yang W L. Chlorobenzenes in the waters, sediments and aquatic animals from the Pearl River Estuary. Acta Scientiae Circumstantiae, 2007, 27: 1717–1726 (in Chinese with English abstract)



[5]杜青平, 贾晓珊, 袁保红. 1,2,4-三氯苯对水稻种子萌发及幼苗生长的毒性机理. 应用生态学报, 2006, 17: 2185–2188



Du Q P, Jia X S, Yuan B H. Toxic effects of 1,2,4-trichlorobenzene on rice seed germination and seedling growth. Chin J Appl Ecol, 2006, 17: 2185–2188 (in Chinese with English abstract)



[6]宋洋, 王芳, 蒋新. 微生物降解1,2,4-三氯苯研究进展. 土壤, 2011, 43: 343–349



Song Y, Wang F, Jiang X. Microbial degradation of 1,2,4-trichlorobenzene: a review. Soils, 2011, 43: 343–349 (in Chinese with English abstract)



[7]周霞, 余刚, 黄俊, 张祖麟, 胡洪营. 北京东南郊化工区土壤和植物中氯苯类有机物的残留及分布特征. 环境科学, 2007, 28: 249–254



Zhou X, Yu G , Huang J, Zhang Z L, Hu H Y. Residues and distribution characters of chlorobenzenes in soil and plants from Beijing Southeast Chemical Industry Zone. Environ Sci, 2007, 28: 249–254 (in Chinese with English abstract)



[8]崔健, 都基众, 杨泽, 马宏伟, 李霄. 沈阳市城郊土壤有机污染特征. 生态学杂志, 2011, 30: 2472–2477



Cui J, Du J Z, Yang Z, Ma H W, Li X. Characteristics of soil organic pollution in Shenyang suburbs of Liaoning Province. Chin J Ecol, 2011, 30: 2472–2477 (in Chinese with English abstract)



[9]蔡全英, 莫测辉, 吴启堂, 李桂荣. 微生物方法降低城市污泥的重金属含量研究进展.环境化学, 2002, 21(2): 139–143



Cai Q Y, Mo C H, Wu Q T, Li G R. Preliminary study on the content of chlorobenzenes in selected municipal sludge of China. Environ Chem, 2002, 21(2): 139–143 (in Chinese with English abstract)



[10]刘玉萍. 松花江水体氯苯类污染物的污染研究.环境科学与管理, 2006, 31(5): 91–92



Liu Y P. Study of Chlorobenzene’s pollution in the Songhua River. Environ Sci Managt, 2006, 31(5): 91–92 (in Chinese with English abstract)



[11]朱明吉, 郭志顺. 三峡库区重庆段有机污染研究. 三峡环境与生态, 2009, 2(2): 5–11



Zhu M J, Guo Z S. Research on organic pollution in Chongqing section of the Three Gorges Reservoir. Environ Ecol Three Gorges, 2009, 2(2): 5–11 (in Chinese with English abstract)



[12]石建省, 王昭, 张兆吉, 费宇红, 李亚松, 张凤娥, 陈京生, 钱永. 华北平原地下水有机污染特征初步分析. 生态环境学报, 2011, 20: 1695–1699



Shi J S, Wang Z, Zhang Z J, Fei Y H, Li Y S, Zhang F E, Chen J S, Qian Y. Preliminary analysis on the organic contamination of groundwater in the North China Plain. Ecol Environ Sci, 2011, 20: 1695–1699 (in Chinese with English abstract)



[13]Lee C L, Song H J, Fan M D. Concentrations of chlorobenzenes, hexachlorobutadiene and heavy metals in surficial sediments of Kaohsiung coast, Taiwan. Chemosphere, 2000, 41: 889–899



[14]Zhang J Y, Zhao W, Pan J, Qiu L M, Zhu Y M. Tissue-dependent distribution and accumulation of chlorobenzenes by vegetables in urban area. Environ Int, 2005, 31: 855–860



[15]Jaime D, Manuel R, Mario D. 1,2,4-trichlorobenzene flow characteristics in saturated homogeneous and stratified porous media. Water Air Soil Poll, 2006, 177: 3–17



[16]PACS-L: Discharge Standard of Water Pollutants. Beijing: Beijing Municipal Environmental Protection Bureau/Beijing Municipal Administration of Quality and Technology Supervision, 2005 (2013-05-26). http://www.bjepb.gov.cn/portal0/tab189/info5815.htm



[17]PACS-L: Standard for Drinking Water Quality. Beijing: Ministry of Public Health of the People’s Republic of China/Standardization Administration of the People’s Republic of China, 2007 (2013-05-26). http://www.moh.gov.cn/zwgkzt/pgw/201212/33644.shtml



[18]杨继富. 污水灌溉农业问题与对策. 水资源保护, 2000, (2): 4–8



Yang J F. The problem on agricultural sewage irrigation and countermeasures. Water Resour Prot, 2000, (2): 4–8 (in Chinese with English abstract)



[19]Macleod M, Mackay D. An assessment of the environmental fate and exposure of benzene and the ehlorobenzenes in Canada. Chemosphere, 1999, 38: 1777–1796 



[20]刘宛, 孙铁珩, 李培军, 周启星, 梁文举, 台培东, 许华夏, 张海荣.1,2,4-三氯苯胁迫对萌发大豆种子中活性氧的影响.应用生态学报, 2002, 13: 1655–1658



Liu W, Sun T H, Li P J, Zhou Q X, Liang W J, Tai P D, Xu H X, Zhang H R. Effects of 1,2,4-trichlorobenzene stress on active oxygen in germinated soybean seeds. Chin J Appl Ecol, 2002, 13: 1655–1658 (in Chinese with English abstract)



[21]刘宛, 孙铁珩, 李培军, 周启星, 台培东, 张春桂, 许华夏, 张海荣. 1,2,4-三氯苯胁迫对大豆下胚轴膜脂过氧化的影响. 农业环境保护, 2002, 21: 413–416



Liu W, Sun T H, Li P J, Zhou Q X, Tai P D, Zhang C G, Xu H X，Zhang H R. Effects of 1,2,4-trichlorobenzene stress on membrane lipid peroxidation in soybean hypocotyls. J Agro-Environ Prot, 2002, 21: 413–416 (in Chinese with English abstract)



[22]张国良, 陈文军, 王显, 金添, 戴其根, 孙国荣, 许轲, 霍中洋, 张洪程. 小麦苗期对1,2,4-三氯苯胁迫的生理响应.生态学报, 2008, 28: 4388–4395



Zhang G L, Chen W J, Wang X, Jin T, Dai Q G, Sun G R, Xu C, Huo Z Y, Zhang H C. Physiological reaction of wheat seedling to 1,2,4-trichlorobenzene stress. Acta Ecol Sin, 2008, 28: 4388–4395 (in Chinese with English abstract)



[23]丁艳, 葛才林, 王泽港, 杜庆才. 小麦幼苗对镉和1,2,4-三氯苯污染的响应.中国农业大学学报, 2011, 16: 48–52



Ding Y, Ge C L, Wang Z G, Du Q C. Response of Cd and 1,2,4-Trichlorobenzene pollutants on growth of wheat seedlings. J China Agric Univ, 2011, 16: 48–52 (in Chinese with English abstract)



[24]王泽港, 葛才林, 万定珍, 郦志文, 罗时石, 杨建昌.1,2,4-三氯苯和萘对水稻幼苗生长的影响. 农业环境科学学报, 2006, 25: 1402–1407



Wang Z G, Ge C L, Wan D Z, Li Z W, Luo S S, Yang J C. Effects of 1,2,4-trichlorobenzene and naphthalene on growth of rice seedling. J Agro-Environ Sci, 2006, 25: 1402–1407 (in Chinese with English abstract)



[25]张国良, 陈文军, 仇利民, 孙国荣, 戴其根, 张洪程. 不同基因型水稻苗期对1,2,4-三氯苯胁迫的生理响应. 作物学报, 2009, 35: 733–740



Zhang G L, Chen W J, Qiu L M, Sun G R, Dai Q G, Zhang H C. Physiological response to 1,2,4-trichlorobenzene stress of different rice genotypes in seedlings. Acta Agron Sin, 2009, 35: 733–740 (in Chinese with English abstract)



[26]王泽港, 骆剑峰, 高红明, 万定珍, 葛才林, 罗时石, 杨建昌. 1,2,4-三氯苯和萘对水稻抽穗期叶片光合特性的影响.中国农业科学, 2005, 38: 1113–1119



Wang Z G, LuoJ F, Gao H M, Wan D Z, Ge C L, Luo S S, Yang J C. Effects of 1,2,4-trichlorobenzene and naphthalene stress on photosynthetic characteristics of rice at heading period. Sci Agric Sin, 2005, 38: 1113–1119 (in Chinese with English abstract)



[27]陈文军, 张国良, 孙国荣, 戴其根, 张洪程, 陶金飞, 孙洁, 严林锋. 水稻耐1,2,4-三氯苯胁迫基因型的苗期筛选. 农业环境科学学报, 2008, 27: 1003–1008



Chen W J, Zhang G L, Sun G R, Dai Q G, Zhang H C, Tao J F, Sun J, Yan L F. Screening of tolerant rice genotypes to 1,2,4-trichlorobenzene stress at seedling stage. J Agro-Environ Sci, 2008, 27: 1003–1008 (in Chinese with English abstract)



[28]张志良. 植物生理学实验指导. 北京: 高等教育出版社, 2009. pp 30–31, 100, 125–126



Zhang Z L. The Guidance of Plant Physiological Experiment. Beijing: Higher Education Press, 2009. pp 30–31, 100, 125–126 (in Chinese)



[29]邹琦. 植物生理学实验指导.北京：中国农业出版社, 2000. pp 173–174



Zou Q. The Guidance of Plant Physiological Experiment. Beijing: China Agriculture Press, 2000. pp 173–174 (in Chinese)



[30]高俊凤. 植物生理学实验指导. 北京：高等教育出版社, 2006. pp 215–216



Gao J F. The Guidance of Plant Physiological Experiment. Beijing: Higher Education Press, 2006. pp 215–216 (in Chinese)



[31]郝建军. 植物生理学实验技术. 北京：化学工业出版社, 2007. pp 159–160



Hao J J. Experimental technique of Plant Physiological. Beijing: Chemical Industry Press, 2007. pp 159–160 (in Chinese)



[32]李忠光, 龚明. 植物中超氧阴离子自由基测定方法的改进. 云南植物研究, 2005, 27: 211–216



Li Z G, Gong M. Improvement of measurement method for superoxide anion radical in plant. Acta Bot Yunnanica, 2005, 27: 211–216 (in Chinese with English abstract)



[33]Wang M J, Jones K C. Behavior and fate of chlorobenzenes (CBs) introduced into soil-plant systems by sewage sludge application: a review. Chemosphere, 1994, 28: 1325–1360



[34]He Y W, Sun T H, Ou Z Q, Yediler A and Kettrup A. Fate of 1,2,4-trichlorobenzene (1,2,4-TCB) in soil-rice paddy system. Chemosphere, 1996, 32: 1381–1389



[35]于开源, 苏玉红, 刘涛, 梁永超. 三氯乙烯和三氯苯在水-玉米-空气系统中的传输过程研究. 农业环境科学学报, 2010, 29: 1702–1705



Yu K Y, Su Y H, Liu T, Liang Y C. Transport processes of trichloroethylene and trichlorobenzene across the water-corn-air interface. J Agro-Environ Sci, 2010, 29: 1702–1705 (in Chinese with English abstract)



[36]Ge C L, Wan D Z, Wang Z G, Ding Y, Wang Y L, Shang Q, Ma F, Luo S S. Response of rice roots to 1,2,4-trichlorobenzene stress. Acta Agron Sin, 2007, 33: 1991–2000



[37]金阳. 1,2,4-三氯苯对水稻伤害机理及水稻耐性机制的研究. 扬州大学硕士学位论文, 2003. pp 20–25



Jin Y. Study on the Mechanisms of l,2,4-Triehlorobenzene Damaging Rice. MS. Dissertation of Yangzhou University, 2003 (in Chinese with English abstract)



[38]张波. 小麦对氧乐果和1,2,4-三氯苯响应的生物学机理. 石河子大学硕士学位论文, 2010. pp 18–22



Zhang B. Physiological Responses of Wheat Seedlings to Omethoate and 1,2,4-trichlorobenzene Stress. MS Theses of Shihezi University, 2010 (in Chinese with English abstract)



[39]Ge C L, Wan D Z, Wang Z G, Ding Y, Wang Y L, Shang Q, Ma F, Luo S S. A proteomic analysis of rice seedings responding to 1,2,4-trichlorobenzene stress. J Environ Sci, 2008, 10: 309–319



[40]许振华, 谢传晓. 植物microRNA与逆境响应研究进展. 遗传, 2010, 32: 1018–1030



Xu Z H, Xie C X. Advances in plant microRNA and stresses response. Hereditas (Beijing), 2010, 32: 1018–1030 (in Chinese with English abstract)



[41]何耀武, 孙铁琦, 区自清. 1,2,4一三氯苯在土壤中的降解. 应用生态学报, 1996, 7: 429–434



He Y W, Sun T H, Ou Z Q. Degradation of 1,2,4-trichlorobenzene (1,2,4-TCB) in soil. Chin J Appl Ecol, 1996, 7: 429–434 (in Chinese with English abstract)



[42]李勇, 黄占斌, 王文萍, 黄震, 颜丙磊, 曹杨, 王诗宇. 重金属铅镉对玉米生长及土壤微生物的影响. 农业环境科学学报, 2009, 28: 2241–2245



Li Y, Huang Z B, Wang W P, Huang Z, Yan B L, Cao Y, Wang S Y. Effects of heavy metals lead and cadmium on Zea mays L. growth and the soil microorganism. J Agro-Environ Sci, 2009, 28: 2241–2245 (in Chinese with English abstract)
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