水稻内源油菜素甾醇对施氮量的响应及其对颖花退化的调控作用

doi:10.3724/SP.J.1006.2021.02048

作物学报 ›› 2021, Vol. 47 ›› Issue (5): 894-903.doi: 10.3724/SP.J.1006.2021.02048

水稻内源油菜素甾醇对施氮量的响应及其对颖花退化的调控作用

姚佳瑜¹^,²(), 于吉祥¹^,², 王志琴¹^,², 刘立军¹^,², 周娟¹^,², 张伟杨¹^,²^,^*(), 杨建昌¹^,²^,^*()

¹江苏省作物遗传生理重点实验室 / 江苏省作物栽培生理重点实验室 / 扬州大学农学院, 江苏扬州225009
²江苏省粮食作物现代产业技术协同创新中心 / 扬州大学, 江苏扬州225009

收稿日期:2020-07-14 接受日期:2020-11-13 出版日期:2021-05-12 网络出版日期:2020-12-23
通讯作者: 张伟杨,杨建昌
作者简介:E-mail: yaojiayuyzu@163.com
基金资助:
国家自然科学基金项目(31901445);国家自然科学基金项目(31771710);江苏省大学生实践创新训练计划项目(201911117010Z);国家重点研发计划(2016YFD0300206-4);国家重点研发计划(2018YFD0300800);国家重点研发计划(2017YFD0301206);中国博士后科学基金资助项目(2018M640528);江苏高校优势学科建设工程项目(PAPD);扬州大学高端人才支持计划项目(2015-01)

Response of endogenous brassinosteroids to nitrogen rates and its regulatory effect on spikelet degeneration in rice

YAO Jia-Yu¹^,²(), YU Ji-Xiang¹^,², WANG Zhi-Qin¹^,², LIU Li-Jun¹^,², ZHOU Juan¹^,², ZHANG Wei-Yang¹^,²^,^*(), YANG Jian-Chang¹^,²^,^*()

¹Jiangsu Key Laboratory of Crop Genetics and Physiology / Jiangsu Key Laboratory of Crop Cultivation and Physiology / Agricultural College of Yangzhou University, Yangzhou 225009, Jiangsu, China
²Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops / Yangzhou University, Yangzhou 225009, Jiangsu, China

Received:2020-07-14 Accepted:2020-11-13 Published:2021-05-12 Published online:2020-12-23
Contact: ZHANG Wei-Yang,YANG Jian-Chang
Supported by:
Natural Science Foundation of China(31901445);Natural Science Foundation of China(31771710);Training Programs of Innovation and Entrepreneurship for Undergraduates of Jiangsu Province(201911117010Z);Development Program of China(2016YFD0300206-4);Development Program of China(2018YFD0300800);Development Program of China(2017YFD0301206);Project funded by China Postdoctoral Science Foundation(2018M640528);Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD);Top Talent Supporting Program of Yangzhou University(2015-01)

摘要/Abstract

摘要：

为探明油菜素甾醇(brassinosteroids, BRs)是否介导氮肥对水稻颖花退化的影响。水稻品种扬稻6号和甬优2640种植于盆钵, 全生育期设置3种施氮水平, 观察了不同氮肥处理下水稻减数分裂期幼穗中氮含量、BRs、过氧化氢(H₂O₂)和总抗氧化能力(total antioxidant capacity, T-AOC)水平及其与颖花退化的关系。结果表明, 颖花退化率的降低与稻穗中增加的24-表油菜素甾酮(24-epicastasterone, 24-epiCS)和28-高油菜素内酯(28-homobrassinolide, 28-homBL)含量密切相关。当稻穗的氮含量为1.25%时, 幼穗中BRs (24-epiCS和28-homBL)含量显著增加, 颖花退化率显著降低。稻穗中T-AOC水平与BRs含量变化趋势相同, 且均与水稻颖花退化率显著负相关, 而H₂O₂含量与BRs含量和T-AOC变化趋势相反。施用外源BRs (24-epiCS或28-homBL)可显著提高稻穗中内源BRs (24-epiCS和28-homBL)含量与T-AOC水平, 并显著降低稻穗中H₂O₂含量和颖花退化率, 施用BRs合成抑制剂则效果相反。表明BRs可以介导氮肥对水稻颖花退化的调控, 在减数分裂期适宜的稻穗含氮量(1.25%)可有效提高幼穗中的BRs含量, 并通过提高抗氧化能力来抑制颖花退化。

关键词: 油菜素甾醇, 氮素, 水稻, 颖花退化

Abstract:

In order to investigate whether and how brassinosteroids (BRs) mediate the effect of nitrogen (N) rates on spikelet degeneration of rice, rice cultivars Yangdao 6 and Yongyong 2640 were grown in pots subjected to three N rates in the whole growth periods. The contents of N, BRs, hydrogen peroxide (H₂O₂) and total antioxidant capacity (T-AOC) in young rice panicles at meiosis stage and their relationship with spikelet degeneration rate were observed. The results showed that the decreased spikelet degeneration rate was closely associated with enhanced 24-epicastasterone (24-epiCS) and 28-homobrassinolide (28-homBL) contents in young panicles. When N content of rice panicle was 1.25%, the BRs (24-epiCS and 28-homBL) content in young panicle increased significantly, and the spikelet degeneration rate decreased. The variation trend of T-AOC level was very consistent with BRs, and T-AOC was significantly negatively correlated with spikelet degeneration rate, whereas the variation trend of H₂O₂ content was opposite to that of T-AOC and BRs contents in the young panicles. Application of exogenous BRs (24-epiCS or 28-homBL) to young panicles could significantly increase the T-AOC level and contents of endogenous 24-epiCS and 28-homBL, but significantly reduce the H₂O₂ content and spikelet degeneration rate, while application of BRs synthesis inhibitor had the opposite effect. In summary, BRs mediated the effects of N application rates on spikelet degeneration, and elevated BRs contents in young panicles could inhibit spikelet degeneration by elevating antioxidant capacity under a proper panicle N content (1.25%) at meiosis stage in rice.

Key words: brassinosteroids, nitrogen, rice (Oryza sativa L.), spikelet degeneration

姚佳瑜, 于吉祥, 王志琴, 刘立军, 周娟, 张伟杨, 杨建昌. 水稻内源油菜素甾醇对施氮量的响应及其对颖花退化的调控作用[J]. 作物学报, 2021, 47(5): 894-903.

YAO Jia-Yu, YU Ji-Xiang, WANG Zhi-Qin, LIU Li-Jun, ZHOU Juan, ZHANG Wei-Yang, YANG Jian-Chang. Response of endogenous brassinosteroids to nitrogen rates and its regulatory effect on spikelet degeneration in rice[J]. Acta Agronomica Sinica, 2021, 47(5): 894-903.

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年份/品种/处理 Year/cultivar/treatment	每盆穗数 Number of panicle	每穗分化颖花数 Differentiated spikelets per panicle	颖花退化率 Spikelet degeneration rate (%)	每穗粒数 Spikelets per panicle	结实率 Fully-filled grains rate (%)	千粒重 1000-grain weight (g)	产量 Grain yield (g pot^-1)
2015
扬稻6号YD-6
低氮LN	15.0 ± 0.56 c	188 ± 2.69 c	8.32 ± 0.29 b	170 ± 2.95 b	89.1 ± 0.98 a	28.3 ± 0.48 a	62.9 ± 2.20 c
中氮MN	19.9 ± 0.72 b	201 ± 2.87 a	7.49 ± 0.04 c	181 ± 4.22 a	86.5 ± 1.03 b	27.0 ± 0.53 ab	83.7 ± 2.55 a
高氮HN	22.3 ± 0.91 a	195 ± 2.78 b	9.68 ± 0.12 a	172 ± 2.21 b	80.0 ± 0.71 c	25.9 ± 0.83 b	78.2 ± 2.91 b
甬优2640 YY-2640
低氮LN	14.2 ± 0.57 b	293 ± 5.79 c	14.9 ± 0.16 a	245 ± 5.06 b	84.4 ± 1.80 a	25.4 ± 0.37 a	73.6 ± 3.47 b
中氮MN	17.8 ± 0.23 a	336 ±4.80 b	13.4 ± 0.34 b	276 ± 3.73 a	78.7 ± 2.66 b	24.8 ± 0.38 a	95.3 ± 1.61 a
高氮HN	18.5 ± 0.40 a	347 ± 4.96 a	11.8 ± 0.27 c	282 ± 5.76 a	72.7 ± 2.07 c	24.5 ± 0.27 a	91.6 ± 3.41 a
2016
扬稻6号YD-6
低氮LN	14.4 ± 0.36 c	185 ± 2.45 b	8.52 ± 0.56 ab	172 ± 2.25 b	90.3 ± 1.59 a	28.6 ± 0.70 a	65.5 ± 2.38 c
中氮MN	19.5 ± 0.83 b	198 ± 3.19 a	7.55 ± 0.31 b	179 ± 3.43 a	87.1 ± 0.99 b	27.3 ± 0.75 ab	85.0 ± 2.70 a
高氮HN	22.1 ± 0.76 a	191 ± 3.01 ab	9.36 ± 0.47 a	174 ± 2.06 ab	79.0 ± 1.72 c	26.1 ± 1.14 b	80.2 ± 4.42 b
甬优2640 YY-2640
低氮LN	13.8 ± 0.75 c	292 ± 5.76 b	15.8 ± 0.33 a	241 ± 7.12 b	84.1 ± 2.33 a	25.6 ± 0.52 a	73.1 ± 4.05 b
中氮MN	17.4 ± 0.41 b	330 ± 6.23 a	13.8 ± 0.32 b	272 ± 5.69 a	80.7 ± 1.22 b	25.0 ± 0.60 ab	97.3 ± 3.01 a
高氮HN	18.9 ± 0.61 a	341 ± 4.59 a	12.5 ± 0.29 c	278 ± 8.58 a	74.3 ± 1.53 c	24.1 ± 0.49 b	93.6 ± 4.58 a

品种 Cultivar	生理指标 Physiological parameter	化学物质处理 Chemical treatment
品种 Cultivar	生理指标 Physiological parameter	CK	T1	T2	T3	T4
扬稻6号	24-epiCS (pmol g^-1 DW)	37.9 ± 2.28 c	58.6 ± 2.61 a	50.4 ± 1.72 b	14.8 ± 1.01 d	37.2 ± 2.26 c
YD-6	28-homoBL (pmol g^-1DW)	73.7 ± 3.54 c	106 ± 4.99 b	126 ± 3.62 a	35.4 ± 2.07 d	79.3 ± 4.12 c
	T-AOC (U g^-1 DW)	80.3 ± 2.58 b	114 ± 4.09 a	119 ± 5.08 a	34.6 ± 2.46 c	79.8 ± 2.26 b
	H₂O₂ (μmol g^-1 DW)	7.68 ± 0.78 b	4.24 ± 0.26 c	4.17 ± 0.25 c	17.76 ± 0.56 a	7.76 ± 0.68 b
甬优2640	24-epiCS (pmol g^-1DW)	21.5 ± 1.18 c	38.5 ± 1.45 a	31.3 ± 1.21 b	10.8 ± 0.48 d	21.9 ± 0.83 c
YY-2640	28-homoBL (pmol g^-1 DW)	32.2 ± 1.35 c	52.6 ± 2.87 b	62.7 ± 2.07 a	15.3 ± 0.71 d	32.1 ± 1.58 c
	T-AOC (U g^-1 DW)	36.4 ± 1.38 b	50.9 ± 1.74 a	51.7 ± 1.91 a	16.0 ± 0.55 c	35.4 ± 2.23 b
	H₂O₂ (μmol g^-1 DW)	14.9 ± 1.22 b	7.81 ± 0.46 c	7.50 ± 0.49 c	33.8 ± 1.63 a	13.7 ± 0.96 b

品种 Cultivar	处理 Treatment	每穗颖花分化数 Differentiated spikelets per panicle	颖花退化率 Spikelet degeneration rate (%)	每穗粒数 Spikelets per panicle	饱粒率 Fully-filled grains rate (%)	千粒重 1000-grain weight (g)
扬稻6号	CK	204 ± 2.25 a	7.35 ± 0.19 c	185 ± 3.77 bc	88.2 ± 1.69 b	27.2 ± 0.38 a
YD-6	T1	202 ± 3.18 a	4.12 ± 0.17 e	194 ± 3.50 ab	92.3 ± 1.81 a	26.8 ± 0.40 a
	T2	205 ± 2.68 a	4.75 ± 0.09 d	196 ± 3.83 a	92.2 ± 1.80 a	26.6 ± 0.52 a
	T3	201 ± 3.11 a	15.3 ± 0.29 a	172 ± 1.84 c	76.5 ± 1.65 c	27.4 ± 0.84 a
	T4	207 ± 3.49 a	8.03 ± 0.15 b	187 ± 2.61 d	88.3 ± 1.35 b	27.1 ± 0.41 a
甬优2640	CK	346 ± 5.61 a	14.2 ± 0.37 b	292 ± 3.94 b	81.3 ± 1.02 b	24.9 ± 0.40 ab
YY-2640	T1	348 ± 6.63 a	8.92 ± 0.25 c	313 ± 5.40 a	84.2 ± 1.37 a	24.4 ± 0.29 ab
	T2	343 ± 4.95 a	9.07 ± 0.17 c	310 ± 4.56 a	84.4 ± 1.45 a	24.4 ± 0.30 ab
	T3	349 ± 4.42 a	28.4 ± 0.87 a	241 ± 2.97 c	65.3 ± 1.04 c	25.3 ± 0.60 a
	T4	346 ± 5.03 a	14.6 ± 0.51 b	288 ± 4.41 b	80.6 ± 1.53 b	25.0 ± 0.37 ab

水稻内源油菜素甾醇对施氮量的响应及其对颖花退化的调控作用

Response of endogenous brassinosteroids to nitrogen rates and its regulatory effect on spikelet degeneration in rice

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