作物学报 ›› 2022, Vol. 48 ›› Issue (8): 1948-1956.doi: 10.3724/SP.J.1006.2022.12049
王穆穆1(), 何艳芳2, 郑永胜1, 王晖1, 王丽媛1, 王东建1, 张晗1, 李汝玉1,*()
WANG Mu-Mu1(), HE Yan-Fan2, ZHENG Yong-Sheng1, WANG Hui1, WANG Li-Yuan1, WANG Dong-Jian1, ZHANG Han1, LI Ru-Yu1,*()
摘要:
落粒性是水稻的重要性状之一, 是野生稻种子传播和后代繁衍的重要保障, 但是容易落粒在栽培稻的生产中会造成产量损失。目前, 在水稻中已经克隆了一些落粒相关基因, 但是这些基因并不能解释水稻落粒的全部表型变异。本研究从白谷(Pei-kuh)和普通野生稻(W1944)构建的重组自交系中选取落粒性强且不含有SH4基因的材料E6-5, 与Pei-kuh进行杂交构建分离群体, 将以前检测到位于8号染色体的落粒QTL精细定位于26.4 kb的区间内, 并把该位点的落粒基因命名为SHATTERING8 (SH8)。该区段共有4个编码基因, Pei-kuh和E6-5的测序比对发现LOC_Os08g41950基因在编码区有3个碱基的差异, 同时实时定量PCR分析表明该基因在离层部位表达, 因此我们把LOC_Os08g41950作为SH8的目标候选基因。为了验证LOC_Os08g41950是否参与水稻落粒的调控, 我们利用来自E6-5的LOC_Os08g41950编码序列构建了过表达载体并遗传转化Pei-huh。落粒表型鉴定结果表明, 与Pei-huh相比, 转基因植株的落粒性明显增强, 不仅种子脱落所需的拉力值显著降低, 而且还具有更光滑的离层断裂面。本研究有助于促进水稻驯化和水稻落粒调控机制的研究。
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