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作物学报 ›› 2015, Vol. 41 ›› Issue (11): 1657-1662.doi: 10.3724/SP.J.1006.2015.01657

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

重组型大豆花叶病毒河北分离物序列特征及侵染性

林静1,2,杨永庆2,3,侯文焕2,杨春燕2,谢令琴1,*,智海剑3,张孟臣2,*   

  1. 1 河北农业大学农学院，河北保定 071001; 2 河北省农林科学院粮油作物研究所 / 国家大豆改良中心石家庄分中心 / 农业部黄淮海大豆生物学与遗传育种重点实验室 / 河北省作物遗传育种实验室，河北石家庄 050035; 3 南京农业大学作物遗传与种质创新国家重点实验室 / 农业部大豆生物学与遗传育种重点实验室 / 国家大豆改良中心，江苏南京 210095
  • 收稿日期:2015-04-04 修回日期:2015-07-10 出版日期:2015-11-12 网络出版日期:2015-08-12
  • 基金资助:

    本研究由国家自然科学基金项目(31401409，31471522)，国家现代农业产业技术体系建设专项(CARS-004-PS06)，国家转基因生物新品种培育重大专项(2014ZX0800402B，2014ZX08004001)和河北省科技支撑计划项目(14226309D)资助。

Sequence and Pathogenicity of Recombined Soybean Mosaic Virus Isolate from Hebei Province, China

LIN Jing1,2,YANG Yong-Qing2,3,HOU Wen-Huan2,YANG Chun-Yan2,XIE Ling-Qin1,*,ZHI Hai-Jian3,ZHANG Meng-Chen2,*   

  1. 1 College of Agronomy, Agricultural University of Hebei, Baoding, 071001; 2 Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences / National Soybean Improvement Center Shijiazhuang Sub-Center / Huang-Huai-Hai Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture / Hebei Laboratory of Crop Genetics and Breeding, Shijiazhuang 050035, China; 3.National Key Laboratory for Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture, Nanjing 210095, China
  • Received:2015-04-04 Revised:2015-07-10 Published:2015-11-12 Published online:2015-08-12

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摘要：

重组型大豆花叶病毒(soybean mosaic virus, SMV-R)是一种新SMV类型，在我国多个大豆产区广泛流行。本研究对一个重组型SMV河北分离物(HB-RS)进行全基因组测序,比较与非重组型SMV在侵染4个大豆品种后病毒浓度积累的差异。结果显示，除poly-A尾巴外，HB-RS (NCBI登录号为KR065437)由9993个核苷酸组成，包含一个开放阅读框(open reading frame, ORF)，翻译后形成3202个氨基酸，系统进化分析结果显示HB-RS分离物与另外两个重组型SMV分离物聚在一组。抗性鉴定结果显示，4个品种对HB-RS和Sc6平均病情指数分别为59.5和60.5，相同大豆品种对不同的株系(分离物)可能呈现不同的症状和抗性表现，其中冀豆17对Sc6和HB-RS分别表现高抗和中抗，表明大豆对SMV的抗性存在一定的株系(分离物)专化性。此外，HB-RS在4个品种中的浓度积累均高于Sc6，在南农1138-2病毒浓度最高，达522 U，其次为五星1号(471 U)和冀黄13 (199 U)，最低为冀豆17，仅90 U。说明HB-RS在寄主体内更具有生存适应性，不同品种对SMV存在抗性差异。冀豆17可作为抗性品种和亲本进一步推广。

关键词: 重组型大豆花叶病毒, 基因组, 病毒浓度, 侵染

Abstract:

Recombined Soybean mosaic virus (SMV-R), which is prevalent in many soybean production regions, is a novel type of SMV. To clarify the characteristics of recombinant SMV structure and pathogenicity of SMV-R, we sequenced the whole genomes of a SMV-R isolate (HB-RS) from Hebei province of China, and compered the difference of SMV infection and accumulation in four soybean cultivars with SMV-R. The results showed that besides the poly-A tail, HB-RS consists of 9993 nucleotides, encoding only one open reading frame and 3202 amino acids. Phylogenetic analysis showed that the HB-RS isolate was clustered with other two recombined SMV isolates. Resistance identification results showed that the average disease index of four cultivars resistant to HB-RS and Sc6 was 59.5 and 60.5, respectively. The same soybean cultivar had different symptoms and resistance levels to different strains (isolates), Jidou 17 showed high resistance to Sc6 and moderate resistance to HB-RS. These results indicated that soybean resistance to SMV exists strain (isolate) specialization. Additionally, the pathogenicity test showed that accumulation of HB-RS in four cultivars was higher than that of Sc6, indicating that HB-RS is more adaptable to the host plants. The host with highest accumulation of HB-RS was Nannong 1138-2, with 522-fold of reference virus accumulation, and the following was Wuxing 1 (471 U), Jihuang 13 (199 U) and, Jidou 17 (only 90 U), suggesting that HB-RS has a more survival adaptability in the soybean host. However different resistance levels to HB-RS were observed in various soybean cultivars and Jidou 17 could be used as a resistant cultivar in production or parents in further breeding.

Key words: Recombined SMV, Genome, Virus titer, Pathogenicity

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