作物学报 ›› 2018, Vol. 44 ›› Issue (9): 1290-1300.doi: 10.3724/SP.J.1006.2018.01290
李红丹1,2,闫蕾1,2,孙蕾1,2,樊晓聪1,3,陈士瞻1,3,张燕1,3,郭林1,游光霞1,李庄1,2,杨宗举1,2,苏亮1,*,杨建平1,3,*
Hong-Dan LI1,2,Lei YAN1,2,Lei SUN1,2,Xiao-Cong FAN1,3,Shi-Zhan CHEN1,3,Yan ZHANG1,3,Lin GUO1,Guang-Xia YOU1,Zhuang LI1,2,Zong-Ju YANG1,2,Liang SU1,*,Jian-Ping YANG1,3,*
摘要:
玉米株高、开花期、产量、品质等性状与环境中的光密切相关。隐花色素是一类蓝光和近紫外光的受体, 主要参与植物的光形态建成及动、植物的生物钟调控。通过研究玉米隐花色素基因对不同光处理的表达模式, 可为进一步研究其对玉米光形态建成的作用奠定基础。本研究采用RT-PCR技术克隆了玉米ZmCRY1b和ZmCRY2基因; 利用生物信息学相关网站和软件对其编码蛋白的结构域及氨基酸进行了系统发育分析; 利用qRT-PCR分析了玉米自交系B73中ZmCRY1b和ZmCRY2基因在不同组织、以及响应不同光质及长日照和短日照处理的转录丰度。研究发现, 玉米与拟南芥、水稻和小麦的CRY蛋白有相同的结构域及较高的氨基酸序列的一致性, 表明它们具有相似的功能。ZmCRY1b和ZmCRY2基因主要在玉米的叶片中表达; 二者能迅速响应各种持续光质、黑暗到不同光质转换及长日照和短日照处理, 且ZmCRY1b在各种处理下的转录丰度均高于ZmCRY2, 可能暗示ZmCRY1b在玉米中功能更强。以上研究结果表明, ZmCRY1b和ZmCRY2基因均能有效地响应各种光质和光周期处理, 并在玉米的光形态建成中发挥重要作用。本研究为进一步探明ZmCRY1b和ZmCRY2基因的功能及其在玉米品种改良中的应用提供了研究基础。
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