欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2021, Vol. 47 ›› Issue (9): 1791-1805.doi: 10.3724/SP.J.1006.2021.04154

• 研究论文 • 上一篇    下一篇

外源褪黑素对干旱胁迫下春大豆品种绥农26形态、光合生理及产量的影响

张明聪1(), 何松榆1(), 秦彬1, 王孟雪1, 金喜军1, 任春元1, 吴耀坤2, 张玉先1,*   

  1. 1黑龙江八一农垦大学农学院, 黑龙江大庆 163319
    2黑龙江省农业科学院大庆分院, 黑龙江大庆 163316
  • 收稿日期:2020-07-11 接受日期:2021-01-21 出版日期:2021-09-12 网络出版日期:2021-03-10
  • 通讯作者: 张玉先
  • 作者简介:张明聪, E-mail: zhangmingcong@163.com|何松榆, E-mail: 1274167812@qq.com
  • 基金资助:
    国家重点研发计划项目“大田经济作物优质丰产的生理基础与调控”(2018YFD1000905);中国博士后科学基金项目(2016M591561);黑龙江省自然科学基金项目(C2016042);黑龙江省应用技术研究与开发计划项目(GA19B101-02);黑龙江省农垦总局重点科研计划项目(HKKY190206-01);黑龙江八一农垦大学博士科研启动基金项目(XYB2014-04)

Effects of exogenous melatonin on morphology, photosynthetic physiology, and yield of spring soybean variety Suinong 26 under drought stress

ZHANG Ming-Cong1(), HE Song-Yu1(), QIN Bin1, WANG Meng-Xue1, JIN Xi-Jun1, REN Chun-Yuan1, WU Yao-Kun2, ZHANG Yu-Xian1,*   

  1. 1College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China
    2Daqing Branch of Heilongjiang Academy of Agricultural Sciences, Daqing 163316, Heilongjiang, China
  • Received:2020-07-11 Accepted:2021-01-21 Published:2021-09-12 Published online:2021-03-10
  • Contact: ZHANG Yu-Xian
  • Supported by:
    National Key Research and Development Program of China “Physiological Basis and Agronomic Management for High-quality and High-yield of Field Cash Crops”(2018YFD1000905);China Postdoctoral Science Foundation(2016M591561);Natural Science Foundation of Heilongjiang Province(C2016042);Applied Technology Research and Development Project of Heilongjiang Province(GA19B101-02);Key Scientific Research Projects of Heilongjiang Farms and Land Reclamation administration(HKKY190206-01);Doctoral Scientific Research Start-up Foundation of Heilongjiang Bayi Agricultural University(XYB2014-04)

RichHTML

25

PDF

576

摘要：

苗期干旱是影响东北大豆生长的主要因素之一, 研究外源褪黑素对大豆苗期抗旱的调控效应具有重要意义。试验于2018—2019年进行, 以‘绥农26’为材料, 正常浇灌营养液处理为对照(CK), 研究了干旱胁迫(D)、干旱胁迫+叶面喷施褪黑素(LM)和干旱胁迫+浇灌褪黑素(RM)处理对大豆苗期生理指标和产量的影响。结果表明, 与干旱胁迫(D)处理相比, 施用褪黑素处理(LM和RM)提高干旱胁迫下大豆气体交换参数、苗期各器官干物质积累量、叶片中抗氧化酶活性和可溶性蛋白含量。其中, V2期和V4期的净光合速率、气孔导度以及V4期蒸腾速率、胞间二氧化碳浓度均显著提高(P<0.05); RM处理POD和CAT均显著增加(P<0.05); V2期和V4期干旱后第8天可溶性蛋白含量增幅显著(P<0.05)。与D处理相比, V2和V4期LM处理大豆产量年均增加24.2% (P<0.05)和19.1%, RM处理年均增加38.6% (P<0.05)和56.3% (P<0.05), 其中, 与LM处理相比, RM处理产量年均增加11.6%和31.3% (P<0.05)。表明, 应用褪黑素提高干旱胁迫下大豆产量, 其中浇灌褪黑素处理产量高于叶面喷施处理。

关键词: 干旱胁迫, 褪黑素, 大豆, 生长发育, 产量

Abstract:

It is of great significance to study the mechanism of improving the drought resistance ability of soybean seedlings for the growth of soybean seedlings in Northeast China. This experiment was conducted using ‘Suinong 26’ as the experimental material with four treatments to investigate the physiological indexes and yield of soybean under drought stress, including the normal nutrient solution (CK), the drought stress treatment (D), the drought stress + foliar spraying melatonin treatment (LM), and the drought stress + irrigation melatonin treatment (RM). The results indicated that the gas exchange parameters, the dry matter accumulation of organ at seedling stage, the activities of antioxidant enzyme, and the content of soluble protein were increased under LM and RM compared with D. The net photosynthetic rate and stomatal conductance were significantly increased at V2 and V4 stages, and the transpiration rate and intercellular carbon dioxide concentration were significantly increased at V4 stage with P < 0.05; POD and CAT of RM were significantly increased at P < 0.05; the content of soluble protein was significantly increased after drought stress at V2 and V4 stages at P < 0.05. Compared with D, the yield of soybean with LM at V2 and V4 stages was increased by 24.2% and 19.1% at P < 0.05, RM increased by 38.6% and 56.3% at P < 0.05. Compared with LM, the yield of soybean with RM at V2 and V4 stages was increased by 11.6% and 31.3% at P < 0.05. These results revealed that the application of melatonin increased the yield of soybean under drought stress and the yield of soybean with irrigation melatonin treatment was higher than that of foliar spraying melatonin treatment.

Key words: drought stress, melatonin, soybean, growth and development, yield

表1

外源褪黑素对干旱胁迫下大豆株高和叶面积的影响"

指标
Index 		年份
Year 		处理
Treatment 		V2 V4
4 d 8 d 4 d 8 d
株高
Plant height (cm) 		2018 CK 16.76±0.85 a 18.33±0.40 a 21.10±2.55 a 27.63±1.62 a
D 14.87±0.55 b 15.36±0.31 c 17.360±1.05 b 24.03±1.90 b
LM 16.03±0.41 a 16.27±0.41 b 17.80±1.99 ab 25.26±0.64 ab
RM 16.27±0.45 a 16.56±0.61 b 19.20±0.96 ab 25.33±0.76 ab
2019 CK 17.67±0.91 a 18.47±1.17 a 23.87±0.42 ab 30.00±1.87 a
D 15.23±0.75 b 15.77±0.40 a 20.47±0.06 c 25.37±2.41 b
LM 17.30±1.75 ab 17.43±1.12 a 22.90±1.31 b 29.00±0.36 a
RM 16.93±1.08 ab 17.03±2.45 a 24.67±1.04 a 25.77±0.64 b
叶面积
Leaf area (mm2) 		2018 CK 4308.4±166 a 5188.4±123 a 8601.8±157 a 9825.2±173 a
D 2735.5±223 c 3674.9±137 d 5728.9±157 c 6121.5±98 c
LM 3412.1±160 b 4404.0±170 c 5775.9±152 c 6410.2±156 b
RM 3505.1±134 b 4820.9±117 b 6247.5±216 b 6174.6±96 bc
2019 CK 4115.2±284 a 5246.1±111 a 9143.6±282 a 13,317.6±153 a
D 2708.2±131 c 2924.1±137 c 5749.3±143 c 6664.6±216 b
LM 3312.1±183 b 3442.4±150 b 7290.5±190 b 7575.9±203 b
RM 3521.4±124 b 3655.2±152 b 7398.0±224 b 7727.0±154 b

表2

外源褪黑素对干旱胁迫下大豆根系形态指标的影响"

指标
Index 		年份
Year 		处理
Treatment 		V2 V4
4 d 8 d 4 d 8 d
根长
Root length (cm) 		2018 CK 24.16±0.31 a 25.90±0.43 c 45.67±1.72 a 54.73±5.03 b
D 24.86±1.32 a 27.20±0.36 b 46.43±3.09 a 58.30±1.90 ab
LM 25.50±0.60 a 27.80±0.36 ab 46.76±5.81 a 64.33±4.20 a
RM 25.80±1.34 a 28.36±0.41 a 48.30±4.27 a 62.16±1.42 a
2019 CK 23.23±2.10 b 24.43±1.90 a 52.27±7.92 a 58.47±1.22 a
D 25.03±0.95 ab 25.53±1.16 a 63.07±12.0 a 60.83±2.06 a
LM 25.87±2.08 ab 26.80±2.91 a 63.13±1.52 a 68.40±6.09 a
RM 26.73±1.23 a 26.10±1.05 a 62.53±4.77 a 64.63±8.64 a
根体积
Root volume (cm3) 		2018 CK 9.89±0.05 a 10.22±0.09 a 18.63±0.05 c 19.01±0.01 c
D 9.53±0.06 cd 9.89±0.05 bc 18.30±0.02 d 18.79±0.02 d
LM 9.57±0.09 bc 9.92±0.06 b 18.35±0.03 de 18.86±0.06 de
RM 9.57±0.08 bcd 9.95±0.04 b 18.40±0.02 e 18.93±0.02 e
2019 CK 9.67±0.05 b 9.81±0.03 cd 19.79±0.06 a 20.16±0.06 a
D 9.50±0.03 d 9.72±0.06 d 19.66±0.05 b 19.88±0.07 b
LM 9.59±0.08 bcd 9.76±0.05 d 19.68±0.05 b 19.91±0.08 b
RM 9.61±0.04 bc 9.75±0.04 d 19.69±0.09 b 19.95±0.07 b
根表面积
Root surface area (m2) 		2018 CK 0.065±0.02 a 0.074±0.02 a 0.091±0.02 b 0.102±0.03 b
D 0.054±0.01 cd 0.062±0.01 c 0.079±0.01 d 0.092±0.01 d
LM 0.057±0.02 bc 0.068±0.01 b 0.084±0.02 cd 0.095±0.01 cd
RM 0.061±0.02 ab 0.068±0.02 b 0.086±0.02 c 0.097±0.02 cd
2019 CK 0.059±0.02 ab 0.068±0.01 b 0.112±0.03 a 0.122±0.01 a
D 0.043±0.01 e 0.059±0.02 c 0.088±0.01 c 0.099±0.02 bc
LM 0.050±0.01 d 0.062±0.01 c 0.091±0.02 bc 0.105±0.02 b
RM 0.052±0.01 cd 0.062±0.01 c 0.095±0.01 b 0.105±0.03 b

表3

外源褪黑素对干旱胁迫下大豆苗期干物质积累的影响"

指标
Index 		年份
Year 		处理
Treatment 		V2 V4
4 d 8 d 4 d 8 d
叶重
Leaf weight
(g) 		2018 CK 1.16±0.05 a 1.33±0.08 a 1.27±0.17 a 1.81±1.62 a
D 0.81±0.03 c 0.98±0.02 b 0.61±0.03 c 0.80±1.90 b
LM 0.99±0.02 b 1.10±0.09 b 0.99±0.05 b 0.81±0.64 b
RM 1.03±0.05 b 1.11±0.08 b 1.07±0.11 b 0.93±0.76 b
2019 CK 1.11±0.21 a 1.09±0.03 a 2.03±0.21 a 2.82±0.31 a
D 0.47±0.08 c 0.82±0.05 c 1.39±0.09 c 1.73±0.24 b
LM 0.61±0.01 bc 0.89±0.01 b 1.64±0.13 bc 2.04±0.61 b
RM 0.72±0.10 b 0.95±0.05 b 1.92±0.21 ab 2.14±0.32 ab
茎重
Stem weight (g) 		2018 CK 1.05±0.06 a 1.23±0.09 a 1.18±0.28 a 1.47±5.03 a
D 0.78±0.01 b 0.87±0.06 c 0.63±0.03 b 0.57±1.9 c
LM 0.81±0.02 b 1.04±0.09 bc 0.60±0.03 b 0.88±4.2 b
RM 0.83±0.01 b 1.09±0.13 ab 0.71±0.04 b 0.94±1.42 b
2019 CK 0.62±0.10 a 1.01±0.24 a 1.49±0.09 a 2.08±0.19 a
D 0.44±0.02 b 0.39±0.06 c 0.91±0.02 b 1.37±0.25 b
LM 0.51±0.01 b 0.53±0.04 bc 1.33±0.09 a 1.53±0.13 b
RM 0.52±0.01 b 0.64±0.07 b 1.43±0.16 a 1.62±0.19 b
根重
Root weight (g) 		2018 CK 1.43±0.03 a 1.68±0.06 a 1.75±0.25 a 2.28±0.24 a
D 1.25±0.05 c 1.34±0.05 c 1.22±0.03 b 1.24±0.05 c
LM 1.32±0.03 b 1.48±0.03 b 1.28±0.07 b 1.61±0.03 b
RM 1.35±0.01 b 1.49±0.02 b 1.56±0.05 a 1.65±0.03 b
2019 CK 1.21±0.19 a 1.31±0.03 a 2.31±0.05 a 3.19±0.40 a
D 0.64±0.14 b 1.03±0.02 b 2.05±0.01 b 2.26±0.23 b
LM 0.81±0.06 b 1.25±0.07 a 2.31±0.03 a 2.86±0.17 a
RM 0.87±0.12 b 1.27±0.08 a 2.29±0.23 a 2.98±0.31 a
总重
Total weight (g) 		2018 CK 3.65±0.11 a 4.25±0.12 a 4.21±0.47 a 5.55±0.81 a
D 2.85±0.01 c 3.20±0.01 c 2.46±0.09 c 2.61±0.15 b
LM 3.12±0.01 b 3.63±0.01 b 2.91±0.73 bc 3.30±0.06 b
RM 3.22±0.06 b 3.69±0.06 b 3.33±0.19 b 3.39±0.11 b
2019 CK 3.34±0.64 a 3.01±0.15 a 5.84±0.17 a 8.10±0.53 a
D 1.49±0.10 c 2.29±0.09 c 4.36±0.11 b 5.36±0.66 c
LM 1.94±0.14 bc 2.67±0.08 b 5.28±0.19 ab 6.44±0.61 bc
RM 2.24±0.77 b 2.75±0.12 b 5.64±0.59 ab 6.75±0.82 b

图1

外源褪黑素对干旱胁迫下大豆V2期净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)和胞间CO2浓度(Ci)的影响(2019年) 柱上标以不同小写字母表示处理间在0.05水平差异显著。处理同表1。 "

图2

外源褪黑素对干旱胁迫下大豆V4期净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)和胞间CO2浓度(Ci)的影响(2019年) 柱上标以不同小写字母表示处理间在0.05水平差异显著。处理同表1。 "

图3

外源褪黑素对干旱胁迫下大豆超氧化物歧化酶(SOD)活性的影响 V2: 第二节龄期; V4: 第四节龄期。柱上标以不同小写字母表示处理间在0.05水平差异显著。处理同表1。 "

图4

外源褪黑素对干旱胁迫下大豆过氧化物酶(POD)活性的影响 V2: 第二节龄期; V4: 第四节龄期。柱上标以不同小写字母表示处理间在0.05水平差异显著。处理同表1。 "

图5

外源褪黑素对干旱胁迫下大豆过氧化氢酶(CAT)活性的影响 V2: 第二节龄期; V4: 第四节龄期。柱上标以不同小写字母表示处理间在0.05水平差异显著。处理同表1。 "

图6

外源褪黑素对干旱胁迫下大豆抗坏血酸过氧化物酶(APX)活性的影响 V2: 第二节龄期; V4: 第四节龄期。柱上标以不同小写字母表示处理间在0.05水平差异显著。处理同表1。 "

图7

外源褪黑素对干旱胁迫下大豆超氧阴离子(O2-)的影响 V2: 第二节龄期; V4: 第四节龄期。柱上标以不同小写字母表示处理间在0.05水平差异显著。处理同表1。 "

图8

外源褪黑素对干旱胁迫下大豆过氧化氢(H2O2)的影响 V2: 第二节龄期; V4: 第四节龄期。柱上标以不同小写字母表示处理间在0.05水平差异显著。处理同表1。 "

图9

外源褪黑素对干旱胁迫下大豆丙二醛(MDA)含量的影响 V2: 第二节龄期; V4: 第四节龄期。柱上标以不同小写字母表示处理间在0.05水平差异显著。处理同表1。 "

图10

外源褪黑素对干旱胁迫下大豆可溶性蛋白含量的影响 V2: 第二节龄期; V4: 第四节龄期。柱上标以不同小写字母表示处理间在0.05水平差异显著。处理同表1。 "

表4

外源褪黑素对干旱胁迫下大豆产量及其构成因素的影响"

时期
Stage 		处理
Treatment 		节数
Pitch number 		单株荚数
Pods per plant 		单株粒数
Seeds per plant 		百粒重
100-grain weight (g) 		单株产量
Yield per plant (g)
2018 V2 CK 18.5±1.35 a 26.4±6.65 a 70.7±18.38 a 20.31±0.16 a 14.36±3.73 a
D 15.1±1.37 c 18.0±4.26 b 45.6±10.13 b 18.27±0.19 c 8.34±1.85 c
LM 16.0±0.94 bc 18.8±3.93 b 50.6±10.46 b 19.91±0.13 b 10.07±2.08 bc
RM 17.0±1.41 b 21.6±7.72 ab 54.7±20.83 b 20.13±0.02 ab 11.01±2.19 b
V4 CK 17.9±0.87 a 26.8±6.37 a 72.3±21.80 a 19.22±0.06 a 13.90±4.19 a
D 15.2±1.13 c 13.7±3.71 c 35.0±9.97 c 17.47±0.09 d 6.09±1.75 c
LM 16.1±1.19 bc 17.2±3.73 c 44.1±7.05 bc 18.49±0.12 c 8.15±1.30 c
RM 16.3±1.25 b 21.8±5.55 b 56.6±15.90 b 18.97±0.02 b 10.73±3.01 b
2019 V2 CK 18.0±1.41 a 24.2±4.05 a 67.8±12.48 a 20.48±0.04 a 13.78±2.54 a
D 15.8±1.62 b 17.0±3.83 b 45.4±7.59 c 17.68±0.05 d 8.03±1.34 c
LM 16.6±0.96 b 20.6±3.56 ab 54.5±8.21 bc 18.83±0.01 c 10.26±1.54 b
RM 16.5±1.08 b 23.1±4.77 a 59.5±11.42 ab 19.63±0.13 b 11.68±2.24 b
V4 CK 16.7±1.95 a 19.5±4.09 a 55.8±11.50 a 19.98±0.18 a 11.15±2.29 a
D 15.4±0.70 b 11.3±2.71 c 31.3±11.30 c 16.06±0.05 c 5.91±2.14 c
LM 15.8±0.63 ab 11.8±2.94 c 32.1±7.61 c 18.89±0.06 b 6.14±1.45 c
RM 16.2±0.92 ab 15.1±2.96 b 41.2±7.94 b 19.02±0.13 b 8.03±1.55 b
[1] 李秀芬, 马树庆, 李云峰, 于海, 徐丽萍, 陈凤涛, 张洪伟, 翟墨. 水分胁迫对春大豆出苗和幼苗长势的影响. 生态学杂志, 2020, 39:2602-2609.
Li X F, Ma S Q, Li Y F, Yu H, Xu L P, Chen F T, Zhang H W, Zhai M. Effects of soil water stress on emergence and seeding growth of spring soybean. Chin J Ecol, 2020, 39:2602-2609 (in Chinese with English abstract).
[2] 李琬. 干旱对大豆根系生育的影响及灌溉缓解效应研究进展. 草业学报, 2019, 28(4):192-202.
Li W. Research progress in understanding the effect of drought on growth of the soybean root system and the efficiency of irrigation. Acta Pratac Sin, 2019, 28(4):192-202 (in Chinese with English abstract).
[3] 邹京南, 于奇, 金喜军, 王明瑶, 秦彬, 任春元, 王孟雪, 张玉先. 外源褪黑素对干旱胁迫下大豆鼓粒期生理和产量的影响. 作物学报, 2020, 46:745-758.
Zou J N, Yu Q, Jin X J, Wang M Y, Qin B, Ren C Y, Wang M X, Zhang Y X. Effects of exogenous melatonin on physiology and yield of soybean during seed filling stage under drought stress. Acta Agron Sin, 2020, 46:745-758 (in Chinese with English abstract).
[4] 莫金钢, 马建, 沈勇, 张丽辉, 曲静, 王丕武. 干旱胁迫下大豆抗旱突变体M18苗期生长和生理特性. 中国油料作物学报, 2014, 36:770-776.
Mo J G, Ma J, Shen Y, Zhang L H, Qu J, Wang P W. Growth and physiological characteristics of seedlings of soybean drought-resistant mutant M18 under drought stress. Chin J Oil Crop Sci, 2014, 36:770-776 (in Chinese with English abstract).
[5] 叶君, 邓西平, 王仕稳, 殷俐娜, 陈道钳, 熊炳霖, 王鑫月. 干旱胁迫下褪黑素对小麦幼苗生长、光合和抗氧化特性的影响. 麦类作物学报, 2015, 35:1275-1283.
Ye J, Deng X P, Wang S W, Yin L N, Chen D Q, Xiong B L, Wang X Y. Effects of melatonin on growth, photosynthetic characteristics and antioxidant system in seedling of wheat under drought stress. J Triticeae Crops, 2015, 35:1275-1283 (in Chinese with English abstract).
[6] Li B Y, Feng Y N, Zong Y Z, Zhang D S, Hao X Y, Li P. Elevated CO2-induced changes in photosynthesis, antioxidant enzymes and signal transduction enzyme of soybean under drought stress. Plant Physiol Biochem, 2020, 154:105-114.
doi: 10.1016/j.plaphy.2020.05.039
[7] Chastain D R, Snider J L, Collins G D, Perry C D, Whitaker J, Byrd S A. Water deficit in field-grown Gossypium hirsutum primarily limits net photosynthesis by decreasing stomatal conductance, increasing photorespiration, and increasing the ratio of dark respiration to gross photosynthesis. J Plant Physiol, 2014, 171:1576-1585.
doi: 10.1016/j.jplph.2014.07.014
[8] Reiter R J, Tan D X, Terron M P. Melatonin and its metabolites: new findings regarding their production and their radical scavenging actions. Acta Biochim Pol, 2007, 54:1-9.
doi: 10.18388/abp.2007_3264
[9] Zhang M C, He S Y, Zhan Y C, Qin B, Jin X J, Wang M X, Zhang Y X, Hu G H. Exogenous melatonin reduces the inhibitory effect of osmotic stress on photosynthesis in soybean. PLoS One, 2019, 14:e0226542.
doi: 10.1371/journal.pone.0226542
[10] 巩彪, 史庆华. 园艺作物褪黑素的研究进展. 中国农业科学, 2017, 50:2326-2337.
Gong B, Shi Q H. Review of melatonin in horticultural crops. Sci Agric Sin, 2017, 50:2326-2337 (in Chinese with English abstract).
[11] 蒲玉瑾, 张一璇, 苗灵凤, 杨帆. 常温和低温条件下不同浓度褪黑素对降香黄檀幼苗的生理生态影响. 广西植物[2020-08-30]. http://kns.cnki.net/kcms/detail/45.1134.Q.20200807.1108.006.html.
Pu Y J, Zhang Y X, Miao L F, Yang F. Effects of exogenous melatonin on the eco-physiological characteristics of Dalbergia odorifera seedlings under ambient and low temperatures. Guihaia, [2020-08-30]. http://kns.cnki.net/kcms/detail/45.1134.Q.20200 807.1108.006.html (in Chinese with English abstract).
[12] Zou J N, Jin X J, Zhang Y X, Ren C Y, Zhang M C, Wang M X. Effects of melatonin on photosynthesis and soybean seed growth during grain filling under drought stress. Photosynthetica, 2019, 57:512-520.
doi: 10.32615/ps.2019.066
[13] Ye J, Wang S W, Deng X W, Yin L, Xiong B L, Wang X Y. Melatonin increased maize ( Zea mays L.) seedling drought tolerance by alleviating drought-induced photosynthetic inhibition and oxidative damage. Acta Physiol Plant, 2016, 38:38-48.
doi: 10.1007/s11738-015-2059-5
[14] Zhang N, Zhao B, Zhang H J, Weeda S, Yang C, Yang Z C, Ren S X, Guo Y D. Melatonin promotes water-stress tolerance, lateral root formation, and seed germination in cucumber ( Cucumis sativus L.). J Pineal Res, 2013, 54:15-23.
doi: 10.1111/j.1600-079X.2012.01015.x pmid: 22747917
[15] Wang P, Sun X, Li C, Wei Z W, Liang D, Ma F W. Long-term exogenous application of melatonin delays drought-induced leaf senescence in apple. J Pineal Res, 2013, 54:292-302.
doi: 10.1111/jpi.12017
[16] 刘领, 李冬, 马宜林, 王丽君, 赵世民, 周俊学, 申洪涛, 王艳芳. 外源褪黑素对干旱胁迫下烤烟幼苗生长的缓解效应与生理机制研究. 草业学报, 2019, 28(8):95-105.
Liu L, Li D, Ma Y L, Wang L J, Zhao S M, Zhou J X, Shen H T, Wang Y F. Alleviation of drought stress and the physiological mechanisms in tobacco seedings treated with exogenous melatonin. Acta Pratac Sin, 2019, 28(8):95-105 (in Chinese with English abstract).
[17] Cao L, Jin X J, Zhang Y X. Melatonin confers drought stress tolerance in soybean (Glycine max L.) by modulating photosynthesis, osmolytes, and reactive oxygen metabolism. Photosynthetica, 2019, 57:812-819.
doi: 10.32615/ps.2019.100
[18] Gao H, Zhang Z K, Chai H K, Hong K, Cheng N, Yang Y, Wang D N, Yang T, Cao W. Melatonin treatment delays postharvest senescence and regulates reactive oxygen species metabolism in peach fruit. Posth Biol Technol, 2016, 118:103-110.
doi: 10.1016/j.postharvbio.2016.03.006
[19] Zhang M C, Sun W X, Liu Y Y, Luo S G, Zhang J, Wu Q, Wu Z Y, Jiang Y. Timing of N application affects net primary production of soybean with different densities. J Integr Agric, 2014, 13:60345-60347.
[20] 秦彬, 张明聪, 何松榆, 张春宇, 王明瑶, 金喜军, 王孟雪, 张玉先, 胡国华. 褪黑素浸种对大豆种子萌发过程中干旱胁迫的缓解效应. 干旱地区农业研究, 2020, 38(3):192-198.
Qin B, Zhang M C, He S Y, Zhang C Y, Wang M Y, Jin X J, Wang M X, Zhang Y X, Hu G H. Alleviating effect of melatonin soaking on drought stress during soybean seed germination. Agric Res Arid Areas, 2020, 38(3):192-198 (in Chinese with English abstract).
[21] 施海涛. 植物逆境生理学实验指导. 北京: 科学出版社, 2016. pp 57-74.
Shi H T. Plant Adversity Physiology Experiment Guide. Beijing: Science Press, 2016. pp 57-74(in Chinese).
[22] Chaitanya K K, Naithani S C. Role of superoxide, lipid peroxidation and superoxide dismutase in membrane perturbation during loss of viability in seeds of Shorea robusta Gaertn. F. New Phytol, 1994, 126:623-627.
doi: 10.1111/nph.1994.126.issue-4
[23] Mukherjee S P, Choudhuri M A. Implications of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plant, 1983, 58:166-170.
doi: 10.1111/ppl.1983.58.issue-2
[24] 刘文夫, 董守坤, 徐亚会, 李雪凝, 刘丽君. 大豆苗期干旱胁迫对糖分吸收与相关酶活性的影响. 作物杂志, 2014, (3):117-120.
Liu W F, Dong S K, Xu Y H, Li X N, Liu L J. Effects of drought stress on sugar absorption and elated enzyme activities at soybean seedling. Crops, 2014, (3):117-120 (in Chinese with English abstract).
[25] 魏鑫, 倪虹, 张会慧, 王晶英. 外源脱落酸和油菜素内酯对干旱胁迫下大豆幼苗抗旱性的影响. 中国油料作物学报, 2016, 38:605-610.
Wei X, Ni H, Zhang H H, Wang J Y. Effects of exogenous abscisic acid and brassinolide on drought resistance of soybean seedlings. Chin J Oil Crop Sci, 2016, 38:605-610 (in Chinese with English abstract).
[26] Tilden A R, Becker M A, Amma L L, Arciniega J, Mcgaw A K. Melatonin production in an aerobic photosynthetic bacterium: an evolutionarily early association with darkness. J Pineal Res, 1997, 22:102-106.
pmid: 9181522
[27] Wang P, Sun X, Li C, Wei Z W, Liang D, Ma F W. Long-term exogenous application of melatonin delays drought-induced leaf senescence in apple. J Pineal Res, 2013, 54:292-302.
doi: 10.1111/jpi.12017
[28] Wei W, Li Q T, Chu Y N, Russel J R, Yu X M, Zhu D H, Zhang W K, Ma B, Lin Q, Zhang J S, Chen S Y. Melatonin enhances plant growth and abiotic stress tolerance in soybean plants. J Exp Bot, 2015, 66:695-707.
doi: 10.1093/jxb/eru392 pmid: 25297548
[29] Nazar R, Umar S, Khan N A, Sareer O. Salicylic acid supplementation improves photosynthesis and growth in mustard through changes in proline accumulation and ethylene formation under drought stress. South Afr J Bot, 2015, 98:84-94.
doi: 10.1016/j.sajb.2015.02.005
[30] Kobra M, Yahya E, Mohammad P. Effect of silicon on photosynthetic gas exchange, photosynthetic pigments, cell membrane stability and relative water content of different wheat cultivars under drought stress conditions. J Plant Nutr, 2016, 39:1001-1015.
doi: 10.1080/01904167.2015.1109108
[31] 李耕, 高辉远, 赵斌, 董树亭, 张吉旺, 杨吉顺, 王敬锋, 刘鹏. 灌浆期干旱胁迫对玉米叶片光系统活性的影响. 作物学报, 2009, 35:1916-1922.
Li G, Gao H Y, Zhao B, Dong S T, Zhang J W, Yang J S, Wang J F, Liu P. Effects of drought stress on activity of photosystems in leaves of maize at grain filling stage. Acta Agron Sin, 2009, 35:1916-1922 (in Chinese with English abstract).
[32] 耿庆伟. 臭氧胁迫叠加温光逆境对葡萄叶片光合性能的影响. 山东农业大学硕士学位论文, 山东泰安, 2017.
Geng Q W. Effect of High Ozone Plus Temperature and Light Stress on Photosynthetic Characteristics of Grape Leaves. MS Thesis of Shandong Agricultural University, Tai’an, Shandong, China, 2017 (in Chinese with English abstract).
[33] 杨新元. 外源褪黑素对干旱胁迫下向日葵幼苗生长、光合及抗氧化系统的影响. 华北农学报, 2019, 34(4):113-121.
Yang X Y. Effects of exogenous melatonin on growth, photosynthesis and antioxidant system of sunflower seedling under drought stress. Acta Agric Boreali-Sin, 2019, 34(4):113-121 (in Chinese with English abstract).
[34] Li C, Tan D X, Liang D, Chang C, Jia D F, Ma F W. Melatonin mediates the regulation of ABA metabolism, free-radical scavenging, and stomatal behaviour in two Malus species under drought stress. J Exp Bot, 2015, 66:669-680.
doi: 10.1093/jxb/eru476
[35] 李冬, 王艳芳, 申洪涛, 马宜林, 王丽君, 赵世民, 刘领. 外源MT和EBR对干旱胁迫下烤烟幼苗的缓解效应. 中国烟草学报, 2019, 25(5):77-85.
Li D, Wang Y F, Shen H T, Ma Y L, Wang L J, Zhao S M, Liu L. Alleviation effects of exogenous melatonin and 2,4-epibrassinolide on flue-cured tobacco seedlings under drought stress. Acta Tab Sin, 2019, 25(5):77-85 (in Chinese with English abstract).
[36] 万林, 李张开, 李素, 刘丽欣, 马霓, 张春雷. 外源独脚金内酯对油菜苗期干旱胁迫的缓解效应. 中国油料作物学报, 2020, 42:461-471.
Wan L, Li Z K, Li S, Liu L X, Ma N, Zhang C L. Alleviation effects of exogenous strigolactone on growth of Brassica napus L. seedling under drought stress. Chin J Oil Crop Sci, 2020, 42:461-471 (in Chinese with English abstract).
[37] Tan D X, Manchester L C, Esteban-zubero E, Zhou Z, Reiter R J. Melatonin as a potent and inducible endogenous antioxidant: synthesis and metabolism. Molecules, 2015, 20:18886-18906.
doi: 10.3390/molecules201018886
[38] 厉恩茂, 李敏, 安秀红, 陈艳辉, 李燕青, 李壮, 程存刚. 叶面喷施褪黑素对干旱胁迫下苹果抗旱生理生化指标的影响. 中国南方果树, 2019, 48(4):95-98.
Li E M, Li M, An X H, Chen Y H, Li Y Q, Li Z, Cheng C G. Effects of melatonin on physiological and biochemical indexes of apple under drought stress. Fruit Trees Southern China, 2019, 48(4):95-98 (in Chinese with English abstract).
[39] 刘仕翔, 黄益宗, 罗泽娇, 黄永春, 保琼莉, 王培培, 袁彪, 李文华. 外源褪黑素处理对镉胁迫下水稻种子萌发的影响. 农业环境科学学报, 2016, 35:1034-1041.
Liu S X, Huang Y Z, Luo Z J, Huang Y C, Bao Q L, Wang P P, Yuan B, Li W H. Effects of exogenous melatonin on germination of rice seeds under Cd stresses. J Agro-Environ Sci, 2016, 35:1034-1041 (in Chinese with English abstract).
[40] 张娜. 褪黑素处理对渗透胁迫下黄瓜种子萌发及幼苗生长的影响及其分子机制. 中国农业大学博士学位论文, 北京, 2014.
Zhang N. Regulation of Melatonin on Germination and Seedling Growth under Osmotic Stress in Cucumber. PhD Dissertation of China Agricultural University, Beijing, China, 2014 (in Chinese with English abstract).
[41] 曹林, 吴玉环, 章艺, 郭怡, 肖有铁, 郦枫, 马丽, 徐根娣, 刘鹏. 外源水杨酸对铝胁迫下菊芋光合特性及耐铝性的影响. 水土保持学报, 2015, 29(4):260-266.
Cao L, Wu Y H, Zhang Y, Guo Y, Xiao Y T, Li F, Ma L, Xu G D, Liu P. Effect of exogenous salicylic acid on photosynthetic characteristics and aluminum tolerance of helianthus tuberosus under aluminum stress. J Soil Water Conserv, 2015, 29(4):260-266 (in Chinese with English abstract).
[42] 于奇, 曹亮, 金喜军, 邹京南, 王孟雪, 张明聪, 任春元, 张玉先. 低温胁迫下褪黑素对大豆种子萌发的影响. 大豆科学, 2019, 38:56-62.
Yu Q, Cao L, Jin X J, Zou J N, Wang M X, Zhang M C, Ren C Y, Zhang Y X. Effects of melatonin on seed germination of soybean under low temperature stress. Soybean Sci, 2019, 38:56-62 (in Chinese with English abstract).
[43] 赫传杰. 外源褪黑素对干旱胁迫下樟子松的缓解效应. 沈阳农业大学学报, 2020, 51:279-286.
He C J. Alleviating effect of exogenous melatonin on Pinus sylvestris var. mongolica under drought stress. J Shenyang Agric Univ, 2020, 51:279-286 (in Chinese with English abstract).
[44] Liu J, Wang W, Wang L. Exogenous melatonin improves seedling health index and drought tolerance in tomato. Plant Growth Regul, 2015, 77:317-326.
doi: 10.1007/s10725-015-0066-6
[1] 陈玲玲, 李战, 刘亭萱, 谷勇哲, 宋健, 王俊, 邱丽娟. 基于783份大豆种质资源的叶柄夹角全基因组关联分析[J]. 作物学报, 2022, 48(6): 1333-1345.
[2] 王丹, 周宝元, 马玮, 葛均筑, 丁在松, 李从锋, 赵明. 长江中游双季玉米种植模式周年气候资源分配与利用特征[J]. 作物学报, 2022, 48(6): 1437-1450.
[3] 王旺年, 葛均筑, 杨海昌, 阴法庭, 黄太利, 蒯婕, 王晶, 汪波, 周广生, 傅廷栋. 大田作物在不同盐碱地的饲料价值评价[J]. 作物学报, 2022, 48(6): 1451-1462.
[4] 颜佳倩, 顾逸彪, 薛张逸, 周天阳, 葛芊芊, 张耗, 刘立军, 王志琴, 顾骏飞, 杨建昌, 周振玲, 徐大勇. 耐盐性不同水稻品种对盐胁迫的响应差异及其机制[J]. 作物学报, 2022, 48(6): 1463-1475.
[5] 杨欢, 周颖, 陈平, 杜青, 郑本川, 蒲甜, 温晶, 杨文钰, 雍太文. 玉米-豆科作物带状间套作对养分吸收利用及产量优势的影响[J]. 作物学报, 2022, 48(6): 1476-1487.
[6] 陈静, 任佰朝, 赵斌, 刘鹏, 张吉旺. 叶面喷施甜菜碱对不同播期夏玉米产量形成及抗氧化能力的调控[J]. 作物学报, 2022, 48(6): 1502-1515.
[7] 李祎君, 吕厚荃. 气候变化背景下农业气象灾害对东北地区春玉米产量影响[J]. 作物学报, 2022, 48(6): 1537-1545.
[8] 王炫栋, 杨孙玉悦, 高润杰, 余俊杰, 郑丹沛, 倪峰, 蒋冬花. 拮抗大豆斑疹病菌放线菌菌株的筛选和促生作用及防效研究[J]. 作物学报, 2022, 48(6): 1546-1557.
[9] 石艳艳, 马志花, 吴春花, 周永瑾, 李荣. 垄作沟覆地膜对旱地马铃薯光合特性及产量形成的影响[J]. 作物学报, 2022, 48(5): 1288-1297.
[10] 于春淼, 张勇, 王好让, 杨兴勇, 董全中, 薛红, 张明明, 李微微, 王磊, 胡凯凤, 谷勇哲, 邱丽娟. 栽培大豆×半野生大豆高密度遗传图谱构建及株高QTL定位[J]. 作物学报, 2022, 48(5): 1091-1102.
[11] 李阿立, 冯雅楠, 李萍, 张东升, 宗毓铮, 林文, 郝兴宇. 大豆叶片响应CO2浓度升高、干旱及其交互作用的转录组分析[J]. 作物学报, 2022, 48(5): 1103-1118.
[12] 王霞, 尹晓雨, 于晓明, 刘晓丹. 干旱锻炼对B73自交后代当代干旱胁迫记忆基因表达及其启动子区DNA甲基化的影响[J]. 作物学报, 2022, 48(5): 1191-1198.
[13] 彭西红, 陈平, 杜青, 杨雪丽, 任俊波, 郑本川, 罗凯, 谢琛, 雷鹿, 雍太文, 杨文钰. 减量施氮对带状套作大豆土壤通气环境及结瘤固氮的影响[J]. 作物学报, 2022, 48(5): 1199-1209.
[14] 雷新慧, 万晨茜, 陶金才, 冷佳俊, 吴怡欣, 王家乐, 王鹏科, 杨清华, 冯佰利, 高金锋. 褪黑素与2,4-表油菜素内酯浸种对盐胁迫下荞麦发芽与幼苗生长的促进效应[J]. 作物学报, 2022, 48(5): 1210-1221.
[15] 闫晓宇, 郭文君, 秦都林, 王双磊, 聂军军, 赵娜, 祁杰, 宋宪亮, 毛丽丽, 孙学振. 滨海盐碱地棉花秸秆还田和深松对棉花干物质积累、养分吸收及产量的影响[J]. 作物学报, 2022, 48(5): 1235-1247.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2