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作物学报 ›› 2015, Vol. 41 ›› Issue (09): 1372-1383.doi: 10.3724/SP.J.1006.2015.01372

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆异黄酮及其组分含量的遗传分析与QTL检测

梁慧珍1, 余永亮1, 杨红旗1, 许兰杰1, 董薇1, 牛永光1, 张海洋1, 刘学义2, 方宣钧3   

  1. 1河南省农业科学院芝麻研究中心, 河南郑州 450002; 2山西省农业科学院经济作物研究所, 山西汾阳 032200; 3 海南省热带农业资源开发利用研究所, 海南三亚 572025
  • 收稿日期:2015-03-03 出版日期:2015-09-12 网络出版日期:2015-09-12
  • 作者简介:第一作者联系方式: E-mail:lhzh66666@163.com, Tel: 0371-65751589
  • 基金资助:
    本研究由河南省科技创新杰出人才计划(114200510002), 国家转基因生物新品种培育科技重大专项(2014ZX0800402B), 河南省重点科技攻关计划项目(132102110091)和河南省农业科学院专项资金(201315603, 201315615, 201218334)资助

Genetic Analysis and QTL Mapping of Isoflavone Contents and Its Components in Soybean

LIANG Hui-Zhen1, YU Yong-Liang1, YANG Hong-Qi1, XU Lan-Jie1, DONG Wei1, NIU Yong-Guang1, ZHANG Hai-Yang1, LIU Xue-Yi2, FANG Xuan-Jun3   

  1. 1 Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 2 Industrial Crop Institute, Shanxi Academy of Agricultural Sciences, Fenyang 032200, China; 3 Hainan Provincial Institute of Tropical Agriculture Resources, Sanya 572025, China
  • Received:2015-03-03 Published:2015-09-12 Published online:2015-09-12

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摘要： 以栽培大豆晋豆23为母本, 以山西农家品种大豆灰布支黑豆为父本杂交衍生的447个RIL作为供试群体构建遗传图谱, 利用高效液相色谱法定性、定量测定样品中的异黄酮及其组分含量。采用主基因+多基因混合遗传分离分析法和WinQTLCart 2.5复合区间作图法, 对大豆异黄酮及其组分含量进行混合遗传分析和QTL定位。结果表明, 大豆苷、黄豆苷元、染料木素、染料木苷、大豆苷元和异黄酮总含量分别受4、4、2、3、2和2对主基因控制, 并有多基因修饰。检测到44个与大豆异黄酮及其组分含量相关的QTL, 与大豆苷、染料木素、黄豆苷元、大豆苷元、染料木苷和异黄酮总含量相关的QTL分别有10、9、4、7、8和6个。连续2年分别检测到与大豆苷、染料木苷、黄豆苷元和异黄酮关联, 分别位于标记区间satt430~satt359、satt038~satt570、satt197~sat_128和satt249~satt285的稳定表达QTL, 可尝试用于分子标记辅助育种。

关键词: 大豆, 异黄酮含量, 遗传分析, QTL

Abstract: A set of 447 recombinant inbred lines (RILs) derived from the cross between cultivars Jingdou 23 (female parent) and Huibuzhi (semi-wild, male parent) was used to construct a new map. Isoflavone content and its components were quantitatively and qualitatively evaluated by using high performance liquid chromatography (HPLC). We analyzed inheritance and detected QTLs for isoflavone content and its components in soybean seeds using major gene plus polygene mixed inheritance analysis and WinQTLCart 2.5 composite interval mapping. The results showed that daidzin, daidzein, genistein, genistin, glycitin, and total isoflavone contents were controlled by four, four, two, three, two and two main-genes, respectively. However, polygene effects were not detected in the study. Forty-four quantitative trait loci (QTLs) for isoflavone contents and its components were mapped, including ten for daidzin, nine for genistein, four for daidzein, seven for glycitin, eight for genistin, and six for total isoflavone content. The stable QTLs related to daidzin, genistin, glycitin, isoflavone content were respectively detected to be located in the intervals of satt430-satt359, satt038-satt570, satt197-sat_128, and satt249-satt285 during two years, which could be used in marker-assisted selection (MAS) for soybean breeding.

Key words: Soybean, Isoflavone content, Genetic analysis, Quantitative trait loci

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