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作物学报 ›› 2021, Vol. 47 ›› Issue (8): 1472-1480.doi: 10.3724/SP.J.1006.2021.02056

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用单片段代换系鉴定巴西陆稻IAPAR9中的粒型基因

张波(), 裴瑞琴, 杨维丰, 朱海涛, 刘桂富, 张桂权, 王少奎*()   

  1. 广东省植物分子育种重点实验室/亚热带农业生物资源保护与利用国家重点实验室/华南农业大学, 广东广州 510642
  • 收稿日期:2020-08-17 接受日期:2021-01-13 出版日期:2021-08-12 网络出版日期:2021-02-25
  • 通讯作者: 王少奎
  • 作者简介:E-mail: zhangboscau@163.com
  • 基金资助:
    国家重点研发计划项目(2016YFD0100406);广东省基础与应用基础研究重大项目(2019B030302006);广东省特支计划项目(2016TX03N224)

Mapping and identification QTLs controlling grain size in rice (Oryza sativa L.) by using single segment substitution lines derived from IAPAR9

ZHANG Bo(), PEI Rui-Qing, YANG Wei-Feng, ZHU Hai-Tao, LIU Gui-Fu, ZHANG Gui-Quan, WANG Shao-Kui*()   

  1. Guangdong Provincial Key Laboratory of Plant Molecular Breeding/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/South China Agricultural University, Guangzhou 510642, Guangdong, China
  • Received:2020-08-17 Accepted:2021-01-13 Published:2021-08-12 Published online:2021-02-25
  • Contact: WANG Shao-Kui
  • Supported by:
    National Key Research and Development Program of China(2016YFD0100406);Key Projects of Basic Research and Applied Basic Research of Guangdong Province(2019B030302006);Special Support Program of Guangdong Province for High-level Talents(2016TX03N224)

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摘要：

水稻粒型是由多基因控制的复杂数量性状, 是稻米产量和品质的重要影响因子, 水稻粒型基因的定位与遗传研究有助于稻米产量与品质的协同改良。本研究利用巴西陆稻IAPAR9为供体、以华南地区高产籼稻品种华粳籼74为受体, 构建的153份水稻单片段代换系材料, 连续两季通过单因素方差分析和邓肯氏多重比较, 结合代换片段重叠群作图, 定位了13个控制水稻粒型及粒重的QTL。这13个位点分别分布于水稻1、2、4、5、6、7、9和11号染色体上, 包括9个控制谷粒长的QTL、1个控制谷粒宽的QTL和3个控制千粒重的QTL。其中, qGL1-2、qTGW1-2和qGL11为新鉴定的QTL位点。新的粒型QTL定位将为进一步的基因克隆与粒型遗传调控网络解析提供依据和线索, 也可为稻米产量与品质协同改良提供新的种质资源。

关键词: 水稻, 单片段代换系, 粒型, QTL定位

Abstract:

Rice grain size is a complex quantitative trait controlled by multiple genes. Grain size is an important factor affecting rice yield and quality. The mapping and genetic analysis of genes controlling rice grain size are essential for the concurrent improvement of rice yield and quality. Here 13 QTLs for grain size were detected using 153 rice single-segment substitution lines in rice, which were derived from HJX74 as the receptor parent, and IAPAR9 as the donor parent. One-way ANOVA and Duncan’s multiple comparison were employed to detect the genetic bases of rice grain size in two consecutive years. Based on the substitution mapping using overlapped substitution-fragment in the SSSLs, a total of 13 grain size-related QTLs were detected on chromosomes 1, 2, 4, 5, 6, 7, 9, and 11, including nine QTLs controlling grain length, one QTL controlling grain width, and three QTLs controlling 1000-grain weight. Furthermore, qGL1-2, qTGW1-2, and qGL11 were novel identified QTLs. This study provided new basis for cloning and functional analysis of genes regulating grain size.

Key words: rice, single segment substitution line, grain size, QTL mapping

图1

IAPAR9与HJX74的粒型 a, b: HJX74和IAPAR9的粒型, 标尺为5 mm; c: HJX74和IAPAR9的粒长; d: HJX74和IAPAR9的粒宽; e: HJX74和IAPAR9的粒重。**表示HJX74的粒长、粒宽和千粒重与IAPAR9之间的差异达到极显著水平(P < 0.01), t检验。"

附表1

引物名称及序列"

标记名称
Marker		 基因
Gene		 序列
Sequence (5′-3′)		 标记名称
Marker		 基因
Gene		 序列
Sequence (5′-3′)
xp14F LGY3 TGTCAACAGAAACCAGCAAAA RMw513F GW5 GTATTTGTTTGTCGCATTC
xp14R TGGTCGATCAATTCCTCTGC RMw513R TAGGACCATAGATGTGAGTTA
GS3R1 gs3 AGGCTGGCTTACTCGCTG RM574F GS5 GGCGAATTCTTTGCACTTGG
GS3R2 CAGCAGGCTGGCTTACTCTATT RM574R ACGGTTTGGTAGGGTGTCAC
GS3F CTGTATATATATTTCTTGCAGGGTG Indel-1F GW7 CCATAGTAAGACGACCTT
RM186F GL3.1 TCCTCCATCTCCTCCGCTCCCG Indel-1R GATATTCTGTCAGCAGTT
RM186R GGGCGTGGTGGCCTTCTTCGTC PSM710F gw8 GCCAGCCAAGAAAAGCGACA
PSM710R TCTTGAGATCCCACTCCATG

图2

HJX74与IAPAR9的粒型基因分子标记多态性 白色线段指示相关引物经PCR扩增获得的目的条带, 其余带纹为引物非特异性扩增条带。"

图3

代换片段在水稻染色体上的覆盖率 遗传距离(单位: cM)见左侧标尺; 染色体上黑色部分代表该区域可以被来自IAPAR9的代换片段所覆盖; 蓝色字体突出显示相关位点上已克隆的水稻粒型基因。"

附表2

单片段代换系代换片段分布"

染色体
Chr.		 单片段材料数量
No. of SSSLs		 代换片段总长度
TL (cM)		 代换片段平均长度
CL (cM)		 代换片段覆盖率
CP (%)
Chr. 1 30 571.00 19.03 59.16
Chr. 2 22 526.00 23.91 78.31
Chr. 3 4 112.40 28.10 35.21
Chr. 4 14 380.70 27.19 43.90
Chr. 5 12 179.30 14.94 53.98
Chr. 6 24 541.40 22.56 73.55
Chr. 7 7 75.20 10.74 19.27
Chr. 8 8 181.40 22.68 66.91
Chr. 9 9 256.70 28.52 85.03
Chr. 10 6 160.60 26.77 55.93
Chr. 11 16 320.60 20.04 60.04
Chr. 12 1 6.90 6.90 3.15
平均Mean 12.75 276.02 20.95 52.87

图4

亲本和SSSL材料粒长、粒宽和千粒重频次分布 a~c: 2018晚季亲本和SSSL的粒长、粒宽和千粒重的频次分布; d~f: 2019早季亲本和SSSL的粒长、粒宽和千粒重的频次分布。"

图5

13个粒型及千粒重性状QTL在水稻染色体上的分布 遗传距离见左侧标尺; 染色体右侧的黑色矩形代表QTL及其定位区间。"

表1

利用SSSL定位到的粒型QTL及其加性效应"

性状
Trait		 位点
QTL		 染色体
Chr.		 区间
Marker interval		 加性效应Additive effect P值P-value
2018 2019 2018 2019
粒长Grain length qGL1-1 1 PSM13-RM84 -0.19 -0.11 0.0025 0.0188
qGL1-2 1 OSR23-RM104 0.30 0.31 0.0001 0.0005
qGL2 2 RM250-RM166 -0.19 -0.15 <0.0001 0.0029
qGL4 4 RM317-PSM110 -0.23 -0.15 0.0002 0.0081
qGL5 5 RM164-RM161 -0.19 -0.12 0.0002 0.0289
qGL6 6 PSM136-RM275 -0.22 -0.15 <0.0001 0.0227
qGL7 7 RM505-PSM432 0.37 0.43 0.0003 <0.0001
qGL9 9 PSM399-RM105 -0.20 -0.15 <0.0001 0.0084
qGL11 11 RM209-RM21 -0.17 -0.16 0.0005 0.0023
粒宽Grain width qGW2 2 RM263-RM525 0.09 0.08 0.0003 0.0039
千粒重1000-grain weight qTGW1-1 1 PSM13-RM84 -1.46 -1.32 <0.0001 0.0016
qTGW1-2 1 OSR23-RM104 0.65 1.25 0.0003 0.0011
qTGW4 4 RM127-RM280 -1.31 -1.45 <0.0001 <0.0001
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