作物学报 ›› 2019, Vol. 45 ›› Issue (2): 235-247.doi: 10.3724/SP.J.1006.2019.84063
邹雪1,2,丁凡1,余金龙1,彭洁2,邓孟胜2,王宇2,刘丽芳1,余韩开宗1,陈年伟1,王西瑶2,*()
Xue ZOU1,2,Fan DING1,Jin-Long YU1,Jie PENG2,Meng-Sheng DENG2,Yu WANG2,Li-Fang LIU1,Kai-Zong YU-HAN1,Nian-Wei CHEN1,Xi-Yao WANG2,*()
摘要:
马铃薯块茎过早萌芽会降低商品价值, 本试验比较挥发性物质的抑芽效应, 并从转录和蛋白水平分析其作用机制。结果表明, 抑芽能力薄荷醇?樟脑?萘, 萌芽受抑降低代谢消耗, 薄荷醇处理180 d时的重量损失只有对照损失的36%。樟脑处理萌芽块茎3 d引起表达显著上调(或下调)的基因和蛋白分别有1227 (299)和296 (204)个, 主要参与响应刺激、防御反应。贮藏期间, 果胶代谢基因PEL、PME、PG, 角质合成基因CYP77A6、HPFT、WES, 乙烯合成基因ACO以及转录因子编码基因GATA4L的表达量随时间升高。樟脑早期不同程度地刺激这些基因表达, 中后期则抑制, 49 d时只有对照的0.68%~23.35%。薄荷醇使上述基因表达保持低水平, 但可提高细胞周期负控基因KRP4的表达, 为对照的15.9倍。植物病原菌互作通路中的基因WRKY75、STH-2、RBOH表达受樟脑诱导并在后期高表达。樟脑和薄荷醇均能抑制生长发育基因的表达, 造成芽死亡, 降低贮藏损耗。前者早期能促进合成保护性物质, 萌芽时有更强烈的抗菌反应; 后者则阻碍细胞分裂。
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