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作物学报 ›› 2018, Vol. 44 ›› Issue (7): 1010-1020.doi: 10.3724/SP.J.1006.2018.01010
杨文娟,张艳欣,王林海,魏鑫,黎冬华,高媛,刘盼,张秀荣(
)
Wen-Juan YANG,Yan-Xin ZHANG,Lin-Hai WANG,Xin WEI,Dong-Hua LI,Yuan GAO,Pan LIU,Xiu-Rong ZHANG()
摘要：
构建并研究应用核心种质是深入挖掘芝麻优异基因和提高育种效率的有效途径。经过对核心种质农艺、形态等性状4年4点的观察统计, 构建了包括大粒、高木酚素、高油等23种应用型在内的131份芝麻应用核心种质。为了准确鉴别这批应用核心种质并对芝麻DNA分子身份证的构建方法进行探索, 本研究利用SSR变性聚丙烯酰胺凝胶电泳技术, 从基于芝麻全基因组开发的32对SSR引物中, 筛选出7对核心引物, 进而采用SSR荧光标记毛细管电泳技术, 共检测出53个多态性位点, 最多一个引物检测到12个多态性位点。将毛细管电泳得到的分子量数据以数字+英文字母方式编码, 用ID Analysis 4.0软件根据最少引物区分最多种质的原则选核心引物中的6对(ZMM1494、ZMM1648、ZMM3037、ZMM2818、ZMM1851、ZMM1935)组合, 构建出如“4A32645(AC017)”这种简单、易使用的字符串DNA分子身份证, 并确定了不同应用型的组内引物组合。还对131份应用核心种质构建了条形码和二维码DNA分子身份证, 可迅速被电子设备识别, 拓宽了DNA分子身份证的使用范围, 并为以后芝麻种质标准化和品种DNA分子身份证库的构建奠定了重要的技术基础。
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