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作物学报 ›› 2014, Vol. 40 ›› Issue (12): 2104-2117.doi: 10.3724/SP.J.1006.2014.02104

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

新陆早棉花品种DNA指纹图谱的构建及遗传多样性分析

聂新辉1,2,**,尤春源2,**,李晓方3,秦江鸿2,黄聪1,郭欢乐1,王夏青1,赵文霞1,林忠旭1,*   

  1. 1 华中农业大学作物遗传改良国家重点实验室, 湖北武汉 430070; 2石河子农业科学研究院棉花研究所, 新疆石河子 832000；3长江大学，湖北荆州 434025
  • 收稿日期:2014-04-02 修回日期:2014-09-16 出版日期:2014-12-12 网络出版日期:2014-10-16
  • 通讯作者: 林忠旭, E-mail: linzhongxu@mail.hzau.edu.cn ** 同等贡献 (Contributed equally to this work)
  • 基金资助:

    本研究由公益性行业(农业)科研专项(201303008),“十二五”兵团重点领域科技攻关项目(2011BA001)和新疆兵团石河子科技攻关项目(2009NY05)。

Construction of DNA Fingerprinting and Analysis of Genetic Diversity for Xinluzao Cotton Varieties

NIE Xin-Hui1,2,**,YOU Chun-Yuan2,**,LI Xiao-Fang3,QIN Jiang-Hong2,HUANG Cong1,GUO Huan-Le1,WANG Xia-Qing1,ZHAO Wen-Xia1LIN Zhong-Xu1,*   

  1. 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; 2 Cotton Institute, Shihezi Academy of Agricultural Sciences, Xinjiang 832000, China?; 3Changjiang University, Jingzhou 434025, China
  • Received:2014-04-02 Revised:2014-09-16 Published:2014-12-12 Published online:2014-10-16
  • Contact: 林忠旭, E-mail: linzhongxu@mail.hzau.edu.cn ** 同等贡献 (Contributed equally to this work)

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摘要：

以新疆2013年前审定的51个新陆早常规棉花品种为材料, 从5000对SSR引物中筛选出多态性高、稳定性好、且定位在棉花26条染色体上(每条染色体上选择2~3对)的75对核心引物，检测到多态性位点226个, 每个标记检测到的基因型位点数在2~12之间, 平均为3.01个；引物多态信息量(PIC)值介于0.0799~0.8752之间, 平均值为0.6624。结果显示, 在51份新陆早棉花常规品种中, 可利用特征引物将21份品种一次性区分开。利用40对引物可以完全区分开新陆早51份常规品种, 并构建供试品种的指纹图谱。同时利用NTSYS-pcV2.10软件聚类分析表明, 51个新陆早棉花品种遗传相似系数变化范围为0.4269~0.9873, 平均值为0.7071, 说明新陆早棉花品种之间遗传多样性较狭窄；遗传相似系数矩阵和聚类分析将51个新陆早品种分为4大类型, 与原品种选育系谱高度吻合。

关键词: 新陆早常规品种, SSR, DNA 指纹图谱, 遗传多样性

Abstract:

Fifty-one Xinluzao conventional cotton varieties authorized before 2013 were detected with 75 pairs of primers with high polymorphism, good repeatability, and even distribution on 26 chromosomes (2–3 pairs from each chromosome) selected from 5000 pairs of SSR primers. A total of 226 polymorphic loci were obtained, and each marker detected 2–12 polymorphic loci with an average of 3.01; the polymorphism information content ranged from 0.0799 to 0.8752, with an average of 0.6624. The results showed that 21 varieties could be differentiated by characteristic primers, the rest 30 varieties could be identified by primer conbinations. In the end, 40 characteristic primers and combinated primers could completely differentiate the 51 Xinluzao cotton varieties from each other. DNA fingerprinting of the 51 Xinluzao conventional varieties were constructed with the 40 pairs of SSR markers. NTSYS-pcV2.10 software was used to analyze genetic diversity of the 51 conventional varieties, the results indicated that genetic similarity coefficient for the varieties ranged from 0.4269 to 0.9873, with an average of 0.7071, showing the narrow genetic diversity in Xinluzao cotton varieties. The 51 Xinluzao cotton varieties were divided into four types with the genetic similarity coefficient matrix and cluster analysis, which were strongly consistent with their pedigrees.

Key words: Xinluzao conventional cotton varieties, SSR, DNA fingerprinting, Genetic diversity

[1]张玉翠. 我国棉花品种SSR指纹数据库的初步构建. 中国农业科学院研究生院硕士论文, 2012. pp 1–8



Zhang Y C. Construction a Primary SSR Fingerprint Database for Cotton Varieties. MS Thesis of Graduate School of Chinese Academy of Agricultural Sciences, Beijing, China, 2012. pp 1–8 (in Chinese with English abstract)



[2]张玉翠, 杨伟华, 匡猛, 许红霞, 王延琴, 周大云, 冯新爱, 苏畅. 32个棉花主栽品种DNA指纹图谱构建及遗传多样性分析. 棉花学报, 2012, 24: 120–126



Zhang Y C, Yang W H, Kuang M, Xu H X, Wang Y Q, Zhou D Y, Feng X A, Su C. Construction of DNA fingerprinting and analysis of genetic diversity with SSR markers for 32 leading cotton varieties. Cotton Sci, 2012, 24: 120–126 (in Chinese with English abstract)



[3]Zhang H Y, Wang H, Guo S, Yi R, Guo Y G, Yi Q W, Yong X. Identification and validation of a core set of microsatellite markers for genetic diversity analysis in watermelon, Citrullus lanatus Thunb. Matsum. & Nakai. Euphytica, 2012, 186: 324–342



[4]郭旺珍, 张天真, 潘家驹, 何金龙. 我国棉花主栽品种的RAPD指纹图谱研究. 农业生物技术学报, 1996, 4: 129–134



Guo W Z, Zhang T Z, Pan J J, He J L. Analysis of RAPD fingerprinting on main cotton varieties in China. J Agric Biotechnol, 1996, 4: 129–134 (in Chinese with English abstract)



[5]Multani D S, Lyon B R. Genetic fingerprinting of Australian cotton varietys with RAPD markers. Genome, 1995, 38: 1005–1008



[6]Iqbal M J, Aziz N, Saeed N A, Zafar Y, Malik K A. Genetic diversity evaluation of some elite cotton varieties by RAPD analysis. Theor Appl Genet, 1997, 94: 139–144



[7]武耀廷, 张天真, 郭旺珍, 殷剑美. 陆地棉品种SSR标记的多态性及用于杂交种纯度检测的研究. 棉花学报, 2001, 13: 131–133



Wu Y T , Zhang T Z, Guo W Z, Yin J M. Detecting polymorphism among upland cotton (Gossypium hirsutum L.) cultivars and their roles in seed purity of hybrids with SSR markers. Cotton Sci, 2001, 13: 131–133 (in Chinese with English abstract)



[8]马轩, 杜雄明, 孙君灵. 18个彩色棉品系的SSR指纹分析. 植物遗传资源学报, 2003, 4: 305–310



Ma X, Du X M, Sun J L. SSR fingerprinting analysis on 18 colored cotton lines. J Plant Genet Resourc, 2003, 4: 305–310 (in Chinese with English abstract)



[9]姜伟, 李雪源, 何觉民, 陆建农. 新疆8个棉花品种(系)的指纹图谱分析. 华北农学报, 2007, 22: 89–92



Jiang W, Li X Y, He J M, Lu J N. Fingerprinting analysis for 8cultivars (Lines) of Xinjiang. Acta Agric Boreali-Sin, 2007, 22: 89–92 (in Chinese with English abstract)



[10]李武, 倪薇, 林忠旭, 张献龙等. 海岛棉遗传多样性的SRAP标记分析. 作物学报, 2008, 34: 893−898



Li W, Ni W, Lin Z X, Zhang X L. Genetic diversity analysis of sea-island cotton cultivars using SRAP markers. Acta Agron Sin, 2008, 34: 893−898 (in Chinese with English abstract)



[11]潘兆娥, 王希文, 孙君灵, 周忠丽, 贾银华, 何守朴, 王杰, 王立如, 庞保印, 杜雄明. 中棉所48的SSR数字指纹图谱的构建. 中国农学通报, 2010, 26(7): 31–35



Pan Z E, Wang X W, Sun J L, Zhou Z L, Jia Y H, He S P, Wang J, Wang L R, Pang B Y, Du X M. Construction of digital fingerprint of Zhongmiansuo 48. Chine Agric Sci Bull, 2010, 26(7): 31–35 (in Chinese with English abstract)



[12]薛艳, 李保成, 张新宇, 沙红, 孙杰. 新疆早熟棉品种SSR分子标记体系的建立. 石河子大学学报(自然科学版) , 2011, 29(1): 1–5



Xue Y, Li B C, ZHang X Y, Sha H, Sun J. Optimization of amplification reaction system and construction of DNA fingerprinting of local upland cotton variety in Xinjiang. J Shihezi Univ (Nat Sci), 2011, 29(1): 1–5



[13]李成奇, 王晓芸, 张晓飞, 王清连.百棉系列棉花自交系品种(系)SSR指纹图谱构建. 棉花学报, 2011, 23: 228–234



Li Ch Q, Wang X Y, Zhang X F, Wang Q L. SSR fingerprinting establishment of baimian series cotton inbred varieties (lines). Cotton Sci, 2011, 23: 228–234



[14]孙宁, 杨付新, 付小琼, 王秀玲, 刘逢举, 李亚丽, 汤磊鹏. 国家棉花区试新品种的SSR指纹图谱分析. 棉花学报, 2011, 23: 279–283



Sun N, Yang F X, Fu X Q, Wang X L, Liu F J, Li Y L, Tang L P. SSR marker fingerprinting of cotton varieties in national regional trails. Cotton Sci, 2011, 23: 279–283 (in Chinese with English abstract)



[15]匡猛, 杨伟华, 许红霞, 王延琴, 周大云, 冯新爱. 中国棉花主栽品种DNA指纹图谱构建及SSR标记遗传多样性分析. 中国农业科学, 2011, 44: 20–27



Kuang M, Yang W H, Xu H X, Wang Y Q, Zhou D Y, Feng X A. Construction of DNA Fingerprinting and analysis of genetic diversity with SSR markers for cotton major cultivars in China. Sci Agric Sin, 2011, 44: 20–27 (in Chinese with English abstract)



[16]赵亮, 蔡彩平, 梅鸿献, 郭旺珍. 用于区别不同棉花品种基因组特征的微卫星位点筛选. 作物学报, 2012, 38: 1810–1817



Zhao L, Cai C P, Mei H X, Guo W Z. Screening of microsatellite loci for identifying genome barcoding of cotton cultivars. Acta Agron Sin, 2011, 44: 20–27 (in Chinese with English abstract)



[17]程保山, 万志兵, 洪德林. 35个粳稻品种SSR指纹图谱的构建及遗传相似性分析. 南京农业大学学报, 2007, 30(3): 1–8



Cheng B S, Wan Z B, Hong D L. Establishment of SSR fingerprint map and analysis of genetic similarity among 35 varieties in japonica rice. J Nanjing Agric Univ, 2007, 30(3): 1–8 (in Chinese with English abstract)



[18]李根英, Dreisigacker S, Warburton M L, 夏先春, 何中虎, 孙其信. 小麦指纹图谱数据库的建立及SSR分子标记试剂盒的研发. 作物学报, 2006, 32: 1771–1778



Li G Y, Susanne Dreisigacker, Marilyn L Warburton, Xia X C, He Z H, Sun Q X. Development of a fingerprinting database and assembling an SSR reference kit for genetic diversity analysis of wheat. Acta Agron Sin, 2006, 32: 1771–1778 (in Chinese with English abstract)



[19]赵久然, 王凤格, 郭景伦, 陈刚, 廖琴, 孙世贤, 陈如明, 刘龙洲.中国玉米新品种DNA指纹库建立系列研究. 玉米科学, 2003, 11(2): 3–5



Zhao J R, Wang F G, Guo J L, Chen G, Miao Q, Sun S X, Chen R M, Liu L Z. Series of research on establishing DNA fingerprinting pool of Chinese new maize varieties. J Maize Sci, 2003, 11(2): 3–5, 8 (in Chinese with English abstract)



[20]徐军, 刘艳华, 任民, 牟建民, 张兴伟, 陈雅琼, 王志德. 普通烟草种质资源的SSR标记与指纹图谱分析. 中国烟草科学, 2011, 32(2): 62–65



Xu J, Liu Y H, Ren M, Mou J M, Zhang X W, Chen Y Q, Wang Z D. SSR fingerprint map analysis of tobacco germplasms. Chin Tob Sci, 2011, 32(2): 62–65 (in Chinese with English abstract)



[21]田清震, 盖钧镒, 喻德跃, 吕慧能, 贾继增. 我国野生大豆与栽培大豆AFLP指纹图谱研究. 中国农业科学, 2001, 34: 465–468



Tian Q Z, Gai J Y, Yu D Y, Lü H N, Jia J Z. AFLP fingerprint analysis of G. soja and G. max in China. Sci Agric Sin, 2001, 34: 465–468 (in Chinese with English abstract)



[22]白静. 杂交棉品种SSR核心引物的筛选与真实性和纯度鉴定. 华中农业大学硕士论文, 湖北武汉, 2011, pp 45–50



Bai J. Screening of SSR Core Primer and the Identification of Genuineness and Purity on Hybrid Cotton. MS Thesis of Huazhong Agricultural University, Wuhan, China, 2011. pp 45–50 (in Chinese with English abstract)



[23]匡猛, 杨伟华, 许红霞, 王延琴, 周大云, 冯新爱, 王俊芳. 分子标记技术在棉花品种鉴定上的研究进展. 棉花学报, 2009, 21: 330–334



Kuang M, Yang W H, Xu H X, Wang Y Q, Zhou D Y, Feng X A, Wang J F. Research progress of molecular marker technology applied in cotton variety identification. Cotton Sci, 2009, 21: 330–334 (in Chinese with English abstract)



[24]Union for the Protection of New Varieties of Plants. Guidelines for Molecular Marker Selection and Database Construction//BMT Guidelines (Proj.3) . Geneva: UPOV, 2005. p 326



[25]陆徐忠, 马琳, 倪金龙, 张小娟, 杨剑波. 基于DNA 指纹建立农作物品种身份证的方法探析. 中国农学通报, 2014, 30(9): 248–252



Lu X Z, Ma L, Ni J L, Zhang X J, Yang J B. Perspectives on the Establishment of A Crop Identity System Based on DNA Fingerprinting. Chin Agric Sci Bull, 2014, 30(9): 248–252 (in Chinese with English abstract)



[26]Coryell V H, Jessen H, Schupp J M, Webb D, Keim P. Allele-specific hybridzation markers for soybean. Theor Appl Genet, 1999, 98: 690–696



[27]曲鲁江, 李显耀, 杜志强, 张龙超, 杨宁. 微卫星PCR产物变性与非变性银染检测PAGE方法的比较. 遗传, 2004, 26: 522–524



Qu L J, Li X Y, Du Z Q, Zhang L C, Yang N. The comparision of denatured non-denatured PAGE-silver-sataining detection of PCR product of microsatellites. Hereditas(Beijing), 2004, 26(4): 522–524 (in Chinese with English abstract)



[28]艾先涛, 梁亚军, 沙红, 王俊铎, 郑巨云, 吐尔逊江, 多力坤, 李雪源, 华金平. 新疆自育陆地棉品种SSR遗传多样性分析. 作物学报, 2014, 40: 369−379



Ai X T, Liang Y J, Sha H, Wang J D, Zheng J Y, Tu E X J, Duo L K, Li X Y, Hua J P. Genetic diversity analysis on local upland cotton cultivars in Xinjiang based. Acta Agron Sin, 2014, 40: 369−379 (in Chinese with English abstract)



[29]Liu D Q, Guo X P, Lin Z X, Nie Y C, Zhang X L. Genetic diversity of Asian cotton ( L.) in China evaluated by microsatellite analysis. Genet Resourc Crop Evol, 2006, 53: 1145–1152



[30]Ni X Y, Lei W X, Zhao J Y. Establishment of molecular identity ID Brassica napus varieties using SSR markers. Cruciferous oil crops and sustainable development. In: The Paper Abstract of 12th International Conference on Rapeseed. Monmouth Junction: Scientific American Publishing Company, 2007. p 166



[31]高运来, 朱荣胜, 刘春燕, 李文福, 蒋洪蔚, 李灿东, 姚丙晨, 胡国华, 陈庆山. 黑龙江部分大豆品种分子ID的构建. 作物学报, 2009, 35: 211–218



Gao Y L, Hu R S, Liu C Y, Li W F, Jiang H W, Li C D, Yao B C, Hu G H, Chen Q S. Establishment of molecular ID in soybean vari eties in Heilongjiang, China. Acta Agron Sin, 2009, 35: 211–218 (in Chinese with English abstract)



[32]宋海斌, 崔喜波, 马鸿艳, 朱子成, 栾非时. 基于SSR标记的甜瓜品种(系)DNA 指纹图谱库的构建. 中国农业科学, 2012, 45: 2676–2689



Song H B, Cui X B, Ma H Y, Zhu Z C, Luan F S. Construction of DNA fingerprint database based on SSR marker for varieties (lines) of Cucumis melo L. Sci Agric Sin, 2012, 45(13): 2676–2689 (in Chinese with English abstract)



[33]陆徐忠, 倪金龙, 李莉, 汪秀峰, 马卉, 张小娟, 杨剑波. 利用SSR分子指纹和商品信息构建水稻品种身份证. 作物学报, 2014, 40: 1–9



Lu X Z, NI J L, Li L, Wang X F, Ma H, Zhang X J, Yang J B. Construction of rice variety based on ID used SSR fingerprint and commodity information. Acta Agron Sin, 2014, 40: 1–9 (in Chinese with English abstract)
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