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作物学报 ›› 2016, Vol. 42 ›› Issue (05): 641-647.doi: 10.3724/SP.J.1006.2016.00641

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用简化基因组技术分析甘薯种间单核苷酸多态性

石璇1,王茹媛1,唐君2,李宗芸1,*,罗永海1,*   

  1. 1江苏师范大学生命科学学院,江苏徐州221116;2中国农科院甘薯研究所 / 国家甘薯改良中心,江苏徐州 221121
  • 收稿日期:2015-10-19 修回日期:2016-01-11 出版日期:2016-05-12 网络出版日期:2016-02-18
  • 通讯作者: 罗永海, E-mail: yhluo@jsnu.edu.cn; 李宗芸, E-mail: zongyunli@jsnu.edu.cn
  • 基金资助:

    本项目获得江苏高校优势学科建设工程资助项目, 农业部农作物保护项目(2015NWB006), 江苏省自然科学基金面上项目(BK2012579, BK20141146)和江苏省高校自然科学研究重大项目(12KJA180001)资助。

Analysis of Interspecific SNPs in Sweetpotato Using a Reduced-Representation Genotyping Technology

SHI Xuan1,WANG Ru-Yuan1,TANG Jun2,LI Zong-Yun1,*,LUO Yong-Hai1,*   

  1. 1 School of Life Science, Jiangsu Normal University, Xuzhou 221116, China; 2 Sweetpotato Research Institute, Chinese Academy of Agricultural Sciences / National Sweetpotato Improvement Centre, Xuzhou 221121, China
  • Received:2015-10-19 Revised:2016-01-11 Published:2016-05-12 Published online:2016-02-18
  • Contact: Luo Yonghai, E-mail: yhluo@jsnu.edu.cn; 李宗芸, E-mail: zongyunli@jsnu.edu.cn
  • Supported by:

    This study was supported by the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, the Project on Crop Conservation Funded by the Ministry of Agriculture of China (2015NWB006), the General Projects Funded by Natural Science Foundation of Jiangsu Province (BK2012579, BK20141146), and the Major Project Funded by Natural Science Foundation of Jiangsu Higher Education Institutions (12KJA180001).

摘要:

选用Xushu18 (6x)和Nancy Hall (6x)、2个二倍体I. trifida (2x)品系(4597-10和4597-21)、四倍体I. trifida (4x)、六倍体I. trifida (6x)、二倍体I. temussima (2x)和I. littorallis (2x)以简化基因组测序技术SLAF-seq测序,获得724 589个SLAF标签,其中多态性SLAF标签35 310个。通过序列分析,获得40 765个有效单核苷酸多态(SNP),并用这些SNP分析了8个种质的群体结构和系统发生树。结果表明,利用简化基因组测序技术SLAF-seq能高效、低成本地开发出大量可用于群体遗传分析的SNP标记;通过构建进化树发现甘薯栽培种和野生种I. trifida的亲缘关系比较近。这些分析结果为进一步研究甘薯栽培种的起源提供了基础数据。

关键词: 甘薯, SLAF-seq, 分子标记, SNP, 进化分析

Abstract:

Xushu18 (2x), Nancy Hall (6x), I. trifida (2x) 4597-10, I. trifida (2x) 4597-21, I. trifida (4x), I. trifida (6x), I. temussima (2x), and I. littorallis (2x) were used as experimental materials for sequencing by specific-locus amplified fragment sequencing (SLAF-seq), a high-throughput reduced-representation genotyping technology. In total, 724 589 SLAF tags were obtained and 40 765 SNPs were identified out of 35 310 polymorphic SLAF tags. A total of 40 765 single nucleotide polymorphisms (SNPs) were obtained by sequence analysis. Population structure and phylogenetic relationship of eight germplasm were analyzed using the SNP dataset, which suggests that SLAF-seq can be used to develop large-scale SNPs for population genetic analysis, effectively and economically. Our analysis revealed that the relationship between sweet potato cultivars and the wild species I. trifida is closer. These results provide empirical data for further study of the origin of sweet potato.

Key words: Sweetpotato, SLAF-seq, Molecular marker, SNP, Phylogenetic analysis

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