欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2021, Vol. 47 ›› Issue (5): 974-982.doi: 10.3724/SP.J.1006.2021.01066

• 研究简报 • 上一篇    下一篇

小麦矮秆突变体的鉴定及其突变性状的关联分析

贺军与(), 尹顺琼, 陈云琼, 熊静蕾, 王卫斌, 周鸿斌, 陈梅, 王梦玥, 陈升位*()   

  1. 云南农业大学农学与生物技术学院, 云南昆明 650201
  • 收稿日期:2020-08-16 接受日期:2020-11-13 出版日期:2021-05-12 网络出版日期:2020-12-15
  • 通讯作者: 陈升位
  • 作者简介:E-mail: etfiee_coisini@126.com
  • 基金资助:
    国家自然科学基金项目(31660434);国家自然科学基金项目(32060457)

Identification of wheat dwarf mutants and analysis on association between the mutant traits of the dwarf plants

HE Jun-Yu(), YIN Shun-Qiong, CHEN Yun-Qiong, XIONG Jing-Lei, WANG Wei-Bin, ZHOU Hong-Bin, CHEN Mei, WANG Meng-Yue, CHEN Sheng-Wei*()   

  1. Collage of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, Yunnan, China
  • Received:2020-08-16 Accepted:2020-11-13 Published:2021-05-12 Published online:2020-12-15
  • Contact: CHEN Sheng-Wei
  • Supported by:
    National Natural Science Foundation of China(31660434);National Natural Science Foundation of China(32060457)

RichHTML

22

PDF

831

摘要：

矮秆突变体是小麦育种和株高遗传研究的重要基因资源。通过‘云麦53’成熟种子的EMS (Ethyl methyl sulfonate)诱变及诱变植株连续自交, 获得了33个M3代候选突变体。通过诱变亲本与M2和M3代候选植株的株高差异分析, 筛选到26个矮秆突变体, 其株高变幅为(13.61±0.11)~(44.08±1.73) cm。基于8个矮秆基因的12个特异性标记检测发现, 26个矮秆突变体至少携带2个矮秆基因标记位点。除株高外, 26个矮秆突变体还携带穗长、小穗密度、节间数和平均节间长4个不同突变性状。26个矮秆突变体可聚为5个亚类, 第1亚类的小穗数和小花数最少; 第2亚类的株高最矮, 穗长和平均节间长最短, 小穗密度最高; 第3亚类突变体的节间数最少。株高与平均节间长和节间数呈极显著相关, 偏相关系数分别为0.94、0.58, 但与穗长、小穗数、小花数和小穗密度4个性状无相关性。26个矮秆突变体的株高与平均节间长和节间数关联遗传, 携带不同的突变基因组合, 可用于小麦矮化育种, 以及株高、穗长和小穗密度等性状的遗传机制研究。

关键词: 小麦, 突变体, 矮秆基因, 分子标记, 遗传关联

Abstract:

Dwarf mutant is an important gene resource in wheat breeding and plant height genetic research. In this paper, EMS (ethyl methyl sulfonate) was used to mutate the mature seeds of ‘Yunmai 53’, and 33 candidate dwarf mutants of M3 generation were finally obtained by self-bred. Twenty-six dwarf mutants were selected by analyzing differences between the mutant parent in two years and that of M2 and M3 generation candidate plants, and the variation range of their plant height was from (13.61 ± 0.11) cm to (44.08 ± 1.73) cm. Twenty-six dwarf mutants with at least 2 mutant sites were verified based on 12 specific markers of 8 dwarf genes. In addition to plant height, 26 dwarf mutants also carried four mutational traits, spike length, spikelet density, internode number, and average internode length. The 26 dwarf mutants could be clustered into 5 subgroups. Among them, the first subgroup was the least in spikelets and florets, the second subgroup was the shortest in plant height, spike length and average internode length, and the highest spikelet density, while the third subgroup was the least in internode number. Plant height was significantly correlated with average internode length and internode number with partial correlation coefficients of 0.94 and 0.58, respectively, but not correlated with spike length, spikelet number and spikelet density. The plant height was genetically associated with internode length and internode number in 26 dwarf mutants. The mutants carried different combinations of the mutant gene, and can be useful in wheat dwarf breeding and the studies on genetic mechanism of the traits, such as plant height, spike length and spikelet density.

Key words: wheat, mutant, dwarf genes, molecular marker, genetic association

表1

矮秆基因及其特异性标记的引物序列和片段长度"

基因
Gene		 标记
Marker		 正向引物
Forward sequence
(5′-3′)		 反向引物
Reverse sequence
(5′-3′)		 片段长度
Product size
(bp)
Rht-B1a BF-WR1 GGTAGGGAGGCGAGAGGCGAG CATCCCCATGGCCATCTCGAGCTG 237
Rht-B1b BF-MR1 GGTAGGGAGGCGAGAGGCGAG CATCCCCATGGCCATCTCGAGCTA 237
Rht-D1a BF2-WR2 GGCAAGCAAAAGCTTCGCG GGCCATCTCGAGCTGCAC 264
Rht-D1b BF-MR2 CGCGCAATTATTGGCCAGAGATAG CCCCATGGCCATCTCGAGCTGCTA 254
Rht4 WMC317 TGCTAGCAATGCTCCGGGTAAC TCACGAAACCTTTTCCTCCTCC 170
Rht5 BARC102 GGAGAGGACCTGCTAAAATCGAAGACA GCGTTTACGGATCAGTGTTGGAGA 200
Rht8 Xgwm261 CTCCCTGTACGCCTAAGGC CTCGCGCTACTAGCCATTG 192
Rht8 WMC503 GCAATAGTTCCCGCAAGAAAAG ATCAACTACCTCCAGATCCCGT 225
Rht9 BARC151 TGAGGAAAATGTCTCTATAGCATCC CGCATAAACACCTTCGCTCTTCCACTC 220
Rht12 WMC410 GGACTTGAAAGGAAGCTTGTGA CATGGATGGCATGCAGTGT 114
Rht13 WMS577 ATGGCATAATTTGGTGAAATTG TGTTTCAAGCCCAACTTCTATT 130
Rht14/16/18 XBARC3 TTCCCTGTGTCTTTCTAATTTTTTTT GCGAACTCCCGAACATTTTTAT 210

图1

‘云麦53’与部分矮秆突变体的株高差异 A: 突变体31; B: 突变体17; C: 突变体10; D: 突变体16; E: 突变体23; F: 突变体25。"

表2

‘云麦53’及候选突变体的7个农艺性状均值及其显著性(t测验)"

材料
Material		 株高
Plant height
(cm)		 穗长
Spike length (cm)		 每穗小穗数
Spikelets number
per spike		 小穗密度
Spikelet
density		 小花数
Number of
florets		 平均节间长
Average internode length (cm)		 节间数
Number of
internodes
云麦53 Yunmai 53 74.89±4.03 10.30±0.31 19.25±1.21 1.88±0.18 59.35±5.23 11.10±1.04 6.00±0.00
mutant 1 56.95±6.95 10.49±1.52 20.50±3.50 2.05±0.63 60.34±9.34 7.50±3.33 5.00±0.00
mutant 2 39.52±2.02* 8.40±1.10 17.30±3.30 2.15±0.68 58.20±16.20 7.98±0.78 4.50±0.50
mutant 3 63.99±2.51 6.11±0.59* 17.10±1.10 2.84±0.46 51.70±3.70 11.23±0.96 5.40±0.60
mutant 4 37.24±0.44* 4.44±0.34** 19.30±1.30 4.35±0.04** 55.00±1.00 7.21±0.61 4.50±0.50
mutant 5 21.94±3.66* 3.72±0.02** 20.00±1.00 5.37±0.24** 58.30±1.30 4.88±0.62* 3.80±0.20**
mutant 6 39.85±4.39* 6.01±0.45* 19.10±2.10 3.73±1.27 55.50±4.50 6.87±0.44 4.50±0.50
mutant 7 33.89±2.89* 4.71±0.41** 21.00±2.00 4.46±0.04** 63.40±6.40 7.22±0.39 4.00±0.00
mutant 8 34.92±2.58* 4.40±0.10** 22.50±0.50 5.12±0.01** 67.50±1.50 7.16±0.44 4.40±0.60
mutant 9 31.81±6.79* 4.18±0.58* 21.30±2.30 5.12±0.16** 61.20±4.20 6.20±0.95 4.40±0.60
mutant 10 25.55±7.85* 4.24±0.35** 19.40±1.40 4.62±0.71 54.70±0.70 5.44±1.77 3.90±0.10**
mutant 11 29.78±1.92* 8.20±0.70 16.10±0.90 1.97±0.06 48.00±3.00 5.49±0.24* 4.50±0.50
mutant 12 38.79±2.61* 4.63±0.13** 19.30±2.30 4.16±0.38* 54.40±3.40 7.30±0.08 5.00±0.00
mutant 13 38.32±8.48 3.98±0.38** 15.90±1.90 3.98±0.10** 44.90±2.90 6.65±0.85 4.50±0.50
mutant 14 33.18±6.02* 4.36±0.24** 18.80±0.80 4.34±0.43* 53.00±1.00 5.95±0.03* 3.90±0.01**
mutant 15 30.94±2.26* 4.00±0.50** 17.90±0.10 4.55±0.59* 49.50±4.50 5.68±0.24* 4.80±0.20*
mutant 16 13.61±0.11** 3.39±0.11** 18.40±1.40 5.45±0.59* 45.70±5.30 2.26±0.63* 4.80±1.20
mutant 17 32.62±4.98* 3.96±0.26** 17.50±0.50 4.43±0.16** 49.10±1.90 6.16±0.62 4.50±0.50
mutant 18 44.08±1.73* 7.08±0.48* 13.50±2.50 1.90±0.23 33.25±0.25* 7.87±0.97 5.50±0.50
mutant 19 42.41±12.19 10.58±1.52 21.40±2.40 2.10±0.53 69.10±12.10 6.10±1.01 5.30±0.70
mutant 20 42.18±6.58 7.97±0.63 15.30±0.30 1.94±0.20 43.10±1.90 7.31±1.89 4.80±0.20*
mutant 21 31.26±4.44* 4.02±0.48** 15.60±1.60 3.99±0.88 40.40±1.60 7.04±0.79 3.80±0.20**
mutant 22 52.28±5.48 8.45±0.95 19.50±0.50 2.34±0.33 56.30±0.70 8.44±0.94 5.50±0.50
材料
Material		 株高
Plant height
(cm)		 穗长
Spike length (cm)		 每穗小穗数
Spikelets number
per spike		 小穗密度
Spikelet
density		 小花数
Number of
florets		 平均节间长
Average internode length (cm)		 节间数
Number of
internodes
mutant 23 15.80±0.60** 3.70±0.35** 19.80±1.80 5.28±0.01* 58.80±4.80 2.29±0.41* 4.40±0.60
mutant 24 30.07±4.43* 6.76±1.04 16.90±2.90 2.63±0.84 49.70±7.70 5.26±0.05* 5.00±0.00
mutant 25 18.16±4.44* 3.77±0.17** 20.20±0.20 5.37±0.19** 58.80±1.20 3.93±0.93* 4.50±0.50
mutant 26 19.75±1.35** 4.60±1.00* 18.60±1.60 4.17±0.56 53.20±2.20 4.16±0.46* 3.80±0.20**
mutant 27 34.54±4.74* 7.49±0.89 14.80±3.80 1.95±0.28 50.00±17.00 7.13±1.40 4.50±0.50
mutant 28 33.76±10.16 8.86±0.16 15.90±1.90 1.79±0.18 53.90±11.90 6.59±3.49 5.00±0.00
mutant 29 35.01±4.79* 8.20±0.40 16.40±0.40 2.01±0.15 49.30±1.30 6.18±0.02* 5.00±0.00
mutant 30 30.09±6.01* 8.57±1.13 16.10±1.10 1.93±0.38 48.60±3.60 5.92±0.00* 4.30±0.70
mutant 31 33.96±0.36* 8.70±1.00 17.20±0.20 2.01±0.26 49.20±1.80 5.60±0.94 4.50±0.50
mutant 32 31.50±0.70** 8.02±0.28* 16.50±1.50 2.07±0.26 46.30±1.30 5.11±0.53* 4.50±0.50
mutant 33 24.02±3.38* 10.04±1.94 16.63±1.63 1.69±0.17 47.75±2.75 3.95±0.12* 5.00±1.00

图2

部分矮秆突变体及其诱变亲本的穗部形态特征 (a): ‘云麦53’; (b), (c), (d)和(e): 候选矮秆突变体。"

表3

‘云麦53’和候选突变体中检测到的矮秆基因"

材料
Material		 检测到的基因
Genes detected by 13 markers
云麦53 Yunmai 53 Rht-B1a, Rht-D1b, Rht4, Rht8, Rht9, Rht12, Rht14, Rht16, Rht18
mutant 1 Rht-B1a, Rht-D1a, Rht4, Rht5, Rht8#, Rht9#, Rht13, Rht14, Rht16, Rht18
mutant 2 Rht-B1a, Rht-D1b, Rht4, Rht5, Rht8#, Rht9#, Rht13, Rht14, Rht16, Rht18
mutant 3 Rht-B1a, Rht-D1b, Rht4, Rht8#, Rht9#, Rht12, Rht14, Rht16, Rht18
mutant 10 Rht-B1a, Rht-D1a, Rht4, Rht8, Rht9#
mutant 11 Rht-B1a, Rht-D1a, Rht5, Rht12
mutant 19 Rht-B1a, Rht-D1a, Rht4, Rht5, Rht8, Rht9#, Rht14, Rht16, Rht18
mutant 26 Rht-B1a, Rht-D1b, Rht4, Rht8, Rht9, Rht13
mutant 27 & mutant 28 Rht-B1a, Rht-D1b, Rht4, Rht8#, Rht9#, Rht13
Other mutants Rht-B1a, Rht-D1a, Rht4, Rht5, Rht8, Rht9#

图3

‘云麦53’和候选突变体的部分特异性扩增标记 CK: 对照; M: marker; 53: 云麦53; 1~33: 突变体1~突变体33; A和B分别是‘云麦53’和候选突变体中扩增的WMC503和BARC151标记; 红色箭: 差异片段。"

图4

基于7个农艺性状的26个突变体和诱变亲本聚类结果"

图5

不同类群材料的农艺性状均值及其差异 I: ‘云麦53’类群; II~VI: 突变体的第1亚类~第5亚类。不同小写字母和大写字母分别代表0.05和0.01显著水平。"

附表1

‘云麦53’类群和5个矮秆突变体亚类的方差分析结果"

性状
Traits		 变异来源
SOV		 平方和
SS		 自由度
df		 均方
MS		 F值
F-value
株高Plant height (cm) 组间Between groups 11,981.952 5 2396.390 37.615**
组内Within group 4077.292 64 63.708
穗长Spike length (cm) 组间Between groups 119.531 5 23.906 4.603**
组内Within group 332.417 64 5.194
小穗数Spikelets number per spike 组间Between groups 234.415 5 46.883 10.229**
组内Within group 293.328 64 4.583
小穗密度Spikelet density 组间Between groups 48.716 5 9.743 6.920**
组内Within group 90.110 64 1.408
小花数Number of florets 组间Between groups 3126.921 5 625.384 11.289**
组内Within group 3545.316 64 55.396
平均节间长Average internode length (cm) 组间Between groups 190.020 5 38.004 13.307**
组内Within group 182.776 64 2.865
节间数Number of Internode 组间Between groups 14.628 5 2.926 7.604**
组内Within group 24.623 64 0.385

表4

不同突变性状的二元相关和偏相关系数及其显著性"

性状
Trait		 株高
Plant height (cm)		 穗长
Spike length (cm)		 小穗数
Spikelets number per spike		 小穗密度
Spikelet
density		 小花数
Number of florets		 平均节间长
Average internode length (cm)		 节间数
Number of internode
株高 Plant height (cm) 0.51** -0.09 -0.47* 0.06 0.92** 0.59**
穗长 Spike length (cm) 0.26 -0.54* -0.95** -0.25 0.36 0.53**
小穗数 Spikelets number per spike 0.26 0.41 0.74** 0.89** -0.07 -0.27
小穗密度 Spikelet density -0.11 -0.95** 0.64** 0.47* -0.38 -0.48**
小花数 Number of florets -0.32 0.13 0.78** -0.09 0.10 -0.23
平均节间长 Average internode length (cm) 0.94** -0.43* -0.16 -0.29 0.35 0.35
节间数 Number of internode 0.58** 0.16 -0.10 0.16 -0.08 -0.44*
[1] 孙树贵, 李艳丽, 鲁敏, 张军, 王秀娟, 武军, 赵继新, 杨群慧, 刘淑会, 陈新宏. 67份美国小麦品种矮秆基因的分子标记检测. 麦类作物学报, 2013,33:1087-1092.
Sun S G, Li Y L, Lu M, Zhang J, Wang X J, Wu J, Zhao J X, Yang Q H, Liu S H, Chen X H. Distribution of dwarfing genes in 67 American wheat cultivars detected by molecular markers. J Triticeae Crops, 2013,33:1087-1092 (in Chinese with English abstract).
[2] 张德强, 宋晓朋, 冯洁, 马文洁, 武炳瑾, 张传量, 崔紫霞, 冯毅, 孙道杰. 黄淮麦区小麦品种矮秆基因Rht-Blb、Rht-Dlb和Rht8的检测及其对农艺性状的影响. 麦类作物学报, 2016,36:975-981.
Zhang D Q, Song X P, Feng J, Ma W J, Wu B J, Zhang C L, Cui Z X, Feng Y, Sun D J. Detection of dwarf genes Rht-B1b, Rht-D1b and Rht8 in Huang-Huai Valley winter wheat areas. J Triticeae Crops, 2016,36:975-981 (in Chinese with English abstract).
[3] 昝凯, 李春游, 敬樊, 陈真真, 王亮明, 王秀娟, 杨群慧, 王中华, 陈新宏. 部分印度小麦品种矮秆基因的检测及其对部分性状的影响. 麦类作物学报, 2015,35:910-917.
Zan K, Li C Y, Jing F, Chen Z Z, Wang L M, Wang X J, Yang Q H, Wang Z H, Chen X H. Detection of dwarfing genes in some India wheat cultivars and their influences on partial agronomic characteristics. J Triticeae Crops, 2015,35:910-917 (in Chinese with English abstract).
[4] 周强, 袁中伟, 欧俊梅, 任勇, 杜小英, 陶军, 李生荣, 刘登才. 四川小麦主要矮秆基因的分子鉴定. 麦类作物学报, 2015,35:1624-1630.
Zhou Q, Yuan Z W, Ou J M, Ren Y, Du X Y, Tao J, Li S R, Liu D C. Molecular identification of the main dwarfing genes in wheat varieties in Sichuan. J Triticeae Crops, 2015,35:1624-1630 (in Chinese with English abstract).
[5] 朱浩, 牛艳路, 王佩, 全军利, 陈耀锋. 小麦矮秆基因的分子检测及遗传效应研究. 分子植物育种, 2018,16:1855-1861.
Zhu H, Niu Y L, Wang P, Quan J L, Chen Y F. Molecular detection and genetic effect research of dwarf gene in wheat. Mol Plant Breed, 2018,16:1855-1861 (in Chinese with English abstract).
[6] 周强, 袁中伟, 张连全, 甯顺腙, 任勇, 陶军, 李生荣, 刘登才. 四倍体小麦地方品种矮蓝麦矮秆性状的遗传分析. 作物学报, 2015,41:1899-1905.
Zhou Q, Yuan Z W, Zhang L Q, Ning S Z, Ren Y, Tao J, Li S R, Liu D C. Genetic Analysis on dwarfing trait in landrace Ailanmai of Triticum turgidum L. ssp. turgidum. Acta Agron Sin, 2015,41:1899-1905 (in Chinese with English abstract).
[7] 钟明志, 魏淑红, 彭正松, 杨在君. 小麦Rht矮秆基因研究和应用综述. 分子植物育种, 2018,16:6670-6677.
Zhong M Z, Wei S H, Peng Z S, Yang Z J. A review of the research and application of Rht dwarf genes in wheat. Mol Plant Breed, 2018,16:6670-6677 (in Chinese with English abstract).
[8] Emma M J, Rachel E J, Alanna J O, John M M, Michael J G. The impact of the wheat Rht-B1b semi-dwarfing allele on photosynthesis and seed development under field conditions. Front Plant Sci, 2019,10:10-51.
[9] 陈亮. 矮秆基因Rht12对小麦重要农艺性状的遗传效应及新矮秆突变体的筛选. 西北农林科技大学博士学位论文, 陕西杨凌, 2014. pp 10-19.
Chen L. Genetic Effects of Dwarfing Gene Rht12 on the Important Agronomic Traits of Common Bread Wheat and Screening of New Dwarf Mutants. PhD Dissertation of Northwest A&F University, Yangling, Shaanxi, China, 2014. pp 10-19 (in Chinese with English abstract).
[10] 唐娜, 逯芳芳, 何蓓如, 胡银岗. 矮秆基因对小麦部分农艺性状的效应. 西北植物学报, 2010,30:41-49.
Tang N, Lu F F, He B R, Hu Y G. Effects of dwarfing genes on some agronomic characteristics of wheat. Acta Bot Boreal- Occident Sin, 2010,30:41-49 (in Chinese with English abstract).
[11] 刘晴, 古佳玉, 赵紫伟, 赵林姝, 郭会君, 谢永盾, 宋希云, 刘录祥. 小麦矮秆突变体DC20的转录组分析. 核农学报, 2019,33:1451-1458.
Liu Q, Gu J Y, Zhao Z W, Zhao L S, Guo H J, Xie Y D, Song X Y, Liu L X. RNA-Seq analysis of wheat dwarf mutant DC20. J Nucl Agric Sci, 2019,33:1451-1458 (in Chinese with English abstract).
[12] 陆燕, 赵天祥, 刘国祥, 贾继增, 孔秀英. 小麦矮秆圆粒突变体的鉴定与分析. 植物遗传资源学报, 2014,15:160-164.
Lu Y, Zhao T X, Liu G X, Jia J Z, Kong X Y. Identification and analysis of the dwarf-spherical grain mutant W98. J Plant Genet Resour, 2014,15:160-164 (in Chinese with English abstract).
[13] 李杏普, 兰素缺, 张业伦, 张京惠, 冯延茹, 刘书娥, Gale M D, Worland T J. Rht8、Rht10、Rht12矮秆基因对小麦营养生长和生殖生长发育的影响. 华北农学报, 2009,24(增刊1):50-53.
Li X P, Lan S Q, Zhang Y L, Zhang J H, Feng Y R, Liu S E, Gale M D, Worland T J. Effects of Rht8, Rht10 and Rht12 semi-dwarfing and dwarfing genes on wheat vegetative and reproductive development. Acta Agric Boreali-Sin, 2009,24(S1):50-53 (in Chinese with English abstract).
[14] 王清海, 杨在君, 魏淑红, 廖明莉, 苏瑾, 杨宇凤, 杨会, 王育伟, 彭正松. 四倍体小麦矮秆基因的赤霉素敏感性及对农艺性状的影响. 华北农学报, 2015,30(2):132-139.
Wang Q H, Yang Z J, Wei S H, Liao M L, Su J, Yang Y F, Yang H, Wang Y W, Peng Z S. Gibberellin sensitivity of the Rht genes in tetraploid wheat and effects on agronomic traits. Acta Agric Boreali-Sin, 2015,30(2):132-139 (in Chinese with English abstract).
[15] Baloch G A, Cui L, Hu Y G. The effects of dwarfing gene Rht-8 on plant height and other agronomic traits in common wheat. Agric Res Arid Areas, 2014,32:245-252.
[16] Sun L H, Yang W L, Li Y F, Shan Q Q, Ye X B, Wang D Z, Yu K, Lu W W, Xin P Y, Pei Z, Guo X L, Liu D C, Sun J Z, Zhan K H, Chu J F, Zhang A M. A wheat dominant dwarfing line with Rht12, which reduces stem cell length and affects gibberellic acid synthesis, is a 5AL terminal deletion line. Plant J, 2019,97:887-900.
[17] 卢媛, 崔超凡, 胡平, 陈佩度, 沈雪芳, 韩晴, 王义发, 邢莉萍, 曹爱忠. 矮秆基因Rht_NM9在小麦株高建成中对内源激素含量的影响. 作物学报, 2017,43:1272-1279.
Lu Y, Cui C F, Hu P, Chen P D, Shen X F, Han Q, Wang Y F, Xing L P, Cao A Z. Effects of dwarf gene Rht_NM9 on contents of endogenous hormone regulating plant height of common wheat. Acta Agron Sin, 2017,43:1272-1279 (in Chinese with English abstract).
[18] Ellis M H, Rebetzke G J, Azanza F, Richards R A, Spielmeyer W. Molecular mapping of gibberellin responsive dwarfing genes in bread wheat. Theor Appl Genet, 2005,111:423-430.
[19] Ellis M H, Spielmeyer W, Rebetzke G J, Richards R A. Perfect markers for the and dwarfing genes in wheat. Theor Appl Genet, 2002,105:1038-1042.
[20] Watanabe N. Genetic collection and development of near-isogenic lines in durum wheat. Czech J Genet Plant, 2008,12:636-643.
[21] Korzun V, Roeder M S, Ganal M W, Worland A J, Law C N. Genetic analysis of the dwarfing gene in wheat: Part I. molecular mapping of on the short arm of chromosome 2D of bread wheat. Theor Appl Genet, 1998,96:1104-1109.
[22] 贾影影. 小麦矮秆基因Rht13与Rht-D1b互作研究及Rht13分子标记开发. 宁夏大学硕士学位论文, 宁夏银川, 2016. pp 22-26.
Jia Y Y. Study on Interaction of Dwarfing Gene Rht13 with Rht-D1b and Development Molecular Markers for Rht13 in Wheat. MS Thesis of Ningxia University, Yinchuan, Ningxia, China, 2016. pp 22-26 (in Chinese with English abstract).
[23] 吴同彦, 谢令琴, 杨学举, 张彩英, 陈荣芬. 小麦株高构成因素与产量及其他性状相关性的研究. 河北农业大学学报, 2002,25(3):10-12.
Wu T Y, Xie L Q, Yang X J, Zhang C Y, Chen R F. Study on the correlations between the components of the plant height and the yield and other traits of wheat. J Agric Univ Hebei, 2002,25(3):10-12(in Chinese with English abstract).
[24] 闵东红, 王辉, 孟超敏, 翟耀锋, 李学军, 曹宁, 于新智. 不同株高小麦品种抗倒伏性与其亚性状及产量相关性研究. 麦类作物学报, 2001,21(4):76-79.
Min D H, Wang H, Meng C M, Zhai Y F, Li X J, Cao N, Yu X Z. Studies on the lodging resistance with its subtraits of different height wheat varieties and correlation between plant height and yield. J Triticeae Crops, 2001,21(4):76-79 (in Chinese with English abstract).
[25] 赵万春, 王红. 小麦株高及其构成因素的遗传和相关性研究. 麦类作物学报, 2003,23(4):28-31.
Zhao W C, Wang H. Genetic and correlation study on plant height and its components in wheat. J Triticeae Crops, 2003,23(4):28-31 (in Chinese with English abstract).
[26] 张坤普, 徐宪斌, 田纪春. 小麦籽粒产量及穗部相关性状的QTL定位. 作物学报, 2009,35:270-278.
Zhang K P, Xu X B, Tian J C. QTL Mapping for grain yield and spike related traits in common wheat. Acta Agron Sin, 2009,35:270-278 (in Chinese with English abstract).
[27] 张晶, 张定一, 王姣爱, 党建友. 小麦单株有效分蘖数与农艺性状的相关性研究. 山西农业科学, 2009,37(6):17-19.
Zhang J, Zhang D Y, Wang J A, Dang J Y. The dependence study of the effective tillers per plant and agronomic characters in wheat. J Shanxi Agric Sci, 2009,37(6):17-19 (in Chinese with English abstract).
[28] 卢翔, 张锦鹏, 王化俊, 杨欣明, 李秀全, 李立会. 小麦-冰草衍生后代3558-2穗部相关性状的遗传分析和QTL定位. 植物遗传资源学报, 2011,12:86-91.
Lu X, Zhang J P, Wang H J, Yang X M, Li X Q, Li L H. Genetic analysis and QTL mapping of wheat spike traits in a derivative line 3558-2 from wheat × Agropyron cristatum offspring. J Plant Genet Resour, 2011,12:86-91 (in Chinese with English abstract).
[29] 张祥池, 郝燕冉, 魏凡, 张志锋, 魏佳佳, 付帅, 安浩军, 王睿辉. 小麦矮秆突变体矮秆基因的遗传分析. 河北农业大学学报, 2017,40(2):5-10.
Zhang X C, Hao Y R, Wei F, Zhang Z F, Wei J J, Fu S, An H J, Wang R H. Characterization of the Rht gene in one dwarfing mutant of wheat ( Triticum aestivum L.). J Agric Univ Hebei, 2017,40(2):5-10 (in Chinese with English abstract).
[30] 冯洁, 许小宛, 李小东, 张传量, 崔紫霞, 冯毅, 孙道杰. 黄淮麦区小麦品种和CIMMYT材料的矮秆基因型及其对株高和胚芽鞘的影响. 麦类作物学报, 2018,38:668-673.
Feng J, Xu X W, Li X D, Zhang C L, Cui Z X, Feng Y, Sun D J. Dwarf genotype of wheat from Huang-Huai river wheat area and CIMMYT and their effects on plant height and coleoptile length. J Triticeae Crops, 2018,38:668-673 (in Chinese with English abstract).
[31] 吴儒刚, 裴艳婷, 张超, 范业泉, 靳义荣, 刘鹏, 贾德新, 戴忠民. 基于盐胁迫的小麦农艺性状多样性分析及评价. 麦类作物学报, 2019,39:1029-1037.
Wu R G, Pei Y T, Zhang C, Fan Y Q, Jin Y R, Liu P, Jia D X, Dai Z M. Analysis and evaluation of agronomic character diversity of wheat based on salt stress. J Triticeae Crops, 2019,39:1029-1037 (in Chinese with English abstract).
[32] 林旭群, 李韵芳, 鲁璐, 张春艳, 廖金花, 李莉蓉, 吴瑜. 小麦矮秆基因Rht-B1b、Rht-D1b、Rht8的检测及其对株高的影响. 应用与环境生物学报, 2014,20:80-86.
Lin X Q, Li Y F, Lu L, Zhang C Y, Liao J H, Li L R, Wu Y. Identification of wheat dwarfing genes Rht-B1b, Rht-D1b, Rht8 and their effects on plant height. Chin J Appl Environ Biol, 2014,20:80-86 (in Chinese with English abstract).
[33] 王鑫, 马莹雪, 杨阳, 王丹峰, 殷慧娟, 王洪刚. 小麦矮秆种质SN224的鉴定及农艺性状QTL分析. 作物学报, 2016,42:1134-1142.
Wang X, Ma Y X, Yang Y, Wang D F, Yin H J, Wang H G. Identification of dwarfing wheat germplasm SN224 and analysis of QTLs for its agronomic characters. Acta Agron Sin, 2016,42:1134-1142 (in Chinese with English abstract).
[34] 杨秋平, 杨阳, 王鑫, 马莹雪, 张明, 王洪刚. 小麦矮秆种质山农11069-5矮秆基因的遗传分析及分子定位. 分子植物育种, 2015,13:71-76.
Yang Q P, Yang Y, Wang X, Ma Y X, Zhang M, Wang H G. Genetic analysis and molecular mapping of the gene in dwarf germplasm Shannong 11069-5. Mol Plant Breed, 2015,13:71-76 (in Chinese with English abstract).
[35] 张明, 吴瑕, 张一铎, 张超, 牛祖彪, 崔淑佳, 杨秋平, 王洪刚. 小麦矮秆种质系山农342-9矮秆基因的分子标记定位. 山东农业科学, 2014,46(7):7-10.
Zhang M, Wu X, Zhang Y D, Zhang C, Niu Z B, Cui S J, Yang Q P, Wang H G. Molecular mapping of Rht Gene in wheat dwarfing germplasm line Shannong 342-9. Shandong Agric Sci, 2014,46(7):7-10 (in Chinese with English abstract).
[1] 翟胜男, 曹新有, 李豪圣, 李吉虎, 李法计, 刘金栋, 夏先春, 吕莹莹, 马瑞峰, 王颖, 耿洪伟, 刘建军. 小麦Pod-A1、Pod-D1和Pod-2D位点等位变异对籽粒过氧化物酶活性的遗传效应分析[J]. 作物学报, 2026, 52(6): 1593-1603.
[2] 习千辉, 徐梓瑗, 刘梦梦, 王宏艺, 郎凯琳, 井震海, 陈锋, 赵磊. 小麦籽粒铜含量的全基因组关联分析及候选基因预测[J]. 作物学报, 2026, 52(6): 1604-1617.
[3] 毛嘉琦, 黄朋雨, 赵佳佳, 郑兴卫, 武棒棒, 郝宇琼, 屈非, 刘成, 马朋涛, 郑军. 山西小麦品种白粉病抗性评价及抗病基因分子检测[J]. 作物学报, 2026, 52(6): 1669-1681.
[4] 胡川, 赵凯男, 黄修利, 吴金芝, 任开明, 王贺正, 付国占, 黄明, 李友军. 一次灌溉下耕作方式和氮肥用量对旱地小麦产量和品质的影响[J]. 作物学报, 2026, 52(6): 1830-1846.
[5] 陈雪燕, 何华川, 李政嘉, 董新盼, 李藕琪, 刘小云, 李丹萍, 陈志伟, 刘国霞, 吕胜源, 吴印莹, 赵振东, 曹新有, 万何平. 水培盐碱复合胁迫下‘济麦60’苗期根系有机酸分泌动态变化及其转录调控机制[J]. 作物学报, 2026, 52(6): 1859-1875.
[6] 彭佳泺, 李颖, 李丹丹, 杨军宁, 郭学峰, 张文姣, 俞晓雪, 周亚荣, 王振玉, 王彩香, 马雄风, 宿俊吉. 陆地棉I类LBD家族成员鉴定及GhLBD6调控开花期的功能和单倍型分析[J]. 作物学报, 2026, 52(6): 1682-1697.
[7] 高沛阳, 李瑾璇, 董宇奎, 石玉, 张振, 张永丽. 测墒补灌下小麦分蘖发生和成穗对施氮量的响应[J]. 作物学报, 2026, 52(6): 1847-1858.
[8] 张献丰, 郭利建, 李康春, 孔斌雪, 刘玉芳, 车卓, 杨德龙. 小麦ABHD6基因家族鉴定与粒重功能标记开发[J]. 作物学报, 2026, 52(6): 1711-1727.
[9] 张颖星, 宋裕祯, 王跃, 曹越, 曹晓宁, 王瑞云. EMS诱导糜子优异性状突变体的筛选及表型分析[J]. 作物学报, 2026, 52(5): 1388-1400.
[10] 王壮壮, 武紫君, 张永新, 张芯源, 袁丽雪, 陈如雪, 刘世举, 段剑钊, 冯伟, 王同朝, 王永华. 豫东南黏壤潮土区水氮优化协同提高冬小麦产量和氮素利用效率[J]. 作物学报, 2026, 52(5): 1501-1521.
[11] 何万龙, 耿洪伟, 张飞飞, 米克热阿依·阿巴白克热, 罗紫洋, 李鹏程, 周钊宇, 程宇坤. 基于深度学习的小麦重要病害图像识别系统的研究[J]. 作物学报, 2026, 52(5): 1401-1417.
[12] 张振, 冯连杰, 石玉, 于振文, 张永丽. 节水补灌下不同穗型小麦产量形成差异研究[J]. 作物学报, 2026, 52(5): 1522-1535.
[13] 侯思宇, 王国璀, 韦金贵, 谢玮欣, 殷文, 樊志龙, 柴强, 胡发龙. 绿肥配施化学氮肥对西北干旱灌区小麦干物质积累及产量形成的影响[J]. 作物学报, 2026, 52(4): 1208-1219.
[14] 尚云秋, 赵竹, 陈欢, 丁永刚, 乔玉强, 李玮, 张向前, 曹承富, 杜世州. 长期定位耕作方式对雨养小麦籽粒灌浆和产量形成的影响[J]. 作物学报, 2026, 52(4): 1236-1250.
[15] 乔宇馨, 李程越, 康晓玉, 张鑫琪, 贾绍辉, 刘倩, 曹亚丽, 史鑫蕊, 郝兴宇, 李萍. 基于APSIM模型的长期免耕秸秆覆盖对旱地小麦增产效应研究[J]. 作物学报, 2026, 52(4): 1181-1192.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2