作物学报 ›› 2020, Vol. 46 ›› Issue (7): 1025-1032.doi: 10.3724/SP.J.1006.2020.94152
LI Guo-Ji**,ZHU Lin**,CAO Jin-Shan,WANG You-Ning()
摘要:
拟南芥中硝酸盐的吸收、转运和分配是通过硝酸盐转运蛋白(nitrate transporter, NRT)实现的。尽管之前的生物信息学分析推测大豆GmNRT1.2s可能参与共生固氮过程, 但尚未开展相应的功能研究。本研究通过对其表达模式分析发现, GmNRT1.2a和GmNRT1.2b分别在根和叶中高表达, 且受硝酸盐诱导, 在接种根瘤菌与结瘤因子(nod factors, NFs)后表达量明显升高。功能研究结果显示, 过表达GmNRT1.2a或GmNRT1.2b后大豆根瘤数目显著增加。本研究为深入探究GmNRT1.2a和GmNRT1.2b调控大豆共生固氮过程的分子机制提供了一定的数据支持。
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