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作物学报 ›› 2017, Vol. 43 ›› Issue (01): 112-121.doi: 10.3724/SP.J.1006.2017.00112

• 耕作栽培·生理生化 • 上一篇    下一篇

绿豆品种资源萌发期耐碱性鉴定

徐宁,陈冰嬬,王明海,包淑英,王桂芳,郭中校   

  1. 吉林省农业科学院作物资源研究所,吉林公主岭 136100
  • 收稿日期:2016-04-06 修回日期:2016-07-11 出版日期:2017-01-12 网络出版日期:2016-08-01
  • 通讯作者: 郭中校, E-mail: guozhx@cjaas.com
  • 基金资助:

    本研究由国家科技支撑计划项目(2014BAD07B05-12), 国家现代农业产业技术体系建设专项(CARS-09-Z9), 吉林省农业科技创新工程(2014-1138)和吉林省育种专项资金资助(2014-65)。

Identification of Alkali Tolerance of Mungbean Germplasm Resources during Germination

XU Ning,CHEN Bing-Xu,WANG Ming-Hai,BAO Shu-Ying,WANG Gui-Fang,GUO Zhong-Xiao?   

  1. Institute of Crop Germplasm Resources, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China
  • Received:2016-04-06 Revised:2016-07-11 Published:2017-01-12 Published online:2016-08-01
  • Contact: 郭中校, E-mail: guozhx@cjaas.com
  • Supported by:

    This study was supported by the National Key Technology Support Program of China (2014BAD07B05-12), the China Agriculture Research System (CARS-09-Z9), the Agricultural Science and Technology Innovation Program of Jilin Province (2014-1138), and the Breeding Program of Jilin Province (2014-65).

摘要:

采用人工气候箱内培养皿培养,以混合碱NaHCO3∶Na2CO3 (摩尔比)为9∶1模拟典型东北地区碱胁迫环境,在萌发期以50 mmol L–1溶液处理34份绿豆品种资源,蒸馏水处理为对照,每培养皿放30粒种子。培养第3天测定发芽势,第7天测定发芽率、下胚轴长、胚根长、下胚轴干重、胚根干重等指标,通过隶属函数法和聚类分析对参试材料耐碱性综合评价,并进行因子分析。利用隶属函数法对参试材料耐碱性排序表明,不同绿豆品种资源间表现出较大差异,聚类分析把参试材料按耐碱性强弱分为4大类,白绿11等9份材料为耐碱类型,公绿1号等19份材料为耐碱中间类型,吉绿3号等5份材料为碱敏感类型,潍绿7号为碱极敏感类型。因子分析结果表明,萌发指数、下胚轴干重、胚根长分别在萌发因子、生物量累积因子和伸长因子中的负荷量最大,可作为绿豆萌发期耐碱性鉴定的适宜指标。

关键词: 绿豆, 萌发期, 耐碱性, 隶属函数, 聚类分析, 因子分析

Abstract:

Mixed alkali (NaHCO3 : Na2CO3 with the mole ratio of 9 : 1) was used to simulate the typical alkali stress environment in Northeast China. The seeds of 34 accessions of mungbean germplasm resources were treated with 50 mmol L–1 solution with distilled water as control and germinated in petri dishes for seven days. The germinating energy was measured on the third day and the germination rate, hypocotyl length, radicle length, hypocotyl dry weight, and radicle dry weight were measured on the seventh day. Through the subordinative function analysis, clustering analysis, and factor analysis, the alkali tolerance of 34 mungbean materials were evaluated. All of the materials based on subordinative function were clustered into four groups: nine accessions were tolerant to alkali, e.g. Bailyu 11, 19 accessions had midium tolerance, e.g. Gonglyu 1, five accessions were sensitive to alkali, e.g. Jilyu 3, and Weilyu 7 was strongly sensitive to alkali. The vigor index, hypocotyl dry weight, and radicle length were the most significant indexes which are recommended as the suitable indexes for identifying alkali tolerance of mungbean at germination. The results lay a foundation for alkali tolerance gene mining and variety improvement in mungbean.

Key words: Mungbean, Germination, Alkali tolerance, Subordinative function analysis, Cluster analysis, Factor analysis

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