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作物学报 ›› 2020, Vol. 46 ›› Issue (02): 228-237.doi: 10.3724/SP.J.1006.2020.92032

• 耕作栽培·生理生化 • 上一篇    下一篇

磷营养对水稻籽粒锌生物有效性的影响及其与植酸等磷酸肌醇谱含量的关系

苏达1,2,吴良泉2,Søren K. Rasmussen3,周庐建4,潘刚4,程方民4,*()   

  1. 1 福建农林大学农学院 / 作物遗传育种与综合利用教育部重点实验室, 福建福州 350002
    2 福建农林大学国际镁营养研究所, 福建福州 350002
    3 哥本哈根大学植物与环境科学系, 丹麦哥本哈根
    4 浙江大学农业与生物技术学院, 浙江杭州 310058
  • 收稿日期:2019-06-03 接受日期:2019-08-09 出版日期:2020-02-12 网络出版日期:2019-09-10
  • 通讯作者: 程方民
  • 作者简介:E-mail: littlepest@126.com, Tel: 0591-83722796
  • 基金资助:
    本研究由国家重点研发计划项目(2016YFD0300502);国家自然科学基金项目(31571602);福建省自然科学基金项目(2019J01374);福建省中青年教师教育科研项目(JAT170156);国家留学基金委项目资助

Influence of phosphorus on rice (Oryza sativa L.) grain zinc bioavailability and its relation to inositol phosphate profiles concentration

SU Da1,2,WU Liang-Quan2,K. Rasmussen Søren3,ZHOU Lu-Jian4,PAN Gang4,CHENG Fang-Min4,*()   

  1. 1 Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops / College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
    2 International Magnesium Institute, Fuzhou 350002, Fujian, China
    3 Department of Plant and Environmental Sciences, Section of Plant and Soil Science, University of Copenhagen, Copenhagen, Denmark
    4 College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China
  • Received:2019-06-03 Accepted:2019-08-09 Published:2020-02-12 Published online:2019-09-10
  • Contact: Fang-Min CHENG
  • Supported by:
    This study was supported by the National Key Research and Development Project(2016YFD0300502);the National Natural Science Foundation of China(31571602);the Fujian Province Natural Science(2019J01374);the Education and Scientific Research Project for Middle-aged and the Young Teachers in Fujian Province(JAT170156);the Foundation for China Scholarship Council

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摘要：

作物籽粒锌缺乏所导致的“隐性饥饿”已威胁到全世界1/3人群的健康。为明确磷对水稻籽粒锌的生物有效性的影响, 利用大田磷肥长期定位试验, 并结合稻穗离体培养技术, 探讨了不同外源磷浓度下水稻籽粒锌的生物有效性的变化及其与籽粒植酸等磷酸肌醇谱含量的关系。试验表明高磷处理显著增加水稻籽粒中植酸磷和总磷的含量, 以及不同价位磷酸肌醇(InsP1-6)含量, 其中以高价磷酸肌醇(InsP4-6)在磷供给下的增幅最为显著。与籽粒植酸的变化趋势相反, 高磷水平降低了稻米中锌的含量及其有效性。稻穗离体培养中, 高磷(P12)处理相比无磷(P0)对照, 籽粒锌的有效性降幅高达81.3%。因此, 过量磷肥投入会通过显著提高水稻籽粒中的植酸及高价磷酸肌醇含量, 在同步降低籽粒锌含量的同时, 进一步显著降低其在水稻籽粒中的生物有效性。

关键词: 磷, 植酸, 锌生物有效性, 磷酸肌醇, 稻米品质

Abstract:

The hidden hunger caused by grain zinc (Zn) deficiency in crop poses a potential threat to the health of nearly two billion people worldwide, especially in developing countries. In this study, the long-term phosphorus fertilizer experiment and in vitro detached rice panicle culture systems with varied phosphorus levels were conducted to investigate the effect of phosphorus on rice grain Zn bioavailability and its relation of grain inositol phosphates profiles (phytic acid related metabolic derivatives) concentration. In our results, compared with low phosphorus level, high phosphorus supply increased grain phytic acid phosphorus and total phosphorus concentration (mg g -1). Moreover, high phosphorus supply also increased different grain inositol phosphate profile concentrations (InsP1-6), especially for InsP4-6. On the contrary, grain Zn concentration decreased with phosphorus supply. Both the decrement of Zn and increment of phytic acid phosphorus induced by the higher phosphorus supply in rice grain led to the significant decrement of grain Zn bioavailability. In in vitro detached panicle culture system, the Zn bioavailability in P12 treatment decreased by 81.3% relative to P0 treatment. In conclusion, higher phosphorus input could significantly decrease grain Zn bioavailability through increased grain phytic acid phosphorus and inositol phosphates derivatives concentration, in addition to the decrement of grain Zn concentration.

Key words: phosphorus, phytic acid, zinc bioavailability, inositol phosphate, rice quality

图1

大田磷肥处理对水稻籽粒植酸磷、总磷含量以及植酸磷/总磷比值的影响 标以相同小写字母的柱值差异未达到0.05的显著水平。LP: 低磷处理; MP: 中磷处理; HP: 高磷处理; PAP: 植酸磷; Total P: 总磷; PAP/total P: 植酸磷/总磷。"

图2

大田磷肥处理对水稻籽粒锌、铁、锰、铜含量以及锌的生物有效性的影响 标以相同小写字母的柱值差异未达到0.05的显著水平。LP: 低磷处理; MP: 中磷处理; HP: 高磷处理。TAZ: 锌的生物有效性。"

图3

外源磷处理对水稻籽粒植酸磷、总磷含量以及植酸磷/总磷的比值的影响(稻穗离体培养) 标以相同小写字母的柱值差异未达到0.05的显著水平。P0、P1、P3、P6、P12分别代表0、1、3、6和12 mmol L-1的5个NaH2PO4·2H2O浓度梯度处理。PAP: 植酸磷; Total P: 总磷; PAP/total P: 植酸磷/总磷。"

图4

稻穗离体培养体系中不同磷浓度水平下水稻籽粒中的磷分布(5000倍光镜下稻米横断面扫描, 100 μm) A: P0处理; B: P3处理; C: P12处理。"

图5

外源磷处理对水稻籽粒不同价位磷酸肌醇含量的影响(稻穗离体培养) P0、P1、P3、P6、P12分别代表0、1、3、6和12 mmol L-1的5个NaH2PO4·2H2O浓度梯度处理。"

图6

外源磷处理下水稻籽粒中不同价位磷酸肌醇占总磷酸肌醇含量的比例(稻穗离体培养)"

图7

稻穗离体培养体系中不同磷浓度水平下水稻籽粒中锌的分布(5000倍光镜下稻米横断面扫描, 标尺为100 μm) A: P0处理; B: P3处理; C: P12处理。"

图8

外源磷处理水稻籽粒锌的含量及其有效性的影响(稻穗离体培养) 标以相同小写字母的柱值差异未达到0.05的显著水平。P0、P1、P3、P6、P12分别代表0、1、3、6和12 mmol L-1的5个NaH2PO4·2H2O浓度梯度处理。TAZ: 锌的生物有效性。"

图9

不同磷肥处理水平下水稻籽粒磷组分和锌的生物有效性之间的相关性分析(左: 大田试验; 右: 大田试验+稻穗离体培养试验) PAP: 植酸磷; TP: 总磷; TAF: 锌的生物有效性。"

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