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作物学报 ›› 2016, Vol. 42 ›› Issue (10): 1448-1461.doi: 10.3724/SP.J.1006.2016.01448

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

河南省审定花生品种的指纹图谱构建

孙子淇1,2,张新友2,*,徐静2,张忠信2,刘华2,严玫2,董文召2,黄冰艳2,韩锁义2,汤丰收2,刘志勇1,*   

  1. 1 中国农业大学农学院，北京 100193；2 河南省农业科学院经济作物研究所 / 农业部黄淮海油料作物重点实验室 / 河南省油料作物遗传改良重点实验室，河南郑州 450002
  • 收稿日期:2016-01-29 修回日期:2016-06-20 出版日期:2016-10-12 网络出版日期:2016-06-27
  • 通讯作者: 刘志勇, E-mail: zyliu@genetics.ac.cn, Tel: 010-64806422; 张新友, E-mail: haasz@126.com, Tel: 0371-65729560
  • 基金资助:

    本研究由河南省重大科技专项(141100110600), 国家现代农业产业技术体系建设专项(CARS-14)，河南省现代农业产业技术体系建设项目(S2012-5)和河南省农业科学院自主创新专项基金资助。

DNA Fingerprinting of Peanut (Arachis hypogaea L.) Varieties Released in Henan Province

SUN Zi-Qi1,2,ZHANG Xin-You2,*,XU Jing2,ZHANG Zhong-Xin2,LIU Hua2,YAN Mei2,DONG Wen-Zhao2,HUANG Bing-Yan2,HAN Suo-Yi2,TANG Feng-Shou2,LIU Zhi-Yong1,*   

  1. 1 College of Agronomy, China Agricultural University, Beijing 100193, China; 2 Industrial Crops Research Institute, Henan Academy of Agricultural Sciences / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry of Agriculture / Henan Provincial Key Laboratory for Genetic Improvement of Oil Crops, Zhengzhou 450002, China
  • Received:2016-01-29 Revised:2016-06-20 Published:2016-10-12 Published online:2016-06-27
  • Contact: 刘志勇, E-mail: zyliu@genetics.ac.cn, Tel: 010-64806422; 张新友, E-mail: haasz@126.com, Tel: 0371-65729560
  • Supported by:

    This study was supported by the Key Project of Science and Technology of Henan Province (141100110600), China Agriculture Research System of Peanut (CARS-14), Henan Province Agriculture Research System (S2012-5), the Special Fund for Independent Innovation of Henan Academy of Agriculture Science.

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摘要：

利用14个SSR标记构建了河南省2015年之前选育并审定的90个花生品种的DNA指纹图谱，用14个SSR标记产生的95个多态性位点可将90个花生品种完全区分开，其中84个品种间有≥2个位点的差异，在剩余的3对品种中，每对仅有1个差异位点。聚类分析结果表明，在遗传相似系数0.98处，90个花生品种被聚集成88类，有2对品种分别聚集在一起，是由于它们每一个品种分别以另一个品种作亲本选育而成，仅有1个差异SSR位点，表明所构建的指纹图谱是有效的。以遗传相似系数0.95为划分标准，有74.4%的品种具有特异性，与其他作物相比，河南省育成花生品种总体上亲缘关系相对较近。根据60个SSR标记的群体结构分析，90个花生品种可以分为3个亚群，与根据分枝开花习性和荚果类型的分类相吻合，亚群划分情况与聚类分析结果基本一致。

关键词: 花生品种, 指纹图谱, SSR标记, 群体结构

Abstract:

The DNA fingerprints of 90 peanut varieties released in Henan Province before 2015 were generated using 95 polymorphic loci of 14 SSR markers. All of the varieties could be distinguished by the DNA polymorphisms except for three pairs of varieties with only one SSR locus difference between each pair. SSR clustering analysis revealed that the 90 peanut varieties could be classified into 88 types at a 0.98 genetic similarity coefficient. The remaining two variety pairs had only one allele difference since one variety was the maternal parent of the other. About three fourth (74.4%) of the peanut varieties were distinctive from other varieties at a genetic similarity coefficient threshold of 0.95, indicating a very relatively narrow genetic background of the released peanut varieties in Henan Province compared with other crops. Population structure analysis based on 60 SSR markers demonstrated that the 90 varieties could be divided into three subgroups in accordance with the classification according to botanical and pod characteristics，which was consistent with the results of SSR markers cluster analysis. This study provides some fundamental information for selection of parental lines, identification and evaluation of new varieties, as well as establishment of DNA fingerprinting standard in peanut breeding program.

Key words: Peanut variety, Fingerprinting, SSR, Population structure

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