作物学报 ›› 2019, Vol. 45 ›› Issue (2): 225-234.doi: 10.3724/SP.J.1006.2019.083033
牟碧涛1,2,赵卓凡1,岳灵1,李川1,张钧3,李章波3,申汉3,曹墨菊1,*()
Bi-Tao MOU1,2,Zhuo-Fan ZHAO1,Ling YUE1,Chuan LI1,Jun ZHANG3,Zhang-Bo LI3,Han SHEN3,Mo-Ju CAO1,*()
摘要:
为了发掘更多玉米C型不育胞质的强恢复系资源, 本研究对2份自交系Z16和7250-14-1进行了恢复能力的测定、恢复基因的遗传分析及恢复基因的分子标记定位。结果表明, Z16和7250-14-1对C黄早四、C478、C698-3和CMo17均表现为育性恢复, 而对C48-2则均表现为育性部分恢复。通过对玉米CMS-C不同亚组胞质测交鉴定发现, Z16对G48-2、EC48-2、ES48-2、RB48-2及类48-2均表现为不育性保持, 而7250-14-1对G48-2、EC48-2、ES48-2表现为育性部分恢复, 对RB48-2和类48-2则表现为不育性保持。Z16和7250-14-1对CMS-T不育系均表现为不育性保持, 而对CMS-S不育系则均表现为育性部分恢复。遗传分析显示, Z16对C478和C黄早四的育性恢复均受1对基因控制; 而7250-14-1对C黄早四及C478的育性恢复分别受1对基因及2对基因控制。利用(C黄早四×Z16)F2、(C黄早四×7250-14-1)F2群体分别对恢复基因进行分子标记定位, 其中Z16的恢复基因被定位于标记B-1至第8染色体短臂末端区域, 物理距离为494 kb; 7250-14-1的恢复基因被定位于第8染色体短臂的标记B-1和Chr8-86080之间, 物理距离为249 kb。该研究不仅为玉米CMS-C“三系”配套的生产利用提供了恢复基因资源, 也为玉米CMS-C恢复基因的克隆及恢复机制的研究奠定了一定基础。
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