基于WGCNA的马铃薯根系抗旱相关共表达模块鉴定和核心基因发掘

doi:10.3724/SP.J.1006.2020.94130

作物学报 ›› 2020, Vol. 46 ›› Issue (7): 1033-1051.doi: 10.3724/SP.J.1006.2020.94130

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

基于WGCNA的马铃薯根系抗旱相关共表达模块鉴定和核心基因发掘

秦天元^1,^2,^**,孙超^1,^2,^**,毕真真^1,²,梁文君^1,²,李鹏程^1,²,张俊莲¹,白江平^1,^2,^*()

¹ 甘肃省干旱生境作物学重点实验室 / 甘肃省作物遗传改良与栽培种创新重点实验室, 甘肃兰州 730070
² 甘肃农业大学农学院, 甘肃兰州 730070

收稿日期:2019-08-27 接受日期:2019-12-26 出版日期:2020-07-12 网络出版日期:2020-01-14
通讯作者: 白江平
作者简介:秦天元, E-mail: 1637835362@qq.com|孙超, E-mail: sunc@gsau.edu.cn
^** 同等贡献
基金资助:
国家自然科学基金项目(31660432);国家自然科学基金项目(31460369);国家现代农业产业技术体系(马铃薯)建设专项(CARS-09-P14);甘肃省马铃薯产业体系(GARS-03-P1);中国科学院“西部之光”人才培养计划(2014-01);兰州市科技发展计划(2015-3-62);甘肃省教育厅项目(2019B-073);甘肃省自然科学基金(18JR3RA174);甘肃农业大学国重实验室开放基金(GSCS-2017-9);甘肃农业大学创新基金(GAU-XKJS-2018-085)

Identification of drought-related co-expression modules and hub genes in potato roots based on WGCNA

Tian-Yuan QIN^1,^2,^**,Chao SUN^1,^2,^**,Zhen-Zhen BI^1,²,Wen-Jun LIANG^1,²,Peng-Cheng LI^1,²,Jun-Lian ZHANG¹,Jiang-Ping BAI^1,^2,^*()

¹ Gansu Provincial Key Laboratory of Aridland Crop Science / Gansu Key Laboratory of Crop Improvement & Germplasm Enhancement, Lanzhou 730070, Gansu, China;
² College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu, China

Received:2019-08-27 Accepted:2019-12-26 Published:2020-07-12 Published online:2020-01-14
Contact: Jiang-Ping BAI
About author:^** Contributed equally to this work
Supported by:
National Natural Science Foundation of China(31660432);National Natural Science Foundation of China(31460369);China Agricultural Research System (Potato)(CARS-09-P14);Gansu Agricultural Research System (Potato)(GARS-03-P1);“Light of the West” Talent Training Program of the Chinese Academy of Sciences(2014-01);Lanzhou Science and Technology Development Plan(2015-3-62);Gansu Provincial Department of Education(2019B-073);Gansu Science and Technology Fund(18JR3RA174);Gansu Provincial Key Laboratory of Aridland Crop Science, GAU(GSCS-2017-9);GAU Fund(GAU-XKJS-2018-085)

摘要/Abstract

摘要：

权重基因共表达网络分析(Weighted Gene Co-expression Network Analysis, WGCNA)是系统生物学的一种研究方法, 在多样本转录组数据中挖掘与目标性状相关的基因模块有较广泛的应用。为了深入探究马铃薯应对干旱胁迫的分子机制, 本研究以国际马铃薯中心引进栽培种C16 (CIP 397077.16)和C119 (CIP 398098.119)为试验材料, 将其无菌组培苗利用甘露醇模拟干旱胁迫, 处理0 h、2 h、6 h、12 h和24 h, 取其根系进行转录组测序, 每样设3个生物学重复, 共30个样本。基于以上转录组数据, 利用WGCNA构建与抗逆生理性状相关联的权重基因共表达网络, 得到15个与根系抗旱密切相关的基因共表达模块, 并从4个与目标性状关联度最高的模块中发掘到数个核心基因, 功能注释表明其中大部分参与干旱胁迫调控通路。这些结果为进一步研究马铃薯根系抗旱的分子遗传机制提供了线索。

关键词: 马铃薯, 干旱胁迫, 权重基因共表达网络, 转录组学

Abstract:

Weighted gene co-expression network analysis (WGCNA) is a research method in systematic biology. It is widely used to identify gene modules related to target traits in multi-sample transcriptome data. In order to further explore the molecular mechanism of potato response to drought stress, two cultivars (C16: CIP 397077.16 and C119: CIP 398098.119) introduced from the International Potato Center were used as experimental materials, and five drought stress gradients were treated for 0 h, 2 h, 6 h, 12 h, and 24 h, with the untreated material as a control. A total of 30 samples of root system were used for transcriptome sequencing, with three biological replicates. Based on the above transcriptome data, we constructed a co-expression network of weighted genes associated with stress-resistant physiological traits by WGCNA, and obtained 15 gene co-expression modules closely related to root drought resistance. In addition, a number of hub genes involved in drought stress regulation pathways were discovered from the four modules with the highest correlation with target traits. These results provide clues for further study on the molecular genetic mechanisms of potato root drought resistance.

Key words: potato, drought stress, weighted gene co-expression network, transcriptome

秦天元, 孙超, 毕真真, 梁文君, 李鹏程, 张俊莲, 白江平. 基于WGCNA的马铃薯根系抗旱相关共表达模块鉴定和核心基因发掘[J]. 作物学报, 2020, 46(7): 1033-1051.

QIN Tian-Yuan, SUN Chao, BI Zhen-Zhen, LIANG Wen-Jun, LI Peng-Cheng, ZHANG Jun-Lian, BAI Jiang-Ping. Identification of drought-related co-expression modules and hub genes in potato roots based on WGCNA[J]. Acta Agronomica Sinica, 2020, 46(7): 1033-1051.

图/表 17

表1

图1

附表 1

图2

图3

图4

图5

图6

图7

图8

图9

表2

目标模块的部分GO富集分析结果"

模块 Module	GO条目 GO term	基因本体 Ontology	描述 Description	P值 P-value
Red	GO:0016705	F	氧化还原酶活性, 作用于成对供体, 与分子氧结合或还原。 Oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen.	2.90E-09
Red	GO:0004866	F	内肽酶抑制剂活性。 Endopeptidase inhibitor activity.	0.00062
Red	GO:0030414	F	肽酶抑制剂活性。 Peptidase inhibitor activity.	0.00062
Red	GO:0005515	F	蛋白质结合。 Protein binding.	0.0012
Yellow	GO:0005515	F	蛋白质结合。 Protein binding.	2.20E-13
Turquoise	GO:0015979	P	光合作用。 Photosynthesis.	7.10E-07
模块 Module	GO条目 GO term	基因本体 Ontology	描述 Description	P值 P-value
Turquoise	GO:0019684	P	光合作用, 光反应。 Photosynthesis, light reaction.	5.90E-06
Turquoise	GO:0006694	P	类固醇生物合成过程。 Steroid biosynthetic process.	5.60E-05
Turquoise	GO:0010033	P	对有机物质的反应。 Response to organic substance.	0.00023
Turquoise	GO:0005515	F	蛋白结合。 Protein binding.	4.00E-27
Turquoise	GO:0016705	F	氧化还原酶活性, 作用于成对供体, 与分子氧结合或还原。 Oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen.	3.00E-07
Turquoise	GO:0003854	F	3-β-羟基-Δ5-类固醇脱氢酶活性。 3-beta-hydroxy-delta5-steroid dehydrogenase activity.	3.30E-05
Turquoise	GO:0033764	F	类固醇脱氢酶活性, 作用于供体的羟基, NAD或NADP作为受体。 Steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor.	3.30E-05
Turquoise	GO:0016229	F	类固醇脱氢酶活性。 Steroid dehydrogenase activity.	3.30E-05
Turquoise	GO:0016614	F	氧化还原酶活性, 作用于供体的羟基。 Ooxidoreductase activity, acting on CH-OH group of donors.	7.10E-05
Turquoise	GO:0016616	F	氧化还原酶活性, 作用于供体的羟基, NAD或NADP作为受体。 Oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor.	0.00026
Turquoise	GO:0051287	F	NAD或NADH结合。 NAD or NADH binding.	0.00039
Turquoise	GO:0008092	F	细胞骨架蛋白结合。 Cytoskeletal protein binding.	0.00062
Blue	GO:0007010	P	细胞骨架组织。 Cytoskeleton organization.	1.20E-05
Blue	GO:0005515	F	蛋白结合。 Protein binding.	2.70E-29
Blue	GO:0016705	F	氧化还原酶活性, 作用于成对供体, 与分子氧结合或还原。 Oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen.	2.50E-10
Blue	GO:0016021	C	膜结合。 Integral to membrane.	7.30E-05
Blue	GO:0015629	C	肌动蛋白细胞骨架。 Actin cytoskeleton.	0.00021
Blue	GO:0031224	C	膜固有的。 Intrinsic to membrane.	0.00036

表2

图10

图11

表3

不同模块中核心基因的功能注释"

模块 Module	核心基因 Hub gene	核心基因在拟南芥同源基因 Hub gene in A. thaliana	基因功能 Gene function
Red	PGSC0003DMG400030368	AT1G07140	编码假定的RAN结合蛋白。 Encodes a putative RAN-binding protein (siRANBP).
Red	PGSC0003DMG400003662	AT1G20270; AT5G66060	2-氧戊二酸和铁依赖性加氧酶超家族蛋白; 铁离子结合/氧化还原酶/氧化还原酶蛋白。 2-oxoglutarate and Fe-dependent oxygenase superfamily protein; Ironion binding/oxidoreductase/oxidoreductase protein.
Red	PGSC0003DMG402020462	AT5G25360	假设性蛋白质。 Hypothetical protein (source: Araport 11).
Red	PGSC0003DMG400005154	X	未知功能。Unknown.
Red	PGSC0003DMG400020562	AT2G40470	含LOB结构域的蛋白质。参与木质部分化的调节——作为VND7的调节器, VND7是木质部细胞分化的主要调节器。 LOB-domain containing protein. Involved in regulation of xylem differentiation—acts as a regulator of VND7 which is a master regulator of xylem cell differentiation.
Yellow	PGSC0003DMG400029350	AT1G05680^[15]	编码一个UDP-葡萄糖基转移酶, ugt74e2, 作用于IBA (吲哚-3-丁酸)并影响生长素的稳态。这种酶的转录和蛋白质水平被H₂O₂强烈诱导, 可能允许ROS (活性氧物种)和生长素信号的整合。 Encodes a UDP-glucosyltransferase, ugt74e2, that acts on IBA (indole-3-butyric acid) and affects auxin homeostasis. The transcript and protein levels of this enzyme are strongly induced by H₂O₂ and mayallow integration of ROS (reactive oxygen species) and auxin signaling.
Yellow	PGSC0003DMG400024717	AT3G05545	超家族蛋白。 RING/U-box superfamily protein (source: Araport 11).
Yellow	PGSC0003DMG400026572	AT3G51730^[16]	含皂苷B结构域的蛋白质。 Saposin B domain-containing protein (source: Araport 11).
Yellow	PGSC0003DMG400006704	AT5G35200^[17]	超家族蛋白。 ENTH/ANTH/VHS superfamily protein (source: Araport 11).
Yellow	PGSC0003DMG400015386	AT2G42610	光依赖性短下胚轴样蛋白。 LIGHT-DEPENDENT SHORT HYPOCOTYLS-like protein (DUF640) (source: Araport 11).
Yellow	PGSC0003DMG400026238	AT3G60410	假设性蛋白质。 Hypothetical protein (DUF1639) (source: Araport 11).
模块 Module	核心基因 Hub gene	核心基因在拟南芥同源基因 Hub gene in A. thaliana	基因功能 Gene function
Yellow	PGSC0003DMG400020683	AT3G25910	MAP激酶 Mitogen-activated protein kinases (DUF1644)(source: Araport 11).
Yellow	PGSC0003DMG400024687	AT1G55000^[18]	含蛋白的肽聚糖结合赖氨酸域。 Peptidoglycan-binding LysM domain-containing protein (source: Araport 11).
Yellow	PGSC0003DMG400006516	AT1G55570	SKU5 similar 12 (source: Araport 11).
Turquoise	PGSC0003DMG400013391	AT3G22990	含有蛋白质的犰狳重复序列, 位于核内, 广泛表达于植物。 Armadillo-repeat containing protein, located in nucleus, broadly expressed throughout vegetative.
Turquoise	PGSC0003DMG400003792	AT3G14110^[19]	编码一种新的盘绕线圈, 含有TPR结构域的蛋白质, 定位于叶绿体膜并参与叶绿素的生物合成。 Encodes a novel coiled-coil, TPR domain containing protein that is localized to the chloroplast membrane and is involved in chlorophyll biosynthesis.
Turquoise	PGSC0003DMG400024698	AT5G27620	核心细胞周期基因的mRNA是细胞间流动的。 Core cell cycle genes the mRNA is cell-to-cell mobile.
Turquoise	PGSC0003DMG402011550	AT5G23200	C5orf35 (source: Araport 11).
Blue	PGSC0003DMG400018372	X	未知功能。Unknown.
Blue	PGSC0003DMG400026187	AT3G50950	编码一种典型的CC型NLR蛋白, 该蛋白是识别病原菌丁香中T3SE-Hopz1a所必需的。 Encodes a canonical CC-type NLR protein that is required for the recognition of the T3SE HopZ1a from the pathogenic bacteria P. syringae

表3

表4

图12

参考文献 36

[1]	赵鸿, 任丽雯, 赵福年, 齐月, 蔡迪花, 王春玲, 陈斐, 雷俊, 王润元, 王鹤龄, 张凯, 姚玉璧, 王兴. 马铃薯对土壤水分胁迫响应的研究进展. 干旱气象, 2018,36:537-543.
	Zhao H, Ren L W, Zhao F N, Qi Y, Cai D H, Wang C L, Chen F, Lei J, Wang R Y, Wang H L, Zhang K, Yao Y B, Wang X. Potato response to soil water stress research progress. Drought Meteorol, 2018,36:537-543 (in Chinese with English abstract).
[2]	李海珀. 铃薯抗旱性研究进展. 种子科技, 2018,36(3):118-120.
	Li H P. Advances in drought resistance of potatoes. Seed Sci Technol, 2018,36(3):118-120 (in Chinese with English abstract).
[3]	秦天元, 孙超, 毕真真, 王瀚, 李鑫, 曾文婕, 白江平. 物根系成像技术研究进展及马铃薯根系研究应用前景. 核农学报, 2019,33:412-419.
	Qin T Y, Sun C, Bi Z Z, Wang H, Li X, Zeng W J, Bai J P. The research progress of plant root imaging technology and the application prospect of potato root research. J Nucl Agric, 2019,33:412-419 (in Chinese with English abstract).
[4]	Clark L J, Whalley W R, Barraclough P B. How do roots penetrate strong soil. Plant Soil, 2003,255:93-104. doi: 10.1023/A:1026140122848
[5]	Liu S, Wang Z, Chen D, Zhang B, Tian R R, Wu J, Zhang Y, Xu K Y, Yang L M, Cheng C, Ma J, Lyu L B, Zheng Y T, Hu X T, Yi Z, Wang X T, Li J L. Annotation and cluster analysis of spatiotemporal- and sex-related lncRNA expression in rhesus macaque brain. Genome Res, 2017,27:1608-1620. doi: 10.1101/gr.217463.116 pmid: 28687705
[6]	Greenham K, Guadagno C R, Gehan M A, Mockler T C, Weinig C, Ewers B E, McClung C R. Temporal network analysis identifies early physiological and transcriptomic indicators of mild drought in brassica rapa. eLife, 2017,6:e29655. doi: 10.7554/eLife.29655 pmid: 28826479
[7]	Hollender C A, Kang C, Darwish O, Geretz A, Matthews B F, Slovin J, Alkharouf N, Liu Z. Floral transcriptomes in woodland strawberry uncover developing receptacle and anther gene networks. Plant Physiol, 2014,165:1062-1075. pmid: 24828307
[8]	杨宇昕, 桑志勤, 许诚, 代文双, 邹枨. 用WGCNA进行玉米花期基因共表达模块鉴定. 作物学报, 2019,45:161-174.
	Yang Y X, Sang Z Q, Xu C, Dai W S, Zou C. Identification of co-expression module of maize florescence genes using WGCNA. Acta Agron Sin, 2019,45:161-174 (in Chinese with English abstract).
[9]	Miller G, Suzuki N, Ciftci Y S. Reactive oxygen species homeostasis and signalling during drought and salinity stresses. Plant Cell Environ, 2010,33:453-467. doi: 10.1111/j.1365-3040.2009.02041.x pmid: 19712065
[10]	Wrzaczek M, Brosché M, Kangasjärvi J. ROS signaling loops-production, perception, regulation. Curr Opin Plant Biol, 2013,16:575-582. doi: 10.1016/j.pbi.2013.07.002 pmid: 23876676
[11]	Baxter A, Mittler R, Suzuki N. ROS as key players in plant stress signaling. J Exp Bot, 2014,65:1229-1240. doi: 10.1093/jxb/ert375 pmid: 24253197
[12]	Zhou D, Xin Z, Yi L, Zhen H Z, Zhen S. AgriGO: a GO analysis toolkit for the agricultural community. Nucleic Acids Res, 2010,38:64-70.
[13]	Tian T, Yue L, Heng Y Y, Qi Y, Xin Y, Zhou D, Wen Y X, Zhen S. AgriGO v 2.0: a GO analysis toolkit for the agricultural community, 2017 update. Nucleic Acids Res, 2017,45:122-129.
[14]	Shannon P, Markiel A, Ozier O, Baliga N S, Wang J T, Ramage D, Amin N, Schwikowski B, Ideker T. Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Res, 2003,13:2498-2504. pmid: 14597658
[15]	Tognetti T A, Kris M, Korneel V, Brigitte C, Inge D C, Sheila C, Ricarda F, Els P, Wout B, Bernard G, Dirk S K A, Frank V B. Perturbation of indole-3-butyric acid homeostasis by the UDP-glucosyltransferase UGT74E2 modulates Arabidopsis architecture and water stress tolerance. Plant Cell, 2010,22:2660-2679. doi: 10.1105/tpc.109.071316 pmid: 20798329
[16]	Clay C, Song Q P, Jan Z, Thomas G. The vegetative vacuole proteome of Arabidopsis thaliana reveals predicted and unexpected proteins. Plant Cell, 2004,16:3285-3303. pmid: 15539469
[17]	Benschop J J, Mohammed S, O’Flaherty M, Heck A J, Slijper M, Menke F L. Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis. Mol Cell Proteomics, 2007,6:1198-1214. doi: 10.1074/mcp.M600429-MCP200 pmid: 17317660
[18]	Gagne J M, Downes B P, Shiu S H, Durski A M, Vierstra R D. The F-box subunit of the SCF E3 complex is encoded by a diverse superfamily of genes in Arabidopsis. Proc Natl Acad Sci USA, 2002,99:11519-11524. pmid: 12169662
[19]	Laloi C, Przybyla D, Apel K. A genetic approach towards elucidating the biological activity of different reactive oxygen species in Arabidopsis thaliana, J Exp Bot, 2006,57:1719-1724. pmid: 16720605
[20]	Fedoroff N V, Battisti D S, Beachy R N, Cooper P J, Fischhoff D A, Hodges C N, Knauf V C, Lobell D, Mazur B J, Molden D Reynolds M P, Ronald P C, Rosegrant M W, Sanchez P A, Vonshak A, Zhu J K. Radically rethinking agriculture for the 21st century. Science, 2010,327:833-834. doi: 10.1126/science.1186834 pmid: 20150494
[21]	纪瑞鹏, 于文颖, 冯锐, 武晋雯, 张玉书, 王茜. 作物对干旱胁迫的响应过程与早期识别技术研究进展. 灾害学, 2019,34(2):153-160.
	Ji R P, Yu W Y, Feng R, Wu J W, Zhang Y S, Wang Q. Advances in crop response to drought stress and early identification techniques. Disaster Sci, 2019,34(2):153-160 (in Chinese with English abstract).
[22]	Yuan Q Z, Wu S H, Dai E F, Zhao D S, Ren P, Zhang X R. NPP vulnerability of the potential vegetation of China to climate change in the past and future. J Geogr Sci, 2017,27:131-142.
[23]	Huang L, Li W C, Tam N F Y, Ye Z H. Effects of root morphology and anatomy on cadmium uptake and translocation in rice (Oryza sativa L.). J Environ Sci, 2019,75:296-306.
[24]	郭宾会, 戴毅, 宋丽. 干旱下植物激素影响作物根系发育的研究进展. 生物技术通报, 2018,34(7):48-56.
	Guo B H, Dai Y, Song L. Advances in plant hormones affecting root development under drought. Biotechnol Bull, 2018,34(7):48-56 (in Chinese with English abstract).
[25]	朱维琴, 吴良欢, 陶勤南. 作物根系对干旱胁迫逆境的适应性研究进展. 土壤与环境, 2002,11:430-433.
	Zhu W Q, Wu L H, Tao Q N. Advances in research on the adaptability of crop roots to drought stress. Soil Environ, 2002,11:430-433 (in Chinese with English abstract).
[26]	Xu Z P, Miao Y X, Chen Z A, Gao H L, Wang R X, Zhao D S, Zhang B C, Zhou Y H, Tang S Z, Zhang H G, Liu Q Q. Identification and fine mapping of qGN1c, a QTL for grain number per panicle, in rice (Oryza sativa L.). Mol Breed, 2019,39:1-12. doi: 10.1007/s11032-018-0907-x
[27]	王伟伟, 王洪洋, 刘晶, 梁静思, 李灿辉, 唐唯. 马铃薯重要性状QTL定位及3个抗病性状分子标记辅助选育. 作物杂志, 2018, (6):10-16.
	Wang W W, Wang H Y, Liu J, Liang J S, Li C H, Tang W. Mapping of important traits of potato and molecular marker assisted selection of three disease resistant traits. Crops, 2018, (6):10-16 (in Chinese with English abstract).
[28]	Guo Y, Xing Y. Weighted gene co-expression network analysis of pneumocytes under exposure to a carcinogenic dose of chloroprene. Life Sci, 2016,151:339-347. doi: 10.1016/j.lfs.2016.02.074 pmid: 26916823
[29]	Tao W, Xing W H, Xin T L, Yu J L, Wen J Z, Qiang H, Wan J L, Lu Y X, Rong T, Hong J W, He S Z. Weighted gene co-expression network analysis identifies FKBP11 as a key regulator in acute aortic dissection through a NF-kB dependent pathway. Front Physiol, 2017,8:1-17. doi: 10.3389/fphys.2017.00001 pmid: 28154536
[30]	Hai T Z, Xin X D, Kai Z, Yue Z L, Yu J W, Jin X L, Yan H, Xu B W, Quan Q Z. Weighted correlation network analysis (WGCNA) of Japanese flounder (Paralichthys olivaceus) embryo transcriptome provides crucial gene sets for understanding haploid syndrome and rescue by diploidization. J Ocean Univ China, 2018,17:1441-1450. doi: 10.1007/s11802-018-3656-x
[31]	Zhao W, Langfelder P, Fuller T, Dong J, Li A, Hovarth S. Weighted gene coexpression network analysis: state of the art. J Biopharmaceutical Statistics, 2010,20:281-300.
[32]	Jaspers P, Kangasjrvi J. Reactive oxygen species in abiotic stress signaling. Physiol Plant, 2010,138:405-413. pmid: 20028478
[33]	尹智宇, 郭华春, 封永生, 肖关丽. 干旱胁迫下马铃薯生理研究进展. 中国马铃薯, 2017,31:234-239.
	Yin Z Y, Guo H C, Feng Y S, Xiao G L. Advances in potato physiology under drought stress. Chin Potato, 2017,31:234-239 (in Chinese with English abstract).
[34]	崔慧妮, 屠培培, 李晓丽, 李燕燕, 田凤龙, 张宪省, 孙庆泉. 农杆菌介导的ZmHSD1基因转入玉米自交系. 山东农业科学, 2013,45(7):6-8.
	Cui H N, Tu P P, Li X L, Li Y Y, Tian F L, Zhang X S, Sun Q Q. Agrobacterium-mediated transformation of ZmHSD1 gene into maize inbred lines. Shandong Agric Sci, 2013,45(7):6-8 (in Chinese with English abstract).
[35]	孙超, 黎家. 油菜素甾醇类激素的生物合成、信号转导和代谢. 植物生理学报, 2017,53:291-307.
	Sun C, Li J. Biosynthesis, signal transduction and metabolism of brassinosteroids. J Plant Physiol, 2017,53:291-307 (in Chinese with English abstract).
[36]	Tognetti V B, Van Aken O, Morreel K, Vandenbroucke K, van de Cotte B, De Clercq I, Chiwocha S, Fenske R, Prinsen E, Boerjan W, Genty B, Stubbs K A, Inzé D, van Breusegem F. Perturbation of indole-3-butyric acid homeostasis by the UDP-glucosyltransferase UGT74E2 modulates Arabidopsis architecture and water stress tolerance. Plant Cell, 2010,22:2660-2679. doi: 10.1105/tpc.109.071316 pmid: 20798329

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

品种 Variety	CIP编号 CIP number	母本 Maternal parent	父本 Paternal parent
C16	CIP397077.16	392025.7=(LR93.221)	392820.1=(C93.154)
C119	CIP398098.119	393371.58	392639.31

处理时间Treatment time	超氧化物歧化酶 Superoxide dismutase (U g^-1)		过氧化物酶 Peroxidase (U g^-1 min^-1)		过氧化氢酶 Catalase (U g^-1 min^-1)		根活力 Root vitality (mg g^-1 h^-1)
处理时间Treatment time	平均值 Average value	标准差 Standard deviation	平均值 Average value	标准差 Standard deviation	平均值 Average value	标准差 Standard deviation	平均值 Average value	标准差 Standard deviation
C16-0 h	9.57	2.00	2637.50	135.95	116.75	6.72	457.74	35.21
C16-2 h	14.13	1.55	3375.00	187.90	129.93	7.68	457.75	30.18
C16-6 h	15.63	1.81	3862.50	162.71	144.84	13.26	496.57	33.10
C16-12 h	16.42	1.75	4262.50	149.01	155.83	15.39	627.09	30.73
C16-24 h	22.22	1.35	8575.00	142.27	209.34	20.20	815.49	62.11
C119-0 h	46.02	5.15	1312.50	190.92	244.25	15.10	712.44	36.99
C119-2 h	48.92	6.91	3200.00	262.15	274.48	22.32	811.53	64.44
C119-6 h	49.16	6.29	3500.00	218.72	276.74	14.75	826.29	81.45
C119-12 h	56.62	7.72	3687.50	206.59	341.57	16.84	901.41	23.47
C119-24 h	75.53	8.95	6112.50	210.12	370.75	19.51	924.88	70.89

基于WGCNA的马铃薯根系抗旱相关共表达模块鉴定和核心基因发掘

Identification of drought-related co-expression modules and hub genes in potato roots based on WGCNA

RichHTML

PDF

摘要/Abstract

引用本文

使用本文

图/表 17

参考文献 36

相关文章 15

Metrics

本文评价

推荐阅读 0

关于本刊

在线期刊

作者中心

相关单位

联系我们

[1]	王海波, 应静文, 何礼, 叶文宣, 涂卫, 蔡兴奎, 宋波涛, 柳俊. rDNA和端粒重复序列鉴定马铃薯和茄子体细胞杂种染色体丢失和融合[J]. 作物学报, 2022, 48(5): 1273-1278.
[2]	石艳艳, 马志花, 吴春花, 周永瑾, 李荣. 垄作沟覆地膜对旱地马铃薯光合特性及产量形成的影响[J]. 作物学报, 2022, 48(5): 1288-1297.
[3]	王霞, 尹晓雨, 于晓明, 刘晓丹. 干旱锻炼对B73自交后代当代干旱胁迫记忆基因表达及其启动子区DNA甲基化的影响[J]. 作物学报, 2022, 48(5): 1191-1198.
[4]	冯亚, 朱熙, 罗红玉, 李世贵, 张宁, 司怀军. 马铃薯StMAPK4响应低温胁迫的功能解析[J]. 作物学报, 2022, 48(4): 896-907.
[5]	张霞, 于卓, 金兴红, 于肖夏, 李景伟, 李佳奇. 马铃薯SSR引物的开发、特征分析及在彩色马铃薯材料中的扩增研究[J]. 作物学报, 2022, 48(4): 920-929.
[6]	谭雪莲, 郭天文, 胡新元, 张平良, 曾骏, 刘晓伟. 黄土高原旱作区马铃薯连作根际土壤微生物群落变化特征[J]. 作物学报, 2022, 48(3): 682-694.
[7]	丁红, 徐扬, 张冠初, 秦斐斐, 戴良香, 张智猛. 不同生育期干旱与氮肥施用对花生氮素吸收利用的影响[J]. 作物学报, 2022, 48(3): 695-703.
[8]	余慧芳, 张卫娜, 康益晨, 范艳玲, 杨昕宇, 石铭福, 张茹艳, 张俊莲, 秦舒浩. 马铃薯CrRLK1Ls基因家族的鉴定及响应晚疫病菌信号的表达分析[J]. 作物学报, 2022, 48(1): 249-258.
[9]	荐红举, 尚丽娜, 金中辉, 丁艺, 李燕, 王季春, 胡柏耿, Vadim Khassanov, 吕典秋. 马铃薯PIF家族成员鉴定及其对高温胁迫的响应分析[J]. 作物学报, 2022, 48(1): 86-98.
[10]	许德蓉, 孙超, 毕真真, 秦天元, 王一好, 李成举, 范又方, 刘寅笃, 张俊莲, 白江平. 马铃薯StDRO1基因的多态性鉴定及其与根系性状的关联分析[J]. 作物学报, 2022, 48(1): 76-85.
[11]	张明聪, 何松榆, 秦彬, 王孟雪, 金喜军, 任春元, 吴耀坤, 张玉先. 外源褪黑素对干旱胁迫下春大豆品种绥农26形态、光合生理及产量的影响[J]. 作物学报, 2021, 47(9): 1791-1805.
[12]	李洁, 付惠, 姚晓华, 吴昆仑. 不同耐旱性青稞叶片差异蛋白分析[J]. 作物学报, 2021, 47(7): 1248-1258.
[13]	唐锐敏, 贾小云, 朱文娇, 印敬明, 杨清. 马铃薯热激转录因子HsfA3基因的克隆及其耐热性功能分析[J]. 作物学报, 2021, 47(4): 672-683.
[14]	李鹏程, 毕真真, 孙超, 秦天元, 梁文君, 王一好, 许德蓉, 刘玉汇, 张俊莲, 白江平. DNA甲基化参与调控马铃薯响应干旱胁迫的关键基因挖掘[J]. 作物学报, 2021, 47(4): 599-612.
[15]	秦天元, 刘玉汇, 孙超, 毕真真, 李安一, 许德蓉, 王一好, 张俊莲, 白江平. 马铃薯StIgt基因家族的鉴定及其对干旱胁迫的响应分析[J]. 作物学报, 2021, 47(4): 780-786.