作物学报 ›› 2017, Vol. 43 ›› Issue (05): 718-729.doi: 10.3724/SP.J.1006.2017.00718
段绍光,金黎平*,李广存,卞春松,徐建飞,胡军,屈冬玉
DUAN Shao-Guang,JIN Li-Ping*,LI Guang-Cun,BIAN Chun-Song,XU Jian-Fei,HU Jun,QU Dong-Yu
摘要:
基于16个田间表型性状对454份马铃薯材料进行UPGMA聚类分析表明,在欧氏距离14.66处所有参试材料可以被聚成2个类群A1和A。在欧氏距离12.74处类群A1又可以被分为2个亚群A11和A12。在欧氏距离11.73处全部参试材料可以被划成9个类群,包括4个小类(A、B、C和H)和五个大类(D、E、F、G和I),其中类群I所包括的材料占总数的57.5%,该结果可较准确地揭示马铃薯不同材料之间的形态差异,区分生态类型不同和遗传差异明显的亲本。利用SSR标记对559份国内外不同来源的马铃薯栽培品种进行遗传多样性分析发现,用36对多态性引物共可检测出134个多态性位点,每对引物的等位变异为1~7个,平均为3.72个,引物多态性信息量(PIC)为0.1545~0.7743,平均为0.5783,说明品种间较丰富的遗传多样性。同时,NJ系统进化树分析表明,559份马铃薯栽培种材料可分为3个大群。类群I为一个混合群,各地区品种均有分布,包括133份马铃薯材料,占总数的23.8%;类群II中欧洲、北美及中国东北和西北地区的材料所占比重较大,数量为187,占33.5%;类群III中北美、南美以及中国东北和西南地区马铃薯材料所占比重较大,包含239份材料,占42.8%。表型性状聚类与SSR分子标记聚类结果相似,均与地理位置有很大相关性,应结合共同用于评价马铃薯品种遗传多样性。
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