作物学报 ›› 2018, Vol. 44 ›› Issue (7): 1000-1009.doi: 10.3724/SP.J.1006.2018.01000
杨瑞娟1,2,白建荣2,*(),闫蕾2,苏亮2,王秀红3,李锐2,张丛卓2
Rui-Juan YANG1,2,Jian-Rong BAI2,*(),Lei YAN2,Liang SU2,Xiu-Hong WANG3,Rui LI2,Cong-Zhuo ZHANG2
摘要:
玉米ZmPHR1基因是转录因子MYB-CC家族成员, 过量表达该基因可促进低磷环境中植物对磷的吸收并改善植物的生长。本研究从磷高效利用型玉米自交系478中获得了ZmPHR1基因的5°上游序列P2205-ZmPHR1, 序列长度2205 bp。将P2205-ZmPHR1及它的4种5°端缺失启动子片段与报告基因GUS连接后转化拟南芥。GUS组织化学染色与GUS定量分析结果表明, 在0~1502 bp的片段范围内, 随着片段长度的增加, 它对外界低磷环境的响应逐步增加, 在-1502 bp时, 响应时间短, 表达活性高, 持续时间长; 超过1502 bp其活性不但没有增高, 反而变为下降或无变化。推测0至-972 bp为启动子功能区段, -972 bp至-1502 bp为启动功能增强区段。转P1502-ZmPHR1拟南芥的实时定量PCR结果显示, P1502-ZmPHR1既受外界信号的刺激, 也受体内基因产物的影响, 有反馈调节的作用, 是一个受低磷胁迫诱导的强诱导型启动子, 它诱导基因表达的功能受体内外磷浓度的调节。该研究为在低磷环境改良作物提供了理想的启动子和应用依据。
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