欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2014, Vol. 40 ›› Issue (02): 369-379.doi: 10.3724/SP.J.1006.2014.00369

• 研究简报 • 上一篇    

新疆自育陆地棉品种SSR遗传多样性分析

艾先涛1,2,梁亚军2,沙红2,王俊铎2,郑巨云2,吐尔逊江2,多力坤2,李雪源2,*,华金平3,*   

  1. 1新疆农业大学, 乌鲁木齐 830052；2新疆农业科学院经济作物研究所, 乌鲁木齐 830091；3中国农业大学植物遗传育种系 / 杂种优势研究与利用教育部重点实验室 / 作物遗传改良北京市重点实验室, 北京 100193
  • 收稿日期:2013-01-21 修回日期:2013-08-16 出版日期:2014-02-12 网络出版日期:2013-10-22
  • 通讯作者: 华金平, E-mail: jinping_hua@cau.edu.cn; 李雪源, E-mail: xjmh2338@163.com
  • 基金资助:

    本研究由新疆自然科学基金项目(2012211B47)和国家高技术研究发展计划(863计划)项目(2012AA101108)资助。

Genetic Diversity Analysis on Local Upland Cotton Cultivars in Xinjiang Based on SSR Markers

AI Xian-Tao1, 2,LIANG Ya-Jun2,SHA Hong2,WANG Jun-Duo2,ZHENG Ju-Yun2,Tu-Er-Xun-Jiang2,DUO Li-Kun2,LI Xue-Yuan2, *,HUA Jin-Ping3,*   

  1. 1Xinjiang Agricultural University , Urumqi 830052, China; 2 Economic Crop Research Institute, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China; 3 Department of Plant Genetics and Breeding / Key Laboratory of Crop Heterosis and Utilization of Ministry of Education / Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
  • Received:2013-01-21 Revised:2013-08-16 Published:2014-02-12 Published online:2013-10-22
  • Contact: 华金平, E-mail: jinping_hua@cau.edu.cn; 李雪源, E-mail: xjmh2338@163.com

RichHTML

PDF

1023

被引次数

32

摘要：

利用SSR标记对94份新疆自育陆地棉品种的基因组进行分析，研究新疆陆地棉品种的遗传多样性。结果: 从分布于棉花全基因组的206对SSR标记中筛选出54对具有稳定多态性的引物, 共检测出153个多态性位点, 每对引物的等位变异为2~6个，平均为2.93个；基因型多样性(H′)变幅为0.0439–0.7149，平均为0.4491；引物多态信息含量(PIC)为0.0430–0.6640，平均为0.3831。表明SSR标记在品种间可以反映较丰富的遗传多样性信息。94份品种间成对遗传相似系数变幅为0.3846~0.9835, 71.9%的品种相似系数在0.601~0.800内，反映出新疆陆地棉品种间的遗传相似性相对较高。根据UPGMA 聚类分析，在阈值为0.63时，将94份品种划分为2个类群，说明新疆陆地棉品种间遗传关系相对简单，品种的遗传基础相对狭窄，品种遗传组分差异较小，总体上遗传多样性不够丰富；分子聚类结果与品种本身遗传系谱背景和演变趋势吻合度较高，符合品种的真实特性。研究证明，自育品种在分子水平上差异不大，需要努力拓宽品种选育的遗传基础。

关键词: 陆地棉, 遗传背景, SSR, 遗传多样性, 亲缘关系

Abstract:

Xinjiang, the largest prior cotton production region in China, has made remarkable genetic improvement in upland cotton (Gossypium hirsutum L.). There has been a substantial raise in yield potential, cotton-planting area and total production in Xinjiang since 1970s. The new upland cotton cultivars have contributed a lot to yield increase, in which Xinjiang-bred upland cotton varieties play an important role in cotton production in both Northern and Southern Xinjiang. However, seldom research on genetic basis of genetic diversity and pedigree relationship has been evaluated in upland cotton varieties in Xinjiang so far. This study aimed to reveal the genetic diversity of 94 upland cotton varieties in Xinjiang by genome analysis using SSR markers. The results indicated that SSR molecular markers could be employed in revealing the genetic diversity. One hundred and fifty-three polymorphic lociwere detected by amplified with 54 pairs of primers with allelic variation of 2-6 per primer pair and the average of 2.93. The genotypic diversity (H’) spanned from 0.0439 to 0.7149, with the average of 0.4491. The PIC value ranged from 0.0430 to 0.6640, and the average was 0.3831. The coefficients of genetic similarity among 94 tested cultivars varied from 0.3846 to 0.9835. And more than 71.9% of similarity coefficients fell into the interval of 0.601–0.800, indicating that the genetic similarity among the cultivars was high, whereas the genetic diversity might not be abundant. According to UPGMA cluster analysis, 94 tested cultivars were divided into two clusters when the threshold valve was given as 0.63, showing the relatively simple genetic relationship among the upland cotton varieties in Xinjiang, and relatively low genetic diversity and narrow genetic basis. The results of molecular clustering on genetic diversity of upland cotton coincided in large degree with the pedigree relationship. In conclusion, there is no much difference on molecular level among Xinjiang-bred upland cotton varieties. Materials with much wider genetic backgrounds are suggested to be used to diversify the genetic basis of cotton varieties in Xinjiang.

Key words: Upland cotton, Genetic background, SSR, Genetic diversity, Genetic relationship

[1]周盛汉. 中国棉花品种系谱图. 成都: 四川科学技术出版社, 2000



Zhou S H. China Cotton Varieties Pedigree Diagram. Sichuan Publishing House of Science & Technology, 2000 (in Chinese)



[2]董玉琛, 刘旭. 中国作物野生近缘植物及其保护. 见: 中国科学院生物多样性委员会等编. 生物多样性与人类未来. 北京: 中国林业出版社, 1998, pp 24–29



Dong Y C, Liu X. Wild relative crops and their protection in China. In: Biodiversity Committee, CAS, etc. eds. Biological Diversity and the Future of Human Beings, Beijing: Chinese Forestry Press 1998, pp 24–29 (in Chinese)



[3]朱四元, 陈金湘, 刘爱玉, 李瑞莲, 严跃文, 唐海明. 利用SSR标记对不同类型抗虫棉品种的遗传多样性分析. 湖南农业大学学报, 2006, 32: 469–472



Zhu S Y, Chen J X, Liu A Y, Li R L, Yan Y W, Tang H M. Genetic diversity analysis and identification of insect-resistant cottons based upon SSR markers. J Hunan Agric Univ (Nat Sci), 2006, 32: 469–472 (in Chinese with English abstract)



[4]武耀廷, 张天真, 殷剑美. 利用分子标记和形态学性状检测的陆地棉栽培品种遗传多样性. 遗传学报, 2001, 28: 1040–1050



Wu Y T, Zhang T Z, Yin J M. Genetic diversity detected by DNA markers and phenotypes in upland cotton. Acta Genet Sin, 2001, 28: 1040–1050 (in Chinese with English abstract)



[5]李武, 倪薇, 林忠旭, 张献龙. 海岛棉遗传多样性的SRAP标记分析. 作物学报, 2008, 34: 893–898



Li W, Ni W, Lin Z X, Zhang X L. Genetic diversity analysis of sea-island cotton cultivars using SRAP markers. Acta Agron Sin, 2008, 34(5): 893–898 (in Chinese with English abstract)



[6]郭旺珍,周兆华,张天真. RAPD鉴定棉花抗(耐)黄萎病品种(系)的遗传变异研究. 江苏农业学报,1999, 15(1): 1–6



Guo W Z, Zhou Z H, Zhang T Z. A study on cotton genetic variation of Verticillium wilt resistance based on RAPDs. J Jiangsu Agric, 1999, 15(1): 1–6 (in Chinese with English abstract)



[7]凌磊, 李廷春, 李正鹏. 利用SRAP标记分析彩色棉与白色棉的遗传差异. 中国农学通报, 2009, 25(16): 32–38



Ling L, Li T C, Li Z P. Genetic difference analysis between colored cotton and white cotton by SRAP markers. Chin Agric Sci Bull, 2009, 25(16): 32–38 (in Chinese with English abstract)



[8]卫泽, 孙学振, 宋宪亮. 国内外57份棉花种质资源的遗传多样性研究. 山东农业科学, 2010, 6: 13–18



Wei Z, Sun X Z, Song X L. Genetic diversity of 57 cotton germplasm resources from China and overseas. Shandong Agric Sci, 2010, (6): 13–18 (in Chinese with English abstract)



[9]徐秋华, 张献龙, 聂以春. 长江、黄河两棉区陆地棉品种遗传多样性比较研究. 遗传学报, 2001, 8: 683–690



Xu Q H, Zhang X L, Nie Y C. Genetic diversity evaluation of cultivars (L.) from the Chang Jiang River Valley and the Huanghe Valley by RAPD. Acta Genet Sin, 2001, 28: 683–690 (in Chinese with English abstract)



[10]刘文欣, 孔繁玲, 郭志丽, 张群远, 彭慧茹, 付小琼, 杨付新. 建国以来我国棉花品种遗传基础的分子标记分析. 遗传学报, 2003, 30: 560–570



Liu W X, Kong F L, Guo Z L, Zhang Q Y, Peng H R, Fu X Q, Yang F X. Analysis about genetic basis of cotton cultivars in China since 1949 with molecular markers. Acta Genet Sin, 2003, 30: 560–570 (in Chinese with English abstract)



[11]宋国立, 崔荣霞, 王坤波, 郭立平, 黎绍惠, 王春英, 张香娣. 改良CTAB法快速提取棉花DNA. 棉花学报, 1998, 10(5): 273–275



Song G L, Cui R X, Wang K B, Guo L P, Li S H, Wang C Y, Zhang X D. The improved CTAB law rapid extraction of genomic DNA from cotton. Acta Gossypii Sin, 1998, 10: 273–275 (in Chinese with English abstract)



[12]Xiao J, Wu K, Fang D D, Stelly D M, Yu J, Cantrell R G. New SSR markers for Use in cotton (Gossypium spp.) improvement. J Cotton Sci, 2009, 13: 75–157



[13]艾先涛, 李雪源, 秦文斌, 孙国清, 吐尔逊江, 莫明. 新疆陆地棉育种遗传组分拓展研究. 分子植物育种, 2005, 3: 575–578



Ai X T, Li X Y, Qin W B, Sun G Q, Tu E X J, Mo M. The extend study on genetic composition of upland cotton breeding in Xinjiang. Mol Plant Breed, 2005, 3: 575–578 (in Chinese with English abstract)



[14]贺道华, 邢宏宜, 李婷婷, 汤益, 曾舟. 92份棉花资源遗传多样性的SSR分析. 西北植物学报, 2010, 30: 1557–1564



He D H, Xing H Y, Li T T, Tang Y, Zeng Z. Genetic diversity of 92 cotton accessions evaluated with SSR markers. Northwest Agric Univ, 2010, 30: 1557–1564 (in Chinese with English abstract)



[15]高伟, 刘方, 黎绍惠, 王春英, 张香娣, 王玉红, 王坤波. 利用SSR分析四倍体棉种多态性.作物学报, 2010, 36: 1902–1909



Gao W, Liu F, Li S H, Wang C Y, Zhang X D, Wang Y H, Wang K B. Genetic diversity of allotetraploid cotton based on SSR markers. Acta Agron Sin, 2010, 36: 1902−1909 (in Chinese with English abstract)



[16]别墅, 孔繁玲, 周有耀, 张光梅, 张群远, 王孝纲. 中国三大主产棉区棉花品种遗传多样性的RAPD及其与农艺性状关系的研究. 中国农业科学, 2001, 34: 597–603



Bie S, Kong F L, Zhou Y Y, Zhang G M, Zhang Q Y, Wang X G. Genetic diversity analysis representative elite cotton varieties in three main cotton regions in China by RAPD and its relation with agronomic characteristics. Sci Agric Sin, 2001, 34: 597–603 (in Chinese with English abstract)



[17]陈光. 我国陆地棉基础种质及其衍生品种的遗传多样性. 中国农业科学院研究生院硕士学位论文, 2005



Chen G. Genetic Diversity of Basic Germplasm and Their Offsprings of Upland Cotton in China. MS Thesis of Graduate School of Chinese Academy of Agricultural Sciences, 2005 (in Chinese with English abstract)



[18]杜雄明, 刘国强. 论我国棉花育种的基础种质. 植物遗传资源学报. 2004, 5: 69–74



Du X M, Liu G Q. Basic Germplasm for Cotton Breeding in China. J Plant Genet Resour, 2004, 5: 69–74 (in Chinese with English abstract)



[19]薛艳, 张新宇, 沙红, 李雪源, 孙杰, 李保成. 新疆早熟棉品种SSR指纹图谱构建与品种鉴别. 棉花学报, 2010, 22: 360–366



Xue Y, Zhang X Y, Sha H, Li X Y, Sun J, Li B C. Construction of Fingerprinting Map Based on SSR and Identification of Cultivars for Earliness Cultivars in Upland Cotton in Xinjiang. Acta Gossypii Sin, 2010, 22: 360–366 (in Chinese with English abstract)



[20]黄滋康, 季道藩. 中国棉花遗传育种学. 济南: 山东科学技术出版社, 2003



Hang Z K, Ji D F. China Cotton Genetics and Breeding. Jinan: Shandong Science & Technology Press, 2003



[21]孙杰, 褚贵新, 张文辉, 金文虹. 新疆特早熟棉区棉花品种主要性状演变趋势研究. 中国棉花, 1999, 26(7): 14–16



Sun J, Zhe G X, Zhang W H, Jin W H. The main character trend of especially early maturing varieties of cotton in Xinjiang. China’s Cotton, 1999, 26(7): 14–16 (in Chinese with English abstract)



[22]黄滋康. 中国棉花品种及其系谱. 北京: 中国农业出版社, 2007



Hang Z K. China's cotton varieties and its pedigree. Beijing: China Agricultural Press, 2007



[23]Yu F F, Huaxia Y F, Lu W J, Wu C G, Cao X C, Guo X Q. GhWRKY15, a member of the WRKY transcription factor family identified from cotton (Gossypium hirsutum L.), is involved in disease resistance and plant development, BMC Plant Biol, 2012, 12: 144



[24]Zhang Y, Wang X F, Li Z K, Zhang G Y. Assessing genetic diversity of cotton cultivars using genomic and newly developed expressed sequence tag-derived microsatellite markers. Genet Mol Res, 2011, 10: 1462–1470
[1] 肖颖妮, 于永涛, 谢利华, 祁喜涛, 李春艳, 文天祥, 李高科, 胡建广. 基于SNP标记揭示中国鲜食玉米品种的遗传多样性[J]. 作物学报, 2022, 48(6): 1301-1311.
[2] 陈小红, 林元香, 王倩, 丁敏, 王海岗, 陈凌, 高志军, 王瑞云, 乔治军. 基于高基元SSR构建黍稷种质资源的分子身份证[J]. 作物学报, 2022, 48(4): 908-919.
[3] 张霞, 于卓, 金兴红, 于肖夏, 李景伟, 李佳奇. 马铃薯SSR引物的开发、特征分析及在彩色马铃薯材料中的扩增研究[J]. 作物学报, 2022, 48(4): 920-929.
[4] 王琰琰, 王俊, 刘国祥, 钟秋, 张华述, 骆铮珍, 陈志华, 戴培刚, 佟英, 李媛, 蒋勋, 张兴伟, 杨爱国. 基于SSR标记的雪茄烟种质资源指纹图谱库的构建及遗传多样性分析[J]. 作物学报, 2021, 47(7): 1259-1274.
[5] 马燕斌, 王霞, 李换丽, 王平, 张建诚, 文晋, 王新胜, 宋梅芳, 吴霞, 杨建平. 玉米光敏色素A1基因(ZmPHYA1)在棉花中的转化及分子鉴定[J]. 作物学报, 2021, 47(6): 1197-1202.
[6] 韩贝, 王旭文, 李保奇, 余渝, 田琴, 杨细燕. 陆地棉种质资源抗旱性状的关联分析[J]. 作物学报, 2021, 47(3): 438-450.
[7] 刘少荣, 杨扬, 田红丽, 易红梅, 王璐, 康定明, 范亚明, 任洁, 江彬, 葛建镕, 成广雷, 王凤格. 基于农艺及品质性状与SSR标记的青贮玉米品种遗传多样性分析[J]. 作物学报, 2021, 47(12): 2362-2370.
[8] 郭艳春, 张力岚, 陈思远, 祁建民, 方平平, 陶爱芬, 张列梅, 张立武. 黄麻应用核心种质的DNA分子身份证构建[J]. 作物学报, 2021, 47(1): 80-93.
[9] 孙倩, 邹枚伶, 张辰笈, 江思容, Eder Jorge de Oliveira, 张圣奎, 夏志强, 王文泉, 李有志. 基于SNP和InDel标记的巴西木薯遗传多样性与群体遗传结构分析[J]. 作物学报, 2021, 47(1): 42-49.
[10] 赵孟良,王丽慧,任延靖,孙雪梅,侯志强,杨世鹏,李莉,钟启文. 257份菊芋种质资源表型性状的遗传多样性[J]. 作物学报, 2020, 46(5): 712-724.
[11] 王恒波,祁舒婷,陈姝琦,郭晋隆,阙友雄. 甘蔗栽培种单倍体基因组SSR位点的发掘与应用[J]. 作物学报, 2020, 46(4): 631-642.
[12] 张红岩,杨涛,刘荣,晋芳,张力科,于海天,胡锦国,杨峰,王栋,何玉华,宗绪晓. 利用EST-SSR标记评价羽扇豆属(Lupinus L.)遗传多样性[J]. 作物学报, 2020, 46(3): 330-340.
[13] 张力岚, 张列梅, 牛焕颖, 徐益, 李玉, 祁建民, 陶爱芬, 方平平, 张立武. 黄麻SSR标记与纤维产量性状的相关性[J]. 作物学报, 2020, 46(12): 1905-1913.
[14] 刘荣, 王芳, 方俐, 杨涛, 张红岩, 黄宇宁, 王栋, 季一山, 徐东旭, 李冠, 郭瑞军, 宗绪晓. 利用2个F2群体整合中国豌豆高密度SSR遗传连锁图谱[J]. 作物学报, 2020, 46(10): 1496-1506.
[15] 刘易科,朱展望,陈泠,邹娟,佟汉文,朱光,何伟杰,张宇庆,高春保. 基于SNP标记揭示我国小麦品种(系)的遗传多样性[J]. 作物学报, 2020, 46(02): 307-314.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2