欢迎访问作物学报，今天是
作物学报 ›› 2022, Vol. 48 ›› Issue (12): 3018-3028.doi: 10.3724/SP.J.1006.2022.13074
秦永田1,2(
), 陈黎霞3, 汤继华1, 陈建辉1, 马拴红1, 张雪海1, 丁冬1(), 付志远1()
QIN Yong-Tian1,2(), CHEN Li-Xia3, TANG Ji-Hua1, CHEN Jian-Hui1, MA Shuan-Hong1, ZHANG Xue-Hai1, DING Dong1(), FU Zhi-Yuan1()
摘要：
汞污染已成为威胁全球作物生产的主要重金属污染源之一。microRNA (miRNA)是调控植物生长发育和非生物胁迫响应的重要因子, 但其在单子叶植物汞胁迫响应中的作用尚不明确。为挖掘响应汞胁迫的关键miRNA, 本研究对B73和郑58 (Zheng 58, Z58)自交系幼苗在HgCl2处理后的表型和差异表达的miRNA进行了分析。结果表明, B73对汞胁迫较郑58更敏感, miRNA166l是B73和Z58中共同鉴定到的下调表达的miRNA。为进一步验证miRNA166在汞胁迫中的作用, 利用STTM (short tandem target mimic)技术获得了STTM165/166拟南芥转基因稳定株系。STTM166稳定系在HgCl2处理后的表型与玉米幼苗在汞胁迫处理后的表型类似, 叶片失绿萎蔫、根长变短。本研究证明miRNA166在玉米汞胁迫响应中有重要调控作用, 对其作用机制进行更深入的研究有重要意义。
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